Amino acid dipepetide frequency for marine bacterium AO1-C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.235AlaAla: 4.235 ± 0.054	0.617AlaCys: 0.617 ± 0.017	3.176AlaAsp: 3.176 ± 0.034	3.661AlaGlu: 3.661 ± 0.04	3.469AlaPhe: 3.469 ± 0.034	4.387AlaGly: 4.387 ± 0.053	1.348AlaHis: 1.348 ± 0.021	4.87AlaIle: 4.87 ± 0.042	4.97AlaLys: 4.97 ± 0.054	7.172AlaLeu: 7.172 ± 0.066	1.446AlaMet: 1.446 ± 0.024	3.877AlaAsn: 3.877 ± 0.041	2.195AlaPro: 2.195 ± 0.029	4.02AlaGln: 4.02 ± 0.039	2.599AlaArg: 2.599 ± 0.031	4.39AlaSer: 4.39 ± 0.041	4.195AlaThr: 4.195 ± 0.055	3.591AlaVal: 3.591 ± 0.037	0.757AlaTrp: 0.757 ± 0.017	2.949AlaTyr: 2.949 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.443CysAla: 0.443 ± 0.012	0.097CysCys: 0.097 ± 0.006	0.344CysAsp: 0.344 ± 0.011	0.391CysGlu: 0.391 ± 0.01	0.415CysPhe: 0.415 ± 0.011	0.512CysGly: 0.512 ± 0.015	0.167CysHis: 0.167 ± 0.008	0.495CysIle: 0.495 ± 0.012	0.44CysLys: 0.44 ± 0.011	0.748CysLeu: 0.748 ± 0.016	0.12CysMet: 0.12 ± 0.006	0.354CysAsn: 0.354 ± 0.012	0.281CysPro: 0.281 ± 0.011	0.311CysGln: 0.311 ± 0.01	0.268CysArg: 0.268 ± 0.01	0.43CysSer: 0.43 ± 0.013	0.46CysThr: 0.46 ± 0.013	0.43CysVal: 0.43 ± 0.011	0.097CysTrp: 0.097 ± 0.006	0.305CysTyr: 0.305 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	3.439AspAla: 3.439 ± 0.041	0.401AspCys: 0.401 ± 0.012	2.363AspAsp: 2.363 ± 0.032	3.043AspGlu: 3.043 ± 0.039	3.134AspPhe: 3.134 ± 0.033	3.373AspGly: 3.373 ± 0.051	0.886AspHis: 0.886 ± 0.017	3.607AspIle: 3.607 ± 0.034	3.685AspLys: 3.685 ± 0.043	5.043AspLeu: 5.043 ± 0.043	1.065AspMet: 1.065 ± 0.019	2.654AspAsn: 2.654 ± 0.033	1.648AspPro: 1.648 ± 0.025	2.307AspGln: 2.307 ± 0.031	1.88AspArg: 1.88 ± 0.024	2.659AspSer: 2.659 ± 0.029	2.56AspThr: 2.56 ± 0.032	2.859AspVal: 2.859 ± 0.03	0.735AspTrp: 0.735 ± 0.015	2.374AspTyr: 2.374 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	4.607GluAla: 4.607 ± 0.048	0.325GluCys: 0.325 ± 0.01	2.905GluAsp: 2.905 ± 0.039	4.271GluGlu: 4.271 ± 0.059	2.387GluPhe: 2.387 ± 0.031	3.531GluGly: 3.531 ± 0.038	1.112GluHis: 1.112 ± 0.018	4.443GluIle: 4.443 ± 0.044	5.111GluLys: 5.111 ± 0.055	5.716GluLeu: 5.716 ± 0.056	1.451GluMet: 1.451 ± 0.02	3.324GluAsn: 3.324 ± 0.039	1.42GluPro: 1.42 ± 0.021	2.969GluGln: 2.969 ± 0.036	2.392GluArg: 2.392 ± 0.033	2.886GluSer: 2.886 ± 0.031	2.89GluThr: 2.89 ± 0.035	4.379GluVal: 4.379 ± 0.041	0.671GluTrp: 0.671 ± 0.013	2.174GluTyr: 2.174 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.265PheAla: 3.265 ± 0.033	0.42PheCys: 0.42 ± 0.012	2.751PheAsp: 2.751 ± 0.033	2.968PheGlu: 2.968 ± 0.033	2.594PhePhe: 2.594 ± 0.031	3.123PheGly: 3.123 ± 0.04	0.87PheHis: 0.87 ± 0.018	3.278PheIle: 3.278 ± 0.036	3.402PheLys: 3.402 ± 0.037	4.483PheLeu: 4.483 ± 0.048	1.085PheMet: 1.085 ± 0.02	2.657PheAsn: 2.657 ± 0.033	1.698PhePro: 1.698 ± 0.022	1.986PheGln: 1.986 ± 0.027	2.023PheArg: 2.023 ± 0.023	3.495PheSer: 3.495 ± 0.035	3.23PheThr: 3.23 ± 0.041	2.96PheVal: 2.96 ± 0.033	0.688PheTrp: 0.688 ± 0.014	2.217PheTyr: 2.217 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	4.002GlyAla: 4.002 ± 0.046	0.549GlyCys: 0.549 ± 0.017	3.007GlyAsp: 3.007 ± 0.037	3.279GlyGlu: 3.279 ± 0.031	3.412GlyPhe: 3.412 ± 0.039	4.599GlyGly: 4.599 ± 0.074	1.171GlyHis: 1.171 ± 0.02	4.557GlyIle: 4.557 ± 0.039	5.242GlyLys: 5.242 ± 0.058	5.877GlyLeu: 5.877 ± 0.052	1.464GlyMet: 1.464 ± 0.021	3.811GlyAsn: 3.811 ± 0.05	1.111GlyPro: 1.111 ± 0.02	2.725GlyGln: 2.725 ± 0.035	2.3GlyArg: 2.3 ± 0.029	3.783GlySer: 3.783 ± 0.049	3.988GlyThr: 3.988 ± 0.065	4.347GlyVal: 4.347 ± 0.044	0.879GlyTrp: 0.879 ± 0.018	2.763GlyTyr: 2.763 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	0.914HisAla: 0.914 ± 0.016	0.193HisCys: 0.193 ± 0.008	0.708HisAsp: 0.708 ± 0.016	0.958HisGlu: 0.958 ± 0.017	1.226HisPhe: 1.226 ± 0.023	0.966HisGly: 0.966 ± 0.019	0.613HisHis: 0.613 ± 0.017	1.346HisIle: 1.346 ± 0.02	1.289HisLys: 1.289 ± 0.023	2.443HisLeu: 2.443 ± 0.031	0.388HisMet: 0.388 ± 0.013	0.987HisAsn: 0.987 ± 0.018	1.041HisPro: 1.041 ± 0.022	1.299HisGln: 1.299 ± 0.021	0.912HisArg: 0.912 ± 0.02	1.145HisSer: 1.145 ± 0.017	1.138HisThr: 1.138 ± 0.019	0.865HisVal: 0.865 ± 0.018	0.346HisTrp: 0.346 ± 0.01	1.036HisTyr: 1.036 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.21IleAla: 5.21 ± 0.049	0.542IleCys: 0.542 ± 0.015	3.999IleAsp: 3.999 ± 0.037	4.288IleGlu: 4.288 ± 0.039	3.027IlePhe: 3.027 ± 0.034	4.47IleGly: 4.47 ± 0.048	1.38IleHis: 1.38 ± 0.024	4.426IleIle: 4.426 ± 0.046	5.013IleLys: 5.013 ± 0.043	5.998IleLeu: 5.998 ± 0.054	1.117IleMet: 1.117 ± 0.021	3.903IleAsn: 3.903 ± 0.038	2.855IlePro: 2.855 ± 0.032	3.254IleGln: 3.254 ± 0.037	2.94IleArg: 2.94 ± 0.035	4.454IleSer: 4.454 ± 0.049	4.647IleThr: 4.647 ± 0.043	4.038IleVal: 4.038 ± 0.039	0.768IleTrp: 0.768 ± 0.018	2.63IleTyr: 2.63 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	5.659LysAla: 5.659 ± 0.068	0.304LysCys: 0.304 ± 0.01	3.915LysAsp: 3.915 ± 0.04	4.994LysGlu: 4.994 ± 0.05	2.801LysPhe: 2.801 ± 0.031	4.513LysGly: 4.513 ± 0.045	1.506LysHis: 1.506 ± 0.025	5.47LysIle: 5.47 ± 0.044	7.041LysLys: 7.041 ± 0.071	6.882LysLeu: 6.882 ± 0.065	2.023LysMet: 2.023 ± 0.026	4.585LysAsn: 4.585 ± 0.04	2.687LysPro: 2.687 ± 0.035	3.65LysGln: 3.65 ± 0.042	3.252LysArg: 3.252 ± 0.035	4.183LysSer: 4.183 ± 0.043	4.395LysThr: 4.395 ± 0.041	5.165LysVal: 5.165 ± 0.045	0.761LysTrp: 0.761 ± 0.016	3.057LysTyr: 3.057 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	6.862LeuAla: 6.862 ± 0.054	0.705LeuCys: 0.705 ± 0.015	4.987LeuAsp: 4.987 ± 0.042	6.252LeuGlu: 6.252 ± 0.068	4.458LeuPhe: 4.458 ± 0.046	6.009LeuGly: 6.009 ± 0.053	1.912LeuHis: 1.912 ± 0.029	6.271LeuIle: 6.271 ± 0.058	8.142LeuLys: 8.142 ± 0.061	9.6LeuLeu: 9.6 ± 0.072	2.149LeuMet: 2.149 ± 0.026	5.425LeuAsn: 5.425 ± 0.047	4.43LeuPro: 4.43 ± 0.053	4.657LeuGln: 4.657 ± 0.049	4.099LeuArg: 4.099 ± 0.039	6.669LeuSer: 6.669 ± 0.053	5.694LeuThr: 5.694 ± 0.047	5.762LeuVal: 5.762 ± 0.043	1.12LeuTrp: 1.12 ± 0.024	3.508LeuTyr: 3.508 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	1.58MetAla: 1.58 ± 0.026	0.121MetCys: 0.121 ± 0.006	1.027MetAsp: 1.027 ± 0.019	1.162MetGlu: 1.162 ± 0.021	0.865MetPhe: 0.865 ± 0.017	1.391MetGly: 1.391 ± 0.024	0.454MetHis: 0.454 ± 0.012	1.411MetIle: 1.411 ± 0.023	1.926MetLys: 1.926 ± 0.028	2.026MetLeu: 2.026 ± 0.029	0.591MetMet: 0.591 ± 0.014	1.227MetAsn: 1.227 ± 0.023	0.855MetPro: 0.855 ± 0.018	1.025MetGln: 1.025 ± 0.017	0.962MetArg: 0.962 ± 0.018	1.316MetSer: 1.316 ± 0.022	1.104MetThr: 1.104 ± 0.02	1.375MetVal: 1.375 ± 0.024	0.184MetTrp: 0.184 ± 0.008	0.681MetTyr: 0.681 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.742AsnAla: 3.742 ± 0.042	0.377AsnCys: 0.377 ± 0.011	2.724AsnAsp: 2.724 ± 0.028	3.115AsnGlu: 3.115 ± 0.034	2.628AsnPhe: 2.628 ± 0.035	3.657AsnGly: 3.657 ± 0.053	1.18AsnHis: 1.18 ± 0.02	4.009AsnIle: 4.009 ± 0.036	3.711AsnLys: 3.711 ± 0.042	5.718AsnLeu: 5.718 ± 0.049	1.007AsnMet: 1.007 ± 0.016	3.509AsnAsn: 3.509 ± 0.05	2.775AsnPro: 2.775 ± 0.032	3.267AsnGln: 3.267 ± 0.04	2.404AsnArg: 2.404 ± 0.028	3.055AsnSer: 3.055 ± 0.033	3.696AsnThr: 3.696 ± 0.041	2.983AsnVal: 2.983 ± 0.034	0.714AsnTrp: 0.714 ± 0.016	2.556AsnTyr: 2.556 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	2.485ProAla: 2.485 ± 0.032	0.208ProCys: 0.208 ± 0.009	2.106ProAsp: 2.106 ± 0.029	2.652ProGlu: 2.652 ± 0.034	1.683ProPhe: 1.683 ± 0.024	2.056ProGly: 2.056 ± 0.031	0.79ProHis: 0.79 ± 0.015	2.4ProIle: 2.4 ± 0.027	2.809ProLys: 2.809 ± 0.036	3.285ProLeu: 3.285 ± 0.034	0.703ProMet: 0.703 ± 0.016	2.22ProAsn: 2.22 ± 0.034	1.221ProPro: 1.221 ± 0.024	1.85ProGln: 1.85 ± 0.023	1.233ProArg: 1.233 ± 0.021	2.211ProSer: 2.211 ± 0.03	2.305ProThr: 2.305 ± 0.036	2.308ProVal: 2.308 ± 0.031	0.381ProTrp: 0.381 ± 0.012	1.377ProTyr: 1.377 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	3.87GlnAla: 3.87 ± 0.038	0.222GlnCys: 0.222 ± 0.009	2.185GlnAsp: 2.185 ± 0.023	3.159GlnGlu: 3.159 ± 0.042	1.97GlnPhe: 1.97 ± 0.025	2.882GlnGly: 2.882 ± 0.034	0.983GlnHis: 0.983 ± 0.018	3.212GlnIle: 3.212 ± 0.032	4.388GlnLys: 4.388 ± 0.05	5.109GlnLeu: 5.109 ± 0.06	1.173GlnMet: 1.173 ± 0.019	2.891GlnAsn: 2.891 ± 0.035	1.546GlnPro: 1.546 ± 0.021	3.135GlnGln: 3.135 ± 0.042	2.125GlnArg: 2.125 ± 0.027	2.683GlnSer: 2.683 ± 0.033	2.769GlnThr: 2.769 ± 0.028	3.297GlnVal: 3.297 ± 0.032	0.64GlnTrp: 0.64 ± 0.013	1.84GlnTyr: 1.84 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	2.344ArgAla: 2.344 ± 0.032	0.235ArgCys: 0.235 ± 0.009	1.937ArgAsp: 1.937 ± 0.027	2.296ArgGlu: 2.296 ± 0.028	2.346ArgPhe: 2.346 ± 0.031	2.211ArgGly: 2.211 ± 0.037	0.764ArgHis: 0.764 ± 0.014	2.883ArgIle: 2.883 ± 0.026	3.54ArgLys: 3.54 ± 0.038	4.39ArgLeu: 4.39 ± 0.036	0.967ArgMet: 0.967 ± 0.019	2.258ArgAsn: 2.258 ± 0.027	1.318ArgPro: 1.318 ± 0.02	1.955ArgGln: 1.955 ± 0.028	1.956ArgArg: 1.956 ± 0.03	2.2ArgSer: 2.2 ± 0.028	2.03ArgThr: 2.03 ± 0.026	2.606ArgVal: 2.606 ± 0.03	0.582ArgTrp: 0.582 ± 0.016	1.885ArgTyr: 1.885 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	3.693SerAla: 3.693 ± 0.042	0.466SerCys: 0.466 ± 0.012	2.859SerAsp: 2.859 ± 0.029	3.116SerGlu: 3.116 ± 0.039	3.57SerPhe: 3.57 ± 0.034	4.288SerGly: 4.288 ± 0.05	1.054SerHis: 1.054 ± 0.018	4.399SerIle: 4.399 ± 0.035	4.166SerLys: 4.166 ± 0.041	6.476SerLeu: 6.476 ± 0.05	1.193SerMet: 1.193 ± 0.017	3.441SerAsn: 3.441 ± 0.041	2.344SerPro: 2.344 ± 0.031	2.781SerGln: 2.781 ± 0.031	2.344SerArg: 2.344 ± 0.028	4.041SerSer: 4.041 ± 0.044	3.58SerThr: 3.58 ± 0.049	3.493SerVal: 3.493 ± 0.034	0.783SerTrp: 0.783 ± 0.018	2.424SerTyr: 2.424 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	3.747ThrAla: 3.747 ± 0.049	0.372ThrCys: 0.372 ± 0.012	2.866ThrAsp: 2.866 ± 0.036	2.965ThrGlu: 2.965 ± 0.03	3.039ThrPhe: 3.039 ± 0.036	3.998ThrGly: 3.998 ± 0.063	1.288ThrHis: 1.288 ± 0.021	4.317ThrIle: 4.317 ± 0.046	3.87ThrLys: 3.87 ± 0.038	6.06ThrLeu: 6.06 ± 0.049	0.945ThrMet: 0.945 ± 0.018	3.429ThrAsn: 3.429 ± 0.045	2.876ThrPro: 2.876 ± 0.039	3.182ThrGln: 3.182 ± 0.042	2.121ThrArg: 2.121 ± 0.027	3.862ThrSer: 3.862 ± 0.042	3.98ThrThr: 3.98 ± 0.059	3.284ThrVal: 3.284 ± 0.044	0.705ThrTrp: 0.705 ± 0.016	2.493ThrTyr: 2.493 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	4.432ValAla: 4.432 ± 0.044	0.49ValCys: 0.49 ± 0.013	3.108ValAsp: 3.108 ± 0.036	3.39ValGlu: 3.39 ± 0.038	3.183ValPhe: 3.183 ± 0.033	3.799ValGly: 3.799 ± 0.038	1.113ValHis: 1.113 ± 0.019	4.256ValIle: 4.256 ± 0.037	4.443ValLys: 4.443 ± 0.032	5.966ValLeu: 5.966 ± 0.049	1.366ValMet: 1.366 ± 0.021	3.349ValAsn: 3.349 ± 0.037	2.21ValPro: 2.21 ± 0.028	2.438ValGln: 2.438 ± 0.027	2.258ValArg: 2.258 ± 0.027	4.038ValSer: 4.038 ± 0.038	3.688ValThr: 3.688 ± 0.051	4.243ValVal: 4.243 ± 0.047	0.731ValTrp: 0.731 ± 0.017	2.522ValTyr: 2.522 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	0.773TrpAla: 0.773 ± 0.016	0.104TrpCys: 0.104 ± 0.005	0.597TrpAsp: 0.597 ± 0.016	0.702TrpGlu: 0.702 ± 0.015	0.627TrpPhe: 0.627 ± 0.015	0.824TrpGly: 0.824 ± 0.018	0.262TrpHis: 0.262 ± 0.008	0.831TrpIle: 0.831 ± 0.017	0.905TrpLys: 0.905 ± 0.017	1.328TrpLeu: 1.328 ± 0.022	0.329TrpMet: 0.329 ± 0.01	0.663TrpAsn: 0.663 ± 0.016	0.213TrpPro: 0.213 ± 0.009	0.761TrpGln: 0.761 ± 0.016	0.552TrpArg: 0.552 ± 0.014	0.694TrpSer: 0.694 ± 0.018	0.638TrpThr: 0.638 ± 0.019	0.933TrpVal: 0.933 ± 0.019	0.215TrpTrp: 0.215 ± 0.008	0.487TrpTyr: 0.487 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.622TyrAla: 2.622 ± 0.031	0.36TyrCys: 0.36 ± 0.011	2.153TyrAsp: 2.153 ± 0.026	2.3TyrGlu: 2.3 ± 0.027	2.361TyrPhe: 2.361 ± 0.033	2.275TyrGly: 2.275 ± 0.033	0.961TyrHis: 0.961 ± 0.018	2.479TyrIle: 2.479 ± 0.027	2.752TyrLys: 2.752 ± 0.036	4.392TyrLeu: 4.392 ± 0.04	0.701TyrMet: 0.701 ± 0.017	2.262TyrAsn: 2.262 ± 0.029	1.562TyrPro: 1.562 ± 0.025	2.505TyrGln: 2.505 ± 0.031	2.084TyrArg: 2.084 ± 0.024	2.315TyrSer: 2.315 ± 0.027	2.408TyrThr: 2.408 ± 0.034	2.107TyrVal: 2.107 ± 0.025	0.678TyrTrp: 0.678 ± 0.015	2.086TyrTyr: 2.086 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8907 proteins (3210568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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