Gordonia phage GTE8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0N6Q1|A0A0K0N6Q1_9CAUD Uncharacterized protein OS=Gordonia phage GTE8 OX=1647475 GN=GTE8_59 PE=4 SV=1
MM1 pKa = 7.75TYY3 pKa = 10.61LPDD6 pKa = 3.98ARR8 pKa = 11.84DD9 pKa = 3.74VIATRR14 pKa = 11.84TLDD17 pKa = 3.7PVDD20 pKa = 4.31GFTLTAYY27 pKa = 7.66EE28 pKa = 4.46VPDD31 pKa = 3.92TDD33 pKa = 3.73STPIGQEE40 pKa = 4.0LEE42 pKa = 4.18NGGCYY47 pKa = 9.67AAADD51 pKa = 3.44IAAWRR56 pKa = 11.84DD57 pKa = 3.66DD58 pKa = 2.83EE59 pKa = 4.21WQYY62 pKa = 11.64VGVVVVASRR71 pKa = 11.84AGVSLGSAAIWGMDD85 pKa = 3.03TGDD88 pKa = 3.57YY89 pKa = 9.89WDD91 pKa = 5.3AIARR95 pKa = 11.84AEE97 pKa = 3.87GRR99 pKa = 11.84TPYY102 pKa = 10.7GVLDD106 pKa = 3.84NVPNAVTTGQPRR118 pKa = 11.84EE119 pKa = 4.31DD120 pKa = 3.57GGIDD124 pKa = 3.5YY125 pKa = 7.48VTRR128 pKa = 11.84DD129 pKa = 4.3DD130 pKa = 3.93FAHH133 pKa = 7.2GYY135 pKa = 9.33GADD138 pKa = 4.51LISEE142 pKa = 4.47ALDD145 pKa = 3.52DD146 pKa = 4.73ARR148 pKa = 11.84ATLSSVVAGDD158 pKa = 3.51VAPP161 pKa = 4.41

Molecular weight: 17.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0N6H1|A0A0K0N6H1_9CAUD Uncharacterized protein OS=Gordonia phage GTE8 OX=1647475 GN=GTE8_72 PE=4 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84GGSLNTRR13 pKa = 11.84KK14 pKa = 9.98KK15 pKa = 10.82GKK17 pKa = 10.05AKK19 pKa = 10.66NGAKK23 pKa = 9.07WGHH26 pKa = 5.7GFVPKK31 pKa = 10.29NAAARR36 pKa = 11.84KK37 pKa = 9.03LKK39 pKa = 10.66KK40 pKa = 10.41KK41 pKa = 10.3LDD43 pKa = 3.9RR44 pKa = 11.84NGKK47 pKa = 7.93RR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 8.79RR51 pKa = 11.84TSQGYY56 pKa = 7.89PKK58 pKa = 10.48RR59 pKa = 3.9

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

21514

41

1795

228.9

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.975 ± 0.406

0.944 ± 0.156

6.968 ± 0.31

6.284 ± 0.301

3.142 ± 0.166

8.557 ± 0.401

1.822 ± 0.14

4.904 ± 0.192

4.221 ± 0.219

7.53 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.103

3.193 ± 0.151

5.889 ± 0.185

3.296 ± 0.119

7.563 ± 0.378

5.489 ± 0.195

6.224 ± 0.213

7.172 ± 0.208

1.929 ± 0.12

2.612 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski