Amino acid dipepetide frequency for Calothrix sp. PCC 6303

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.66AlaAla: 6.66 ± 0.092
0.816AlaCys: 0.816 ± 0.021
3.776AlaAsp: 3.776 ± 0.054
4.952AlaGlu: 4.952 ± 0.058
2.803AlaPhe: 2.803 ± 0.043
5.121AlaGly: 5.121 ± 0.073
1.062AlaHis: 1.062 ± 0.029
6.564AlaIle: 6.564 ± 0.068
4.462AlaLys: 4.462 ± 0.063
7.774AlaLeu: 7.774 ± 0.075
1.611AlaMet: 1.611 ± 0.032
3.711AlaAsn: 3.711 ± 0.059
2.511AlaPro: 2.511 ± 0.042
3.453AlaGln: 3.453 ± 0.049
3.132AlaArg: 3.132 ± 0.044
4.941AlaSer: 4.941 ± 0.06
4.689AlaThr: 4.689 ± 0.056
5.206AlaVal: 5.206 ± 0.059
0.971AlaTrp: 0.971 ± 0.026
2.18AlaTyr: 2.18 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.579CysAla: 0.579 ± 0.017
0.163CysCys: 0.163 ± 0.01
0.622CysAsp: 0.622 ± 0.022
0.578CysGlu: 0.578 ± 0.019
0.442CysPhe: 0.442 ± 0.016
0.795CysGly: 0.795 ± 0.021
0.273CysHis: 0.273 ± 0.012
0.574CysIle: 0.574 ± 0.021
0.398CysLys: 0.398 ± 0.014
1.158CysLeu: 1.158 ± 0.028
0.156CysMet: 0.156 ± 0.01
0.428CysAsn: 0.428 ± 0.017
0.492CysPro: 0.492 ± 0.019
0.575CysGln: 0.575 ± 0.022
0.534CysArg: 0.534 ± 0.019
0.635CysSer: 0.635 ± 0.019
0.479CysThr: 0.479 ± 0.016
0.579CysVal: 0.579 ± 0.018
0.134CysTrp: 0.134 ± 0.008
0.345CysTyr: 0.345 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.765AspAla: 3.765 ± 0.048
0.525AspCys: 0.525 ± 0.017
2.371AspAsp: 2.371 ± 0.045
3.077AspGlu: 3.077 ± 0.043
2.542AspPhe: 2.542 ± 0.038
3.517AspGly: 3.517 ± 0.062
0.617AspHis: 0.617 ± 0.021
3.882AspIle: 3.882 ± 0.05
2.6AspLys: 2.6 ± 0.044
5.5AspLeu: 5.5 ± 0.063
0.796AspMet: 0.796 ± 0.024
2.257AspAsn: 2.257 ± 0.037
1.946AspPro: 1.946 ± 0.036
1.508AspGln: 1.508 ± 0.027
2.732AspArg: 2.732 ± 0.042
3.171AspSer: 3.171 ± 0.046
2.601AspThr: 2.601 ± 0.047
3.093AspVal: 3.093 ± 0.038
0.837AspTrp: 0.837 ± 0.027
1.819AspTyr: 1.819 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
5.072GluAla: 5.072 ± 0.055
0.498GluCys: 0.498 ± 0.018
2.718GluAsp: 2.718 ± 0.042
4.028GluGlu: 4.028 ± 0.061
2.503GluPhe: 2.503 ± 0.034
3.06GluGly: 3.06 ± 0.048
0.967GluHis: 0.967 ± 0.025
5.438GluIle: 5.438 ± 0.075
4.015GluLys: 4.015 ± 0.048
6.949GluLeu: 6.949 ± 0.072
1.285GluMet: 1.285 ± 0.027
3.335GluAsn: 3.335 ± 0.05
2.18GluPro: 2.18 ± 0.039
3.217GluGln: 3.217 ± 0.046
3.113GluArg: 3.113 ± 0.054
3.683GluSer: 3.683 ± 0.044
3.498GluThr: 3.498 ± 0.047
4.291GluVal: 4.291 ± 0.059
0.77GluTrp: 0.77 ± 0.022
1.938GluTyr: 1.938 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.16PheAla: 3.16 ± 0.045
0.544PheCys: 0.544 ± 0.019
2.27PheAsp: 2.27 ± 0.041
2.131PheGlu: 2.131 ± 0.035
1.733PhePhe: 1.733 ± 0.034
2.968PheGly: 2.968 ± 0.042
0.702PheHis: 0.702 ± 0.025
2.594PheIle: 2.594 ± 0.04
1.714PheLys: 1.714 ± 0.028
4.077PheLeu: 4.077 ± 0.052
0.689PheMet: 0.689 ± 0.023
1.883PheAsn: 1.883 ± 0.029
1.868PhePro: 1.868 ± 0.034
1.897PheGln: 1.897 ± 0.032
1.825PheArg: 1.825 ± 0.032
3.142PheSer: 3.142 ± 0.049
2.365PheThr: 2.365 ± 0.04
2.436PheVal: 2.436 ± 0.04
0.673PheTrp: 0.673 ± 0.021
1.368PheTyr: 1.368 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
4.481GlyAla: 4.481 ± 0.07
0.777GlyCys: 0.777 ± 0.021
3.429GlyAsp: 3.429 ± 0.055
4.387GlyGlu: 4.387 ± 0.056
2.986GlyPhe: 2.986 ± 0.042
4.813GlyGly: 4.813 ± 0.085
1.12GlyHis: 1.12 ± 0.03
5.231GlyIle: 5.231 ± 0.056
4.816GlyLys: 4.816 ± 0.068
6.567GlyLeu: 6.567 ± 0.068
1.502GlyMet: 1.502 ± 0.033
3.614GlyAsn: 3.614 ± 0.07
0.87GlyPro: 0.87 ± 0.024
2.51GlyGln: 2.51 ± 0.047
2.913GlyArg: 2.913 ± 0.043
4.135GlySer: 4.135 ± 0.064
3.757GlyThr: 3.757 ± 0.063
4.574GlyVal: 4.574 ± 0.049
1.05GlyTrp: 1.05 ± 0.025
2.37GlyTyr: 2.37 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
0.98HisAla: 0.98 ± 0.022
0.238HisCys: 0.238 ± 0.012
0.767HisAsp: 0.767 ± 0.023
0.95HisGlu: 0.95 ± 0.025
0.733HisPhe: 0.733 ± 0.023
1.088HisGly: 1.088 ± 0.027
0.595HisHis: 0.595 ± 0.024
1.079HisIle: 1.079 ± 0.023
0.776HisLys: 0.776 ± 0.019
2.113HisLeu: 2.113 ± 0.037
0.229HisMet: 0.229 ± 0.011
0.774HisAsn: 0.774 ± 0.023
1.243HisPro: 1.243 ± 0.031
1.143HisGln: 1.143 ± 0.027
0.967HisArg: 0.967 ± 0.02
1.13HisSer: 1.13 ± 0.029
0.914HisThr: 0.914 ± 0.025
0.83HisVal: 0.83 ± 0.023
0.285HisTrp: 0.285 ± 0.012
0.623HisTyr: 0.623 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.873IleAla: 6.873 ± 0.068
0.823IleCys: 0.823 ± 0.023
3.901IleAsp: 3.901 ± 0.048
4.366IleGlu: 4.366 ± 0.055
2.86IlePhe: 2.86 ± 0.045
4.55IleGly: 4.55 ± 0.057
1.355IleHis: 1.355 ± 0.03
4.546IleIle: 4.546 ± 0.058
3.74IleLys: 3.74 ± 0.053
7.187IleLeu: 7.187 ± 0.08
0.949IleMet: 0.949 ± 0.023
3.68IleAsn: 3.68 ± 0.061
3.903IlePro: 3.903 ± 0.045
3.459IleGln: 3.459 ± 0.052
3.246IleArg: 3.246 ± 0.046
5.249IleSer: 5.249 ± 0.061
4.146IleThr: 4.146 ± 0.062
4.21IleVal: 4.21 ± 0.047
0.958IleTrp: 0.958 ± 0.03
2.19IleTyr: 2.19 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
4.066LysAla: 4.066 ± 0.058
0.369LysCys: 0.369 ± 0.015
2.404LysAsp: 2.404 ± 0.04
3.009LysGlu: 3.009 ± 0.047
2.041LysPhe: 2.041 ± 0.036
2.823LysGly: 2.823 ± 0.042
0.903LysHis: 0.903 ± 0.025
4.357LysIle: 4.357 ± 0.052
2.811LysLys: 2.811 ± 0.051
6.189LysLeu: 6.189 ± 0.07
1.109LysMet: 1.109 ± 0.024
2.772LysAsn: 2.772 ± 0.045
2.646LysPro: 2.646 ± 0.046
3.053LysGln: 3.053 ± 0.046
2.4LysArg: 2.4 ± 0.038
3.638LysSer: 3.638 ± 0.052
3.273LysThr: 3.273 ± 0.044
3.372LysVal: 3.372 ± 0.039
0.568LysTrp: 0.568 ± 0.022
1.82LysTyr: 1.82 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
8.795LeuAla: 8.795 ± 0.089
1.007LeuCys: 1.007 ± 0.023
5.204LeuAsp: 5.204 ± 0.054
7.253LeuGlu: 7.253 ± 0.077
3.853LeuPhe: 3.853 ± 0.053
7.274LeuGly: 7.274 ± 0.077
1.736LeuHis: 1.736 ± 0.032
7.042LeuIle: 7.042 ± 0.074
5.832LeuLys: 5.832 ± 0.067
10.721LeuLeu: 10.721 ± 0.092
2.046LeuMet: 2.046 ± 0.036
4.944LeuAsn: 4.944 ± 0.05
5.399LeuPro: 5.399 ± 0.065
5.608LeuGln: 5.608 ± 0.071
5.288LeuArg: 5.288 ± 0.062
7.316LeuSer: 7.316 ± 0.073
6.223LeuThr: 6.223 ± 0.063
6.957LeuVal: 6.957 ± 0.067
1.369LeuTrp: 1.369 ± 0.04
2.646LeuTyr: 2.646 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.543MetAla: 1.543 ± 0.032
0.129MetCys: 0.129 ± 0.009
0.758MetAsp: 0.758 ± 0.021
1.099MetGlu: 1.099 ± 0.028
0.585MetPhe: 0.585 ± 0.019
1.418MetGly: 1.418 ± 0.032
0.292MetHis: 0.292 ± 0.013
1.085MetIle: 1.085 ± 0.026
1.032MetLys: 1.032 ± 0.025
1.947MetLeu: 1.947 ± 0.035
0.491MetMet: 0.491 ± 0.017
0.927MetAsn: 0.927 ± 0.024
0.86MetPro: 0.86 ± 0.023
0.986MetGln: 0.986 ± 0.024
1.05MetArg: 1.05 ± 0.024
1.378MetSer: 1.378 ± 0.025
1.236MetThr: 1.236 ± 0.031
1.313MetVal: 1.313 ± 0.03
0.174MetTrp: 0.174 ± 0.01
0.391MetTyr: 0.391 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.214AsnAla: 3.214 ± 0.052
0.506AsnCys: 0.506 ± 0.019
1.889AsnAsp: 1.889 ± 0.038
1.984AsnGlu: 1.984 ± 0.04
2.227AsnPhe: 2.227 ± 0.045
2.843AsnGly: 2.843 ± 0.059
0.989AsnHis: 0.989 ± 0.024
3.605AsnIle: 3.605 ± 0.061
2.186AsnLys: 2.186 ± 0.039
6.105AsnLeu: 6.105 ± 0.072
0.779AsnMet: 0.779 ± 0.024
2.672AsnAsn: 2.672 ± 0.049
3.211AsnPro: 3.211 ± 0.047
3.277AsnGln: 3.277 ± 0.057
2.48AsnArg: 2.48 ± 0.039
3.761AsnSer: 3.761 ± 0.053
2.578AsnThr: 2.578 ± 0.043
2.339AsnVal: 2.339 ± 0.036
0.857AsnTrp: 0.857 ± 0.021
1.727AsnTyr: 1.727 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
2.811ProAla: 2.811 ± 0.04
0.333ProCys: 0.333 ± 0.012
2.76ProAsp: 2.76 ± 0.042
3.646ProGlu: 3.646 ± 0.047
1.654ProPhe: 1.654 ± 0.03
2.948ProGly: 2.948 ± 0.04
0.831ProHis: 0.831 ± 0.023
3.116ProIle: 3.116 ± 0.041
2.334ProLys: 2.334 ± 0.038
4.256ProLeu: 4.256 ± 0.049
0.728ProMet: 0.728 ± 0.018
2.367ProAsn: 2.367 ± 0.039
1.977ProPro: 1.977 ± 0.048
2.568ProGln: 2.568 ± 0.046
1.594ProArg: 1.594 ± 0.029
2.951ProSer: 2.951 ± 0.044
2.677ProThr: 2.677 ± 0.044
3.022ProVal: 3.022 ± 0.05
0.543ProTrp: 0.543 ± 0.019
1.182ProTyr: 1.182 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.391GlnAla: 4.391 ± 0.057
0.329GlnCys: 0.329 ± 0.013
2.138GlnAsp: 2.138 ± 0.04
3.544GlnGlu: 3.544 ± 0.057
1.678GlnPhe: 1.678 ± 0.031
3.165GlnGly: 3.165 ± 0.042
0.873GlnHis: 0.873 ± 0.024
4.033GlnIle: 4.033 ± 0.05
3.068GlnLys: 3.068 ± 0.048
5.675GlnLeu: 5.675 ± 0.06
1.071GlnMet: 1.071 ± 0.025
2.478GlnAsn: 2.478 ± 0.034
2.455GlnPro: 2.455 ± 0.044
3.896GlnGln: 3.896 ± 0.058
2.815GlnArg: 2.815 ± 0.046
3.016GlnSer: 3.016 ± 0.045
2.873GlnThr: 2.873 ± 0.041
3.682GlnVal: 3.682 ± 0.047
0.607GlnTrp: 0.607 ± 0.019
1.263GlnTyr: 1.263 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
2.816ArgAla: 2.816 ± 0.047
0.519ArgCys: 0.519 ± 0.017
2.559ArgAsp: 2.559 ± 0.038
3.249ArgGlu: 3.249 ± 0.044
2.117ArgPhe: 2.117 ± 0.034
2.884ArgGly: 2.884 ± 0.047
0.914ArgHis: 0.914 ± 0.026
3.411ArgIle: 3.411 ± 0.044
2.421ArgLys: 2.421 ± 0.04
5.363ArgLeu: 5.363 ± 0.064
1.008ArgMet: 1.008 ± 0.023
2.282ArgAsn: 2.282 ± 0.042
1.715ArgPro: 1.715 ± 0.032
2.959ArgGln: 2.959 ± 0.044
2.705ArgArg: 2.705 ± 0.049
3.14ArgSer: 3.14 ± 0.045
2.387ArgThr: 2.387 ± 0.04
3.133ArgVal: 3.133 ± 0.041
0.717ArgTrp: 0.717 ± 0.019
1.8ArgTyr: 1.8 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
4.507SerAla: 4.507 ± 0.052
0.638SerCys: 0.638 ± 0.018
3.441SerAsp: 3.441 ± 0.044
3.932SerGlu: 3.932 ± 0.055
2.724SerPhe: 2.724 ± 0.046
4.931SerGly: 4.931 ± 0.068
1.357SerHis: 1.357 ± 0.03
4.327SerIle: 4.327 ± 0.052
3.142SerLys: 3.142 ± 0.041
7.539SerLeu: 7.539 ± 0.081
1.189SerMet: 1.189 ± 0.03
3.224SerAsn: 3.224 ± 0.05
3.383SerPro: 3.383 ± 0.056
4.175SerGln: 4.175 ± 0.059
3.3SerArg: 3.3 ± 0.047
4.892SerSer: 4.892 ± 0.062
3.792SerThr: 3.792 ± 0.045
4.1SerVal: 4.1 ± 0.05
0.934SerTrp: 0.934 ± 0.027
1.943SerTyr: 1.943 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
4.515ThrAla: 4.515 ± 0.058
0.497ThrCys: 0.497 ± 0.018
2.679ThrAsp: 2.679 ± 0.042
3.277ThrGlu: 3.277 ± 0.046
2.096ThrPhe: 2.096 ± 0.033
4.371ThrGly: 4.371 ± 0.067
0.971ThrHis: 0.971 ± 0.024
3.961ThrIle: 3.961 ± 0.052
2.703ThrLys: 2.703 ± 0.041
6.043ThrLeu: 6.043 ± 0.073
0.818ThrMet: 0.818 ± 0.022
2.724ThrAsn: 2.724 ± 0.05
3.369ThrPro: 3.369 ± 0.057
2.918ThrGln: 2.918 ± 0.044
2.287ThrArg: 2.287 ± 0.04
4.013ThrSer: 4.013 ± 0.055
3.599ThrThr: 3.599 ± 0.056
3.885ThrVal: 3.885 ± 0.049
0.703ThrTrp: 0.703 ± 0.019
1.618ThrTyr: 1.618 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
5.39ValAla: 5.39 ± 0.061
0.663ValCys: 0.663 ± 0.022
3.337ValAsp: 3.337 ± 0.048
4.4ValGlu: 4.4 ± 0.061
2.523ValPhe: 2.523 ± 0.04
4.411ValGly: 4.411 ± 0.056
0.902ValHis: 0.902 ± 0.024
4.637ValIle: 4.637 ± 0.058
3.545ValLys: 3.545 ± 0.05
6.024ValLeu: 6.024 ± 0.062
1.459ValMet: 1.459 ± 0.028
3.165ValAsn: 3.165 ± 0.046
2.64ValPro: 2.64 ± 0.036
2.571ValGln: 2.571 ± 0.038
3.049ValArg: 3.049 ± 0.045
4.373ValSer: 4.373 ± 0.052
3.86ValThr: 3.86 ± 0.05
4.649ValVal: 4.649 ± 0.063
0.769ValTrp: 0.769 ± 0.026
1.776ValTyr: 1.776 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.786TrpAla: 0.786 ± 0.022
0.163TrpCys: 0.163 ± 0.01
0.679TrpAsp: 0.679 ± 0.027
0.978TrpGlu: 0.978 ± 0.025
0.575TrpPhe: 0.575 ± 0.02
0.925TrpGly: 0.925 ± 0.025
0.331TrpHis: 0.331 ± 0.016
0.807TrpIle: 0.807 ± 0.019
0.655TrpLys: 0.655 ± 0.017
1.746TrpLeu: 1.746 ± 0.038
0.33TrpMet: 0.33 ± 0.015
0.675TrpAsn: 0.675 ± 0.024
0.082TrpPro: 0.082 ± 0.007
1.173TrpGln: 1.173 ± 0.026
0.803TrpArg: 0.803 ± 0.023
0.797TrpSer: 0.797 ± 0.024
0.551TrpThr: 0.551 ± 0.019
0.93TrpVal: 0.93 ± 0.023
0.228TrpTrp: 0.228 ± 0.013
0.438TrpTyr: 0.438 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.989TyrAla: 1.989 ± 0.037
0.4TyrCys: 0.4 ± 0.016
1.455TyrAsp: 1.455 ± 0.028
1.719TyrGlu: 1.719 ± 0.04
1.364TyrPhe: 1.364 ± 0.03
1.995TyrGly: 1.995 ± 0.037
0.668TyrHis: 0.668 ± 0.023
1.903TyrIle: 1.903 ± 0.036
1.373TyrLys: 1.373 ± 0.03
3.579TyrLeu: 3.579 ± 0.055
0.451TyrMet: 0.451 ± 0.019
1.316TyrAsn: 1.316 ± 0.029
1.546TyrPro: 1.546 ± 0.035
2.104TyrGln: 2.104 ± 0.037
1.868TyrArg: 1.868 ± 0.036
2.107TyrSer: 2.107 ± 0.035
1.55TyrThr: 1.55 ± 0.029
1.541TyrVal: 1.541 ± 0.034
0.509TyrTrp: 0.509 ± 0.015
1.109TyrTyr: 1.109 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5484 proteins (1811230 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski