Gordonia phage Doggs

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3WI00|A0A7G3WI00_9CAUD Uncharacterized protein OS=Gordonia phage Doggs OX=2743911 GN=59 PE=4 SV=1
MM1 pKa = 7.4YY2 pKa = 9.77PPDD5 pKa = 3.78HH6 pKa = 6.9VGPDD10 pKa = 3.69EE11 pKa = 6.2DD12 pKa = 5.78DD13 pKa = 3.29GTMPDD18 pKa = 3.26NVADD22 pKa = 4.47LRR24 pKa = 11.84DD25 pKa = 3.53AVIGRR30 pKa = 11.84RR31 pKa = 11.84IVRR34 pKa = 11.84AEE36 pKa = 3.82QTDD39 pKa = 3.91FRR41 pKa = 11.84ADD43 pKa = 3.16DD44 pKa = 3.94FAASEE49 pKa = 4.24HH50 pKa = 4.82TAYY53 pKa = 9.74WSSLDD58 pKa = 3.59GNGLVLTLDD67 pKa = 3.67DD68 pKa = 4.0GRR70 pKa = 11.84RR71 pKa = 11.84VALVDD76 pKa = 3.53TSSCCAYY83 pKa = 9.86TDD85 pKa = 3.66LQEE88 pKa = 5.16FFLNPDD94 pKa = 3.57LVDD97 pKa = 3.65HH98 pKa = 6.8VITGVATTDD107 pKa = 4.75GYY109 pKa = 6.7TTWHH113 pKa = 6.94IYY115 pKa = 11.07ADD117 pKa = 3.92LGDD120 pKa = 3.57IMRR123 pKa = 11.84LKK125 pKa = 10.83VGWSAGNPFYY135 pKa = 10.89YY136 pKa = 10.41GYY138 pKa = 10.91GFDD141 pKa = 4.0IGVSTTIEE149 pKa = 4.05GSLALPEE156 pKa = 4.24IEE158 pKa = 5.22GPASS162 pKa = 3.11

Molecular weight: 17.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3VAK6|A0A7G3VAK6_9CAUD Capsid maturation protease OS=Gordonia phage Doggs OX=2743911 GN=4 PE=3 SV=1
MM1 pKa = 7.59AVTRR5 pKa = 11.84NIGAALPLCPQPPHH19 pKa = 5.0QTGDD23 pKa = 2.86GGFVVRR29 pKa = 11.84GEE31 pKa = 4.16VDD33 pKa = 3.27MQSAGVLTPDD43 pKa = 4.55GVDD46 pKa = 3.42SLITGAEE53 pKa = 4.01AARR56 pKa = 11.84LCGVSTVTIRR66 pKa = 11.84KK67 pKa = 4.83WTHH70 pKa = 5.56RR71 pKa = 11.84GYY73 pKa = 11.05VDD75 pKa = 3.98SKK77 pKa = 11.35GAGQRR82 pKa = 11.84LAVAGRR88 pKa = 11.84DD89 pKa = 3.36RR90 pKa = 11.84QGRR93 pKa = 11.84NLYY96 pKa = 10.85RR97 pKa = 11.84MIDD100 pKa = 3.52VAKK103 pKa = 10.38AEE105 pKa = 4.2HH106 pKa = 6.01ATRR109 pKa = 11.84TRR111 pKa = 11.84ARR113 pKa = 11.84RR114 pKa = 11.84SS115 pKa = 3.18

Molecular weight: 12.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

14840

27

1892

231.9

25.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.695 ± 0.695

0.822 ± 0.145

6.193 ± 0.283

5.93 ± 0.336

2.662 ± 0.199

8.477 ± 0.434

1.941 ± 0.201

4.34 ± 0.239

3.032 ± 0.201

8.059 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.133

2.709 ± 0.161

5.728 ± 0.315

3.848 ± 0.353

7.513 ± 0.468

5.889 ± 0.227

6.806 ± 0.306

7.183 ± 0.21

1.934 ± 0.19

2.089 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski