Niakha virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sripuvirus; Niakha sripuvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZRL9|R9ZRL9_9RHAB M OS=Niakha virus OX=1348439 PE=4 SV=1
MM1 pKa = 7.37SFDD4 pKa = 3.77AFEE7 pKa = 4.48FPSDD11 pKa = 3.27IAKK14 pKa = 10.27VAIGTAIKK22 pKa = 10.31ADD24 pKa = 3.14IMEE27 pKa = 4.5EE28 pKa = 4.12EE29 pKa = 4.28NDD31 pKa = 3.63VEE33 pKa = 4.54YY34 pKa = 8.53TTDD37 pKa = 3.27AAEE40 pKa = 4.62AKK42 pKa = 8.78TKK44 pKa = 10.47QDD46 pKa = 2.83IQIPEE51 pKa = 4.28SEE53 pKa = 5.03GGWTWEE59 pKa = 4.02NPVEE63 pKa = 4.4SGSKK67 pKa = 10.12GKK69 pKa = 9.79EE70 pKa = 3.99KK71 pKa = 9.77KK72 pKa = 8.72TLTYY76 pKa = 10.86SSDD79 pKa = 3.51EE80 pKa = 4.06EE81 pKa = 4.71SGNEE85 pKa = 3.89TDD87 pKa = 5.67DD88 pKa = 5.29KK89 pKa = 11.33EE90 pKa = 4.25DD91 pKa = 4.06SKK93 pKa = 11.75DD94 pKa = 3.27QTEE97 pKa = 4.12ITEE100 pKa = 4.36SGDD103 pKa = 3.27TFGPLEE109 pKa = 4.05YY110 pKa = 9.91PKK112 pKa = 9.76MVEE115 pKa = 3.44LTYY118 pKa = 10.07PSKK121 pKa = 10.89SFRR124 pKa = 11.84NKK126 pKa = 10.71DD127 pKa = 3.27PGKK130 pKa = 9.81LFEE133 pKa = 4.2WAITSLFDD141 pKa = 3.39QLGVRR146 pKa = 11.84KK147 pKa = 9.27LHH149 pKa = 6.59FKK151 pKa = 9.81TDD153 pKa = 3.62RR154 pKa = 11.84KK155 pKa = 10.7KK156 pKa = 10.1GTCQIMFDD164 pKa = 5.02GINSLKK170 pKa = 10.31FGRR173 pKa = 11.84ALEE176 pKa = 4.19PEE178 pKa = 3.95QLAFTKK184 pKa = 10.41KK185 pKa = 10.24PEE187 pKa = 4.22TPPPHH192 pKa = 7.07SSPKK196 pKa = 9.97KK197 pKa = 8.21PSAPRR202 pKa = 11.84LVEE205 pKa = 4.02VIEE208 pKa = 4.25AQKK211 pKa = 9.07EE212 pKa = 4.17TRR214 pKa = 11.84TVFKK218 pKa = 10.44EE219 pKa = 4.55GPSPQKK225 pKa = 9.95MQFLLEE231 pKa = 4.18LKK233 pKa = 10.38KK234 pKa = 10.74GIRR237 pKa = 11.84VRR239 pKa = 11.84DD240 pKa = 3.4IEE242 pKa = 4.8GDD244 pKa = 3.85MVCVRR249 pKa = 11.84LRR251 pKa = 11.84DD252 pKa = 3.56LAISEE257 pKa = 4.41EE258 pKa = 4.43EE259 pKa = 4.01VLGTEE264 pKa = 5.09FSGDD268 pKa = 3.57MDD270 pKa = 4.02PVTWLSEE277 pKa = 4.11LKK279 pKa = 10.79DD280 pKa = 3.55QFPHH284 pKa = 6.96LKK286 pKa = 10.19RR287 pKa = 11.84ILAMVDD293 pKa = 3.95FEE295 pKa = 4.62EE296 pKa = 4.55PP297 pKa = 3.04

Molecular weight: 33.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9ZRW4|R9ZRW4_9RHAB GDP polyribonucleotidyltransferase OS=Niakha virus OX=1348439 PE=4 SV=1
MM1 pKa = 7.51NFLFKK6 pKa = 10.36KK7 pKa = 9.93KK8 pKa = 10.09KK9 pKa = 9.95SSEE12 pKa = 4.31SLWGNSSKK20 pKa = 11.24ALVSAPIMKK29 pKa = 10.02NYY31 pKa = 9.91KK32 pKa = 9.23VEE34 pKa = 4.49CKK36 pKa = 10.04IEE38 pKa = 4.23LNVHH42 pKa = 5.88NKK44 pKa = 9.32VNGPDD49 pKa = 4.48DD50 pKa = 3.33IVDD53 pKa = 3.96IYY55 pKa = 11.35RR56 pKa = 11.84DD57 pKa = 3.69YY58 pKa = 11.33CSNYY62 pKa = 9.71CGVIQSRR69 pKa = 11.84PLHH72 pKa = 5.87CFIMALGICHH82 pKa = 6.64IQRR85 pKa = 11.84KK86 pKa = 8.73QGMSQGGDD94 pKa = 3.36YY95 pKa = 10.96LCEE98 pKa = 3.98YY99 pKa = 9.44KK100 pKa = 11.0GKK102 pKa = 9.74IQLQLGEE109 pKa = 4.25GADD112 pKa = 3.71PGVCRR117 pKa = 11.84SYY119 pKa = 10.61QIHH122 pKa = 7.21RR123 pKa = 11.84KK124 pKa = 9.44RR125 pKa = 11.84ISHH128 pKa = 6.99PAMLAYY134 pKa = 9.73ILGNTVFEE142 pKa = 4.86EE143 pKa = 4.29ISDD146 pKa = 3.76EE147 pKa = 4.32GVGSVEE153 pKa = 4.1TMTKK157 pKa = 9.67EE158 pKa = 3.92GRR160 pKa = 11.84IEE162 pKa = 4.15FKK164 pKa = 10.8KK165 pKa = 10.63LVAASPFILRR175 pKa = 11.84SGKK178 pKa = 10.2SRR180 pKa = 11.84TQICARR186 pKa = 11.84PP187 pKa = 3.39

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3547

187

2106

709.4

81.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.891 ± 0.633

2.453 ± 0.302

5.469 ± 0.361

6.879 ± 0.86

4.877 ± 0.149

5.892 ± 0.853

2.058 ± 0.344

7.387 ± 0.797

7.753 ± 0.568

9.191 ± 0.883

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.083

4.793 ± 0.821

4.257 ± 0.629

3.327 ± 0.314

4.624 ± 0.438

8.007 ± 0.372

5.695 ± 0.442

5.639 ± 0.259

1.663 ± 0.191

3.383 ± 0.374
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski