Simiduia agarivorans (strain DSM 21679 / JCM 13881 / BCRC 17597 / SA1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Simiduia; Simiduia agarivorans

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4KKL7|K4KKL7_SIMAS rRNA methylase OS=Simiduia agarivorans (strain DSM 21679 / JCM 13881 / BCRC 17597 / SA1) OX=1117647 GN=M5M_12565 PE=4 SV=1
MM1 pKa = 7.35SGWNTSVAGKK11 pKa = 10.16LLLSTSISLLVACGGGGGGAPAPTPTPAPTPAPSPTPTPTPTPTPEE57 pKa = 4.05PTPTPTPTPTPTPEE71 pKa = 3.98PTPTPEE77 pKa = 3.98PTPTPEE83 pKa = 4.08PTPTPTPTPTPTPEE97 pKa = 4.05PTPTPTPTPTPTPTGFAVSGSVSGLNNGYY126 pKa = 7.93YY127 pKa = 10.22TLTLNGTEE135 pKa = 4.3SVTVEE140 pKa = 3.98ANASSFSFQTLLSTDD155 pKa = 2.78ASYY158 pKa = 11.06QVVASGEE165 pKa = 4.16NSTQFCTLAGGEE177 pKa = 4.39GVIGAANVSNLSLSCFGANGYY198 pKa = 6.79TLQGYY203 pKa = 7.72GLQTQSPSIVVMGLRR218 pKa = 11.84VTDD221 pKa = 4.39LASGSPAPEE230 pKa = 3.72NSFSDD235 pKa = 3.14SHH237 pKa = 6.84FVVKK241 pKa = 10.68EE242 pKa = 3.87NGSSVGSEE250 pKa = 3.98SFISAEE256 pKa = 4.01PVANAALDD264 pKa = 3.75LRR266 pKa = 11.84TVIVLDD272 pKa = 3.99ISQSLTPTDD281 pKa = 3.58VEE283 pKa = 4.74DD284 pKa = 3.57IKK286 pKa = 11.44VAAKK290 pKa = 9.99QAIFTTNGDD299 pKa = 3.29GSKK302 pKa = 9.67TSNLVPGQRR311 pKa = 11.84VAIYY315 pKa = 9.64TFDD318 pKa = 3.6STVKK322 pKa = 10.33LVSDD326 pKa = 4.52FSDD329 pKa = 5.47DD330 pKa = 3.17ITALDD335 pKa = 3.72QQIDD339 pKa = 4.3SIDD342 pKa = 3.46SSLVNRR348 pKa = 11.84GNSTNLYY355 pKa = 10.21GAIQTAVGRR364 pKa = 11.84WTNSFTLTSAQFGYY378 pKa = 9.47TIVITDD384 pKa = 3.69GDD386 pKa = 4.23HH387 pKa = 6.59NADD390 pKa = 3.3SRR392 pKa = 11.84TAADD396 pKa = 3.64ISASTTGKK404 pKa = 10.23DD405 pKa = 2.72IYY407 pKa = 10.84AIAVGGDD414 pKa = 3.17VTLANLEE421 pKa = 4.27QVTGSADD428 pKa = 3.45RR429 pKa = 11.84VFTVADD435 pKa = 3.55IGALNAQLDD444 pKa = 4.79EE445 pKa = 4.34IQQAAIDD452 pKa = 3.97QTKK455 pKa = 9.13GLYY458 pKa = 9.45RR459 pKa = 11.84VFYY462 pKa = 8.26ATPKK466 pKa = 9.97RR467 pKa = 11.84SGTHH471 pKa = 5.14EE472 pKa = 4.63VILSLSPEE480 pKa = 4.31YY481 pKa = 10.72TCDD484 pKa = 3.5AQISGCITGLSGTFNSNGFTDD505 pKa = 5.23VIPEE509 pKa = 4.25IYY511 pKa = 10.23VSHH514 pKa = 7.71DD515 pKa = 2.93KK516 pKa = 11.29GKK518 pKa = 10.38EE519 pKa = 3.68VSTNNWAVTPDD530 pKa = 3.23TDD532 pKa = 3.45ITLTSKK538 pKa = 10.64LRR540 pKa = 11.84WANITPAFGYY550 pKa = 9.67SWSNTSGAEE559 pKa = 3.85ATLTAIEE566 pKa = 4.38GTNQQVLNVGLDD578 pKa = 4.01FVQSDD583 pKa = 4.12LLIADD588 pKa = 4.0SASGLSRR595 pKa = 11.84TITYY599 pKa = 10.41HH600 pKa = 7.34LDD602 pKa = 3.23TDD604 pKa = 3.93GDD606 pKa = 4.55GIIDD610 pKa = 3.83TTDD613 pKa = 3.05TDD615 pKa = 4.06DD616 pKa = 6.11DD617 pKa = 4.52NDD619 pKa = 3.95GVLDD623 pKa = 3.67VDD625 pKa = 4.86DD626 pKa = 5.9EE627 pKa = 4.34MRR629 pKa = 11.84QDD631 pKa = 3.43PSEE634 pKa = 4.08TLDD637 pKa = 3.47TDD639 pKa = 3.86GDD641 pKa = 4.61GIGNNADD648 pKa = 3.63TDD650 pKa = 4.11DD651 pKa = 5.5DD652 pKa = 5.15GDD654 pKa = 4.09GVADD658 pKa = 3.83VDD660 pKa = 5.33DD661 pKa = 5.88AFPLDD666 pKa = 3.82NQEE669 pKa = 5.11SLDD672 pKa = 3.78TDD674 pKa = 3.68GDD676 pKa = 4.57GIGNNADD683 pKa = 4.0PDD685 pKa = 4.17DD686 pKa = 5.9DD687 pKa = 4.52NDD689 pKa = 4.0GVADD693 pKa = 4.24EE694 pKa = 5.23SDD696 pKa = 4.22AFPLDD701 pKa = 3.43ASEE704 pKa = 4.35TTDD707 pKa = 3.24TDD709 pKa = 3.54GDD711 pKa = 4.53GIGDD715 pKa = 3.68NADD718 pKa = 3.48PDD720 pKa = 4.11KK721 pKa = 11.09DD722 pKa = 3.9VPSAMGEE729 pKa = 4.35SYY731 pKa = 10.24TLDD734 pKa = 3.37TFAVTPAGALLNVLSNDD751 pKa = 3.24TFGLDD756 pKa = 3.62GAGSLSIAVQPSHH769 pKa = 6.79GSVSLDD775 pKa = 3.75DD776 pKa = 4.44NGTPEE781 pKa = 5.27DD782 pKa = 4.3ASDD785 pKa = 3.58DD786 pKa = 3.65QLRR789 pKa = 11.84FIPADD794 pKa = 3.49GYY796 pKa = 10.39MGSDD800 pKa = 2.73QFTYY804 pKa = 10.53EE805 pKa = 3.92IADD808 pKa = 3.74GSGSTTQALVDD819 pKa = 3.82LTIGGIRR826 pKa = 11.84SLTVSASEE834 pKa = 3.57KK835 pKa = 9.75HH836 pKa = 4.92GQIRR840 pKa = 11.84VSWDD844 pKa = 3.24AQPGANVSHH853 pKa = 5.94YY854 pKa = 9.16TLLVNPDD861 pKa = 3.32GASGYY866 pKa = 8.67TPVAGAEE873 pKa = 4.04MLLVDD878 pKa = 4.51TIYY881 pKa = 11.32YY882 pKa = 10.03DD883 pKa = 3.58IEE885 pKa = 4.59VSVLNYY891 pKa = 10.93DD892 pKa = 4.99FINASYY898 pKa = 9.82MLEE901 pKa = 4.07LRR903 pKa = 11.84DD904 pKa = 3.48SSEE907 pKa = 3.98NVLEE911 pKa = 5.1DD912 pKa = 3.82GLVLVNGLSSSDD924 pKa = 3.01LVTYY928 pKa = 10.63IKK930 pKa = 10.75ASNTDD935 pKa = 2.79AGDD938 pKa = 3.51RR939 pKa = 11.84FGSSVSVSNDD949 pKa = 2.6GTVIAVGAKK958 pKa = 10.29FEE960 pKa = 4.68DD961 pKa = 3.95SAGNGVYY968 pKa = 10.97GNISAGNSSFTDD980 pKa = 3.05SGAVYY985 pKa = 10.35VYY987 pKa = 11.15SKK989 pKa = 11.48LNGVWQQQAYY999 pKa = 9.17IKK1001 pKa = 10.86APLASGASDD1010 pKa = 3.35FFGYY1014 pKa = 9.97SVSLSGDD1021 pKa = 3.11GSTLAVGAYY1030 pKa = 10.3GEE1032 pKa = 4.58DD1033 pKa = 3.88SEE1035 pKa = 5.14LTGSNAPYY1043 pKa = 10.72NNTGTDD1049 pKa = 2.93SGAVYY1054 pKa = 10.51IFTQNAGVWASQAYY1068 pKa = 10.07LKK1070 pKa = 10.36AQNIGHH1076 pKa = 6.48YY1077 pKa = 9.68DD1078 pKa = 3.37YY1079 pKa = 11.39FGFSVSLSTDD1089 pKa = 3.24GNVLAVGAYY1098 pKa = 10.1NEE1100 pKa = 4.86GGSATGVNGVVDD1112 pKa = 3.93NLAAGSGAAYY1122 pKa = 10.22VFNRR1126 pKa = 11.84DD1127 pKa = 3.49GINWSQSAYY1136 pKa = 10.37VKK1138 pKa = 10.81ASNTDD1143 pKa = 3.08ASDD1146 pKa = 3.21MFGFSVGLSGDD1157 pKa = 3.47GNTLVVGAPGEE1168 pKa = 4.68DD1169 pKa = 3.76GASTGIYY1176 pKa = 10.41SVDD1179 pKa = 3.13QSSNGKK1185 pKa = 8.97SSSGAVYY1192 pKa = 9.82VFNYY1196 pKa = 10.05EE1197 pKa = 3.8GTEE1200 pKa = 4.17WVQDD1204 pKa = 3.77SYY1206 pKa = 12.22VKK1208 pKa = 10.95ANNTDD1213 pKa = 3.17SDD1215 pKa = 3.96DD1216 pKa = 3.63QFGYY1220 pKa = 10.04SVSVSADD1227 pKa = 2.88GRR1229 pKa = 11.84DD1230 pKa = 3.64FAVGAIGEE1238 pKa = 4.4DD1239 pKa = 3.12WSGQNPINNNVSNSGAVFMFKK1260 pKa = 9.34KK1261 pKa = 6.8TTYY1264 pKa = 8.93WYY1266 pKa = 10.44QWDD1269 pKa = 3.9HH1270 pKa = 6.46LKK1272 pKa = 11.05ASTPAAKK1279 pKa = 10.43DD1280 pKa = 3.17NFGFSVSISPDD1291 pKa = 2.92GKK1293 pKa = 9.58SVVVGSVFEE1302 pKa = 4.36ASNSVGINPIDD1313 pKa = 3.61EE1314 pKa = 4.72NNFNAQRR1321 pKa = 11.84GAAYY1325 pKa = 10.0LFRR1328 pKa = 11.84SSEE1331 pKa = 4.23PNDD1334 pKa = 3.12HH1335 pKa = 7.51WIQTHH1340 pKa = 5.75YY1341 pKa = 10.41IKK1343 pKa = 10.86SPHH1346 pKa = 5.93TGSNITLGRR1355 pKa = 11.84SVAVSDD1361 pKa = 3.72NANTIVIGADD1371 pKa = 3.66GEE1373 pKa = 4.28NSAATGINSTPTGSKK1388 pKa = 7.6TASGAVYY1395 pKa = 10.11VYY1397 pKa = 10.76RR1398 pKa = 5.83

Molecular weight: 144.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4L0S2|K4L0S2_SIMAS Probable membrane transporter protein OS=Simiduia agarivorans (strain DSM 21679 / JCM 13881 / BCRC 17597 / SA1) OX=1117647 GN=M5M_12985 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 9.74RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.25GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 7.34QLSVAA45 pKa = 3.87

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3812

0

3812

1294979

29

4336

339.7

37.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.91 ± 0.053

1.035 ± 0.013

5.784 ± 0.027

5.656 ± 0.044

3.815 ± 0.026

7.589 ± 0.052

2.27 ± 0.016

5.004 ± 0.031

3.848 ± 0.036

10.848 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.02

3.636 ± 0.036

4.55 ± 0.03

4.627 ± 0.03

5.602 ± 0.039

5.914 ± 0.038

5.2 ± 0.038

7.028 ± 0.035

1.488 ± 0.019

2.828 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski