Cylindrospermum stagnale PCC 7417

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Cylindrospermum; Cylindrospermum stagnale

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9WYI7|K9WYI7_9NOST PEP-CTERM putative exosortase interaction domain-containing protein OS=Cylindrospermum stagnale PCC 7417 OX=56107 GN=Cylst_3099 PE=4 SV=1
MM1 pKa = 5.87MTKK4 pKa = 10.46YY5 pKa = 10.47SDD7 pKa = 3.77EE8 pKa = 4.79EE9 pKa = 3.81ILTFAKK15 pKa = 10.54SEE17 pKa = 3.79IKK19 pKa = 10.4FYY21 pKa = 9.96LTHH24 pKa = 6.9EE25 pKa = 4.5SAHH28 pKa = 6.42PNYY31 pKa = 10.39EE32 pKa = 3.77YY33 pKa = 11.1DD34 pKa = 5.05DD35 pKa = 4.81DD36 pKa = 6.27DD37 pKa = 5.94EE38 pKa = 6.79IISDD42 pKa = 5.07DD43 pKa = 3.41IGCTVFSSCTSFEE56 pKa = 4.37VKK58 pKa = 10.28DD59 pKa = 3.55AAIDD63 pKa = 3.8PEE65 pKa = 5.0HH66 pKa = 6.73ISGYY70 pKa = 7.01EE71 pKa = 3.64NCVAGVIVTVDD82 pKa = 3.42WSWSSEE88 pKa = 4.28DD89 pKa = 3.32EE90 pKa = 5.1DD91 pKa = 3.83EE92 pKa = 4.56PQFEE96 pKa = 4.59EE97 pKa = 5.11DD98 pKa = 3.64VEE100 pKa = 4.24LLIEE104 pKa = 4.72IYY106 pKa = 10.69DD107 pKa = 3.74VDD109 pKa = 5.44DD110 pKa = 4.07EE111 pKa = 4.77LSANRR116 pKa = 11.84RR117 pKa = 11.84EE118 pKa = 4.2IEE120 pKa = 4.11RR121 pKa = 11.84VV122 pKa = 3.18

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9X2A7|K9X2A7_9NOST Uncharacterized protein OS=Cylindrospermum stagnale PCC 7417 OX=56107 GN=Cylst_4103 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.21GRR28 pKa = 11.84NVISARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.16GRR39 pKa = 11.84HH40 pKa = 4.95RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6200

0

6200

2006691

27

4325

323.7

36.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.08 ± 0.031

0.987 ± 0.01

4.846 ± 0.022

6.293 ± 0.032

3.959 ± 0.021

6.482 ± 0.033

1.796 ± 0.017

6.823 ± 0.026

5.082 ± 0.029

11.071 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.013

4.544 ± 0.038

4.694 ± 0.029

5.491 ± 0.031

4.943 ± 0.026

6.494 ± 0.029

5.669 ± 0.033

6.503 ± 0.025

1.417 ± 0.016

3.066 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski