Reichenbachiella sp. 5M10

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Reichenbachiellaceae; Reichenbachiella; unclassified Reichenbachiella

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5L0P1|A0A2G5L0P1_9BACT Acyl-CoA oxidase OS=Reichenbachiella sp. 5M10 OX=1889772 GN=BFP72_03775 PE=3 SV=1
MM1 pKa = 7.39LAILSMVSVSYY12 pKa = 10.54AQNQAPVFTSTPVVTVDD29 pKa = 3.97DD30 pKa = 4.22NVAYY34 pKa = 10.43SYY36 pKa = 9.65TAYY39 pKa = 9.86TSDD42 pKa = 3.25SDD44 pKa = 3.65RR45 pKa = 11.84DD46 pKa = 3.81RR47 pKa = 11.84VTVTATTKK55 pKa = 10.51PSWLTLTNIDD65 pKa = 4.48AEE67 pKa = 4.61VTTFAGSGSSGSEE80 pKa = 3.97DD81 pKa = 3.2GHH83 pKa = 5.63GTSASFNNPSGIAIDD98 pKa = 3.7ASGNIYY104 pKa = 9.55VANANTIRR112 pKa = 11.84KK113 pKa = 8.78ISPSGDD119 pKa = 3.14VTTLAGSAVAGDD131 pKa = 3.6TDD133 pKa = 4.36GNGVNATFNLPKK145 pKa = 10.62GLAIDD150 pKa = 3.68AAGNVYY156 pKa = 10.46VADD159 pKa = 3.96TYY161 pKa = 11.13SFKK164 pKa = 10.32IRR166 pKa = 11.84KK167 pKa = 8.89ISTSGDD173 pKa = 3.21VTTLAGSGNVASIDD187 pKa = 3.72GNGANASFNYY197 pKa = 9.47PFALAVDD204 pKa = 3.93ATGNVYY210 pKa = 10.47VADD213 pKa = 4.47GGSQKK218 pKa = 9.59IRR220 pKa = 11.84KK221 pKa = 6.57ITPDD225 pKa = 3.44GEE227 pKa = 4.43VTTFAGSGDD236 pKa = 3.75NEE238 pKa = 4.14NVDD241 pKa = 4.2GNGLNASFISPRR253 pKa = 11.84GLTIDD258 pKa = 3.38AAGNLYY264 pKa = 8.33VTSFYY269 pKa = 11.13GHH271 pKa = 7.38AVRR274 pKa = 11.84KK275 pKa = 7.52ITPDD279 pKa = 3.34RR280 pKa = 11.84DD281 pKa = 3.44VTTLAGSGTAGNTDD295 pKa = 3.92DD296 pKa = 5.04NGTSASFNEE305 pKa = 4.21PAGIAVDD312 pKa = 4.24GSGNLYY318 pKa = 10.4VADD321 pKa = 3.88NRR323 pKa = 11.84NHH325 pKa = 7.48KK326 pKa = 9.21IRR328 pKa = 11.84KK329 pKa = 8.51ISTDD333 pKa = 3.06GDD335 pKa = 3.79VTTLAGSGNPGSTDD349 pKa = 3.28EE350 pKa = 4.88NGTSASFNNPIGVAVDD366 pKa = 3.49AAGSVYY372 pKa = 10.76VADD375 pKa = 3.96QVNHH379 pKa = 7.53KK380 pKa = 9.36IRR382 pKa = 11.84KK383 pKa = 6.73ISKK386 pKa = 7.95YY387 pKa = 10.13YY388 pKa = 9.72EE389 pKa = 4.06LSGSATDD396 pKa = 3.93QLGDD400 pKa = 3.75HH401 pKa = 6.48NVTLTASDD409 pKa = 3.96GKK411 pKa = 11.06GGTATQSFVLKK422 pKa = 9.57VAKK425 pKa = 10.17GRR427 pKa = 11.84AIGTGNILYY436 pKa = 10.03VDD438 pKa = 4.12KK439 pKa = 10.98NVSGGDD445 pKa = 3.46EE446 pKa = 4.59RR447 pKa = 11.84GSSWINAVPEE457 pKa = 4.08LADD460 pKa = 3.52ALVWAKK466 pKa = 11.15DD467 pKa = 3.58NYY469 pKa = 10.87DD470 pKa = 3.95DD471 pKa = 3.79TWATTPLKK479 pKa = 10.43IYY481 pKa = 8.86VAKK484 pKa = 9.34GTYY487 pKa = 9.09KK488 pKa = 10.6PLYY491 pKa = 10.01SPEE494 pKa = 5.55DD495 pKa = 3.67GDD497 pKa = 5.65NFGTDD502 pKa = 2.92QGRR505 pKa = 11.84NNSFSMVKK513 pKa = 10.08NVQLYY518 pKa = 11.07GGFDD522 pKa = 3.62PANGIEE528 pKa = 4.69DD529 pKa = 3.8LTDD532 pKa = 3.36TRR534 pKa = 11.84ILPSSDD540 pKa = 3.05EE541 pKa = 4.14AVGTILSGDD550 pKa = 3.27IGTADD555 pKa = 3.95DD556 pKa = 4.43ATDD559 pKa = 3.07NTYY562 pKa = 11.28NVLVSAGDD570 pKa = 3.42VGTASLDD577 pKa = 3.54GFSITDD583 pKa = 4.47GYY585 pKa = 11.06ANDD588 pKa = 3.69YY589 pKa = 10.91SDD591 pKa = 4.24IYY593 pKa = 11.72VNGKK597 pKa = 9.55QIFKK601 pKa = 10.56CSGAGVYY608 pKa = 10.6NSVSSPTYY616 pKa = 10.35KK617 pKa = 10.42HH618 pKa = 5.12VTLHH622 pKa = 6.27NNSCTEE628 pKa = 3.79SGGGMFSYY636 pKa = 10.05MSSPALQLVILKK648 pKa = 10.26NNQAKK653 pKa = 10.66DD654 pKa = 3.29GGGITNQEE662 pKa = 4.14SSSPTLLNVSIQSNNASEE680 pKa = 4.65NGSGMYY686 pKa = 10.25NVDD689 pKa = 3.21EE690 pKa = 4.84SEE692 pKa = 4.06PTLTNVSIVGNEE704 pKa = 3.62ASVSSEE710 pKa = 3.89EE711 pKa = 4.21VPSCVSQNSSLTLNNCIIWDD731 pKa = 4.22VVTGDD736 pKa = 3.48YY737 pKa = 8.23TAQNSLIKK745 pKa = 9.99GTSDD749 pKa = 3.07TSNGNLDD756 pKa = 3.63ATDD759 pKa = 4.07LTDD762 pKa = 3.36TDD764 pKa = 4.26IFTDD768 pKa = 4.19PANGDD773 pKa = 3.55YY774 pKa = 10.7SLKK777 pKa = 9.21STSIAVNSGSNTLYY791 pKa = 10.45TDD793 pKa = 3.87AGGDD797 pKa = 3.59LANDD801 pKa = 3.05VDD803 pKa = 3.74IAGNARR809 pKa = 11.84LYY811 pKa = 10.99DD812 pKa = 4.81GIPSTDD818 pKa = 3.69IIDD821 pKa = 3.66MGAYY825 pKa = 9.35EE826 pKa = 4.64YY827 pKa = 10.98LDD829 pKa = 4.51NIDD832 pKa = 4.29PVFTSTTTASFIEE845 pKa = 4.53NEE847 pKa = 4.04TGTAYY852 pKa = 9.71TVTATDD858 pKa = 4.21ANTITYY864 pKa = 10.55SLGSGNDD871 pKa = 3.0EE872 pKa = 4.04ALFDD876 pKa = 4.1IVGSTGVVTFKK887 pKa = 10.64AAPDD891 pKa = 3.85FEE893 pKa = 4.98SPADD897 pKa = 3.81ADD899 pKa = 3.65EE900 pKa = 4.93DD901 pKa = 3.67NAYY904 pKa = 10.02LINVIASDD912 pKa = 4.1GVNAANQNVTITVTDD927 pKa = 3.35VDD929 pKa = 3.92DD930 pKa = 4.3TDD932 pKa = 4.46PEE934 pKa = 4.5FTSATAVNFAEE945 pKa = 4.46NEE947 pKa = 3.96TGTAYY952 pKa = 9.71TVTATDD958 pKa = 4.16ANTVTYY964 pKa = 10.84SLGSGNDD971 pKa = 3.0EE972 pKa = 4.04ALFDD976 pKa = 4.11IVSSTGVVTFKK987 pKa = 10.02TAPDD991 pKa = 3.89FEE993 pKa = 5.23SPADD997 pKa = 3.7TDD999 pKa = 3.13EE1000 pKa = 4.83DD1001 pKa = 3.4NAYY1004 pKa = 9.74VINVIASDD1012 pKa = 3.91GVNTVNQNVTITVTDD1027 pKa = 3.31VDD1029 pKa = 3.78DD1030 pKa = 4.21TDD1032 pKa = 3.9PVFTSVTAVNFAEE1045 pKa = 4.42NEE1047 pKa = 3.96TGTAYY1052 pKa = 9.71TVTATDD1058 pKa = 4.16ANTVTYY1064 pKa = 10.84SLGSGNDD1071 pKa = 3.0EE1072 pKa = 4.04ALFDD1076 pKa = 4.1IVGSTGVVTFKK1087 pKa = 10.07TAPDD1091 pKa = 3.89FEE1093 pKa = 5.17NPADD1097 pKa = 4.06ADD1099 pKa = 3.66EE1100 pKa = 5.03DD1101 pKa = 3.69NAYY1104 pKa = 10.02LINVIASDD1112 pKa = 3.93GVNTVNQNVTITVTDD1127 pKa = 3.28VDD1129 pKa = 3.6DD1130 pKa = 4.24TEE1132 pKa = 4.92PVFSSTTAVNFAEE1145 pKa = 4.46NEE1147 pKa = 3.96TGTAYY1152 pKa = 9.71TVTATDD1158 pKa = 3.91ANTITYY1164 pKa = 10.17CLGSGNDD1171 pKa = 3.27EE1172 pKa = 4.73SIFNIISTSGVVTFKK1187 pKa = 10.71AAPDD1191 pKa = 3.87FEE1193 pKa = 5.43SPADD1197 pKa = 3.7TDD1199 pKa = 3.13EE1200 pKa = 4.83DD1201 pKa = 3.4NAYY1204 pKa = 9.74VINVIASDD1212 pKa = 3.91GVNTVNQNVTITVTDD1227 pKa = 3.35VDD1229 pKa = 3.75DD1230 pKa = 4.29TNPVFTSVTTASFIEE1245 pKa = 4.48NEE1247 pKa = 4.04TGTAYY1252 pKa = 9.71TVTATDD1258 pKa = 4.21ANTITYY1264 pKa = 10.55SLGSGNDD1271 pKa = 3.1EE1272 pKa = 4.7SIFNIISTSGVVTFKK1287 pKa = 10.04TAPDD1291 pKa = 3.89FEE1293 pKa = 5.23SPADD1297 pKa = 3.61TDD1299 pKa = 3.3EE1300 pKa = 5.48DD1301 pKa = 3.42NDD1303 pKa = 3.77YY1304 pKa = 10.71VINVIASDD1312 pKa = 3.6GGNTVNQNVTITVTDD1327 pKa = 3.31VDD1329 pKa = 3.78DD1330 pKa = 4.21TDD1332 pKa = 4.05PVFTSATTASFIEE1345 pKa = 4.5NEE1347 pKa = 4.04TGTAYY1352 pKa = 9.71TVTATDD1358 pKa = 4.21ANTITYY1364 pKa = 10.55SLGSGNDD1371 pKa = 3.0EE1372 pKa = 4.04ALFDD1376 pKa = 4.1IVGSTGVVTFKK1387 pKa = 10.0TAPNFEE1393 pKa = 4.68SPADD1397 pKa = 3.6TDD1399 pKa = 3.46ADD1401 pKa = 3.36NDD1403 pKa = 3.83YY1404 pKa = 10.43VINVIASDD1412 pKa = 3.91GVNTVNQNVTITVTDD1427 pKa = 3.33VDD1429 pKa = 4.3DD1430 pKa = 5.18VITATDD1436 pKa = 3.58SYY1438 pKa = 11.29EE1439 pKa = 3.89HH1440 pKa = 6.96ALNDD1444 pKa = 2.93ISLYY1448 pKa = 10.37PNPTHH1453 pKa = 7.05DD1454 pKa = 4.3QLNIDD1459 pKa = 4.31LSNYY1463 pKa = 9.04QNSNLSVRR1471 pKa = 11.84ITNVSGAQYY1480 pKa = 10.6FLRR1483 pKa = 11.84EE1484 pKa = 4.09NIATQRR1490 pKa = 11.84LLVNVEE1496 pKa = 4.4KK1497 pKa = 10.85YY1498 pKa = 10.06PSGVYY1503 pKa = 10.04LVILQTANHH1512 pKa = 5.93MVTKK1516 pKa = 10.27RR1517 pKa = 11.84VIIAPP1522 pKa = 3.91

Molecular weight: 158.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5L603|A0A2G5L603_9BACT Uncharacterized protein OS=Reichenbachiella sp. 5M10 OX=1889772 GN=BFP72_17490 PE=3 SV=1
MM1 pKa = 7.76AVRR4 pKa = 11.84KK5 pKa = 9.07LKK7 pKa = 10.43PITPGTRR14 pKa = 11.84YY15 pKa = 9.08RR16 pKa = 11.84VAPVFEE22 pKa = 5.67EE23 pKa = 4.18ITKK26 pKa = 8.26STPEE30 pKa = 3.6KK31 pKa = 10.52SLTKK35 pKa = 10.12GVHH38 pKa = 5.92RR39 pKa = 11.84NGGRR43 pKa = 11.84NDD45 pKa = 3.49SGKK48 pKa = 8.29MTIRR52 pKa = 11.84NVGGGHH58 pKa = 5.04KK59 pKa = 9.85RR60 pKa = 11.84KK61 pKa = 9.94YY62 pKa = 8.95RR63 pKa = 11.84TIDD66 pKa = 3.54FKK68 pKa = 10.96RR69 pKa = 11.84DD70 pKa = 2.99KK71 pKa = 10.67HH72 pKa = 6.35GIPAVVKK79 pKa = 10.39SIEE82 pKa = 4.04YY83 pKa = 10.84DD84 pKa = 3.28PMRR87 pKa = 11.84TARR90 pKa = 11.84IALLFYY96 pKa = 10.97ADD98 pKa = 3.49GEE100 pKa = 4.19KK101 pKa = 10.26RR102 pKa = 11.84YY103 pKa = 10.22IIAPHH108 pKa = 5.82GLKK111 pKa = 10.37VGQTVLSGSGVAPEE125 pKa = 4.42VGNTLPLSDD134 pKa = 4.77IPLGTIIHH142 pKa = 5.76NVEE145 pKa = 4.23LKK147 pKa = 9.92PGKK150 pKa = 9.21GACIARR156 pKa = 11.84SAGNYY161 pKa = 7.81VQLLARR167 pKa = 11.84EE168 pKa = 4.42GKK170 pKa = 9.36YY171 pKa = 9.1ATVKK175 pKa = 10.57LPSGEE180 pKa = 3.7MRR182 pKa = 11.84MILVVCLATIGTVSNGDD199 pKa = 3.28HH200 pKa = 6.22MNVVLGKK207 pKa = 10.26AGRR210 pKa = 11.84NRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84SRR219 pKa = 11.84VRR221 pKa = 11.84GVAMNPVDD229 pKa = 3.68HH230 pKa = 7.13PMGGGEE236 pKa = 4.28GKK238 pKa = 10.51SSGGHH243 pKa = 4.59PRR245 pKa = 11.84SRR247 pKa = 11.84NGIFAKK253 pKa = 10.11GQKK256 pKa = 8.3TRR258 pKa = 11.84TPKK261 pKa = 10.31KK262 pKa = 9.23YY263 pKa = 9.95SNKK266 pKa = 10.06FIIGKK271 pKa = 9.41NKK273 pKa = 10.09

Molecular weight: 29.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3696

0

3696

1385859

54

5295

375.0

42.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.934 ± 0.036

0.742 ± 0.012

6.079 ± 0.035

6.72 ± 0.043

4.602 ± 0.028

6.962 ± 0.046

2.056 ± 0.022

6.865 ± 0.037

5.987 ± 0.057

9.407 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.019

4.892 ± 0.038

3.511 ± 0.019

3.98 ± 0.029

3.878 ± 0.032

6.886 ± 0.039

5.783 ± 0.063

6.774 ± 0.039

1.22 ± 0.018

4.236 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski