Amino acid dipepetide frequency for Pantoea phage LIMEzero

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.697AlaAla: 15.697 ± 1.778
0.976AlaCys: 0.976 ± 0.304
5.783AlaAsp: 5.783 ± 0.676
5.558AlaGlu: 5.558 ± 0.683
4.431AlaPhe: 4.431 ± 0.672
8.261AlaGly: 8.261 ± 0.918
1.727AlaHis: 1.727 ± 0.394
4.807AlaIle: 4.807 ± 0.489
5.407AlaLys: 5.407 ± 0.867
8.787AlaLeu: 8.787 ± 1.143
3.154AlaMet: 3.154 ± 0.646
4.356AlaAsn: 4.356 ± 0.558
3.154AlaPro: 3.154 ± 0.545
6.083AlaGln: 6.083 ± 1.158
5.257AlaArg: 5.257 ± 0.719
6.91AlaSer: 6.91 ± 1.059
6.684AlaThr: 6.684 ± 0.915
7.811AlaVal: 7.811 ± 0.904
1.202AlaTrp: 1.202 ± 0.336
3.53AlaTyr: 3.53 ± 0.716
0.0AlaXaa: 0.0 ± 0.0
Cys
1.051CysAla: 1.051 ± 0.282
0.0CysCys: 0.0 ± 0.0
0.526CysAsp: 0.526 ± 0.197
0.751CysGlu: 0.751 ± 0.261
0.15CysPhe: 0.15 ± 0.114
0.901CysGly: 0.901 ± 0.291
0.15CysHis: 0.15 ± 0.146
0.751CysIle: 0.751 ± 0.264
0.601CysLys: 0.601 ± 0.238
0.526CysLeu: 0.526 ± 0.189
0.376CysMet: 0.376 ± 0.179
0.526CysAsn: 0.526 ± 0.268
0.526CysPro: 0.526 ± 0.203
0.075CysGln: 0.075 ± 0.089
0.601CysArg: 0.601 ± 0.213
0.676CysSer: 0.676 ± 0.227
0.451CysThr: 0.451 ± 0.242
0.751CysVal: 0.751 ± 0.244
0.225CysTrp: 0.225 ± 0.137
0.376CysTyr: 0.376 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.609AspAla: 6.609 ± 0.703
0.376AspCys: 0.376 ± 0.186
3.38AspAsp: 3.38 ± 0.573
3.605AspGlu: 3.605 ± 0.658
2.178AspPhe: 2.178 ± 0.401
4.882AspGly: 4.882 ± 0.653
0.826AspHis: 0.826 ± 0.26
3.079AspIle: 3.079 ± 0.457
2.178AspLys: 2.178 ± 0.455
5.483AspLeu: 5.483 ± 0.606
2.103AspMet: 2.103 ± 0.515
1.878AspAsn: 1.878 ± 0.376
2.704AspPro: 2.704 ± 0.394
2.328AspGln: 2.328 ± 0.785
2.253AspArg: 2.253 ± 0.404
4.581AspSer: 4.581 ± 0.682
4.281AspThr: 4.281 ± 0.566
4.206AspVal: 4.206 ± 0.674
0.901AspTrp: 0.901 ± 0.308
2.704AspTyr: 2.704 ± 0.56
0.0AspXaa: 0.0 ± 0.0
Glu
5.407GluAla: 5.407 ± 0.741
1.051GluCys: 1.051 ± 0.269
3.38GluAsp: 3.38 ± 0.503
2.478GluGlu: 2.478 ± 0.526
2.403GluPhe: 2.403 ± 0.405
3.83GluGly: 3.83 ± 0.669
1.127GluHis: 1.127 ± 0.322
2.478GluIle: 2.478 ± 0.405
1.727GluLys: 1.727 ± 0.344
5.182GluLeu: 5.182 ± 0.619
1.277GluMet: 1.277 ± 0.31
1.727GluAsn: 1.727 ± 0.331
2.328GluPro: 2.328 ± 0.518
2.478GluGln: 2.478 ± 0.439
3.154GluArg: 3.154 ± 0.474
2.854GluSer: 2.854 ± 0.411
3.305GluThr: 3.305 ± 0.624
3.455GluVal: 3.455 ± 0.562
0.526GluTrp: 0.526 ± 0.189
2.103GluTyr: 2.103 ± 0.395
0.0GluXaa: 0.0 ± 0.0
Phe
3.079PheAla: 3.079 ± 0.413
0.526PheCys: 0.526 ± 0.2
2.554PheAsp: 2.554 ± 0.454
1.202PheGlu: 1.202 ± 0.338
0.751PhePhe: 0.751 ± 0.308
2.328PheGly: 2.328 ± 0.514
0.601PheHis: 0.601 ± 0.211
1.652PheIle: 1.652 ± 0.318
1.427PheLys: 1.427 ± 0.339
1.953PheLeu: 1.953 ± 0.421
1.051PheMet: 1.051 ± 0.304
2.554PheAsn: 2.554 ± 0.52
1.427PhePro: 1.427 ± 0.284
1.577PheGln: 1.577 ± 0.409
1.727PheArg: 1.727 ± 0.31
2.103PheSer: 2.103 ± 0.476
2.478PheThr: 2.478 ± 0.39
3.004PheVal: 3.004 ± 0.402
0.3PheTrp: 0.3 ± 0.156
0.826PheTyr: 0.826 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.759GlyAla: 6.759 ± 0.839
0.451GlyCys: 0.451 ± 0.184
5.032GlyAsp: 5.032 ± 0.481
5.107GlyGlu: 5.107 ± 0.555
2.478GlyPhe: 2.478 ± 0.437
6.534GlyGly: 6.534 ± 1.053
1.127GlyHis: 1.127 ± 0.356
3.605GlyIle: 3.605 ± 0.44
3.68GlyLys: 3.68 ± 0.524
5.633GlyLeu: 5.633 ± 0.706
2.704GlyMet: 2.704 ± 0.379
3.98GlyAsn: 3.98 ± 0.614
1.277GlyPro: 1.277 ± 0.295
5.107GlyGln: 5.107 ± 0.881
4.281GlyArg: 4.281 ± 0.447
4.807GlySer: 4.807 ± 0.778
6.008GlyThr: 6.008 ± 0.875
5.182GlyVal: 5.182 ± 0.6
1.127GlyTrp: 1.127 ± 0.246
3.154GlyTyr: 3.154 ± 0.466
0.0GlyXaa: 0.0 ± 0.0
His
1.802HisAla: 1.802 ± 0.338
0.526HisCys: 0.526 ± 0.153
0.826HisAsp: 0.826 ± 0.24
1.051HisGlu: 1.051 ± 0.267
0.376HisPhe: 0.376 ± 0.155
1.953HisGly: 1.953 ± 0.412
0.376HisHis: 0.376 ± 0.15
0.826HisIle: 0.826 ± 0.25
0.826HisLys: 0.826 ± 0.293
1.652HisLeu: 1.652 ± 0.376
0.526HisMet: 0.526 ± 0.174
0.451HisAsn: 0.451 ± 0.167
0.601HisPro: 0.601 ± 0.268
1.051HisGln: 1.051 ± 0.304
0.901HisArg: 0.901 ± 0.273
0.676HisSer: 0.676 ± 0.195
0.751HisThr: 0.751 ± 0.208
1.502HisVal: 1.502 ± 0.357
0.526HisTrp: 0.526 ± 0.19
0.526HisTyr: 0.526 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
5.107IleAla: 5.107 ± 0.631
0.376IleCys: 0.376 ± 0.148
3.455IleAsp: 3.455 ± 0.526
2.103IleGlu: 2.103 ± 0.382
1.127IlePhe: 1.127 ± 0.293
3.455IleGly: 3.455 ± 0.518
0.751IleHis: 0.751 ± 0.181
3.305IleIle: 3.305 ± 0.549
1.878IleLys: 1.878 ± 0.341
2.779IleLeu: 2.779 ± 0.527
1.202IleMet: 1.202 ± 0.261
2.704IleAsn: 2.704 ± 0.548
2.028IlePro: 2.028 ± 0.294
2.478IleGln: 2.478 ± 0.4
2.403IleArg: 2.403 ± 0.552
3.004IleSer: 3.004 ± 0.459
3.83IleThr: 3.83 ± 0.807
2.779IleVal: 2.779 ± 0.51
0.3IleTrp: 0.3 ± 0.14
1.352IleTyr: 1.352 ± 0.393
0.0IleXaa: 0.0 ± 0.0
Lys
5.933LysAla: 5.933 ± 0.835
0.526LysCys: 0.526 ± 0.196
2.253LysAsp: 2.253 ± 0.541
2.779LysGlu: 2.779 ± 0.471
1.878LysPhe: 1.878 ± 0.343
2.028LysGly: 2.028 ± 0.407
0.751LysHis: 0.751 ± 0.319
1.502LysIle: 1.502 ± 0.359
1.127LysLys: 1.127 ± 0.332
3.905LysLeu: 3.905 ± 0.635
0.826LysMet: 0.826 ± 0.226
1.051LysAsn: 1.051 ± 0.377
1.502LysPro: 1.502 ± 0.271
2.554LysGln: 2.554 ± 0.667
2.253LysArg: 2.253 ± 0.338
3.455LysSer: 3.455 ± 0.486
1.953LysThr: 1.953 ± 0.401
2.779LysVal: 2.779 ± 0.688
0.526LysTrp: 0.526 ± 0.261
0.976LysTyr: 0.976 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
8.712LeuAla: 8.712 ± 0.981
0.751LeuCys: 0.751 ± 0.245
5.483LeuAsp: 5.483 ± 0.6
3.68LeuGlu: 3.68 ± 0.503
2.178LeuPhe: 2.178 ± 0.438
5.107LeuGly: 5.107 ± 0.618
2.253LeuHis: 2.253 ± 0.384
3.38LeuIle: 3.38 ± 0.606
3.004LeuLys: 3.004 ± 0.475
6.834LeuLeu: 6.834 ± 0.598
2.103LeuMet: 2.103 ± 0.45
3.229LeuAsn: 3.229 ± 0.435
3.83LeuPro: 3.83 ± 0.635
3.38LeuGln: 3.38 ± 0.547
4.732LeuArg: 4.732 ± 0.674
5.257LeuSer: 5.257 ± 0.65
6.083LeuThr: 6.083 ± 0.784
5.933LeuVal: 5.933 ± 0.689
0.901LeuTrp: 0.901 ± 0.227
2.779LeuTyr: 2.779 ± 0.411
0.0LeuXaa: 0.0 ± 0.0
Met
3.38MetAla: 3.38 ± 0.576
0.15MetCys: 0.15 ± 0.102
1.427MetAsp: 1.427 ± 0.416
1.427MetGlu: 1.427 ± 0.316
0.901MetPhe: 0.901 ± 0.274
2.103MetGly: 2.103 ± 0.427
0.601MetHis: 0.601 ± 0.203
0.826MetIle: 0.826 ± 0.239
1.502MetLys: 1.502 ± 0.28
2.854MetLeu: 2.854 ± 0.436
1.502MetMet: 1.502 ± 0.457
1.127MetAsn: 1.127 ± 0.257
1.352MetPro: 1.352 ± 0.34
2.103MetGln: 2.103 ± 0.459
2.028MetArg: 2.028 ± 0.466
2.103MetSer: 2.103 ± 0.316
1.953MetThr: 1.953 ± 0.338
1.202MetVal: 1.202 ± 0.395
0.526MetTrp: 0.526 ± 0.251
0.976MetTyr: 0.976 ± 0.28
0.0MetXaa: 0.0 ± 0.0
Asn
4.581AsnAla: 4.581 ± 0.691
0.376AsnCys: 0.376 ± 0.204
1.652AsnAsp: 1.652 ± 0.335
1.652AsnGlu: 1.652 ± 0.319
1.202AsnPhe: 1.202 ± 0.362
3.68AsnGly: 3.68 ± 0.759
0.751AsnHis: 0.751 ± 0.217
1.727AsnIle: 1.727 ± 0.436
1.652AsnLys: 1.652 ± 0.325
4.131AsnLeu: 4.131 ± 0.75
1.277AsnMet: 1.277 ± 0.217
2.629AsnAsn: 2.629 ± 0.547
2.629AsnPro: 2.629 ± 0.683
3.154AsnGln: 3.154 ± 0.575
2.028AsnArg: 2.028 ± 0.327
2.554AsnSer: 2.554 ± 0.48
2.854AsnThr: 2.854 ± 0.66
2.704AsnVal: 2.704 ± 0.399
0.676AsnTrp: 0.676 ± 0.259
1.127AsnTyr: 1.127 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
4.581ProAla: 4.581 ± 0.465
0.225ProCys: 0.225 ± 0.123
3.154ProAsp: 3.154 ± 0.487
3.004ProGlu: 3.004 ± 0.431
1.202ProPhe: 1.202 ± 0.42
4.056ProGly: 4.056 ± 0.575
0.451ProHis: 0.451 ± 0.17
1.727ProIle: 1.727 ± 0.35
1.127ProLys: 1.127 ± 0.259
2.028ProLeu: 2.028 ± 0.445
0.826ProMet: 0.826 ± 0.247
1.277ProAsn: 1.277 ± 0.236
1.277ProPro: 1.277 ± 0.308
3.079ProGln: 3.079 ± 0.899
1.502ProArg: 1.502 ± 0.34
2.478ProSer: 2.478 ± 0.509
3.004ProThr: 3.004 ± 0.531
3.605ProVal: 3.605 ± 0.608
0.676ProTrp: 0.676 ± 0.239
1.802ProTyr: 1.802 ± 0.413
0.0ProXaa: 0.0 ± 0.0
Gln
5.633GlnAla: 5.633 ± 0.879
0.526GlnCys: 0.526 ± 0.183
2.253GlnAsp: 2.253 ± 0.505
2.629GlnGlu: 2.629 ± 0.403
2.704GlnPhe: 2.704 ± 0.684
4.281GlnGly: 4.281 ± 0.735
1.127GlnHis: 1.127 ± 0.296
2.478GlnIle: 2.478 ± 0.539
1.953GlnLys: 1.953 ± 0.535
4.056GlnLeu: 4.056 ± 0.582
2.028GlnMet: 2.028 ± 0.452
1.502GlnAsn: 1.502 ± 0.491
2.328GlnPro: 2.328 ± 0.707
4.882GlnGln: 4.882 ± 2.233
4.131GlnArg: 4.131 ± 0.726
2.704GlnSer: 2.704 ± 0.731
2.779GlnThr: 2.779 ± 0.577
3.905GlnVal: 3.905 ± 0.804
0.826GlnTrp: 0.826 ± 0.222
1.502GlnTyr: 1.502 ± 0.382
0.0GlnXaa: 0.0 ± 0.0
Arg
5.182ArgAla: 5.182 ± 0.884
0.676ArgCys: 0.676 ± 0.224
3.68ArgAsp: 3.68 ± 0.68
2.779ArgGlu: 2.779 ± 0.55
1.427ArgPhe: 1.427 ± 0.303
3.305ArgGly: 3.305 ± 0.501
1.127ArgHis: 1.127 ± 0.317
3.154ArgIle: 3.154 ± 0.461
2.629ArgLys: 2.629 ± 0.553
4.356ArgLeu: 4.356 ± 0.557
2.253ArgMet: 2.253 ± 0.484
1.727ArgAsn: 1.727 ± 0.363
1.878ArgPro: 1.878 ± 0.378
2.328ArgGln: 2.328 ± 0.439
3.305ArgArg: 3.305 ± 0.59
2.854ArgSer: 2.854 ± 0.416
2.403ArgThr: 2.403 ± 0.37
3.53ArgVal: 3.53 ± 0.574
0.901ArgTrp: 0.901 ± 0.302
2.478ArgTyr: 2.478 ± 0.408
0.0ArgXaa: 0.0 ± 0.0
Ser
6.834SerAla: 6.834 ± 0.708
0.075SerCys: 0.075 ± 0.082
3.38SerAsp: 3.38 ± 0.631
3.53SerGlu: 3.53 ± 0.568
2.403SerPhe: 2.403 ± 0.407
6.309SerGly: 6.309 ± 0.525
0.901SerHis: 0.901 ± 0.293
3.229SerIle: 3.229 ± 0.662
2.403SerLys: 2.403 ± 0.331
4.206SerLeu: 4.206 ± 0.502
2.103SerMet: 2.103 ± 0.364
2.779SerAsn: 2.779 ± 0.4
3.079SerPro: 3.079 ± 0.484
2.854SerGln: 2.854 ± 0.429
2.704SerArg: 2.704 ± 0.405
4.581SerSer: 4.581 ± 0.866
5.633SerThr: 5.633 ± 1.159
5.182SerVal: 5.182 ± 0.686
0.901SerTrp: 0.901 ± 0.272
2.028SerTyr: 2.028 ± 0.455
0.0SerXaa: 0.0 ± 0.0
Thr
8.712ThrAla: 8.712 ± 0.994
0.451ThrCys: 0.451 ± 0.2
4.356ThrAsp: 4.356 ± 0.626
2.478ThrGlu: 2.478 ± 0.42
1.953ThrPhe: 1.953 ± 0.406
6.234ThrGly: 6.234 ± 0.926
0.976ThrHis: 0.976 ± 0.249
2.478ThrIle: 2.478 ± 0.486
2.554ThrLys: 2.554 ± 0.391
5.182ThrLeu: 5.182 ± 0.611
1.127ThrMet: 1.127 ± 0.375
3.455ThrAsn: 3.455 ± 0.728
3.154ThrPro: 3.154 ± 0.496
2.929ThrGln: 2.929 ± 0.442
2.478ThrArg: 2.478 ± 0.379
4.807ThrSer: 4.807 ± 0.761
4.882ThrThr: 4.882 ± 0.774
5.633ThrVal: 5.633 ± 1.023
0.526ThrTrp: 0.526 ± 0.189
2.704ThrTyr: 2.704 ± 0.478
0.0ThrXaa: 0.0 ± 0.0
Val
7.06ValAla: 7.06 ± 0.909
0.976ValCys: 0.976 ± 0.263
4.882ValAsp: 4.882 ± 0.751
4.281ValGlu: 4.281 ± 0.503
1.878ValPhe: 1.878 ± 0.295
4.807ValGly: 4.807 ± 0.647
1.277ValHis: 1.277 ± 0.31
2.779ValIle: 2.779 ± 0.445
3.455ValLys: 3.455 ± 0.511
5.708ValLeu: 5.708 ± 0.797
1.953ValMet: 1.953 ± 0.339
3.98ValAsn: 3.98 ± 0.576
4.206ValPro: 4.206 ± 0.671
2.629ValGln: 2.629 ± 0.525
3.305ValArg: 3.305 ± 0.55
4.807ValSer: 4.807 ± 0.695
4.882ValThr: 4.882 ± 0.746
5.708ValVal: 5.708 ± 0.658
0.901ValTrp: 0.901 ± 0.228
2.328ValTyr: 2.328 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
0.826TrpAla: 0.826 ± 0.234
0.526TrpCys: 0.526 ± 0.185
1.202TrpAsp: 1.202 ± 0.247
0.075TrpGlu: 0.075 ± 0.074
0.676TrpPhe: 0.676 ± 0.219
0.601TrpGly: 0.601 ± 0.224
0.225TrpHis: 0.225 ± 0.129
0.3TrpIle: 0.3 ± 0.147
0.376TrpLys: 0.376 ± 0.151
1.577TrpLeu: 1.577 ± 0.392
0.3TrpMet: 0.3 ± 0.125
0.376TrpAsn: 0.376 ± 0.168
0.451TrpPro: 0.451 ± 0.214
1.427TrpGln: 1.427 ± 0.267
0.676TrpArg: 0.676 ± 0.203
1.352TrpSer: 1.352 ± 0.293
0.676TrpThr: 0.676 ± 0.209
0.526TrpVal: 0.526 ± 0.293
0.451TrpTrp: 0.451 ± 0.232
0.376TrpTyr: 0.376 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.629TyrAla: 2.629 ± 0.427
0.451TyrCys: 0.451 ± 0.209
1.878TyrAsp: 1.878 ± 0.289
2.103TyrGlu: 2.103 ± 0.463
0.826TyrPhe: 0.826 ± 0.247
3.38TyrGly: 3.38 ± 0.447
0.601TyrHis: 0.601 ± 0.215
2.403TyrIle: 2.403 ± 0.499
1.051TyrLys: 1.051 ± 0.293
2.779TyrLeu: 2.779 ± 0.512
1.277TyrMet: 1.277 ± 0.286
1.727TyrAsn: 1.727 ± 0.327
1.352TyrPro: 1.352 ± 0.268
1.727TyrGln: 1.727 ± 0.33
2.253TyrArg: 2.253 ± 0.514
2.554TyrSer: 2.554 ± 0.429
2.178TyrThr: 2.178 ± 0.505
2.403TyrVal: 2.403 ± 0.473
0.075TyrTrp: 0.075 ± 0.071
1.202TyrTyr: 1.202 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (13316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski