Amino acid dipepetide frequency for Lactobacillus florum 8D

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.485AlaAla: 8.485 ± 0.19
0.451AlaCys: 0.451 ± 0.038
4.953AlaAsp: 4.953 ± 0.137
4.437AlaGlu: 4.437 ± 0.132
2.983AlaPhe: 2.983 ± 0.102
6.237AlaGly: 6.237 ± 0.145
1.586AlaHis: 1.586 ± 0.07
5.429AlaIle: 5.429 ± 0.141
4.683AlaLys: 4.683 ± 0.115
8.069AlaLeu: 8.069 ± 0.144
2.002AlaMet: 2.002 ± 0.085
3.889AlaAsn: 3.889 ± 0.111
2.673AlaPro: 2.673 ± 0.083
4.464AlaGln: 4.464 ± 0.115
3.313AlaArg: 3.313 ± 0.097
4.315AlaSer: 4.315 ± 0.101
5.918AlaThr: 5.918 ± 0.132
6.337AlaVal: 6.337 ± 0.131
0.676AlaTrp: 0.676 ± 0.046
2.535AlaTyr: 2.535 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
0.313CysAla: 0.313 ± 0.027
0.065CysCys: 0.065 ± 0.015
0.292CysAsp: 0.292 ± 0.031
0.292CysGlu: 0.292 ± 0.033
0.219CysPhe: 0.219 ± 0.026
0.54CysGly: 0.54 ± 0.039
0.162CysHis: 0.162 ± 0.021
0.249CysIle: 0.249 ± 0.027
0.197CysLys: 0.197 ± 0.023
0.657CysLeu: 0.657 ± 0.048
0.07CysMet: 0.07 ± 0.014
0.197CysAsn: 0.197 ± 0.023
0.251CysPro: 0.251 ± 0.029
0.303CysGln: 0.303 ± 0.028
0.192CysArg: 0.192 ± 0.024
0.308CysSer: 0.308 ± 0.03
0.213CysThr: 0.213 ± 0.022
0.238CysVal: 0.238 ± 0.025
0.068CysTrp: 0.068 ± 0.013
0.227CysTyr: 0.227 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.824AspAla: 3.824 ± 0.106
0.303AspCys: 0.303 ± 0.025
3.286AspAsp: 3.286 ± 0.123
3.529AspGlu: 3.529 ± 0.109
2.8AspPhe: 2.8 ± 0.095
3.256AspGly: 3.256 ± 0.097
1.543AspHis: 1.543 ± 0.074
2.818AspIle: 2.818 ± 0.094
2.494AspLys: 2.494 ± 0.095
5.586AspLeu: 5.586 ± 0.125
1.116AspMet: 1.116 ± 0.053
2.297AspAsn: 2.297 ± 0.098
2.427AspPro: 2.427 ± 0.086
4.099AspGln: 4.099 ± 0.111
2.581AspArg: 2.581 ± 0.087
2.481AspSer: 2.481 ± 0.077
2.427AspThr: 2.427 ± 0.092
3.602AspVal: 3.602 ± 0.1
0.684AspTrp: 0.684 ± 0.047
2.286AspTyr: 2.286 ± 0.08
0.0AspXaa: 0.0 ± 0.0
Glu
3.872GluAla: 3.872 ± 0.113
0.178GluCys: 0.178 ± 0.02
1.916GluAsp: 1.916 ± 0.081
2.202GluGlu: 2.202 ± 0.089
1.967GluPhe: 1.967 ± 0.074
2.01GluGly: 2.01 ± 0.072
1.275GluHis: 1.275 ± 0.062
4.121GluIle: 4.121 ± 0.119
3.418GluLys: 3.418 ± 0.106
6.253GluLeu: 6.253 ± 0.159
1.54GluMet: 1.54 ± 0.062
2.602GluAsn: 2.602 ± 0.1
1.821GluPro: 1.821 ± 0.074
3.602GluGln: 3.602 ± 0.122
2.602GluArg: 2.602 ± 0.093
2.164GluSer: 2.164 ± 0.082
3.224GluThr: 3.224 ± 0.096
3.289GluVal: 3.289 ± 0.108
0.376GluTrp: 0.376 ± 0.035
1.489GluTyr: 1.489 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.321PheAla: 3.321 ± 0.109
0.268PheCys: 0.268 ± 0.025
2.502PheAsp: 2.502 ± 0.084
1.865PheGlu: 1.865 ± 0.089
1.978PhePhe: 1.978 ± 0.081
3.208PheGly: 3.208 ± 0.102
0.813PheHis: 0.813 ± 0.045
2.697PheIle: 2.697 ± 0.108
2.2PheLys: 2.2 ± 0.09
4.332PheLeu: 4.332 ± 0.155
0.905PheMet: 0.905 ± 0.051
2.156PheAsn: 2.156 ± 0.08
1.689PhePro: 1.689 ± 0.078
2.01PheGln: 2.01 ± 0.057
1.419PheArg: 1.419 ± 0.06
3.086PheSer: 3.086 ± 0.094
2.394PheThr: 2.394 ± 0.084
2.978PheVal: 2.978 ± 0.105
0.559PheTrp: 0.559 ± 0.045
1.673PheTyr: 1.673 ± 0.065
0.0PheXaa: 0.0 ± 0.0
Gly
4.896GlyAla: 4.896 ± 0.121
0.386GlyCys: 0.386 ± 0.034
3.164GlyAsp: 3.164 ± 0.103
2.859GlyGlu: 2.859 ± 0.104
3.108GlyPhe: 3.108 ± 0.088
4.259GlyGly: 4.259 ± 0.135
1.438GlyHis: 1.438 ± 0.07
5.534GlyIle: 5.534 ± 0.138
4.288GlyLys: 4.288 ± 0.111
6.812GlyLeu: 6.812 ± 0.158
1.951GlyMet: 1.951 ± 0.072
2.843GlyAsn: 2.843 ± 0.101
1.694GlyPro: 1.694 ± 0.065
3.032GlyGln: 3.032 ± 0.098
2.77GlyArg: 2.77 ± 0.101
4.002GlySer: 4.002 ± 0.112
4.234GlyThr: 4.234 ± 0.12
4.699GlyVal: 4.699 ± 0.138
0.767GlyTrp: 0.767 ± 0.049
2.537GlyTyr: 2.537 ± 0.079
0.0GlyXaa: 0.0 ± 0.0
His
1.465HisAla: 1.465 ± 0.067
0.162HisCys: 0.162 ± 0.025
1.457HisAsp: 1.457 ± 0.06
1.265HisGlu: 1.265 ± 0.059
1.016HisPhe: 1.016 ± 0.051
1.81HisGly: 1.81 ± 0.073
0.77HisHis: 0.77 ± 0.044
1.0HisIle: 1.0 ± 0.056
0.83HisLys: 0.83 ± 0.048
2.264HisLeu: 2.264 ± 0.076
0.4HisMet: 0.4 ± 0.032
0.981HisAsn: 0.981 ± 0.053
1.281HisPro: 1.281 ± 0.06
1.754HisGln: 1.754 ± 0.077
1.197HisArg: 1.197 ± 0.065
1.105HisSer: 1.105 ± 0.054
0.892HisThr: 0.892 ± 0.049
1.319HisVal: 1.319 ± 0.06
0.319HisTrp: 0.319 ± 0.028
0.924HisTyr: 0.924 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
5.713IleAla: 5.713 ± 0.144
0.476IleCys: 0.476 ± 0.039
4.097IleAsp: 4.097 ± 0.114
3.408IleGlu: 3.408 ± 0.096
2.999IlePhe: 2.999 ± 0.113
4.856IleGly: 4.856 ± 0.135
1.146IleHis: 1.146 ± 0.058
5.204IleIle: 5.204 ± 0.155
4.626IleLys: 4.626 ± 0.13
6.11IleLeu: 6.11 ± 0.161
1.756IleMet: 1.756 ± 0.077
3.721IleAsn: 3.721 ± 0.107
2.897IlePro: 2.897 ± 0.088
2.602IleGln: 2.602 ± 0.092
2.518IleArg: 2.518 ± 0.088
4.734IleSer: 4.734 ± 0.139
4.286IleThr: 4.286 ± 0.12
4.788IleVal: 4.788 ± 0.127
0.54IleTrp: 0.54 ± 0.046
2.151IleTyr: 2.151 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
3.832LysAla: 3.832 ± 0.11
0.159LysCys: 0.159 ± 0.025
2.491LysAsp: 2.491 ± 0.094
2.77LysGlu: 2.77 ± 0.089
1.956LysPhe: 1.956 ± 0.066
2.632LysGly: 2.632 ± 0.101
1.246LysHis: 1.246 ± 0.053
4.478LysIle: 4.478 ± 0.114
4.099LysLys: 4.099 ± 0.119
6.275LysLeu: 6.275 ± 0.135
1.821LysMet: 1.821 ± 0.058
3.351LysAsn: 3.351 ± 0.097
2.259LysPro: 2.259 ± 0.077
4.032LysGln: 4.032 ± 0.117
3.062LysArg: 3.062 ± 0.103
2.983LysSer: 2.983 ± 0.098
4.075LysThr: 4.075 ± 0.102
3.356LysVal: 3.356 ± 0.091
0.505LysTrp: 0.505 ± 0.038
2.119LysTyr: 2.119 ± 0.086
0.0LysXaa: 0.0 ± 0.0
Leu
9.798LeuAla: 9.798 ± 0.192
0.524LeuCys: 0.524 ± 0.038
5.45LeuAsp: 5.45 ± 0.12
4.807LeuGlu: 4.807 ± 0.127
4.218LeuPhe: 4.218 ± 0.14
6.934LeuGly: 6.934 ± 0.171
2.151LeuHis: 2.151 ± 0.08
7.239LeuIle: 7.239 ± 0.195
5.623LeuLys: 5.623 ± 0.127
10.631LeuLeu: 10.631 ± 0.273
2.354LeuMet: 2.354 ± 0.09
4.818LeuAsn: 4.818 ± 0.107
4.642LeuPro: 4.642 ± 0.113
6.207LeuGln: 6.207 ± 0.169
4.305LeuArg: 4.305 ± 0.116
6.196LeuSer: 6.196 ± 0.148
6.961LeuThr: 6.961 ± 0.149
7.547LeuVal: 7.547 ± 0.155
0.835LeuTrp: 0.835 ± 0.051
2.681LeuTyr: 2.681 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
2.075MetAla: 2.075 ± 0.078
0.089MetCys: 0.089 ± 0.015
1.205MetAsp: 1.205 ± 0.055
1.213MetGlu: 1.213 ± 0.057
0.8MetPhe: 0.8 ± 0.051
1.64MetGly: 1.64 ± 0.064
0.376MetHis: 0.376 ± 0.031
1.81MetIle: 1.81 ± 0.069
1.692MetLys: 1.692 ± 0.062
2.337MetLeu: 2.337 ± 0.071
0.654MetMet: 0.654 ± 0.044
1.24MetAsn: 1.24 ± 0.059
0.965MetPro: 0.965 ± 0.049
1.316MetGln: 1.316 ± 0.062
0.986MetArg: 0.986 ± 0.044
1.351MetSer: 1.351 ± 0.062
1.594MetThr: 1.594 ± 0.066
1.886MetVal: 1.886 ± 0.069
0.168MetTrp: 0.168 ± 0.024
0.573MetTyr: 0.573 ± 0.045
0.0MetXaa: 0.0 ± 0.0
Asn
3.116AsnAla: 3.116 ± 0.095
0.232AsnCys: 0.232 ± 0.026
2.667AsnAsp: 2.667 ± 0.098
2.537AsnGlu: 2.537 ± 0.091
2.032AsnPhe: 2.032 ± 0.084
3.202AsnGly: 3.202 ± 0.084
1.248AsnHis: 1.248 ± 0.064
2.662AsnIle: 2.662 ± 0.092
2.44AsnLys: 2.44 ± 0.094
4.651AsnLeu: 4.651 ± 0.117
0.992AsnMet: 0.992 ± 0.052
2.589AsnAsn: 2.589 ± 0.113
2.645AsnPro: 2.645 ± 0.088
3.986AsnGln: 3.986 ± 0.108
2.432AsnArg: 2.432 ± 0.087
2.518AsnSer: 2.518 ± 0.092
2.183AsnThr: 2.183 ± 0.087
3.043AsnVal: 3.043 ± 0.094
0.667AsnTrp: 0.667 ± 0.05
1.981AsnTyr: 1.981 ± 0.078
0.0AsnXaa: 0.0 ± 0.0
Pro
3.845ProAla: 3.845 ± 0.126
0.116ProCys: 0.116 ± 0.017
2.321ProAsp: 2.321 ± 0.076
2.605ProGlu: 2.605 ± 0.086
1.665ProPhe: 1.665 ± 0.072
2.508ProGly: 2.508 ± 0.084
0.84ProHis: 0.84 ± 0.044
2.602ProIle: 2.602 ± 0.091
2.251ProLys: 2.251 ± 0.076
3.87ProLeu: 3.87 ± 0.112
0.759ProMet: 0.759 ± 0.043
1.859ProAsn: 1.859 ± 0.073
0.824ProPro: 0.824 ± 0.05
2.227ProGln: 2.227 ± 0.093
1.373ProArg: 1.373 ± 0.066
2.048ProSer: 2.048 ± 0.083
2.94ProThr: 2.94 ± 0.097
3.405ProVal: 3.405 ± 0.093
0.351ProTrp: 0.351 ± 0.031
1.259ProTyr: 1.259 ± 0.061
0.0ProXaa: 0.0 ± 0.0
Gln
5.691GlnAla: 5.691 ± 0.141
0.17GlnCys: 0.17 ± 0.025
2.232GlnAsp: 2.232 ± 0.082
2.57GlnGlu: 2.57 ± 0.092
2.273GlnPhe: 2.273 ± 0.082
2.856GlnGly: 2.856 ± 0.086
1.746GlnHis: 1.746 ± 0.07
3.875GlnIle: 3.875 ± 0.094
3.432GlnLys: 3.432 ± 0.107
7.41GlnLeu: 7.41 ± 0.196
1.197GlnMet: 1.197 ± 0.059
2.348GlnAsn: 2.348 ± 0.089
2.759GlnPro: 2.759 ± 0.097
5.669GlnGln: 5.669 ± 0.24
3.289GlnArg: 3.289 ± 0.109
2.781GlnSer: 2.781 ± 0.108
4.143GlnThr: 4.143 ± 0.113
4.388GlnVal: 4.388 ± 0.123
0.454GlnTrp: 0.454 ± 0.032
1.608GlnTyr: 1.608 ± 0.067
0.0GlnXaa: 0.0 ± 0.0
Arg
3.432ArgAla: 3.432 ± 0.107
0.192ArgCys: 0.192 ± 0.027
2.197ArgAsp: 2.197 ± 0.074
2.254ArgGlu: 2.254 ± 0.076
1.986ArgPhe: 1.986 ± 0.069
2.618ArgGly: 2.618 ± 0.087
1.065ArgHis: 1.065 ± 0.058
2.964ArgIle: 2.964 ± 0.1
2.581ArgLys: 2.581 ± 0.086
4.915ArgLeu: 4.915 ± 0.136
1.159ArgMet: 1.159 ± 0.05
1.937ArgAsn: 1.937 ± 0.069
1.705ArgPro: 1.705 ± 0.073
2.87ArgGln: 2.87 ± 0.105
2.416ArgArg: 2.416 ± 0.098
2.508ArgSer: 2.508 ± 0.077
2.424ArgThr: 2.424 ± 0.079
2.916ArgVal: 2.916 ± 0.099
0.43ArgTrp: 0.43 ± 0.04
1.654ArgTyr: 1.654 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
4.286SerAla: 4.286 ± 0.113
0.284SerCys: 0.284 ± 0.027
3.189SerAsp: 3.189 ± 0.096
3.143SerGlu: 3.143 ± 0.087
2.775SerPhe: 2.775 ± 0.083
4.099SerGly: 4.099 ± 0.11
1.138SerHis: 1.138 ± 0.056
3.61SerIle: 3.61 ± 0.122
3.297SerLys: 3.297 ± 0.111
6.05SerLeu: 6.05 ± 0.129
1.384SerMet: 1.384 ± 0.068
2.697SerAsn: 2.697 ± 0.09
1.881SerPro: 1.881 ± 0.077
3.218SerGln: 3.218 ± 0.098
2.278SerArg: 2.278 ± 0.076
3.41SerSer: 3.41 ± 0.107
3.137SerThr: 3.137 ± 0.096
3.713SerVal: 3.713 ± 0.097
0.589SerTrp: 0.589 ± 0.041
2.119SerTyr: 2.119 ± 0.083
0.0SerXaa: 0.0 ± 0.0
Thr
5.95ThrAla: 5.95 ± 0.118
0.276ThrCys: 0.276 ± 0.027
3.756ThrAsp: 3.756 ± 0.121
3.11ThrGlu: 3.11 ± 0.103
2.386ThrPhe: 2.386 ± 0.083
4.794ThrGly: 4.794 ± 0.12
1.065ThrHis: 1.065 ± 0.052
4.74ThrIle: 4.74 ± 0.109
3.967ThrLys: 3.967 ± 0.099
5.229ThrLeu: 5.229 ± 0.112
1.454ThrMet: 1.454 ± 0.07
3.356ThrAsn: 3.356 ± 0.093
2.721ThrPro: 2.721 ± 0.093
2.394ThrGln: 2.394 ± 0.089
2.219ThrArg: 2.219 ± 0.073
3.702ThrSer: 3.702 ± 0.112
4.283ThrThr: 4.283 ± 0.119
4.513ThrVal: 4.513 ± 0.113
0.551ThrTrp: 0.551 ± 0.038
1.897ThrTyr: 1.897 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
6.72ValAla: 6.72 ± 0.161
0.449ValCys: 0.449 ± 0.032
3.834ValAsp: 3.834 ± 0.125
3.086ValGlu: 3.086 ± 0.096
2.589ValPhe: 2.589 ± 0.08
5.029ValGly: 5.029 ± 0.138
1.354ValHis: 1.354 ± 0.069
5.313ValIle: 5.313 ± 0.136
3.748ValLys: 3.748 ± 0.107
6.774ValLeu: 6.774 ± 0.152
1.646ValMet: 1.646 ± 0.069
3.121ValAsn: 3.121 ± 0.099
2.924ValPro: 2.924 ± 0.088
3.235ValGln: 3.235 ± 0.092
2.862ValArg: 2.862 ± 0.079
4.229ValSer: 4.229 ± 0.128
5.037ValThr: 5.037 ± 0.132
5.658ValVal: 5.658 ± 0.129
0.546ValTrp: 0.546 ± 0.041
2.102ValTyr: 2.102 ± 0.078
0.0ValXaa: 0.0 ± 0.0
Trp
0.495TrpAla: 0.495 ± 0.035
0.043TrpCys: 0.043 ± 0.011
0.47TrpAsp: 0.47 ± 0.04
0.435TrpGlu: 0.435 ± 0.036
0.538TrpPhe: 0.538 ± 0.042
0.592TrpGly: 0.592 ± 0.04
0.232TrpHis: 0.232 ± 0.027
0.713TrpIle: 0.713 ± 0.049
0.511TrpLys: 0.511 ± 0.036
1.392TrpLeu: 1.392 ± 0.062
0.265TrpMet: 0.265 ± 0.024
0.47TrpAsn: 0.47 ± 0.032
0.259TrpPro: 0.259 ± 0.027
0.673TrpGln: 0.673 ± 0.045
0.473TrpArg: 0.473 ± 0.038
0.638TrpSer: 0.638 ± 0.042
0.397TrpThr: 0.397 ± 0.034
0.508TrpVal: 0.508 ± 0.04
0.149TrpTrp: 0.149 ± 0.022
0.386TrpTyr: 0.386 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.305TyrAla: 2.305 ± 0.078
0.23TyrCys: 0.23 ± 0.028
1.956TyrAsp: 1.956 ± 0.078
1.538TyrGlu: 1.538 ± 0.059
1.765TyrPhe: 1.765 ± 0.075
2.194TyrGly: 2.194 ± 0.073
1.016TyrHis: 1.016 ± 0.055
1.57TyrIle: 1.57 ± 0.064
1.124TyrLys: 1.124 ± 0.064
4.307TyrLeu: 4.307 ± 0.117
0.543TyrMet: 0.543 ± 0.042
1.351TyrAsn: 1.351 ± 0.062
1.351TyrPro: 1.351 ± 0.064
3.197TyrGln: 3.197 ± 0.108
1.937TyrArg: 1.937 ± 0.081
1.735TyrSer: 1.735 ± 0.081
1.6TyrThr: 1.6 ± 0.063
2.075TyrVal: 2.075 ± 0.073
0.405TyrTrp: 0.405 ± 0.034
1.238TyrTyr: 1.238 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1228 proteins (370066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski