Cyanoramphus nest associated circular K DNA virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7UZA8|L7UZA8_9VIRU Replication associated protein OS=Cyanoramphus nest associated circular K DNA virus OX=1282444 PE=4 SV=1
MM1 pKa = 6.62NHH3 pKa = 5.27ARR5 pKa = 11.84ARR7 pKa = 11.84SKK9 pKa = 10.14NWCFTSYY16 pKa = 11.25DD17 pKa = 3.51EE18 pKa = 5.71SEE20 pKa = 4.41PDD22 pKa = 3.26LARR25 pKa = 11.84HH26 pKa = 5.19QEE28 pKa = 3.68HH29 pKa = 6.04LTYY32 pKa = 10.63YY33 pKa = 9.92IYY35 pKa = 10.65QPEE38 pKa = 4.15RR39 pKa = 11.84GVQGRR44 pKa = 11.84RR45 pKa = 11.84HH46 pKa = 4.49FQGYY50 pKa = 9.48AEE52 pKa = 4.06SRR54 pKa = 11.84EE55 pKa = 4.14RR56 pKa = 11.84IGVRR60 pKa = 11.84EE61 pKa = 4.19LQSFIGSRR69 pKa = 11.84AHH71 pKa = 6.96CEE73 pKa = 3.46IAFDD77 pKa = 4.25PEE79 pKa = 3.86ASRR82 pKa = 11.84LYY84 pKa = 10.2CRR86 pKa = 11.84KK87 pKa = 9.57EE88 pKa = 3.77STRR91 pKa = 11.84DD92 pKa = 3.29GVTTEE97 pKa = 3.41WGLFRR102 pKa = 11.84GKK104 pKa = 10.14KK105 pKa = 8.95QGSRR109 pKa = 11.84TDD111 pKa = 3.06IGTACDD117 pKa = 3.58TIRR120 pKa = 11.84RR121 pKa = 11.84SGLGACIKK129 pKa = 8.53EE130 pKa = 4.16HH131 pKa = 7.15PEE133 pKa = 3.97TFVKK137 pKa = 10.42YY138 pKa = 10.26HH139 pKa = 6.62GGLTIYY145 pKa = 10.3AAQQFDD151 pKa = 3.88PRR153 pKa = 11.84RR154 pKa = 11.84CTDD157 pKa = 3.57DD158 pKa = 4.06PPDD161 pKa = 3.21VRR163 pKa = 11.84IYY165 pKa = 11.13YY166 pKa = 10.26GVTGIGKK173 pKa = 7.62TRR175 pKa = 11.84SVYY178 pKa = 10.22AQYY181 pKa = 11.67GNDD184 pKa = 3.91IYY186 pKa = 11.37TKK188 pKa = 10.73DD189 pKa = 4.52DD190 pKa = 3.88SKK192 pKa = 10.71WWNGYY197 pKa = 8.63AAQKK201 pKa = 10.6CILFDD206 pKa = 3.63DD207 pKa = 4.17WVGSEE212 pKa = 4.39EE213 pKa = 3.99ISPVSLLKK221 pKa = 10.51ICDD224 pKa = 3.75RR225 pKa = 11.84YY226 pKa = 9.96PLQVQTKK233 pKa = 8.74GGYY236 pKa = 9.12VPLARR241 pKa = 11.84TTTVIIFTTTQHH253 pKa = 5.41EE254 pKa = 4.41NFWYY258 pKa = 10.45GGTKK262 pKa = 10.04HH263 pKa = 5.51EE264 pKa = 5.57ANWCSQRR271 pKa = 11.84AAWDD275 pKa = 3.41RR276 pKa = 11.84RR277 pKa = 11.84VSEE280 pKa = 5.42RR281 pKa = 11.84RR282 pKa = 11.84TEE284 pKa = 3.83WTQPP288 pKa = 3.13

Molecular weight: 33.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7UZA8|L7UZA8_9VIRU Replication associated protein OS=Cyanoramphus nest associated circular K DNA virus OX=1282444 PE=4 SV=1
MM1 pKa = 7.77PRR3 pKa = 11.84RR4 pKa = 11.84TYY6 pKa = 10.5GGRR9 pKa = 11.84SVRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84GSVRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84APMRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VHH29 pKa = 6.11HH30 pKa = 5.88PRR32 pKa = 11.84IGRR35 pKa = 11.84GFSQYY40 pKa = 9.76VASVFKK46 pKa = 10.63TEE48 pKa = 4.15EE49 pKa = 3.74VSAGIVVAGGTSFSAFAPVSIVSMASAAASPANAVGYY86 pKa = 10.03AFQFRR91 pKa = 11.84IYY93 pKa = 10.29DD94 pKa = 3.42ISNPTSGVPSDD105 pKa = 4.09PSFITYY111 pKa = 9.35AQIYY115 pKa = 9.75DD116 pKa = 3.47QFFLTKK122 pKa = 9.09ATLVLRR128 pKa = 11.84PPLGIRR134 pKa = 11.84SFPQTVVTNAATGQIPVAQYY154 pKa = 10.54LPSTSRR160 pKa = 11.84HH161 pKa = 4.71LSYY164 pKa = 10.67IEE166 pKa = 4.4RR167 pKa = 11.84DD168 pKa = 3.16SSLALTQPLPANGDD182 pKa = 3.37GTFANRR188 pKa = 11.84ARR190 pKa = 11.84YY191 pKa = 8.21GAKK194 pKa = 9.1QHH196 pKa = 5.85SAYY199 pKa = 10.27GPIVRR204 pKa = 11.84TFWPRR209 pKa = 11.84YY210 pKa = 9.44LGAAYY215 pKa = 10.44NGADD219 pKa = 3.82YY220 pKa = 10.18TSSEE224 pKa = 4.28YY225 pKa = 11.15VIGNRR230 pKa = 11.84WLTQNRR236 pKa = 11.84SVAVGGYY243 pKa = 9.27IVLLFAYY250 pKa = 9.62NGYY253 pKa = 10.25GSAGGGPSTGAQPIINYY270 pKa = 9.5DD271 pKa = 3.58VEE273 pKa = 4.99VYY275 pKa = 7.45WHH277 pKa = 5.75VKK279 pKa = 9.12FRR281 pKa = 11.84YY282 pKa = 7.3TVYY285 pKa = 11.0GG286 pKa = 3.51

Molecular weight: 31.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

574

286

288

287.0

32.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.537 ± 1.683

1.568 ± 1.146

4.181 ± 1.268

4.181 ± 1.779

4.181 ± 0.522

9.059 ± 0.534

2.439 ± 0.505

5.401 ± 0.114

2.962 ± 1.143

4.355 ± 0.139

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.697 ± 0.257

2.787 ± 0.518

5.226 ± 1.036

4.53 ± 0.5

9.582 ± 0.103

7.666 ± 1.298

7.317 ± 0.748

6.62 ± 1.551

2.091 ± 0.762

6.62 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski