Amino acid dipepetide frequency for Paenibacillus sp. OV191

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.532AlaAla: 9.532 ± 0.115
0.725AlaCys: 0.725 ± 0.02
4.479AlaAsp: 4.479 ± 0.052
5.629AlaGlu: 5.629 ± 0.065
3.471AlaPhe: 3.471 ± 0.046
6.905AlaGly: 6.905 ± 0.068
1.38AlaHis: 1.38 ± 0.026
5.616AlaIle: 5.616 ± 0.061
4.31AlaLys: 4.31 ± 0.055
8.104AlaLeu: 8.104 ± 0.079
2.191AlaMet: 2.191 ± 0.031
2.873AlaAsn: 2.873 ± 0.041
2.667AlaPro: 2.667 ± 0.039
2.569AlaGln: 2.569 ± 0.04
3.266AlaArg: 3.266 ± 0.045
5.077AlaSer: 5.077 ± 0.051
3.275AlaThr: 3.275 ± 0.047
6.653AlaVal: 6.653 ± 0.06
0.981AlaTrp: 0.981 ± 0.026
2.68AlaTyr: 2.68 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.523CysAla: 0.523 ± 0.017
0.128CysCys: 0.128 ± 0.008
0.375CysAsp: 0.375 ± 0.013
0.46CysGlu: 0.46 ± 0.019
0.336CysPhe: 0.336 ± 0.014
0.777CysGly: 0.777 ± 0.022
0.171CysHis: 0.171 ± 0.01
0.524CysIle: 0.524 ± 0.019
0.35CysLys: 0.35 ± 0.013
0.745CysLeu: 0.745 ± 0.02
0.217CysMet: 0.217 ± 0.012
0.264CysAsn: 0.264 ± 0.012
0.375CysPro: 0.375 ± 0.014
0.201CysGln: 0.201 ± 0.009
0.418CysArg: 0.418 ± 0.018
0.598CysSer: 0.598 ± 0.019
0.398CysThr: 0.398 ± 0.016
0.456CysVal: 0.456 ± 0.016
0.091CysTrp: 0.091 ± 0.007
0.264CysTyr: 0.264 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.993AspAla: 3.993 ± 0.049
0.374AspCys: 0.374 ± 0.012
2.409AspAsp: 2.409 ± 0.036
3.791AspGlu: 3.791 ± 0.046
2.205AspPhe: 2.205 ± 0.036
4.162AspGly: 4.162 ± 0.059
1.119AspHis: 1.119 ± 0.025
3.529AspIle: 3.529 ± 0.041
2.866AspLys: 2.866 ± 0.037
4.729AspLeu: 4.729 ± 0.048
1.368AspMet: 1.368 ± 0.027
1.921AspAsn: 1.921 ± 0.04
2.299AspPro: 2.299 ± 0.035
1.869AspGln: 1.869 ± 0.029
2.721AspArg: 2.721 ± 0.041
2.937AspSer: 2.937 ± 0.041
2.502AspThr: 2.502 ± 0.037
3.446AspVal: 3.446 ± 0.043
0.896AspTrp: 0.896 ± 0.025
2.117AspTyr: 2.117 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.079GluAla: 6.079 ± 0.072
0.407GluCys: 0.407 ± 0.015
2.923GluAsp: 2.923 ± 0.045
4.985GluGlu: 4.985 ± 0.066
2.028GluPhe: 2.028 ± 0.033
4.272GluGly: 4.272 ± 0.049
1.572GluHis: 1.572 ± 0.031
4.169GluIle: 4.169 ± 0.048
3.99GluLys: 3.99 ± 0.055
7.238GluLeu: 7.238 ± 0.08
1.944GluMet: 1.944 ± 0.035
2.523GluAsn: 2.523 ± 0.037
2.265GluPro: 2.265 ± 0.035
3.911GluGln: 3.911 ± 0.063
3.973GluArg: 3.973 ± 0.056
3.584GluSer: 3.584 ± 0.04
3.37GluThr: 3.37 ± 0.042
4.048GluVal: 4.048 ± 0.061
1.044GluTrp: 1.044 ± 0.026
1.983GluTyr: 1.983 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.297PheAla: 3.297 ± 0.048
0.336PheCys: 0.336 ± 0.015
2.352PheAsp: 2.352 ± 0.034
2.585PheGlu: 2.585 ± 0.037
1.951PhePhe: 1.951 ± 0.038
3.351PheGly: 3.351 ± 0.049
0.931PheHis: 0.931 ± 0.021
3.148PheIle: 3.148 ± 0.05
2.192PheLys: 2.192 ± 0.036
3.758PheLeu: 3.758 ± 0.061
1.182PheMet: 1.182 ± 0.028
1.744PheAsn: 1.744 ± 0.032
1.726PhePro: 1.726 ± 0.033
1.462PheGln: 1.462 ± 0.03
1.997PheArg: 1.997 ± 0.037
2.954PheSer: 2.954 ± 0.038
2.479PheThr: 2.479 ± 0.039
3.022PheVal: 3.022 ± 0.042
0.574PheTrp: 0.574 ± 0.015
1.523PheTyr: 1.523 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
5.459GlyAla: 5.459 ± 0.063
0.73GlyCys: 0.73 ± 0.024
3.491GlyAsp: 3.491 ± 0.045
4.479GlyGlu: 4.479 ± 0.053
3.308GlyPhe: 3.308 ± 0.05
5.476GlyGly: 5.476 ± 0.08
1.495GlyHis: 1.495 ± 0.031
5.869GlyIle: 5.869 ± 0.068
4.567GlyLys: 4.567 ± 0.059
7.065GlyLeu: 7.065 ± 0.067
2.34GlyMet: 2.34 ± 0.039
2.899GlyAsn: 2.899 ± 0.048
1.966GlyPro: 1.966 ± 0.042
2.68GlyGln: 2.68 ± 0.046
3.392GlyArg: 3.392 ± 0.046
4.881GlySer: 4.881 ± 0.084
4.355GlyThr: 4.355 ± 0.061
5.004GlyVal: 5.004 ± 0.053
1.175GlyTrp: 1.175 ± 0.029
2.868GlyTyr: 2.868 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
1.662HisAla: 1.662 ± 0.034
0.175HisCys: 0.175 ± 0.011
0.957HisAsp: 0.957 ± 0.023
1.334HisGlu: 1.334 ± 0.029
1.128HisPhe: 1.128 ± 0.025
1.504HisGly: 1.504 ± 0.028
0.61HisHis: 0.61 ± 0.024
1.467HisIle: 1.467 ± 0.028
0.89HisLys: 0.89 ± 0.021
2.074HisLeu: 2.074 ± 0.03
0.571HisMet: 0.571 ± 0.015
0.725HisAsn: 0.725 ± 0.02
1.153HisPro: 1.153 ± 0.027
0.743HisGln: 0.743 ± 0.023
0.998HisArg: 0.998 ± 0.025
1.288HisSer: 1.288 ± 0.026
1.076HisThr: 1.076 ± 0.023
1.395HisVal: 1.395 ± 0.026
0.334HisTrp: 0.334 ± 0.013
0.89HisTyr: 0.89 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.133IleAla: 6.133 ± 0.058
0.622IleCys: 0.622 ± 0.02
3.95IleAsp: 3.95 ± 0.047
4.58IleGlu: 4.58 ± 0.054
2.528IlePhe: 2.528 ± 0.043
5.636IleGly: 5.636 ± 0.063
1.517IleHis: 1.517 ± 0.028
4.716IleIle: 4.716 ± 0.064
3.365IleLys: 3.365 ± 0.049
5.58IleLeu: 5.58 ± 0.053
1.768IleMet: 1.768 ± 0.032
2.737IleAsn: 2.737 ± 0.041
3.244IlePro: 3.244 ± 0.038
2.503IleGln: 2.503 ± 0.037
3.77IleArg: 3.77 ± 0.045
4.653IleSer: 4.653 ± 0.053
3.861IleThr: 3.861 ± 0.047
5.338IleVal: 5.338 ± 0.05
0.793IleTrp: 0.793 ± 0.021
2.228IleTyr: 2.228 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
4.502LysAla: 4.502 ± 0.049
0.265LysCys: 0.265 ± 0.014
2.847LysAsp: 2.847 ± 0.044
4.26LysGlu: 4.26 ± 0.057
1.677LysPhe: 1.677 ± 0.035
3.57LysGly: 3.57 ± 0.045
1.205LysHis: 1.205 ± 0.026
3.345LysIle: 3.345 ± 0.046
3.37LysLys: 3.37 ± 0.054
5.813LysLeu: 5.813 ± 0.073
1.65LysMet: 1.65 ± 0.03
2.204LysAsn: 2.204 ± 0.035
2.411LysPro: 2.411 ± 0.037
2.805LysGln: 2.805 ± 0.04
2.993LysArg: 2.993 ± 0.043
3.108LysSer: 3.108 ± 0.048
2.902LysThr: 2.902 ± 0.039
3.648LysVal: 3.648 ± 0.04
0.805LysTrp: 0.805 ± 0.024
1.854LysTyr: 1.854 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
8.125LeuAla: 8.125 ± 0.074
0.794LeuCys: 0.794 ± 0.023
4.985LeuAsp: 4.985 ± 0.059
6.131LeuGlu: 6.131 ± 0.077
4.744LeuPhe: 4.744 ± 0.057
6.483LeuGly: 6.483 ± 0.061
2.061LeuHis: 2.061 ± 0.034
6.663LeuIle: 6.663 ± 0.068
5.383LeuLys: 5.383 ± 0.055
10.877LeuLeu: 10.877 ± 0.103
2.648LeuMet: 2.648 ± 0.042
4.02LeuAsn: 4.02 ± 0.049
4.446LeuPro: 4.446 ± 0.054
4.115LeuGln: 4.115 ± 0.057
4.858LeuArg: 4.858 ± 0.052
7.086LeuSer: 7.086 ± 0.073
5.596LeuThr: 5.596 ± 0.057
6.062LeuVal: 6.062 ± 0.07
1.119LeuTrp: 1.119 ± 0.025
3.284LeuTyr: 3.284 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.143MetAla: 2.143 ± 0.036
0.159MetCys: 0.159 ± 0.009
1.437MetAsp: 1.437 ± 0.024
1.79MetGlu: 1.79 ± 0.032
1.058MetPhe: 1.058 ± 0.021
1.698MetGly: 1.698 ± 0.035
0.53MetHis: 0.53 ± 0.016
2.014MetIle: 2.014 ± 0.037
2.086MetLys: 2.086 ± 0.034
3.046MetLeu: 3.046 ± 0.038
0.848MetMet: 0.848 ± 0.023
1.572MetAsn: 1.572 ± 0.028
1.175MetPro: 1.175 ± 0.028
1.015MetGln: 1.015 ± 0.025
1.347MetArg: 1.347 ± 0.024
1.836MetSer: 1.836 ± 0.03
1.723MetThr: 1.723 ± 0.033
1.701MetVal: 1.701 ± 0.034
0.251MetTrp: 0.251 ± 0.012
0.792MetTyr: 0.792 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.005AsnAla: 3.005 ± 0.053
0.284AsnCys: 0.284 ± 0.014
2.179AsnAsp: 2.179 ± 0.04
2.856AsnGlu: 2.856 ± 0.048
1.43AsnPhe: 1.43 ± 0.032
3.515AsnGly: 3.515 ± 0.056
0.913AsnHis: 0.913 ± 0.021
2.575AsnIle: 2.575 ± 0.036
2.394AsnLys: 2.394 ± 0.04
3.305AsnLeu: 3.305 ± 0.041
1.045AsnMet: 1.045 ± 0.023
1.76AsnAsn: 1.76 ± 0.042
2.088AsnPro: 2.088 ± 0.036
1.544AsnGln: 1.544 ± 0.034
2.26AsnArg: 2.26 ± 0.037
2.295AsnSer: 2.295 ± 0.041
2.049AsnThr: 2.049 ± 0.04
2.715AsnVal: 2.715 ± 0.04
0.642AsnTrp: 0.642 ± 0.02
1.535AsnTyr: 1.535 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
3.323ProAla: 3.323 ± 0.045
0.247ProCys: 0.247 ± 0.011
2.488ProAsp: 2.488 ± 0.036
3.163ProGlu: 3.163 ± 0.04
2.042ProPhe: 2.042 ± 0.033
2.887ProGly: 2.887 ± 0.04
0.894ProHis: 0.894 ± 0.02
2.628ProIle: 2.628 ± 0.038
1.849ProLys: 1.849 ± 0.03
4.041ProLeu: 4.041 ± 0.047
0.985ProMet: 0.985 ± 0.025
1.723ProAsn: 1.723 ± 0.036
1.239ProPro: 1.239 ± 0.028
1.39ProGln: 1.39 ± 0.03
1.428ProArg: 1.428 ± 0.032
2.499ProSer: 2.499 ± 0.035
1.869ProThr: 1.869 ± 0.036
3.195ProVal: 3.195 ± 0.045
0.524ProTrp: 0.524 ± 0.015
1.581ProTyr: 1.581 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.336GlnAla: 3.336 ± 0.047
0.211GlnCys: 0.211 ± 0.011
1.572GlnAsp: 1.572 ± 0.03
2.353GlnGlu: 2.353 ± 0.041
1.64GlnPhe: 1.64 ± 0.027
2.393GlnGly: 2.393 ± 0.039
0.793GlnHis: 0.793 ± 0.02
2.613GlnIle: 2.613 ± 0.044
2.004GlnLys: 2.004 ± 0.034
4.575GlnLeu: 4.575 ± 0.061
1.205GlnMet: 1.205 ± 0.023
1.489GlnAsn: 1.489 ± 0.029
1.498GlnPro: 1.498 ± 0.03
1.911GlnGln: 1.911 ± 0.042
1.802GlnArg: 1.802 ± 0.036
2.454GlnSer: 2.454 ± 0.039
2.128GlnThr: 2.128 ± 0.034
2.454GlnVal: 2.454 ± 0.038
0.562GlnTrp: 0.562 ± 0.016
1.42GlnTyr: 1.42 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
3.184ArgAla: 3.184 ± 0.046
0.351ArgCys: 0.351 ± 0.015
2.417ArgAsp: 2.417 ± 0.033
3.413ArgGlu: 3.413 ± 0.058
2.303ArgPhe: 2.303 ± 0.036
2.843ArgGly: 2.843 ± 0.037
1.032ArgHis: 1.032 ± 0.023
3.757ArgIle: 3.757 ± 0.047
3.081ArgLys: 3.081 ± 0.041
5.154ArgLeu: 5.154 ± 0.069
1.689ArgMet: 1.689 ± 0.032
2.1ArgAsn: 2.1 ± 0.034
1.702ArgPro: 1.702 ± 0.031
1.917ArgGln: 1.917 ± 0.035
2.441ArgArg: 2.441 ± 0.045
3.118ArgSer: 3.118 ± 0.041
2.695ArgThr: 2.695 ± 0.038
2.963ArgVal: 2.963 ± 0.036
0.673ArgTrp: 0.673 ± 0.022
1.887ArgTyr: 1.887 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
4.983SerAla: 4.983 ± 0.052
0.498SerCys: 0.498 ± 0.018
3.277SerAsp: 3.277 ± 0.045
3.907SerGlu: 3.907 ± 0.052
3.143SerPhe: 3.143 ± 0.043
5.544SerGly: 5.544 ± 0.065
1.234SerHis: 1.234 ± 0.026
4.585SerIle: 4.585 ± 0.054
3.346SerLys: 3.346 ± 0.043
6.367SerLeu: 6.367 ± 0.063
1.822SerMet: 1.822 ± 0.03
2.47SerAsn: 2.47 ± 0.042
2.46SerPro: 2.46 ± 0.038
1.932SerGln: 1.932 ± 0.032
3.011SerArg: 3.011 ± 0.045
4.473SerSer: 4.473 ± 0.054
3.134SerThr: 3.134 ± 0.047
4.649SerVal: 4.649 ± 0.085
0.914SerTrp: 0.914 ± 0.025
2.386SerTyr: 2.386 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
4.683ThrAla: 4.683 ± 0.053
0.352ThrCys: 0.352 ± 0.013
2.895ThrAsp: 2.895 ± 0.04
3.349ThrGlu: 3.349 ± 0.048
2.324ThrPhe: 2.324 ± 0.036
4.484ThrGly: 4.484 ± 0.053
0.978ThrHis: 0.978 ± 0.029
4.002ThrIle: 4.002 ± 0.048
2.623ThrLys: 2.623 ± 0.039
5.251ThrLeu: 5.251 ± 0.057
1.336ThrMet: 1.336 ± 0.028
2.13ThrAsn: 2.13 ± 0.039
2.377ThrPro: 2.377 ± 0.047
1.474ThrGln: 1.474 ± 0.03
2.083ThrArg: 2.083 ± 0.035
3.147ThrSer: 3.147 ± 0.038
2.77ThrThr: 2.77 ± 0.043
4.365ThrVal: 4.365 ± 0.049
0.648ThrTrp: 0.648 ± 0.019
1.844ThrTyr: 1.844 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
4.956ValAla: 4.956 ± 0.055
0.618ValCys: 0.618 ± 0.018
3.543ValAsp: 3.543 ± 0.047
4.102ValGlu: 4.102 ± 0.052
2.934ValPhe: 2.934 ± 0.048
4.326ValGly: 4.326 ± 0.066
1.479ValHis: 1.479 ± 0.028
5.124ValIle: 5.124 ± 0.057
3.974ValLys: 3.974 ± 0.054
6.911ValLeu: 6.911 ± 0.075
1.991ValMet: 1.991 ± 0.032
2.938ValAsn: 2.938 ± 0.044
2.944ValPro: 2.944 ± 0.047
2.602ValGln: 2.602 ± 0.038
3.3ValArg: 3.3 ± 0.047
4.883ValSer: 4.883 ± 0.044
4.329ValThr: 4.329 ± 0.054
4.675ValVal: 4.675 ± 0.059
0.924ValTrp: 0.924 ± 0.026
2.41ValTyr: 2.41 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.881TrpAla: 0.881 ± 0.024
0.1TrpCys: 0.1 ± 0.007
0.707TrpAsp: 0.707 ± 0.02
0.838TrpGlu: 0.838 ± 0.019
0.61TrpPhe: 0.61 ± 0.017
0.963TrpGly: 0.963 ± 0.029
0.303TrpHis: 0.303 ± 0.013
0.946TrpIle: 0.946 ± 0.024
0.781TrpLys: 0.781 ± 0.02
1.482TrpLeu: 1.482 ± 0.029
0.451TrpMet: 0.451 ± 0.016
0.754TrpAsn: 0.754 ± 0.018
0.422TrpPro: 0.422 ± 0.015
0.569TrpGln: 0.569 ± 0.019
0.684TrpArg: 0.684 ± 0.018
0.942TrpSer: 0.942 ± 0.024
0.783TrpThr: 0.783 ± 0.022
0.795TrpVal: 0.795 ± 0.022
0.22TrpTrp: 0.22 ± 0.012
0.459TrpTyr: 0.459 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.583TyrAla: 2.583 ± 0.032
0.296TyrCys: 0.296 ± 0.013
1.968TyrAsp: 1.968 ± 0.042
2.303TyrGlu: 2.303 ± 0.036
1.703TyrPhe: 1.703 ± 0.033
2.712TyrGly: 2.712 ± 0.034
0.756TyrHis: 0.756 ± 0.025
2.233TyrIle: 2.233 ± 0.037
1.813TyrLys: 1.813 ± 0.033
3.364TyrLeu: 3.364 ± 0.046
0.972TyrMet: 0.972 ± 0.023
1.541TyrAsn: 1.541 ± 0.032
1.538TyrPro: 1.538 ± 0.027
1.201TyrGln: 1.201 ± 0.026
1.979TyrArg: 1.979 ± 0.034
2.322TyrSer: 2.322 ± 0.038
1.821TyrThr: 1.821 ± 0.033
2.379TyrVal: 2.379 ± 0.033
0.52TyrTrp: 0.52 ± 0.019
1.462TyrTyr: 1.462 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5948 proteins (1929336 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski