Microbotryum silenes-dioicae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Microbotryales; Microbotryaceae; Microbotryum

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X0MI73|A0A2X0MI73_9BASI BQ5605_C009g05751 protein OS=Microbotryum silenes-dioicae OX=796604 GN=BQ5605_C009g05751 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.79SDD4 pKa = 3.65LDD6 pKa = 3.89EE7 pKa = 5.48VALQDD12 pKa = 3.83YY13 pKa = 10.95NDD15 pKa = 4.23YY16 pKa = 10.12EE17 pKa = 4.3HH18 pKa = 7.2SGADD22 pKa = 3.57DD23 pKa = 3.79EE24 pKa = 4.7EE25 pKa = 5.14ARR27 pKa = 11.84AYY29 pKa = 11.12NLIDD33 pKa = 4.27RR34 pKa = 11.84GLATDD39 pKa = 4.7LVVSVCQGFEE49 pKa = 4.0YY50 pKa = 10.16FIVGCFSRR58 pKa = 11.84GVDD61 pKa = 4.76LSWTDD66 pKa = 3.19SRR68 pKa = 5.13

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X0NXA9|A0A2X0NXA9_9BASI BQ5605_C015g07816 protein OS=Microbotryum silenes-dioicae OX=796604 GN=BQ5605_C015g07816 PE=3 SV=1
MM1 pKa = 7.8ASLLRR6 pKa = 11.84NVFSLTSRR14 pKa = 11.84TRR16 pKa = 11.84FTSTRR21 pKa = 11.84SSPSSSTIPTVWRR34 pKa = 11.84SPSTSSISSSTPSAASSSLLSRR56 pKa = 11.84PFSSTPLSRR65 pKa = 11.84GLVRR69 pKa = 11.84KK70 pKa = 6.57PTKK73 pKa = 10.13IKK75 pKa = 10.95LKK77 pKa = 5.73THH79 pKa = 6.31KK80 pKa = 10.25GAAKK84 pKa = 9.84RR85 pKa = 11.84WFAIANGNFKK95 pKa = 10.52RR96 pKa = 11.84SQAGKK101 pKa = 10.3GVLSPTRR108 pKa = 11.84LNRR111 pKa = 11.84LGKK114 pKa = 9.18PAFARR119 pKa = 11.84PIEE122 pKa = 4.29KK123 pKa = 10.26KK124 pKa = 9.95KK125 pKa = 10.6LRR127 pKa = 11.84RR128 pKa = 11.84LMPYY132 pKa = 10.27AA133 pKa = 4.46

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12674

0

12674

6622593

33

6605

522.5

57.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.471 ± 0.021

1.34 ± 0.011

5.656 ± 0.014

5.547 ± 0.018

3.532 ± 0.012

6.627 ± 0.018

2.668 ± 0.01

4.245 ± 0.013

4.333 ± 0.019

9.346 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.007

3.075 ± 0.012

6.473 ± 0.023

3.727 ± 0.014

7.006 ± 0.019

9.006 ± 0.032

6.283 ± 0.015

6.137 ± 0.013

1.376 ± 0.007

2.33 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski