Escherichia phage Henu7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8RNT0|A0A5B8RNT0_9CAUD Uncharacterized protein OS=Escherichia phage Henu7 OX=2589652 PE=4 SV=1
MM1 pKa = 7.53SNEE4 pKa = 4.92KK5 pKa = 9.6IDD7 pKa = 3.68QLVDD11 pKa = 4.22EE12 pKa = 5.73IIEE15 pKa = 4.19THH17 pKa = 7.18DD18 pKa = 3.48YY19 pKa = 11.68AGFNSIFMIYY29 pKa = 9.72PDD31 pKa = 3.65EE32 pKa = 5.24CGVTEE37 pKa = 4.45SEE39 pKa = 4.1LKK41 pKa = 10.52EE42 pKa = 3.89AVEE45 pKa = 4.14QVEE48 pKa = 4.71SVLRR52 pKa = 11.84TDD54 pKa = 4.35NPDD57 pKa = 3.52DD58 pKa = 4.87EE59 pKa = 5.14IFSSLTACDD68 pKa = 3.57GGGVFALSFINANTILTVV86 pKa = 3.19

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8RLM2|A0A5B8RLM2_9CAUD Putative head-tail adaptor OS=Escherichia phage Henu7 OX=2589652 PE=4 SV=1
MM1 pKa = 7.56ILGFVIGFIAAIVMVMVIGGAAQKK25 pKa = 10.57YY26 pKa = 9.29AIKK29 pKa = 10.48KK30 pKa = 8.96GWFASAIWIEE40 pKa = 4.25KK41 pKa = 7.77EE42 pKa = 3.92KK43 pKa = 10.15RR44 pKa = 11.84WKK46 pKa = 9.92VRR48 pKa = 11.84GRR50 pKa = 11.84FLSVASKK57 pKa = 9.76IHH59 pKa = 5.44TGILEE64 pKa = 4.22EE65 pKa = 4.21YY66 pKa = 9.52RR67 pKa = 11.84SGRR70 pKa = 11.84KK71 pKa = 5.66TVKK74 pKa = 10.69YY75 pKa = 10.72VDD77 pKa = 3.25

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13400

55

1178

200.0

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.157 ± 0.783

1.261 ± 0.198

6.142 ± 0.204

6.91 ± 0.297

3.799 ± 0.194

7.53 ± 0.26

1.56 ± 0.22

6.172 ± 0.221

6.888 ± 0.304

6.679 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.201 ± 0.18

4.493 ± 0.245

3.261 ± 0.238

3.978 ± 0.277

5.127 ± 0.212

6.328 ± 0.342

5.522 ± 0.273

6.963 ± 0.343

1.53 ± 0.131

3.5 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski