Gordonia Phage Zitch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3V9K9|A0A7G3V9K9_9CAUD Uncharacterized protein OS=Gordonia Phage Zitch OX=2743909 GN=76 PE=4 SV=1
MM1 pKa = 7.98IIRR4 pKa = 11.84KK5 pKa = 7.1TLAAAALTLGALTAIAAPAAAVNLDD30 pKa = 3.25AYY32 pKa = 10.18DD33 pKa = 3.58TVRR36 pKa = 11.84YY37 pKa = 7.23MVCGDD42 pKa = 3.63GVAEE46 pKa = 4.22ISYY49 pKa = 10.56IDD51 pKa = 3.36VDD53 pKa = 4.43GNFDD57 pKa = 4.16EE58 pKa = 5.8DD59 pKa = 3.86WADD62 pKa = 3.67LSQGCWFYY70 pKa = 11.84DD71 pKa = 3.46MNVGSDD77 pKa = 3.44GYY79 pKa = 10.67GYY81 pKa = 9.75PDD83 pKa = 3.45GTSYY87 pKa = 11.7ASVIGVDD94 pKa = 3.75DD95 pKa = 3.82NGGRR99 pKa = 11.84VSCTVWVNGHH109 pKa = 6.47IEE111 pKa = 4.27ADD113 pKa = 3.38VDD115 pKa = 3.9DD116 pKa = 4.49TSDD119 pKa = 3.78YY120 pKa = 11.12YY121 pKa = 11.67SWASCDD127 pKa = 3.23

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3V997|A0A7G3V997_9CAUD Uncharacterized protein OS=Gordonia Phage Zitch OX=2743909 GN=87 PE=4 SV=1
MM1 pKa = 7.36SRR3 pKa = 11.84SKK5 pKa = 11.21LPGRR9 pKa = 11.84QITRR13 pKa = 11.84TLRR16 pKa = 11.84VWAGSRR22 pKa = 11.84GISVWWKK29 pKa = 9.43RR30 pKa = 11.84PDD32 pKa = 3.82GKK34 pKa = 9.48WHH36 pKa = 5.76FRR38 pKa = 11.84IDD40 pKa = 2.78RR41 pKa = 11.84WSPHH45 pKa = 5.26GAMPPPGCSWKK56 pKa = 9.81PWPHH60 pKa = 5.83RR61 pKa = 11.84QLHH64 pKa = 6.51RR65 pKa = 11.84DD66 pKa = 3.47LEE68 pKa = 4.31QTFIEE73 pKa = 4.95IYY75 pKa = 8.55WPTVGSIHH83 pKa = 6.41ATRR86 pKa = 11.84FYY88 pKa = 9.26NTSYY92 pKa = 10.83VFDD95 pKa = 3.83YY96 pKa = 10.96RR97 pKa = 11.84GDD99 pKa = 3.74PMSLRR104 pKa = 11.84DD105 pKa = 3.44RR106 pKa = 11.84WVKK109 pKa = 10.01RR110 pKa = 11.84AEE112 pKa = 4.03RR113 pKa = 11.84PKK115 pKa = 10.23PARR118 pKa = 11.84AYY120 pKa = 10.52QLVFYY125 pKa = 9.37PAADD129 pKa = 3.51QPDD132 pKa = 3.63GMRR135 pKa = 11.84YY136 pKa = 9.12AVPQLLGDD144 pKa = 3.92YY145 pKa = 7.45PTYY148 pKa = 10.6AAAVRR153 pKa = 11.84EE154 pKa = 4.25LEE156 pKa = 4.23FARR159 pKa = 11.84DD160 pKa = 3.21RR161 pKa = 11.84QTRR164 pKa = 11.84RR165 pKa = 11.84GYY167 pKa = 9.36WRR169 pKa = 11.84IEE171 pKa = 3.85PTSS174 pKa = 3.3

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

18529

28

1642

203.6

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.354 ± 0.398

0.917 ± 0.148

7.469 ± 0.489

5.516 ± 0.322

2.24 ± 0.204

8.398 ± 0.458

2.051 ± 0.217

4.62 ± 0.266

2.666 ± 0.183

7.604 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.124

2.752 ± 0.214

6.493 ± 0.314

3.767 ± 0.156

7.599 ± 0.437

4.512 ± 0.257

7.151 ± 0.295

7.259 ± 0.185

2.132 ± 0.146

2.207 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski