Amino acid dipepetide frequency for Vibrio phage VAP7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.489AlaAla: 4.489 ± 0.341
0.689AlaCys: 0.689 ± 0.131
4.089AlaAsp: 4.089 ± 0.315
4.489AlaGlu: 4.489 ± 0.303
2.467AlaPhe: 2.467 ± 0.204
3.911AlaGly: 3.911 ± 0.383
1.289AlaHis: 1.289 ± 0.184
4.4AlaIle: 4.4 ± 0.337
4.978AlaLys: 4.978 ± 0.322
4.978AlaLeu: 4.978 ± 0.364
2.044AlaMet: 2.044 ± 0.226
3.356AlaAsn: 3.356 ± 0.321
2.089AlaPro: 2.089 ± 0.202
2.533AlaGln: 2.533 ± 0.29
2.956AlaArg: 2.956 ± 0.25
3.533AlaSer: 3.533 ± 0.28
3.4AlaThr: 3.4 ± 0.304
4.333AlaVal: 4.333 ± 0.337
0.889AlaTrp: 0.889 ± 0.122
2.089AlaTyr: 2.089 ± 0.215
0.0AlaXaa: 0.0 ± 0.0
Cys
0.689CysAla: 0.689 ± 0.134
0.333CysCys: 0.333 ± 0.102
0.867CysAsp: 0.867 ± 0.149
0.778CysGlu: 0.778 ± 0.127
0.333CysPhe: 0.333 ± 0.086
0.644CysGly: 0.644 ± 0.13
0.289CysHis: 0.289 ± 0.079
0.867CysIle: 0.867 ± 0.121
0.6CysLys: 0.6 ± 0.102
0.689CysLeu: 0.689 ± 0.131
0.311CysMet: 0.311 ± 0.071
0.733CysAsn: 0.733 ± 0.115
0.667CysPro: 0.667 ± 0.118
0.333CysGln: 0.333 ± 0.071
0.711CysArg: 0.711 ± 0.113
0.467CysSer: 0.467 ± 0.117
0.644CysThr: 0.644 ± 0.136
0.711CysVal: 0.711 ± 0.131
0.133CysTrp: 0.133 ± 0.049
0.511CysTyr: 0.511 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
3.889AspAla: 3.889 ± 0.326
0.867AspCys: 0.867 ± 0.143
4.022AspAsp: 4.022 ± 0.323
4.289AspGlu: 4.289 ± 0.355
2.711AspPhe: 2.711 ± 0.202
5.222AspGly: 5.222 ± 0.369
1.2AspHis: 1.2 ± 0.144
4.689AspIle: 4.689 ± 0.288
4.556AspLys: 4.556 ± 0.365
4.756AspLeu: 4.756 ± 0.292
2.4AspMet: 2.4 ± 0.265
3.622AspAsn: 3.622 ± 0.256
2.267AspPro: 2.267 ± 0.234
2.133AspGln: 2.133 ± 0.179
2.333AspArg: 2.333 ± 0.246
3.511AspSer: 3.511 ± 0.272
3.2AspThr: 3.2 ± 0.27
4.422AspVal: 4.422 ± 0.355
1.289AspTrp: 1.289 ± 0.18
2.845AspTyr: 2.845 ± 0.263
0.0AspXaa: 0.0 ± 0.0
Glu
4.889GluAla: 4.889 ± 0.415
0.867GluCys: 0.867 ± 0.143
4.133GluAsp: 4.133 ± 0.378
4.956GluGlu: 4.956 ± 0.384
3.356GluPhe: 3.356 ± 0.284
3.822GluGly: 3.822 ± 0.286
2.0GluHis: 2.0 ± 0.252
4.8GluIle: 4.8 ± 0.34
4.667GluLys: 4.667 ± 0.306
5.733GluLeu: 5.733 ± 0.38
2.111GluMet: 2.111 ± 0.208
3.822GluAsn: 3.822 ± 0.24
2.4GluPro: 2.4 ± 0.233
3.667GluGln: 3.667 ± 0.295
3.222GluArg: 3.222 ± 0.263
4.067GluSer: 4.067 ± 0.356
3.689GluThr: 3.689 ± 0.308
4.022GluVal: 4.022 ± 0.308
0.911GluTrp: 0.911 ± 0.133
2.889GluTyr: 2.889 ± 0.259
0.0GluXaa: 0.0 ± 0.0
Phe
2.711PheAla: 2.711 ± 0.264
0.244PheCys: 0.244 ± 0.067
3.289PheAsp: 3.289 ± 0.235
3.422PheGlu: 3.422 ± 0.311
1.578PhePhe: 1.578 ± 0.205
2.467PheGly: 2.467 ± 0.23
0.933PheHis: 0.933 ± 0.123
2.489PheIle: 2.489 ± 0.206
3.067PheLys: 3.067 ± 0.267
2.578PheLeu: 2.578 ± 0.244
1.511PheMet: 1.511 ± 0.183
2.556PheAsn: 2.556 ± 0.277
1.089PhePro: 1.089 ± 0.15
1.556PheGln: 1.556 ± 0.214
1.956PheArg: 1.956 ± 0.21
2.267PheSer: 2.267 ± 0.265
2.778PheThr: 2.778 ± 0.239
3.156PheVal: 3.156 ± 0.236
0.644PheTrp: 0.644 ± 0.124
1.422PheTyr: 1.422 ± 0.154
0.0PheXaa: 0.0 ± 0.0
Gly
3.245GlyAla: 3.245 ± 0.251
0.489GlyCys: 0.489 ± 0.131
4.289GlyAsp: 4.289 ± 0.286
4.289GlyGlu: 4.289 ± 0.384
2.933GlyPhe: 2.933 ± 0.225
4.245GlyGly: 4.245 ± 0.348
1.622GlyHis: 1.622 ± 0.179
4.356GlyIle: 4.356 ± 0.345
4.467GlyLys: 4.467 ± 0.378
5.4GlyLeu: 5.4 ± 0.293
2.289GlyMet: 2.289 ± 0.204
3.289GlyAsn: 3.289 ± 0.281
1.689GlyPro: 1.689 ± 0.201
2.511GlyGln: 2.511 ± 0.216
3.356GlyArg: 3.356 ± 0.312
3.911GlySer: 3.911 ± 0.35
3.956GlyThr: 3.956 ± 0.408
4.8GlyVal: 4.8 ± 0.351
0.956GlyTrp: 0.956 ± 0.158
2.711GlyTyr: 2.711 ± 0.266
0.0GlyXaa: 0.0 ± 0.0
His
1.156HisAla: 1.156 ± 0.154
0.444HisCys: 0.444 ± 0.093
1.044HisAsp: 1.044 ± 0.164
1.467HisGlu: 1.467 ± 0.17
0.978HisPhe: 0.978 ± 0.141
1.467HisGly: 1.467 ± 0.199
0.711HisHis: 0.711 ± 0.118
1.8HisIle: 1.8 ± 0.195
1.933HisLys: 1.933 ± 0.226
2.0HisLeu: 2.0 ± 0.254
0.733HisMet: 0.733 ± 0.125
1.244HisAsn: 1.244 ± 0.161
0.844HisPro: 0.844 ± 0.14
0.689HisGln: 0.689 ± 0.146
1.178HisArg: 1.178 ± 0.165
1.311HisSer: 1.311 ± 0.171
1.422HisThr: 1.422 ± 0.188
1.511HisVal: 1.511 ± 0.18
0.244HisTrp: 0.244 ± 0.081
1.222HisTyr: 1.222 ± 0.179
0.0HisXaa: 0.0 ± 0.0
Ile
4.422IleAla: 4.422 ± 0.316
0.578IleCys: 0.578 ± 0.114
4.556IleAsp: 4.556 ± 0.315
4.978IleGlu: 4.978 ± 0.353
2.244IlePhe: 2.244 ± 0.274
4.0IleGly: 4.0 ± 0.32
1.2IleHis: 1.2 ± 0.15
3.4IleIle: 3.4 ± 0.261
5.356IleLys: 5.356 ± 0.362
4.378IleLeu: 4.378 ± 0.319
1.756IleMet: 1.756 ± 0.218
4.245IleAsn: 4.245 ± 0.288
3.022IlePro: 3.022 ± 0.276
2.933IleGln: 2.933 ± 0.242
3.489IleArg: 3.489 ± 0.265
3.733IleSer: 3.733 ± 0.321
3.756IleThr: 3.756 ± 0.241
3.556IleVal: 3.556 ± 0.26
0.756IleTrp: 0.756 ± 0.135
2.289IleTyr: 2.289 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
5.022LysAla: 5.022 ± 0.335
0.911LysCys: 0.911 ± 0.165
4.778LysAsp: 4.778 ± 0.329
4.356LysGlu: 4.356 ± 0.311
2.8LysPhe: 2.8 ± 0.22
4.378LysGly: 4.378 ± 0.369
2.178LysHis: 2.178 ± 0.222
4.089LysIle: 4.089 ± 0.335
4.8LysLys: 4.8 ± 0.49
6.733LysLeu: 6.733 ± 0.403
2.289LysMet: 2.289 ± 0.238
3.489LysAsn: 3.489 ± 0.276
3.156LysPro: 3.156 ± 0.296
2.778LysGln: 2.778 ± 0.249
3.6LysArg: 3.6 ± 0.245
3.378LysSer: 3.378 ± 0.294
4.245LysThr: 4.245 ± 0.306
5.333LysVal: 5.333 ± 0.379
1.044LysTrp: 1.044 ± 0.151
3.111LysTyr: 3.111 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
5.111LeuAla: 5.111 ± 0.349
0.911LeuCys: 0.911 ± 0.177
5.289LeuAsp: 5.289 ± 0.346
6.8LeuGlu: 6.8 ± 0.371
3.356LeuPhe: 3.356 ± 0.311
4.333LeuGly: 4.333 ± 0.301
1.644LeuHis: 1.644 ± 0.19
4.378LeuIle: 4.378 ± 0.337
6.022LeuLys: 6.022 ± 0.353
5.867LeuLeu: 5.867 ± 0.46
2.711LeuMet: 2.711 ± 0.253
4.556LeuAsn: 4.556 ± 0.306
3.2LeuPro: 3.2 ± 0.256
2.556LeuGln: 2.556 ± 0.244
3.333LeuArg: 3.333 ± 0.298
4.622LeuSer: 4.622 ± 0.316
4.311LeuThr: 4.311 ± 0.285
5.178LeuVal: 5.178 ± 0.377
0.778LeuTrp: 0.778 ± 0.129
3.289LeuTyr: 3.289 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
2.622MetAla: 2.622 ± 0.265
0.311MetCys: 0.311 ± 0.085
1.933MetAsp: 1.933 ± 0.228
1.956MetGlu: 1.956 ± 0.184
1.311MetPhe: 1.311 ± 0.191
1.711MetGly: 1.711 ± 0.178
0.8MetHis: 0.8 ± 0.126
1.778MetIle: 1.778 ± 0.195
2.511MetLys: 2.511 ± 0.203
2.267MetLeu: 2.267 ± 0.229
1.089MetMet: 1.089 ± 0.176
1.844MetAsn: 1.844 ± 0.188
1.2MetPro: 1.2 ± 0.172
1.111MetGln: 1.111 ± 0.17
1.667MetArg: 1.667 ± 0.226
2.289MetSer: 2.289 ± 0.198
1.911MetThr: 1.911 ± 0.176
1.844MetVal: 1.844 ± 0.233
0.333MetTrp: 0.333 ± 0.08
1.044MetTyr: 1.044 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.445AsnAla: 3.445 ± 0.329
0.533AsnCys: 0.533 ± 0.105
2.733AsnAsp: 2.733 ± 0.271
3.333AsnGlu: 3.333 ± 0.229
2.467AsnPhe: 2.467 ± 0.309
4.867AsnGly: 4.867 ± 0.328
1.067AsnHis: 1.067 ± 0.167
3.911AsnIle: 3.911 ± 0.277
3.511AsnLys: 3.511 ± 0.305
4.156AsnLeu: 4.156 ± 0.276
1.733AsnMet: 1.733 ± 0.215
3.156AsnAsn: 3.156 ± 0.279
2.356AsnPro: 2.356 ± 0.231
1.956AsnGln: 1.956 ± 0.198
2.733AsnArg: 2.733 ± 0.211
2.845AsnSer: 2.845 ± 0.252
3.622AsnThr: 3.622 ± 0.343
4.311AsnVal: 4.311 ± 0.369
0.644AsnTrp: 0.644 ± 0.134
1.933AsnTyr: 1.933 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
1.711ProAla: 1.711 ± 0.197
0.333ProCys: 0.333 ± 0.083
2.4ProAsp: 2.4 ± 0.233
3.045ProGlu: 3.045 ± 0.23
1.533ProPhe: 1.533 ± 0.164
2.933ProGly: 2.933 ± 0.256
0.733ProHis: 0.733 ± 0.153
2.311ProIle: 2.311 ± 0.258
2.733ProLys: 2.733 ± 0.269
2.378ProLeu: 2.378 ± 0.283
0.933ProMet: 0.933 ± 0.138
2.156ProAsn: 2.156 ± 0.214
1.156ProPro: 1.156 ± 0.177
0.956ProGln: 0.956 ± 0.153
1.489ProArg: 1.489 ± 0.181
2.289ProSer: 2.289 ± 0.241
2.8ProThr: 2.8 ± 0.269
2.6ProVal: 2.6 ± 0.218
0.644ProTrp: 0.644 ± 0.105
1.378ProTyr: 1.378 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
2.645GlnAla: 2.645 ± 0.272
0.489GlnCys: 0.489 ± 0.091
1.956GlnAsp: 1.956 ± 0.203
2.889GlnGlu: 2.889 ± 0.295
1.867GlnPhe: 1.867 ± 0.169
2.067GlnGly: 2.067 ± 0.191
0.867GlnHis: 0.867 ± 0.14
2.645GlnIle: 2.645 ± 0.249
2.622GlnLys: 2.622 ± 0.23
3.222GlnLeu: 3.222 ± 0.203
1.289GlnMet: 1.289 ± 0.156
1.778GlnAsn: 1.778 ± 0.174
1.222GlnPro: 1.222 ± 0.232
1.489GlnGln: 1.489 ± 0.18
1.8GlnArg: 1.8 ± 0.189
1.956GlnSer: 1.956 ± 0.206
2.489GlnThr: 2.489 ± 0.198
2.756GlnVal: 2.756 ± 0.26
0.622GlnTrp: 0.622 ± 0.128
1.422GlnTyr: 1.422 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
2.4ArgAla: 2.4 ± 0.201
0.578ArgCys: 0.578 ± 0.108
2.756ArgAsp: 2.756 ± 0.23
2.956ArgGlu: 2.956 ± 0.347
1.978ArgPhe: 1.978 ± 0.2
3.111ArgGly: 3.111 ± 0.235
1.044ArgHis: 1.044 ± 0.151
3.489ArgIle: 3.489 ± 0.292
3.289ArgLys: 3.289 ± 0.272
3.778ArgLeu: 3.778 ± 0.287
1.444ArgMet: 1.444 ± 0.166
2.667ArgAsn: 2.667 ± 0.239
1.422ArgPro: 1.422 ± 0.161
1.933ArgGln: 1.933 ± 0.178
2.978ArgArg: 2.978 ± 0.261
2.533ArgSer: 2.533 ± 0.229
2.733ArgThr: 2.733 ± 0.346
3.578ArgVal: 3.578 ± 0.265
0.911ArgTrp: 0.911 ± 0.118
1.911ArgTyr: 1.911 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
3.311SerAla: 3.311 ± 0.289
0.6SerCys: 0.6 ± 0.118
3.022SerAsp: 3.022 ± 0.256
3.445SerGlu: 3.445 ± 0.282
2.356SerPhe: 2.356 ± 0.22
4.2SerGly: 4.2 ± 0.393
1.667SerHis: 1.667 ± 0.215
3.911SerIle: 3.911 ± 0.291
4.4SerLys: 4.4 ± 0.326
4.622SerLeu: 4.622 ± 0.255
1.578SerMet: 1.578 ± 0.177
3.467SerAsn: 3.467 ± 0.259
2.089SerPro: 2.089 ± 0.209
2.067SerGln: 2.067 ± 0.254
2.222SerArg: 2.222 ± 0.236
3.2SerSer: 3.2 ± 0.245
3.622SerThr: 3.622 ± 0.297
3.956SerVal: 3.956 ± 0.304
0.756SerTrp: 0.756 ± 0.133
2.067SerTyr: 2.067 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
3.556ThrAla: 3.556 ± 0.341
0.778ThrCys: 0.778 ± 0.155
3.556ThrAsp: 3.556 ± 0.308
3.911ThrGlu: 3.911 ± 0.295
2.778ThrPhe: 2.778 ± 0.268
4.422ThrGly: 4.422 ± 0.409
1.311ThrHis: 1.311 ± 0.176
3.467ThrIle: 3.467 ± 0.274
4.156ThrLys: 4.156 ± 0.35
5.756ThrLeu: 5.756 ± 0.367
1.644ThrMet: 1.644 ± 0.204
3.067ThrAsn: 3.067 ± 0.226
2.645ThrPro: 2.645 ± 0.316
1.6ThrGln: 1.6 ± 0.191
2.244ThrArg: 2.244 ± 0.212
3.8ThrSer: 3.8 ± 0.401
3.645ThrThr: 3.645 ± 0.383
4.267ThrVal: 4.267 ± 0.342
0.978ThrTrp: 0.978 ± 0.179
1.956ThrTyr: 1.956 ± 0.187
0.0ThrXaa: 0.0 ± 0.0
Val
4.022ValAla: 4.022 ± 0.271
0.689ValCys: 0.689 ± 0.121
5.356ValAsp: 5.356 ± 0.331
5.356ValGlu: 5.356 ± 0.378
2.489ValPhe: 2.489 ± 0.209
3.667ValGly: 3.667 ± 0.281
1.467ValHis: 1.467 ± 0.193
4.667ValIle: 4.667 ± 0.307
5.067ValLys: 5.067 ± 0.347
4.622ValLeu: 4.622 ± 0.343
2.022ValMet: 2.022 ± 0.218
3.778ValAsn: 3.778 ± 0.279
2.356ValPro: 2.356 ± 0.254
3.067ValGln: 3.067 ± 0.247
3.089ValArg: 3.089 ± 0.313
4.089ValSer: 4.089 ± 0.296
4.378ValThr: 4.378 ± 0.356
4.845ValVal: 4.845 ± 0.332
0.822ValTrp: 0.822 ± 0.126
2.667ValTyr: 2.667 ± 0.257
0.0ValXaa: 0.0 ± 0.0
Trp
0.911TrpAla: 0.911 ± 0.141
0.289TrpCys: 0.289 ± 0.078
1.222TrpAsp: 1.222 ± 0.196
1.0TrpGlu: 1.0 ± 0.123
0.489TrpPhe: 0.489 ± 0.111
0.733TrpGly: 0.733 ± 0.133
0.422TrpHis: 0.422 ± 0.099
0.956TrpIle: 0.956 ± 0.153
0.956TrpLys: 0.956 ± 0.136
1.311TrpLeu: 1.311 ± 0.197
0.467TrpMet: 0.467 ± 0.094
0.733TrpAsn: 0.733 ± 0.134
0.467TrpPro: 0.467 ± 0.087
0.556TrpGln: 0.556 ± 0.13
0.711TrpArg: 0.711 ± 0.127
0.911TrpSer: 0.911 ± 0.145
0.533TrpThr: 0.533 ± 0.108
0.822TrpVal: 0.822 ± 0.147
0.2TrpTrp: 0.2 ± 0.082
0.378TrpTyr: 0.378 ± 0.095
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.756TyrAla: 2.756 ± 0.246
0.378TyrCys: 0.378 ± 0.092
2.933TyrAsp: 2.933 ± 0.252
2.311TyrGlu: 2.311 ± 0.285
1.689TyrPhe: 1.689 ± 0.208
2.6TyrGly: 2.6 ± 0.22
0.956TyrHis: 0.956 ± 0.151
2.244TyrIle: 2.244 ± 0.254
2.778TyrLys: 2.778 ± 0.261
3.022TyrLeu: 3.022 ± 0.242
1.156TyrMet: 1.156 ± 0.148
1.756TyrAsn: 1.756 ± 0.181
1.178TyrPro: 1.178 ± 0.162
1.689TyrGln: 1.689 ± 0.202
2.422TyrArg: 2.422 ± 0.238
1.844TyrSer: 1.844 ± 0.21
2.4TyrThr: 2.4 ± 0.293
2.511TyrVal: 2.511 ± 0.226
0.511TyrTrp: 0.511 ± 0.102
1.422TyrTyr: 1.422 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 192 proteins (45000 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski