Amino acid dipepetide frequency for Subdoligranulum sp. APC924/74

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.973AlaAla: 14.973 ± 0.21	1.731AlaCys: 1.731 ± 0.047	6.262AlaAsp: 6.262 ± 0.088	6.819AlaGlu: 6.819 ± 0.09	3.431AlaPhe: 3.431 ± 0.06	7.822AlaGly: 7.822 ± 0.111	1.767AlaHis: 1.767 ± 0.044	4.932AlaIle: 4.932 ± 0.074	4.986AlaLys: 4.986 ± 0.071	10.253AlaLeu: 10.253 ± 0.145	3.041AlaMet: 3.041 ± 0.061	3.135AlaAsn: 3.135 ± 0.055	3.9AlaPro: 3.9 ± 0.086	4.263AlaGln: 4.263 ± 0.073	4.764AlaArg: 4.764 ± 0.083	4.336AlaSer: 4.336 ± 0.067	4.377AlaThr: 4.377 ± 0.078	8.201AlaVal: 8.201 ± 0.109	1.049AlaTrp: 1.049 ± 0.039	2.874AlaTyr: 2.874 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.81CysAla: 1.81 ± 0.05	0.409CysCys: 0.409 ± 0.019	0.961CysAsp: 0.961 ± 0.032	0.892CysGlu: 0.892 ± 0.028	0.649CysPhe: 0.649 ± 0.026	1.88CysGly: 1.88 ± 0.05	0.364CysHis: 0.364 ± 0.021	0.977CysIle: 0.977 ± 0.031	0.864CysLys: 0.864 ± 0.031	1.442CysLeu: 1.442 ± 0.039	0.438CysMet: 0.438 ± 0.021	0.64CysAsn: 0.64 ± 0.024	0.847CysPro: 0.847 ± 0.034	0.498CysGln: 0.498 ± 0.023	1.082CysArg: 1.082 ± 0.034	0.921CysSer: 0.921 ± 0.03	1.03CysThr: 1.03 ± 0.034	1.202CysVal: 1.202 ± 0.038	0.2CysTrp: 0.2 ± 0.014	0.572CysTyr: 0.572 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	6.002AspAla: 6.002 ± 0.081	0.997AspCys: 0.997 ± 0.032	3.158AspAsp: 3.158 ± 0.078	4.052AspGlu: 4.052 ± 0.069	2.499AspPhe: 2.499 ± 0.059	5.008AspGly: 5.008 ± 0.091	0.997AspHis: 0.997 ± 0.028	3.238AspIle: 3.238 ± 0.059	2.756AspLys: 2.756 ± 0.058	5.015AspLeu: 5.015 ± 0.062	1.526AspMet: 1.526 ± 0.037	1.841AspAsn: 1.841 ± 0.049	2.595AspPro: 2.595 ± 0.046	1.371AspGln: 1.371 ± 0.038	2.647AspArg: 2.647 ± 0.056	2.842AspSer: 2.842 ± 0.06	3.527AspThr: 3.527 ± 0.064	3.685AspVal: 3.685 ± 0.068	0.773AspTrp: 0.773 ± 0.027	2.563AspTyr: 2.563 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	5.688GluAla: 5.688 ± 0.085	0.761GluCys: 0.761 ± 0.028	3.123GluAsp: 3.123 ± 0.064	4.29GluGlu: 4.29 ± 0.08	1.983GluPhe: 1.983 ± 0.045	3.444GluGly: 3.444 ± 0.066	1.411GluHis: 1.411 ± 0.038	3.691GluIle: 3.691 ± 0.077	4.043GluLys: 4.043 ± 0.075	6.381GluLeu: 6.381 ± 0.089	1.894GluMet: 1.894 ± 0.044	3.068GluAsn: 3.068 ± 0.059	2.434GluPro: 2.434 ± 0.065	3.107GluGln: 3.107 ± 0.066	3.229GluArg: 3.229 ± 0.067	2.824GluSer: 2.824 ± 0.058	3.497GluThr: 3.497 ± 0.061	3.639GluVal: 3.639 ± 0.079	0.628GluTrp: 0.628 ± 0.029	2.412GluTyr: 2.412 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	4.025PheAla: 4.025 ± 0.077	0.887PheCys: 0.887 ± 0.034	2.675PheAsp: 2.675 ± 0.051	2.035PheGlu: 2.035 ± 0.053	1.618PhePhe: 1.618 ± 0.042	3.13PheGly: 3.13 ± 0.064	0.698PheHis: 0.698 ± 0.027	1.954PheIle: 1.954 ± 0.051	1.526PheLys: 1.526 ± 0.041	3.415PheLeu: 3.415 ± 0.072	0.924PheMet: 0.924 ± 0.029	1.348PheAsn: 1.348 ± 0.037	1.332PhePro: 1.332 ± 0.036	0.955PheGln: 0.955 ± 0.035	1.657PheArg: 1.657 ± 0.043	2.322PheSer: 2.322 ± 0.049	2.468PheThr: 2.468 ± 0.055	2.774PheVal: 2.774 ± 0.054	0.535PheTrp: 0.535 ± 0.027	1.503PheTyr: 1.503 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	6.442GlyAla: 6.442 ± 0.101	1.549GlyCys: 1.549 ± 0.046	3.524GlyAsp: 3.524 ± 0.058	4.168GlyGlu: 4.168 ± 0.066	2.845GlyPhe: 2.845 ± 0.063	5.582GlyGly: 5.582 ± 0.088	1.497GlyHis: 1.497 ± 0.037	4.901GlyIle: 4.901 ± 0.078	4.61GlyLys: 4.61 ± 0.071	6.488GlyLeu: 6.488 ± 0.087	2.388GlyMet: 2.388 ± 0.05	2.664GlyAsn: 2.664 ± 0.062	1.887GlyPro: 1.887 ± 0.049	2.68GlyGln: 2.68 ± 0.061	3.852GlyArg: 3.852 ± 0.067	4.29GlySer: 4.29 ± 0.082	4.802GlyThr: 4.802 ± 0.074	5.531GlyVal: 5.531 ± 0.079	1.013GlyTrp: 1.013 ± 0.035	3.224GlyTyr: 3.224 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.688HisAla: 1.688 ± 0.038	0.424HisCys: 0.424 ± 0.023	1.09HisAsp: 1.09 ± 0.037	1.086HisGlu: 1.086 ± 0.03	0.891HisPhe: 0.891 ± 0.032	1.519HisGly: 1.519 ± 0.04	0.494HisHis: 0.494 ± 0.033	1.185HisIle: 1.185 ± 0.039	0.873HisLys: 0.873 ± 0.036	1.769HisLeu: 1.769 ± 0.046	0.525HisMet: 0.525 ± 0.021	0.775HisAsn: 0.775 ± 0.028	1.144HisPro: 1.144 ± 0.038	0.549HisGln: 0.549 ± 0.025	1.052HisArg: 1.052 ± 0.03	1.052HisSer: 1.052 ± 0.035	1.297HisThr: 1.297 ± 0.041	1.135HisVal: 1.135 ± 0.038	0.275HisTrp: 0.275 ± 0.017	0.851HisTyr: 0.851 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.444IleAla: 5.444 ± 0.071	1.081IleCys: 1.081 ± 0.03	3.208IleAsp: 3.208 ± 0.06	3.208IleGlu: 3.208 ± 0.066	2.087IlePhe: 2.087 ± 0.05	3.962IleGly: 3.962 ± 0.078	1.03IleHis: 1.03 ± 0.034	2.97IleIle: 2.97 ± 0.067	2.523IleLys: 2.523 ± 0.055	5.29IleLeu: 5.29 ± 0.082	1.328IleMet: 1.328 ± 0.039	2.11IleAsn: 2.11 ± 0.052	2.682IlePro: 2.682 ± 0.046	1.701IleGln: 1.701 ± 0.04	3.002IleArg: 3.002 ± 0.06	3.36IleSer: 3.36 ± 0.066	3.552IleThr: 3.552 ± 0.057	3.847IleVal: 3.847 ± 0.07	0.601IleTrp: 0.601 ± 0.025	1.945IleTyr: 1.945 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	5.048LysAla: 5.048 ± 0.083	0.556LysCys: 0.556 ± 0.025	2.791LysAsp: 2.791 ± 0.062	3.569LysGlu: 3.569 ± 0.081	1.489LysPhe: 1.489 ± 0.037	3.182LysGly: 3.182 ± 0.06	0.884LysHis: 0.884 ± 0.033	2.836LysIle: 2.836 ± 0.055	3.501LysLys: 3.501 ± 0.076	4.459LysLeu: 4.459 ± 0.079	1.562LysMet: 1.562 ± 0.043	2.309LysAsn: 2.309 ± 0.056	2.199LysPro: 2.199 ± 0.05	2.236LysGln: 2.236 ± 0.05	2.57LysArg: 2.57 ± 0.058	2.51LysSer: 2.51 ± 0.055	3.201LysThr: 3.201 ± 0.056	3.188LysVal: 3.188 ± 0.068	0.506LysTrp: 0.506 ± 0.022	1.959LysTyr: 1.959 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	9.9LeuAla: 9.9 ± 0.143	2.272LeuCys: 2.272 ± 0.054	5.732LeuAsp: 5.732 ± 0.079	5.292LeuGlu: 5.292 ± 0.076	3.736LeuPhe: 3.736 ± 0.077	6.421LeuGly: 6.421 ± 0.102	2.116LeuHis: 2.116 ± 0.049	4.591LeuIle: 4.591 ± 0.074	4.21LeuLys: 4.21 ± 0.071	9.725LeuLeu: 9.725 ± 0.151	2.464LeuMet: 2.464 ± 0.052	3.593LeuAsn: 3.593 ± 0.072	5.128LeuPro: 5.128 ± 0.073	3.7LeuGln: 3.7 ± 0.058	5.277LeuArg: 5.277 ± 0.084	5.215LeuSer: 5.215 ± 0.081	5.969LeuThr: 5.969 ± 0.071	6.139LeuVal: 6.139 ± 0.091	1.062LeuTrp: 1.062 ± 0.038	3.422LeuTyr: 3.422 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.882MetAla: 2.882 ± 0.055	0.336MetCys: 0.336 ± 0.018	1.652MetAsp: 1.652 ± 0.038	1.738MetGlu: 1.738 ± 0.046	0.881MetPhe: 0.881 ± 0.029	1.892MetGly: 1.892 ± 0.05	0.464MetHis: 0.464 ± 0.024	1.501MetIle: 1.501 ± 0.044	1.698MetLys: 1.698 ± 0.044	2.755MetLeu: 2.755 ± 0.056	0.831MetMet: 0.831 ± 0.032	1.21MetAsn: 1.21 ± 0.039	1.504MetPro: 1.504 ± 0.036	1.395MetGln: 1.395 ± 0.039	1.393MetArg: 1.393 ± 0.039	1.416MetSer: 1.416 ± 0.038	1.732MetThr: 1.732 ± 0.043	1.817MetVal: 1.817 ± 0.041	0.254MetTrp: 0.254 ± 0.017	0.745MetTyr: 0.745 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.725AsnAla: 3.725 ± 0.073	0.606AsnCys: 0.606 ± 0.025	1.928AsnAsp: 1.928 ± 0.045	2.025AsnGlu: 2.025 ± 0.052	1.449AsnPhe: 1.449 ± 0.044	3.409AsnGly: 3.409 ± 0.065	0.768AsnHis: 0.768 ± 0.034	2.333AsnIle: 2.333 ± 0.057	1.667AsnLys: 1.667 ± 0.044	3.391AsnLeu: 3.391 ± 0.061	1.031AsnMet: 1.031 ± 0.031	1.398AsnAsn: 1.398 ± 0.047	1.958AsnPro: 1.958 ± 0.046	1.22AsnGln: 1.22 ± 0.037	1.965AsnArg: 1.965 ± 0.05	1.884AsnSer: 1.884 ± 0.054	2.311AsnThr: 2.311 ± 0.055	2.475AsnVal: 2.475 ± 0.053	0.437AsnTrp: 0.437 ± 0.022	1.541AsnTyr: 1.541 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	4.704ProAla: 4.704 ± 0.082	0.681ProCys: 0.681 ± 0.031	3.196ProAsp: 3.196 ± 0.064	3.8ProGlu: 3.8 ± 0.067	1.697ProPhe: 1.697 ± 0.043	3.236ProGly: 3.236 ± 0.063	0.854ProHis: 0.854 ± 0.031	1.999ProIle: 1.999 ± 0.048	1.945ProLys: 1.945 ± 0.05	3.768ProLeu: 3.768 ± 0.067	1.011ProMet: 1.011 ± 0.032	1.488ProAsn: 1.488 ± 0.05	1.382ProPro: 1.382 ± 0.053	2.04ProGln: 2.04 ± 0.054	1.752ProArg: 1.752 ± 0.044	1.908ProSer: 1.908 ± 0.043	2.279ProThr: 2.279 ± 0.066	3.489ProVal: 3.489 ± 0.062	0.451ProTrp: 0.451 ± 0.025	1.678ProTyr: 1.678 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.89GlnAla: 3.89 ± 0.081	0.446GlnCys: 0.446 ± 0.022	1.954GlnAsp: 1.954 ± 0.044	2.292GlnGlu: 2.292 ± 0.055	1.266GlnPhe: 1.266 ± 0.033	2.419GlnGly: 2.419 ± 0.052	0.723GlnHis: 0.723 ± 0.025	2.08GlnIle: 2.08 ± 0.051	2.372GlnLys: 2.372 ± 0.04	3.669GlnLeu: 3.669 ± 0.069	1.172GlnMet: 1.172 ± 0.037	1.827GlnAsn: 1.827 ± 0.045	1.786GlnPro: 1.786 ± 0.051	2.056GlnGln: 2.056 ± 0.061	2.21GlnArg: 2.21 ± 0.055	1.979GlnSer: 1.979 ± 0.047	2.211GlnThr: 2.211 ± 0.053	2.215GlnVal: 2.215 ± 0.049	0.44GlnTrp: 0.44 ± 0.023	1.48GlnTyr: 1.48 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	4.557ArgAla: 4.557 ± 0.073	0.935ArgCys: 0.935 ± 0.032	2.71ArgAsp: 2.71 ± 0.05	3.285ArgGlu: 3.285 ± 0.062	2.01ArgPhe: 2.01 ± 0.044	3.23ArgGly: 3.23 ± 0.063	1.114ArgHis: 1.114 ± 0.027	3.05ArgIle: 3.05 ± 0.059	2.92ArgLys: 2.92 ± 0.056	5.032ArgLeu: 5.032 ± 0.076	1.709ArgMet: 1.709 ± 0.042	1.823ArgAsn: 1.823 ± 0.047	2.099ArgPro: 2.099 ± 0.046	2.244ArgGln: 2.244 ± 0.051	3.337ArgArg: 3.337 ± 0.07	2.577ArgSer: 2.577 ± 0.059	2.959ArgThr: 2.959 ± 0.06	3.195ArgVal: 3.195 ± 0.061	0.696ArgTrp: 0.696 ± 0.028	2.159ArgTyr: 2.159 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.067SerAla: 5.067 ± 0.078	0.837SerCys: 0.837 ± 0.03	2.896SerAsp: 2.896 ± 0.056	2.882SerGlu: 2.882 ± 0.047	2.264SerPhe: 2.264 ± 0.055	4.611SerGly: 4.611 ± 0.072	0.992SerHis: 0.992 ± 0.028	3.056SerIle: 3.056 ± 0.062	2.315SerLys: 2.315 ± 0.049	4.653SerLeu: 4.653 ± 0.073	1.515SerMet: 1.515 ± 0.042	1.746SerAsn: 1.746 ± 0.047	1.94SerPro: 1.94 ± 0.049	1.635SerGln: 1.635 ± 0.037	2.695SerArg: 2.695 ± 0.05	2.998SerSer: 2.998 ± 0.074	3.157SerThr: 3.157 ± 0.074	3.728SerVal: 3.728 ± 0.067	0.601SerTrp: 0.601 ± 0.024	2.027SerTyr: 2.027 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	6.806ThrAla: 6.806 ± 0.103	0.827ThrCys: 0.827 ± 0.029	3.26ThrAsp: 3.26 ± 0.062	3.351ThrGlu: 3.351 ± 0.068	2.085ThrPhe: 2.085 ± 0.048	4.962ThrGly: 4.962 ± 0.09	1.08ThrHis: 1.08 ± 0.034	3.36ThrIle: 3.36 ± 0.061	2.357ThrLys: 2.357 ± 0.054	6.004ThrLeu: 6.004 ± 0.084	1.573ThrMet: 1.573 ± 0.038	1.89ThrAsn: 1.89 ± 0.047	3.355ThrPro: 3.355 ± 0.069	1.867ThrGln: 1.867 ± 0.049	2.709ThrArg: 2.709 ± 0.051	2.745ThrSer: 2.745 ± 0.055	3.23ThrThr: 3.23 ± 0.07	5.07ThrVal: 5.07 ± 0.071	0.637ThrTrp: 0.637 ± 0.027	1.942ThrTyr: 1.942 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	5.869ValAla: 5.869 ± 0.089	1.387ValCys: 1.387 ± 0.036	3.906ValAsp: 3.906 ± 0.064	4.025ValGlu: 4.025 ± 0.067	2.85ValPhe: 2.85 ± 0.061	4.506ValGly: 4.506 ± 0.082	1.242ValHis: 1.242 ± 0.036	3.778ValIle: 3.778 ± 0.071	3.222ValLys: 3.222 ± 0.064	7.505ValLeu: 7.505 ± 0.115	1.929ValMet: 1.929 ± 0.046	2.521ValAsn: 2.521 ± 0.055	3.259ValPro: 3.259 ± 0.07	2.87ValGln: 2.87 ± 0.057	3.661ValArg: 3.661 ± 0.063	3.927ValSer: 3.927 ± 0.063	4.465ValThr: 4.465 ± 0.076	4.824ValVal: 4.824 ± 0.082	0.816ValTrp: 0.816 ± 0.027	2.468ValTyr: 2.468 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.969TrpAla: 0.969 ± 0.03	0.27TrpCys: 0.27 ± 0.018	0.636TrpAsp: 0.636 ± 0.028	0.629TrpGlu: 0.629 ± 0.026	0.498TrpPhe: 0.498 ± 0.022	0.81TrpGly: 0.81 ± 0.031	0.307TrpHis: 0.307 ± 0.017	0.467TrpIle: 0.467 ± 0.022	0.573TrpLys: 0.573 ± 0.023	1.283TrpLeu: 1.283 ± 0.046	0.363TrpMet: 0.363 ± 0.022	0.577TrpAsn: 0.577 ± 0.027	0.423TrpPro: 0.423 ± 0.019	0.775TrpGln: 0.775 ± 0.031	0.605TrpArg: 0.605 ± 0.028	0.507TrpSer: 0.507 ± 0.022	0.499TrpThr: 0.499 ± 0.024	0.739TrpVal: 0.739 ± 0.03	0.154TrpTrp: 0.154 ± 0.013	0.513TrpTyr: 0.513 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.425TyrAla: 3.425 ± 0.054	0.688TyrCys: 0.688 ± 0.025	2.429TyrAsp: 2.429 ± 0.063	2.111TyrGlu: 2.111 ± 0.054	1.552TyrPhe: 1.552 ± 0.046	2.916TyrGly: 2.916 ± 0.057	0.872TyrHis: 0.872 ± 0.033	2.032TyrIle: 2.032 ± 0.052	1.592TyrLys: 1.592 ± 0.042	3.67TyrLeu: 3.67 ± 0.065	0.87TyrMet: 0.87 ± 0.03	1.559TyrAsn: 1.559 ± 0.044	1.501TyrPro: 1.501 ± 0.041	1.323TyrGln: 1.323 ± 0.037	2.186TyrArg: 2.186 ± 0.045	2.003TyrSer: 2.003 ± 0.043	2.545TyrThr: 2.545 ± 0.057	2.136TyrVal: 2.136 ± 0.046	0.47TyrTrp: 0.47 ± 0.025	1.591TyrTyr: 1.591 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3080 proteins (977135 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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