Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B1J2H5|A0A3B1J2H5_ASTMX Uncharacterized protein OS=Astyanax mexicanus OX=7994 PE=4 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.11DD4 pKa = 5.21HH5 pKa = 6.73GLCDD9 pKa = 3.86GNGPIDD15 pKa = 3.62VTEE18 pKa = 4.08SLEE21 pKa = 4.12LLLLAVTDD29 pKa = 4.4YY30 pKa = 11.12IVLLDD35 pKa = 4.25GVQCLLFTFQLDD47 pKa = 3.98DD48 pKa = 3.63VGVRR52 pKa = 11.84DD53 pKa = 5.05DD54 pKa = 5.56ALIQHH59 pKa = 6.18KK60 pKa = 10.17HH61 pKa = 4.87IDD63 pKa = 3.66FLGVDD68 pKa = 4.0KK69 pKa = 11.11PEE71 pKa = 4.46LLAPVQDD78 pKa = 4.18CSRR81 pKa = 11.84GANHH85 pKa = 6.8NLLLNTAASSDD96 pKa = 3.64LSEE99 pKa = 4.61EE100 pKa = 4.87LPLDD104 pKa = 3.67WLCSLDD110 pKa = 3.75YY111 pKa = 10.88FF112 pKa = 5.15

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B1KIV2|A0A3B1KIV2_ASTMX 40S ribosomal protein S6 OS=Astyanax mexicanus OX=7994 PE=3 SV=1
SS1 pKa = 7.19RR2 pKa = 11.84PGLRR6 pKa = 11.84AGSRR10 pKa = 11.84PGLRR14 pKa = 11.84AGSRR18 pKa = 11.84PGLRR22 pKa = 11.84AGSRR26 pKa = 11.84PGLRR30 pKa = 11.84AGSRR34 pKa = 11.84PGLRR38 pKa = 11.84AGSRR42 pKa = 11.84PGLRR46 pKa = 11.84AGSRR50 pKa = 11.84PGLRR54 pKa = 11.84AGSRR58 pKa = 11.84PGLRR62 pKa = 11.84AGSRR66 pKa = 11.84PGLRR70 pKa = 11.84AGSRR74 pKa = 11.84PGLRR78 pKa = 11.84AGSRR82 pKa = 11.84PGLRR86 pKa = 11.84AGSRR90 pKa = 11.84PGLRR94 pKa = 11.84AGSRR98 pKa = 11.84PGLRR102 pKa = 11.84AGSRR106 pKa = 11.84PGLRR110 pKa = 11.84AGSRR114 pKa = 11.84PGLRR118 pKa = 11.84AGSRR122 pKa = 11.84PGLRR126 pKa = 11.84AGSRR130 pKa = 11.84PGLRR134 pKa = 11.84AGSRR138 pKa = 11.84PGLRR142 pKa = 11.84AGSRR146 pKa = 11.84HH147 pKa = 6.31CSHH150 pKa = 6.78CVWGQKK156 pKa = 10.4LNN158 pKa = 3.65

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26658

12725

39383

22005327

18

8606

558.8

62.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.495 ± 0.013

2.317 ± 0.011

5.045 ± 0.008

6.895 ± 0.016

3.643 ± 0.01

6.172 ± 0.016

2.657 ± 0.009

4.422 ± 0.01

5.721 ± 0.018

9.494 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.005

3.859 ± 0.007

5.778 ± 0.02

4.747 ± 0.012

5.623 ± 0.012

8.829 ± 0.018

5.703 ± 0.013

6.368 ± 0.011

1.154 ± 0.005

2.786 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski