Beihai sobemo-like virus 20

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE67|A0A1L3KE67_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 20 OX=1922692 PE=4 SV=1
MM1 pKa = 7.71LDD3 pKa = 3.45SRR5 pKa = 11.84FGVQKK10 pKa = 10.68QEE12 pKa = 4.99SEE14 pKa = 4.23DD15 pKa = 3.74FLNKK19 pKa = 9.68ILEE22 pKa = 4.36SDD24 pKa = 4.0DD25 pKa = 3.63IEE27 pKa = 4.38NVEE30 pKa = 4.92SFVEE34 pKa = 4.2PNFDD38 pKa = 2.94EE39 pKa = 4.78GFRR42 pKa = 11.84VKK44 pKa = 10.45DD45 pKa = 3.55RR46 pKa = 11.84KK47 pKa = 10.7GRR49 pKa = 11.84THH51 pKa = 6.5VFWSDD56 pKa = 3.21NITDD60 pKa = 4.32EE61 pKa = 5.45IVTKK65 pKa = 10.76LNKK68 pKa = 9.53KK69 pKa = 8.81SKK71 pKa = 9.2KK72 pKa = 10.12ASYY75 pKa = 9.57TLTPVQLQSAAQIQPQAADD94 pKa = 3.46NPTPKK99 pKa = 9.39DD100 pKa = 3.39TKK102 pKa = 10.91LSDD105 pKa = 3.5SEE107 pKa = 4.03IQLRR111 pKa = 11.84EE112 pKa = 4.06QINEE116 pKa = 3.84LTKK119 pKa = 10.64EE120 pKa = 4.15VTKK123 pKa = 10.87LRR125 pKa = 11.84EE126 pKa = 3.79EE127 pKa = 4.04QKK129 pKa = 11.11ALNVQPQRR137 pKa = 11.84PVANVDD143 pKa = 3.38YY144 pKa = 10.83QQVQEE149 pKa = 4.5AQVCMGLYY157 pKa = 9.92QRR159 pKa = 11.84ATQILTLAASLQQFLSGHH177 pKa = 5.67FRR179 pKa = 11.84DD180 pKa = 3.87EE181 pKa = 4.01PARR184 pKa = 11.84VKK186 pKa = 10.62QFAANQQLINGVLNQLAQVGYY207 pKa = 11.14AMSAKK212 pKa = 7.74MTTIAQLSDD221 pKa = 3.52EE222 pKa = 4.71DD223 pKa = 3.97LQNKK227 pKa = 7.98NRR229 pKa = 11.84ADD231 pKa = 4.06RR232 pKa = 11.84QRR234 pKa = 11.84KK235 pKa = 8.44KK236 pKa = 10.73IPMPEE241 pKa = 3.8ADD243 pKa = 3.41EE244 pKa = 4.18EE245 pKa = 4.45TRR247 pKa = 11.84NMIEE251 pKa = 4.44EE252 pKa = 4.28ALQFANEE259 pKa = 3.88GFEE262 pKa = 4.35RR263 pKa = 11.84IEE265 pKa = 4.66AIPDD269 pKa = 3.39RR270 pKa = 11.84LYY272 pKa = 10.63PDD274 pKa = 3.81YY275 pKa = 10.77AAPAPPVEE283 pKa = 4.26PQGASDD289 pKa = 4.74YY290 pKa = 10.61ADD292 pKa = 3.84CMAKK296 pKa = 9.97KK297 pKa = 10.47YY298 pKa = 10.48SVQIPTIFQLGSAADD313 pKa = 4.01FLSHH317 pKa = 7.21KK318 pKa = 10.48ASQTQQSEE326 pKa = 4.07GRR328 pKa = 11.84KK329 pKa = 9.54NIRR332 pKa = 11.84TEE334 pKa = 3.94KK335 pKa = 9.03PQMSEE340 pKa = 4.09VEE342 pKa = 4.31KK343 pKa = 10.08QTATLMSNFPGLSKK357 pKa = 10.66KK358 pKa = 10.55LSPTQVATSGPKK370 pKa = 8.35PTEE373 pKa = 3.86PQLPPVSPSTHH384 pKa = 6.35KK385 pKa = 10.87EE386 pKa = 3.75EE387 pKa = 4.45PKK389 pKa = 9.49PAKK392 pKa = 9.28TAWSQQKK399 pKa = 8.97MSEE402 pKa = 4.1PSVQDD407 pKa = 3.05VCANAIEE414 pKa = 4.42MLSATSSQSDD424 pKa = 3.59SLAQALSLLKK434 pKa = 10.62KK435 pKa = 9.44MQLEE439 pKa = 4.14NEE441 pKa = 4.38KK442 pKa = 10.42EE443 pKa = 4.22VEE445 pKa = 4.18SLQEE449 pKa = 3.74EE450 pKa = 4.69GLPRR454 pKa = 11.84SEE456 pKa = 4.34EE457 pKa = 3.89LRR459 pKa = 11.84EE460 pKa = 4.21DD461 pKa = 3.87EE462 pKa = 4.18EE463 pKa = 5.47DD464 pKa = 3.45EE465 pKa = 4.99FILVEE470 pKa = 4.0PQKK473 pKa = 11.26VKK475 pKa = 11.04DD476 pKa = 3.73MKK478 pKa = 10.63KK479 pKa = 10.49CPACHH484 pKa = 6.15SKK486 pKa = 10.45VKK488 pKa = 10.73KK489 pKa = 10.11GDD491 pKa = 2.93TWEE494 pKa = 4.05DD495 pKa = 3.28HH496 pKa = 6.75KK497 pKa = 11.32KK498 pKa = 10.17QPCFVEE504 pKa = 4.51WSKK507 pKa = 11.29KK508 pKa = 9.97RR509 pKa = 11.84PPLNTNKK516 pKa = 9.36VPRR519 pKa = 11.84PKK521 pKa = 8.3TTAA524 pKa = 3.01

Molecular weight: 59.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE67|A0A1L3KE67_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 20 OX=1922692 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 4.06MVVDD6 pKa = 4.36PTTSPGLPWTSIGTTNAQVFGYY28 pKa = 9.91QEE30 pKa = 4.72GIGFRR35 pKa = 11.84GQQVEE40 pKa = 4.14ILYY43 pKa = 10.55KK44 pKa = 10.47EE45 pKa = 4.27VILRR49 pKa = 11.84LQQLRR54 pKa = 11.84EE55 pKa = 4.15GPKK58 pKa = 9.94MNDD61 pKa = 2.36INLFIKK67 pKa = 10.35PEE69 pKa = 3.69PHH71 pKa = 6.39KK72 pKa = 10.29PKK74 pKa = 10.4KK75 pKa = 10.01VEE77 pKa = 3.62QGAWRR82 pKa = 11.84LIHH85 pKa = 6.31GVSITDD91 pKa = 3.24QMVAEE96 pKa = 4.21IVMGEE101 pKa = 4.29LMDD104 pKa = 5.68LVYY107 pKa = 10.55KK108 pKa = 10.84CPGRR112 pKa = 11.84YY113 pKa = 9.71GPMIGWSPFTTEE125 pKa = 4.89GIPYY129 pKa = 10.17FNFFVGNGPKK139 pKa = 9.89QSADD143 pKa = 3.1KK144 pKa = 11.17SSWDD148 pKa = 3.13WTVQDD153 pKa = 3.36WLAEE157 pKa = 4.06VFIDD161 pKa = 5.15LICYY165 pKa = 9.43IQDD168 pKa = 4.3DD169 pKa = 4.37LNEE172 pKa = 4.33TCSTQLRR179 pKa = 11.84NHH181 pKa = 5.52MRR183 pKa = 11.84AILGRR188 pKa = 11.84KK189 pKa = 8.7VIVGKK194 pKa = 8.01GQRR197 pKa = 11.84FTMDD201 pKa = 2.76GGLPSGWKK209 pKa = 8.47ATIFANSVMQEE220 pKa = 4.27IIHH223 pKa = 6.07HH224 pKa = 4.97VAEE227 pKa = 4.71KK228 pKa = 10.66RR229 pKa = 11.84SGCYY233 pKa = 9.44GKK235 pKa = 9.84PPITMGDD242 pKa = 3.55DD243 pKa = 3.54TLQEE247 pKa = 4.28PMPPAYY253 pKa = 9.27WEE255 pKa = 4.16ALATTGCILKK265 pKa = 10.39EE266 pKa = 4.28VVTHH270 pKa = 6.06QDD272 pKa = 2.6RR273 pKa = 11.84PYY275 pKa = 9.49EE276 pKa = 3.81FCGFHH281 pKa = 6.08FGSRR285 pKa = 11.84EE286 pKa = 3.77YY287 pKa = 10.84EE288 pKa = 3.49PSYY291 pKa = 9.39KK292 pKa = 9.81QKK294 pKa = 10.1HH295 pKa = 5.4AFLVRR300 pKa = 11.84HH301 pKa = 5.92IEE303 pKa = 4.01RR304 pKa = 11.84EE305 pKa = 4.02NFEE308 pKa = 4.23ATIQSYY314 pKa = 10.71QILYY318 pKa = 10.44LFDD321 pKa = 3.62EE322 pKa = 4.69RR323 pKa = 11.84RR324 pKa = 11.84LNALRR329 pKa = 11.84RR330 pKa = 11.84WCEE333 pKa = 3.7LEE335 pKa = 5.05GFAHH339 pKa = 6.62CKK341 pKa = 9.78IDD343 pKa = 3.51VSDD346 pKa = 3.35RR347 pKa = 11.84KK348 pKa = 10.3YY349 pKa = 10.9VVRR352 pKa = 11.84GTPEE356 pKa = 3.18

Molecular weight: 40.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

880

356

524

440.0

49.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.705 ± 1.287

1.591 ± 0.382

5.341 ± 0.166

8.636 ± 0.776

3.75 ± 0.597

5.114 ± 1.929

1.818 ± 0.577

5.0 ± 1.177

7.614 ± 1.325

7.273 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.308

3.636 ± 0.482

6.591 ± 0.403

7.614 ± 1.325

4.773 ± 0.492

6.25 ± 1.185

5.568 ± 0.029

5.909 ± 0.321

1.477 ± 0.612

2.5 ± 0.834
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski