Amino acid dipepetide frequency for Flavobacterium phage FL-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.347AlaAla: 4.347 ± 0.765
0.441AlaCys: 0.441 ± 0.194
3.024AlaAsp: 3.024 ± 0.468
4.095AlaGlu: 4.095 ± 0.781
2.457AlaPhe: 2.457 ± 0.446
4.788AlaGly: 4.788 ± 0.624
0.252AlaHis: 0.252 ± 0.119
5.229AlaIle: 5.229 ± 0.748
5.796AlaLys: 5.796 ± 0.759
5.355AlaLeu: 5.355 ± 0.703
1.764AlaMet: 1.764 ± 0.434
4.725AlaAsn: 4.725 ± 0.497
2.961AlaPro: 2.961 ± 2.042
2.331AlaGln: 2.331 ± 0.34
1.512AlaArg: 1.512 ± 0.301
4.41AlaSer: 4.41 ± 0.699
4.599AlaThr: 4.599 ± 0.851
3.528AlaVal: 3.528 ± 0.542
0.567AlaTrp: 0.567 ± 0.16
2.646AlaTyr: 2.646 ± 0.414
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.116
0.126CysCys: 0.126 ± 0.085
0.504CysAsp: 0.504 ± 0.172
0.882CysGlu: 0.882 ± 0.296
0.252CysPhe: 0.252 ± 0.12
0.441CysGly: 0.441 ± 0.161
0.189CysHis: 0.189 ± 0.1
0.63CysIle: 0.63 ± 0.215
0.756CysLys: 0.756 ± 0.256
0.567CysLeu: 0.567 ± 0.196
0.189CysMet: 0.189 ± 0.085
0.504CysAsn: 0.504 ± 0.17
0.315CysPro: 0.315 ± 0.178
0.315CysGln: 0.315 ± 0.16
0.189CysArg: 0.189 ± 0.111
0.504CysSer: 0.504 ± 0.145
0.378CysThr: 0.378 ± 0.151
0.378CysVal: 0.378 ± 0.151
0.063CysTrp: 0.063 ± 0.063
0.567CysTyr: 0.567 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
3.339AspAla: 3.339 ± 0.402
0.315AspCys: 0.315 ± 0.136
3.465AspAsp: 3.465 ± 0.592
3.78AspGlu: 3.78 ± 0.558
3.402AspPhe: 3.402 ± 0.43
3.591AspGly: 3.591 ± 0.514
0.567AspHis: 0.567 ± 0.169
4.599AspIle: 4.599 ± 0.495
4.536AspLys: 4.536 ± 0.652
5.985AspLeu: 5.985 ± 0.559
1.071AspMet: 1.071 ± 0.222
3.528AspAsn: 3.528 ± 0.472
1.386AspPro: 1.386 ± 0.295
2.016AspGln: 2.016 ± 0.469
2.331AspArg: 2.331 ± 0.421
3.717AspSer: 3.717 ± 0.454
1.953AspThr: 1.953 ± 0.323
3.213AspVal: 3.213 ± 0.443
0.882AspTrp: 0.882 ± 0.171
2.961AspTyr: 2.961 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
4.473GluAla: 4.473 ± 0.676
0.63GluCys: 0.63 ± 0.245
3.591GluAsp: 3.591 ± 0.513
5.544GluGlu: 5.544 ± 0.935
2.961GluPhe: 2.961 ± 0.525
2.394GluGly: 2.394 ± 0.45
1.197GluHis: 1.197 ± 0.263
5.859GluIle: 5.859 ± 0.791
7.308GluLys: 7.308 ± 1.013
6.615GluLeu: 6.615 ± 0.737
2.079GluMet: 2.079 ± 0.348
3.591GluAsn: 3.591 ± 0.51
0.945GluPro: 0.945 ± 0.244
2.079GluGln: 2.079 ± 0.362
2.268GluArg: 2.268 ± 0.406
5.67GluSer: 5.67 ± 0.779
3.843GluThr: 3.843 ± 0.517
4.347GluVal: 4.347 ± 0.462
0.504GluTrp: 0.504 ± 0.135
2.583GluTyr: 2.583 ± 0.426
0.0GluXaa: 0.0 ± 0.0
Phe
2.205PheAla: 2.205 ± 0.33
0.504PheCys: 0.504 ± 0.161
2.961PheAsp: 2.961 ± 0.444
3.78PheGlu: 3.78 ± 0.462
1.953PhePhe: 1.953 ± 0.394
2.646PheGly: 2.646 ± 0.443
0.693PheHis: 0.693 ± 0.185
3.591PheIle: 3.591 ± 0.478
4.032PheLys: 4.032 ± 0.448
3.276PheLeu: 3.276 ± 0.418
1.26PheMet: 1.26 ± 0.242
3.969PheAsn: 3.969 ± 0.491
1.89PhePro: 1.89 ± 0.317
1.701PheGln: 1.701 ± 0.28
1.764PheArg: 1.764 ± 0.276
3.276PheSer: 3.276 ± 0.468
2.898PheThr: 2.898 ± 0.378
2.52PheVal: 2.52 ± 0.353
0.567PheTrp: 0.567 ± 0.16
1.764PheTyr: 1.764 ± 0.386
0.0PheXaa: 0.0 ± 0.0
Gly
4.41GlyAla: 4.41 ± 0.683
0.441GlyCys: 0.441 ± 0.152
3.276GlyAsp: 3.276 ± 0.411
3.402GlyGlu: 3.402 ± 0.443
3.969GlyPhe: 3.969 ± 0.518
4.599GlyGly: 4.599 ± 0.547
0.693GlyHis: 0.693 ± 0.192
5.103GlyIle: 5.103 ± 0.697
4.725GlyLys: 4.725 ± 0.785
4.347GlyLeu: 4.347 ± 0.691
1.071GlyMet: 1.071 ± 0.25
3.717GlyAsn: 3.717 ± 0.471
0.819GlyPro: 0.819 ± 0.191
2.268GlyGln: 2.268 ± 0.431
2.646GlyArg: 2.646 ± 0.397
4.662GlySer: 4.662 ± 0.594
5.607GlyThr: 5.607 ± 0.798
4.347GlyVal: 4.347 ± 0.596
1.134GlyTrp: 1.134 ± 0.228
1.638GlyTyr: 1.638 ± 0.332
0.0GlyXaa: 0.0 ± 0.0
His
0.693HisAla: 0.693 ± 0.226
0.189HisCys: 0.189 ± 0.105
0.693HisAsp: 0.693 ± 0.224
0.567HisGlu: 0.567 ± 0.207
0.819HisPhe: 0.819 ± 0.229
1.134HisGly: 1.134 ± 0.345
0.252HisHis: 0.252 ± 0.127
1.071HisIle: 1.071 ± 0.253
1.071HisLys: 1.071 ± 0.266
1.386HisLeu: 1.386 ± 0.273
0.063HisMet: 0.063 ± 0.063
0.63HisAsn: 0.63 ± 0.201
0.378HisPro: 0.378 ± 0.138
0.378HisGln: 0.378 ± 0.158
0.63HisArg: 0.63 ± 0.201
0.819HisSer: 0.819 ± 0.212
0.882HisThr: 0.882 ± 0.268
0.693HisVal: 0.693 ± 0.314
0.315HisTrp: 0.315 ± 0.122
0.567HisTyr: 0.567 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.607IleAla: 5.607 ± 1.102
0.63IleCys: 0.63 ± 0.184
5.544IleAsp: 5.544 ± 0.55
5.67IleGlu: 5.67 ± 0.554
2.646IlePhe: 2.646 ± 0.457
4.473IleGly: 4.473 ± 0.528
1.197IleHis: 1.197 ± 0.287
5.418IleIle: 5.418 ± 0.577
6.993IleLys: 6.993 ± 0.587
5.922IleLeu: 5.922 ± 0.623
1.512IleMet: 1.512 ± 0.362
4.914IleAsn: 4.914 ± 0.625
2.961IlePro: 2.961 ± 0.376
2.835IleGln: 2.835 ± 0.391
3.654IleArg: 3.654 ± 0.465
6.237IleSer: 6.237 ± 0.624
4.977IleThr: 4.977 ± 0.568
4.662IleVal: 4.662 ± 0.726
0.504IleTrp: 0.504 ± 0.148
2.772IleTyr: 2.772 ± 0.426
0.0IleXaa: 0.0 ± 0.0
Lys
5.166LysAla: 5.166 ± 0.7
0.945LysCys: 0.945 ± 0.246
4.788LysAsp: 4.788 ± 0.507
5.985LysGlu: 5.985 ± 0.915
3.087LysPhe: 3.087 ± 0.429
4.725LysGly: 4.725 ± 0.489
1.323LysHis: 1.323 ± 0.299
6.615LysIle: 6.615 ± 0.731
7.876LysLys: 7.876 ± 0.983
6.048LysLeu: 6.048 ± 0.695
2.457LysMet: 2.457 ± 0.409
5.481LysAsn: 5.481 ± 0.558
2.835LysPro: 2.835 ± 0.464
2.709LysGln: 2.709 ± 0.402
3.087LysArg: 3.087 ± 0.433
5.796LysSer: 5.796 ± 0.638
5.733LysThr: 5.733 ± 0.578
5.166LysVal: 5.166 ± 0.6
1.323LysTrp: 1.323 ± 0.28
3.591LysTyr: 3.591 ± 0.515
0.0LysXaa: 0.0 ± 0.0
Leu
5.355LeuAla: 5.355 ± 0.633
0.882LeuCys: 0.882 ± 0.211
5.166LeuAsp: 5.166 ± 0.576
5.985LeuGlu: 5.985 ± 0.824
3.339LeuPhe: 3.339 ± 0.436
5.166LeuGly: 5.166 ± 0.508
0.882LeuHis: 0.882 ± 0.246
7.119LeuIle: 7.119 ± 0.725
7.686LeuLys: 7.686 ± 0.772
6.111LeuLeu: 6.111 ± 0.825
2.205LeuMet: 2.205 ± 0.375
5.481LeuAsn: 5.481 ± 0.611
2.142LeuPro: 2.142 ± 0.295
3.276LeuGln: 3.276 ± 0.396
2.835LeuArg: 2.835 ± 0.432
5.481LeuSer: 5.481 ± 0.621
4.977LeuThr: 4.977 ± 0.694
4.662LeuVal: 4.662 ± 0.551
1.071LeuTrp: 1.071 ± 0.242
2.709LeuTyr: 2.709 ± 0.399
0.0LeuXaa: 0.0 ± 0.0
Met
1.764MetAla: 1.764 ± 0.343
0.126MetCys: 0.126 ± 0.088
0.945MetAsp: 0.945 ± 0.268
1.26MetGlu: 1.26 ± 0.262
0.945MetPhe: 0.945 ± 0.254
1.512MetGly: 1.512 ± 0.305
0.315MetHis: 0.315 ± 0.115
1.953MetIle: 1.953 ± 0.329
2.772MetLys: 2.772 ± 0.477
1.134MetLeu: 1.134 ± 0.265
0.189MetMet: 0.189 ± 0.098
2.079MetAsn: 2.079 ± 0.334
0.945MetPro: 0.945 ± 0.214
0.693MetGln: 0.693 ± 0.163
0.945MetArg: 0.945 ± 0.251
2.079MetSer: 2.079 ± 0.249
1.575MetThr: 1.575 ± 0.293
1.197MetVal: 1.197 ± 0.22
0.378MetTrp: 0.378 ± 0.142
0.63MetTyr: 0.63 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
2.898AsnAla: 2.898 ± 0.377
0.441AsnCys: 0.441 ± 0.173
3.591AsnAsp: 3.591 ± 0.477
3.906AsnGlu: 3.906 ± 0.477
2.961AsnPhe: 2.961 ± 0.501
3.717AsnGly: 3.717 ± 0.581
0.756AsnHis: 0.756 ± 0.24
5.04AsnIle: 5.04 ± 0.673
4.914AsnLys: 4.914 ± 0.497
5.67AsnLeu: 5.67 ± 0.57
1.449AsnMet: 1.449 ± 0.259
4.347AsnAsn: 4.347 ± 0.582
1.512AsnPro: 1.512 ± 0.274
2.142AsnGln: 2.142 ± 0.352
2.583AsnArg: 2.583 ± 0.398
5.481AsnSer: 5.481 ± 0.678
4.851AsnThr: 4.851 ± 0.528
4.221AsnVal: 4.221 ± 0.486
0.441AsnTrp: 0.441 ± 0.167
2.268AsnTyr: 2.268 ± 0.39
0.0AsnXaa: 0.0 ± 0.0
Pro
1.827ProAla: 1.827 ± 0.338
0.126ProCys: 0.126 ± 0.091
1.701ProAsp: 1.701 ± 0.331
2.331ProGlu: 2.331 ± 0.36
1.764ProPhe: 1.764 ± 0.345
1.701ProGly: 1.701 ± 0.24
0.441ProHis: 0.441 ± 0.154
3.213ProIle: 3.213 ± 1.045
2.331ProLys: 2.331 ± 0.365
2.646ProLeu: 2.646 ± 0.357
0.378ProMet: 0.378 ± 0.157
1.512ProAsn: 1.512 ± 0.292
1.008ProPro: 1.008 ± 0.231
1.071ProGln: 1.071 ± 0.285
0.504ProArg: 0.504 ± 0.172
1.764ProSer: 1.764 ± 0.295
2.331ProThr: 2.331 ± 0.421
2.709ProVal: 2.709 ± 1.108
0.189ProTrp: 0.189 ± 0.11
0.945ProTyr: 0.945 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
2.331GlnAla: 2.331 ± 0.427
0.063GlnCys: 0.063 ± 0.061
1.953GlnAsp: 1.953 ± 0.341
2.016GlnGlu: 2.016 ± 0.377
1.638GlnPhe: 1.638 ± 0.354
1.89GlnGly: 1.89 ± 0.399
0.63GlnHis: 0.63 ± 0.275
3.213GlnIle: 3.213 ± 0.472
2.52GlnLys: 2.52 ± 0.361
3.591GlnLeu: 3.591 ± 0.565
1.323GlnMet: 1.323 ± 0.221
2.394GlnAsn: 2.394 ± 0.407
1.197GlnPro: 1.197 ± 0.26
0.882GlnGln: 0.882 ± 0.242
1.134GlnArg: 1.134 ± 0.341
2.583GlnSer: 2.583 ± 0.382
1.827GlnThr: 1.827 ± 0.308
2.079GlnVal: 2.079 ± 0.329
0.441GlnTrp: 0.441 ± 0.191
1.071GlnTyr: 1.071 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
2.079ArgAla: 2.079 ± 0.352
0.378ArgCys: 0.378 ± 0.163
1.512ArgAsp: 1.512 ± 0.34
2.583ArgGlu: 2.583 ± 0.391
2.709ArgPhe: 2.709 ± 0.379
2.079ArgGly: 2.079 ± 0.375
0.693ArgHis: 0.693 ± 0.21
2.394ArgIle: 2.394 ± 0.371
2.583ArgLys: 2.583 ± 0.356
3.528ArgLeu: 3.528 ± 0.579
0.693ArgMet: 0.693 ± 0.217
1.953ArgAsn: 1.953 ± 0.345
1.008ArgPro: 1.008 ± 0.238
1.512ArgGln: 1.512 ± 0.298
1.26ArgArg: 1.26 ± 0.267
2.646ArgSer: 2.646 ± 0.444
1.512ArgThr: 1.512 ± 0.362
2.646ArgVal: 2.646 ± 0.432
0.315ArgTrp: 0.315 ± 0.134
1.449ArgTyr: 1.449 ± 0.292
0.0ArgXaa: 0.0 ± 0.0
Ser
4.284SerAla: 4.284 ± 0.548
0.693SerCys: 0.693 ± 0.197
4.536SerAsp: 4.536 ± 0.652
5.04SerGlu: 5.04 ± 0.631
2.835SerPhe: 2.835 ± 0.327
6.867SerGly: 6.867 ± 0.768
0.882SerHis: 0.882 ± 0.321
4.851SerIle: 4.851 ± 0.583
5.481SerLys: 5.481 ± 0.713
6.426SerLeu: 6.426 ± 0.616
1.827SerMet: 1.827 ± 0.283
3.843SerAsn: 3.843 ± 0.487
1.827SerPro: 1.827 ± 0.304
2.457SerGln: 2.457 ± 0.329
2.142SerArg: 2.142 ± 0.402
4.788SerSer: 4.788 ± 0.532
4.599SerThr: 4.599 ± 0.551
5.607SerVal: 5.607 ± 0.631
0.63SerTrp: 0.63 ± 0.214
2.016SerTyr: 2.016 ± 0.281
0.0SerXaa: 0.0 ± 0.0
Thr
5.733ThrAla: 5.733 ± 0.859
0.315ThrCys: 0.315 ± 0.135
2.646ThrAsp: 2.646 ± 0.324
4.473ThrGlu: 4.473 ± 0.582
2.709ThrPhe: 2.709 ± 0.357
4.977ThrGly: 4.977 ± 0.603
0.504ThrHis: 0.504 ± 0.148
5.292ThrIle: 5.292 ± 0.571
5.292ThrLys: 5.292 ± 0.637
4.977ThrLeu: 4.977 ± 0.62
1.134ThrMet: 1.134 ± 0.252
3.78ThrAsn: 3.78 ± 0.629
2.457ThrPro: 2.457 ± 0.365
2.331ThrGln: 2.331 ± 0.469
1.512ThrArg: 1.512 ± 0.297
3.465ThrSer: 3.465 ± 0.433
3.906ThrThr: 3.906 ± 0.609
4.095ThrVal: 4.095 ± 0.653
0.378ThrTrp: 0.378 ± 0.176
2.772ThrTyr: 2.772 ± 0.464
0.0ThrXaa: 0.0 ± 0.0
Val
4.788ValAla: 4.788 ± 1.463
0.504ValCys: 0.504 ± 0.155
3.717ValAsp: 3.717 ± 0.447
4.095ValGlu: 4.095 ± 0.52
3.15ValPhe: 3.15 ± 0.409
3.528ValGly: 3.528 ± 0.53
1.071ValHis: 1.071 ± 0.228
4.221ValIle: 4.221 ± 0.496
4.788ValLys: 4.788 ± 0.681
4.851ValLeu: 4.851 ± 0.515
1.386ValMet: 1.386 ± 0.223
3.591ValAsn: 3.591 ± 0.433
2.268ValPro: 2.268 ± 0.339
1.89ValGln: 1.89 ± 0.336
2.457ValArg: 2.457 ± 0.352
4.788ValSer: 4.788 ± 0.552
3.717ValThr: 3.717 ± 0.6
3.78ValVal: 3.78 ± 0.44
0.693ValTrp: 0.693 ± 0.2
2.961ValTyr: 2.961 ± 0.517
0.0ValXaa: 0.0 ± 0.0
Trp
0.693TrpAla: 0.693 ± 0.215
0.063TrpCys: 0.063 ± 0.057
0.693TrpAsp: 0.693 ± 0.233
0.819TrpGlu: 0.819 ± 0.213
1.134TrpPhe: 1.134 ± 0.253
0.441TrpGly: 0.441 ± 0.174
0.126TrpHis: 0.126 ± 0.086
0.693TrpIle: 0.693 ± 0.193
0.945TrpLys: 0.945 ± 0.234
1.071TrpLeu: 1.071 ± 0.226
0.378TrpMet: 0.378 ± 0.163
0.819TrpAsn: 0.819 ± 0.217
0.189TrpPro: 0.189 ± 0.098
0.378TrpGln: 0.378 ± 0.159
0.378TrpArg: 0.378 ± 0.152
0.819TrpSer: 0.819 ± 0.232
0.63TrpThr: 0.63 ± 0.155
0.567TrpVal: 0.567 ± 0.195
0.126TrpTrp: 0.126 ± 0.099
0.252TrpTyr: 0.252 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.52TyrAla: 2.52 ± 0.321
0.252TyrCys: 0.252 ± 0.119
2.331TyrAsp: 2.331 ± 0.33
2.268TyrGlu: 2.268 ± 0.355
2.52TyrPhe: 2.52 ± 0.365
2.205TyrGly: 2.205 ± 0.385
0.567TyrHis: 0.567 ± 0.166
3.024TyrIle: 3.024 ± 0.453
2.331TyrLys: 2.331 ± 0.338
3.024TyrLeu: 3.024 ± 0.487
0.945TyrMet: 0.945 ± 0.263
2.331TyrAsn: 2.331 ± 0.428
1.449TyrPro: 1.449 ± 0.301
1.512TyrGln: 1.512 ± 0.301
1.701TyrArg: 1.701 ± 0.296
2.646TyrSer: 2.646 ± 0.397
1.953TyrThr: 1.953 ± 0.361
1.827TyrVal: 1.827 ± 0.319
0.693TyrTrp: 0.693 ± 0.187
0.819TyrTyr: 0.819 ± 0.217
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (15873 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski