Streptococcus satellite phage Javan204

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKM2|A0A4D5ZKM2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan204 OX=2558566 GN=JavanS204_0009 PE=4 SV=1
MM1 pKa = 7.57LVRR4 pKa = 11.84LEE6 pKa = 4.24FSDD9 pKa = 4.86GNTLTRR15 pKa = 11.84MVSKK19 pKa = 9.15HH20 pKa = 5.01TLDD23 pKa = 4.03MILARR28 pKa = 11.84PYY30 pKa = 10.92SEE32 pKa = 3.92TWLHH36 pKa = 6.67FNGEE40 pKa = 4.28KK41 pKa = 10.04IAVLEE46 pKa = 4.01NVEE49 pKa = 4.1VLGNVDD55 pKa = 4.92DD56 pKa = 5.97FDD58 pKa = 5.52NEE60 pKa = 4.21VNKK63 pKa = 10.09TIAYY67 pKa = 9.79NEE69 pKa = 4.4LEE71 pKa = 4.35NEE73 pKa = 4.27VQEE76 pKa = 4.35NDD78 pKa = 3.08

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZI60|A0A4D5ZI60_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan204 OX=2558566 GN=JavanS204_0004 PE=4 SV=1
MM1 pKa = 8.09AEE3 pKa = 3.91IKK5 pKa = 10.55KK6 pKa = 8.29ITKK9 pKa = 10.26KK10 pKa = 10.74NGTTVYY16 pKa = 10.04RR17 pKa = 11.84QQIYY21 pKa = 10.8LGTDD25 pKa = 3.14CMTGKK30 pKa = 9.72QVYY33 pKa = 8.65TSISAPTKK41 pKa = 10.55KK42 pKa = 9.92EE43 pKa = 3.83LKK45 pKa = 9.55QKK47 pKa = 10.85RR48 pKa = 11.84EE49 pKa = 3.75FKK51 pKa = 10.77INEE54 pKa = 3.93FKK56 pKa = 11.26DD57 pKa = 3.34NGFTRR62 pKa = 11.84YY63 pKa = 10.24KK64 pKa = 10.46SVTVKK69 pKa = 10.57NYY71 pKa = 10.67RR72 pKa = 11.84EE73 pKa = 4.21LSEE76 pKa = 5.18LWLKK80 pKa = 8.48NHH82 pKa = 6.28KK83 pKa = 10.74LEE85 pKa = 4.9VKK87 pKa = 9.49PQTYY91 pKa = 9.64SQTVSEE97 pKa = 4.98LRR99 pKa = 11.84THH101 pKa = 7.23LLPVFGDD108 pKa = 3.42MKK110 pKa = 10.62VEE112 pKa = 5.01RR113 pKa = 11.84ITLPMVQEE121 pKa = 4.52FVNKK125 pKa = 9.93LASNDD130 pKa = 3.17KK131 pKa = 10.38LGRR134 pKa = 11.84VSFRR138 pKa = 11.84IILSINKK145 pKa = 9.73RR146 pKa = 11.84ILKK149 pKa = 9.77YY150 pKa = 10.46AVNLQIINVNPADD163 pKa = 3.78NVIVPKK169 pKa = 10.45NKK171 pKa = 10.13KK172 pKa = 9.92NISKK176 pKa = 10.5KK177 pKa = 10.08KK178 pKa = 7.76EE179 pKa = 3.74LKK181 pKa = 10.58FFEE184 pKa = 4.34TSQLKK189 pKa = 9.16QFKK192 pKa = 10.04DD193 pKa = 3.91YY194 pKa = 11.32LDD196 pKa = 4.06SLPNTFKK203 pKa = 11.05NYY205 pKa = 7.3YY206 pKa = 9.97HH207 pKa = 6.4KK208 pKa = 9.53TLYY211 pKa = 9.35LTLLSTGLRR220 pKa = 11.84IGEE223 pKa = 4.06AVALEE228 pKa = 4.23WSDD231 pKa = 3.49IDD233 pKa = 5.42LDD235 pKa = 3.87NGYY238 pKa = 9.85IDD240 pKa = 3.68VNKK243 pKa = 9.27TVAFSRR249 pKa = 11.84MEE251 pKa = 4.17TNSTKK256 pKa = 10.52SEE258 pKa = 3.8AGNRR262 pKa = 11.84KK263 pKa = 9.21ISIDD267 pKa = 3.55KK268 pKa = 9.11NTVLMLRR275 pKa = 11.84LYY277 pKa = 10.46KK278 pKa = 10.3ARR280 pKa = 11.84QYY282 pKa = 10.61QCFMEE287 pKa = 4.79HH288 pKa = 6.9GYY290 pKa = 9.46SSKK293 pKa = 8.75MAKK296 pKa = 9.98YY297 pKa = 10.11VFSNGFNIYY306 pKa = 9.94PNRR309 pKa = 11.84TNLQLVLTKK318 pKa = 10.25HH319 pKa = 6.32LKK321 pKa = 9.26QAGLPRR327 pKa = 11.84FTFHH331 pKa = 7.9AFRR334 pKa = 11.84HH335 pKa = 4.69THH337 pKa = 6.92ASLLLNAGISYY348 pKa = 10.65KK349 pKa = 10.3EE350 pKa = 3.81LQHH353 pKa = 6.91RR354 pKa = 11.84LGHH357 pKa = 5.56STLAMTMDD365 pKa = 4.15IYY367 pKa = 11.72SHH369 pKa = 6.87LSKK372 pKa = 10.74EE373 pKa = 4.21KK374 pKa = 10.83EE375 pKa = 3.53KK376 pKa = 11.22DD377 pKa = 3.16AVNFFEE383 pKa = 5.73KK384 pKa = 10.83AMANLL389 pKa = 3.66

Molecular weight: 45.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2172

43

504

167.1

19.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.88 ± 0.586

0.691 ± 0.16

5.203 ± 0.394

8.886 ± 0.808

3.775 ± 0.292

4.328 ± 0.536

1.565 ± 0.27

6.722 ± 0.538

10.175 ± 0.491

10.267 ± 0.688

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.443

6.308 ± 0.345

3.269 ± 0.599

3.683 ± 0.384

4.558 ± 0.397

5.663 ± 0.481

6.262 ± 0.417

6.077 ± 0.371

0.921 ± 0.205

4.144 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski