Thioclava indica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thioclava

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A074JT76|A0A074JT76_9RHOB Uncharacterized protein OS=Thioclava indica OX=1353528 GN=DT23_11805 PE=4 SV=1
MM1 pKa = 6.62TVYY4 pKa = 10.76YY5 pKa = 10.83VDD7 pKa = 3.71TNGNDD12 pKa = 3.32NANGSDD18 pKa = 3.52GSPFRR23 pKa = 11.84TIGQALNANLNPGDD37 pKa = 4.27EE38 pKa = 4.31IVVRR42 pKa = 11.84AGTYY46 pKa = 10.15NEE48 pKa = 4.56SLNINQGGSAAGDD61 pKa = 3.19ITIRR65 pKa = 11.84AEE67 pKa = 4.27DD68 pKa = 4.39PGTALIRR75 pKa = 11.84PPSGAWNAISVNANYY90 pKa = 8.52VTIEE94 pKa = 4.05GFDD97 pKa = 3.4IANAAGDD104 pKa = 4.22GIEE107 pKa = 4.42ANSVHH112 pKa = 7.04HH113 pKa = 6.05ITIADD118 pKa = 3.7NTVHH122 pKa = 6.71GNGEE126 pKa = 3.92SGIQFNYY133 pKa = 10.8SEE135 pKa = 5.39FITIEE140 pKa = 3.94GNEE143 pKa = 4.34TYY145 pKa = 11.16DD146 pKa = 3.52NASSGWYY153 pKa = 9.62SGISIYY159 pKa = 8.08QNRR162 pKa = 11.84NVSGDD167 pKa = 3.38TTTPGYY173 pKa = 9.03RR174 pKa = 11.84TIIRR178 pKa = 11.84DD179 pKa = 3.93NITHH183 pKa = 7.32DD184 pKa = 3.46NVTQSGAHH192 pKa = 5.79TDD194 pKa = 3.48GNGIIIDD201 pKa = 4.63DD202 pKa = 4.21FQSTQTDD209 pKa = 3.02GHH211 pKa = 6.63PNYY214 pKa = 9.89TYY216 pKa = 7.8PTLVEE221 pKa = 4.09NNVAYY226 pKa = 10.49GNGGKK231 pKa = 10.25GIQVTWSDD239 pKa = 4.09YY240 pKa = 8.98VTVSNNTAYY249 pKa = 10.6HH250 pKa = 6.09NNVDD254 pKa = 4.17LQNDD258 pKa = 4.25GTWRR262 pKa = 11.84GEE264 pKa = 3.59ISNAQSSNNTFVNNIAVADD283 pKa = 4.02PSINSNNTAIDD294 pKa = 3.52NNSYY298 pKa = 11.01GGYY301 pKa = 10.41SNDD304 pKa = 3.04NVVWEE309 pKa = 4.96DD310 pKa = 3.18NVLYY314 pKa = 10.54NGKK317 pKa = 7.58TGQMSASTDD326 pKa = 3.34GGNDD330 pKa = 3.22APSAADD336 pKa = 3.49GNLIGVNPGFVNPANGDD353 pKa = 3.53FSISAGSVAVNLGADD368 pKa = 3.62MDD370 pKa = 4.49GSSSVTSPPTTSTPDD385 pKa = 3.47PVVTPDD391 pKa = 4.04PDD393 pKa = 3.8PVVTPDD399 pKa = 3.97PAPTTPAVEE408 pKa = 3.99NAAPVAHH415 pKa = 7.47DD416 pKa = 5.23DD417 pKa = 4.05INFKK421 pKa = 10.09TSPNAPLILQQSQLLSNDD439 pKa = 2.81SDD441 pKa = 4.33ADD443 pKa = 3.48GDD445 pKa = 4.52ALILTNVVGSTQGSAKK461 pKa = 9.16LTSGNNISFTPEE473 pKa = 3.17SDD475 pKa = 3.36ASGTASVTYY484 pKa = 9.4TVSDD488 pKa = 3.66QNGGTDD494 pKa = 3.03TATAYY499 pKa = 9.98IDD501 pKa = 3.47IVAAAPSGPGGG512 pKa = 3.39

Molecular weight: 52.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A074K5E6|A0A074K5E6_9RHOB Uncharacterized protein OS=Thioclava indica OX=1353528 GN=DT23_17610 PE=3 SV=1
MM1 pKa = 7.26AAIVRR6 pKa = 11.84RR7 pKa = 11.84PPAMNTRR14 pKa = 11.84KK15 pKa = 10.33VIGGFPPVFRR25 pKa = 11.84PLTRR29 pKa = 11.84FAFHH33 pKa = 7.33ASRR36 pKa = 11.84FSRR39 pKa = 3.7

Molecular weight: 4.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3671

0

3671

1133096

29

6803

308.7

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.534 ± 0.059

0.846 ± 0.015

5.916 ± 0.039

5.515 ± 0.044

3.683 ± 0.033

8.624 ± 0.044

2.037 ± 0.022

5.364 ± 0.032

3.523 ± 0.033

10.135 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.84 ± 0.027

2.632 ± 0.031

5.107 ± 0.033

3.386 ± 0.024

6.417 ± 0.045

5.368 ± 0.047

5.376 ± 0.053

7.074 ± 0.033

1.398 ± 0.019

2.225 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski