Natronospirillum operosum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Natronospirillaceae; Natronospirillum

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0WC45|A0A4Z0WC45_9GAMM DMT family transporter OS=Natronospirillum operosum OX=2759953 GN=E4656_15490 PE=4 SV=1
MM1 pKa = 7.4SPCTHH6 pKa = 6.74KK7 pKa = 10.75PLLSHH12 pKa = 7.04LLVLLAVTALAACNPSTEE30 pKa = 4.48SGGNSDD36 pKa = 4.72NGNGDD41 pKa = 3.3GGGGNGGNDD50 pKa = 3.28NNGDD54 pKa = 4.11LVEE57 pKa = 4.8LDD59 pKa = 3.69YY60 pKa = 11.69GVGFVLVMDD69 pKa = 5.09PAPNQIALRR78 pKa = 11.84GYY80 pKa = 8.46GTGQIPEE87 pKa = 4.28TTRR90 pKa = 11.84LAVQVLDD97 pKa = 3.85NDD99 pKa = 4.24LNPVAGHH106 pKa = 6.65DD107 pKa = 3.54VSFAITDD114 pKa = 3.64STGGPEE120 pKa = 4.12LTRR123 pKa = 11.84DD124 pKa = 3.49SATTGSDD131 pKa = 3.11GSASTILRR139 pKa = 11.84SGTVQGTTQVEE150 pKa = 4.34VSMDD154 pKa = 3.7LLDD157 pKa = 4.15SNGNVIEE164 pKa = 4.04QDD166 pKa = 3.34VHH168 pKa = 6.47VATTQPVSMHH178 pKa = 5.78TAAPVTDD185 pKa = 3.88GMSLSADD192 pKa = 3.44VFNPEE197 pKa = 3.96GYY199 pKa = 9.72DD200 pKa = 3.47YY201 pKa = 11.61NGIPVQVTVHH211 pKa = 6.48LGDD214 pKa = 3.21IHH216 pKa = 7.18RR217 pKa = 11.84NPVADD222 pKa = 3.71GTQVNFLASTGLVDD236 pKa = 5.37PVCTTDD242 pKa = 5.03NGACTIEE249 pKa = 4.2WRR251 pKa = 11.84SAHH254 pKa = 6.05PRR256 pKa = 11.84GDD258 pKa = 3.14GRR260 pKa = 11.84VDD262 pKa = 2.9ILARR266 pKa = 11.84TNGNDD271 pKa = 3.72AFVDD275 pKa = 3.7TNGNSRR281 pKa = 11.84FDD283 pKa = 3.54LGEE286 pKa = 3.97SYY288 pKa = 10.56EE289 pKa = 4.21PQGEE293 pKa = 4.16PFVDD297 pKa = 3.87TEE299 pKa = 4.43GTGQFDD305 pKa = 3.64PGDD308 pKa = 4.08FFWDD312 pKa = 3.99LSGTGDD318 pKa = 3.97YY319 pKa = 10.66TPADD323 pKa = 3.89PGAHH327 pKa = 5.68YY328 pKa = 10.36KK329 pKa = 10.99GIGCTEE335 pKa = 4.19DD336 pKa = 3.69ALEE339 pKa = 5.16HH340 pKa = 5.23GHH342 pKa = 6.3CDD344 pKa = 2.97EE345 pKa = 4.98RR346 pKa = 11.84AVVFNNIVLVASGSDD361 pKa = 3.29LRR363 pKa = 11.84LDD365 pKa = 3.99PEE367 pKa = 4.32PTDD370 pKa = 3.45VSVSDD375 pKa = 3.35TVIYY379 pKa = 10.74QMVDD383 pKa = 2.85QNGNVPPAGSDD394 pKa = 3.42LSINCTDD401 pKa = 3.88ASATALDD408 pKa = 3.94SEE410 pKa = 4.88VPEE413 pKa = 4.27RR414 pKa = 11.84PGALFGPWPVVINYY428 pKa = 9.96GDD430 pKa = 4.21DD431 pKa = 3.8PGSCTLEE438 pKa = 3.95VQSPNGLPQRR448 pKa = 11.84YY449 pKa = 6.73TISVNN454 pKa = 3.15

Molecular weight: 47.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0WBI7|A0A4Z0WBI7_9GAMM Beta-N-acetylhexosaminidase OS=Natronospirillum operosum OX=2759953 GN=E4656_06875 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27NGRR28 pKa = 11.84KK29 pKa = 8.98ILAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.72GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3948

0

3948

1311596

22

4272

332.2

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.46 ± 0.042

0.879 ± 0.013

5.926 ± 0.039

6.186 ± 0.042

3.553 ± 0.027

7.659 ± 0.041

2.423 ± 0.021

4.934 ± 0.033

2.456 ± 0.035

11.364 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.022

2.893 ± 0.028

4.852 ± 0.032

4.688 ± 0.028

6.985 ± 0.039

5.617 ± 0.026

5.34 ± 0.04

7.127 ± 0.03

1.584 ± 0.019

2.515 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski