Streptococcus satellite phage Javan728

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTZ5|A0A4D5ZTZ5_9VIRU DNA-damage-inducible protein D OS=Streptococcus satellite phage Javan728 OX=2558822 GN=JavanS728_0002 PE=4 SV=1
MM1 pKa = 7.44NNKK4 pKa = 8.74QVYY7 pKa = 8.26QAQTTDD13 pKa = 2.91HH14 pKa = 6.88GIARR18 pKa = 11.84ALGEE22 pKa = 4.87EE23 pKa = 3.69IRR25 pKa = 11.84MITDD29 pKa = 3.35VEE31 pKa = 4.09EE32 pKa = 4.08TTYY35 pKa = 11.03NGHH38 pKa = 6.1GEE40 pKa = 4.18IIVEE44 pKa = 4.21YY45 pKa = 9.21TSDD48 pKa = 3.37VALTKK53 pKa = 10.9LFDD56 pKa = 4.94RR57 pKa = 11.84IKK59 pKa = 10.69ALYY62 pKa = 9.24EE63 pKa = 3.93KK64 pKa = 10.91GEE66 pKa = 4.34SYY68 pKa = 9.02DD69 pKa = 3.61TKK71 pKa = 10.69PFLHH75 pKa = 6.56FKK77 pKa = 10.56VLEE80 pKa = 4.86DD81 pKa = 3.71DD82 pKa = 4.64TEE84 pKa = 4.28PSPNGVFTAKK94 pKa = 9.9EE95 pKa = 3.79VAEE98 pKa = 4.05FLGII102 pKa = 3.93

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZYW7|A0A4D5ZYW7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan728 OX=2558822 GN=JavanS728_0013 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.36RR3 pKa = 11.84IATIFLLGLPKK14 pKa = 9.96VPKK17 pKa = 8.88MRR19 pKa = 11.84PIAYY23 pKa = 8.55PRR25 pKa = 11.84ATISNNPEE33 pKa = 3.14ARR35 pKa = 11.84GIYY38 pKa = 10.11LEE40 pKa = 4.06NKK42 pKa = 9.02KK43 pKa = 10.62

Molecular weight: 4.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

3875

42

492

143.5

16.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.548 ± 0.208

0.852 ± 0.164

5.626 ± 0.485

8.8 ± 0.54

4.129 ± 0.289

5.29 ± 0.267

1.574 ± 0.247

6.761 ± 0.376

9.419 ± 0.558

10.245 ± 0.505

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.632 ± 0.277

5.342 ± 0.59

3.277 ± 0.376

3.69 ± 0.295

5.523 ± 0.416

5.032 ± 0.357

6.116 ± 0.385

4.826 ± 0.367

0.852 ± 0.143

4.465 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski