Amino acid dipepetide frequency for Thermococcus barophilus (strain DSM 11836 / MP)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.997AlaAla: 3.997 ± 0.096	0.391AlaCys: 0.391 ± 0.027	2.731AlaAsp: 2.731 ± 0.077	5.27AlaGlu: 5.27 ± 0.085	3.648AlaPhe: 3.648 ± 0.075	4.603AlaGly: 4.603 ± 0.095	1.17AlaHis: 1.17 ± 0.042	5.748AlaIle: 5.748 ± 0.102	6.221AlaLys: 6.221 ± 0.118	8.761AlaLeu: 8.761 ± 0.13	1.884AlaMet: 1.884 ± 0.055	2.003AlaAsn: 2.003 ± 0.053	2.131AlaPro: 2.131 ± 0.063	1.64AlaGln: 1.64 ± 0.053	3.194AlaArg: 3.194 ± 0.073	2.969AlaSer: 2.969 ± 0.074	2.89AlaThr: 2.89 ± 0.071	5.78AlaVal: 5.78 ± 0.09	0.776AlaTrp: 0.776 ± 0.043	2.956AlaTyr: 2.956 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.269CysAla: 0.269 ± 0.022	0.057CysCys: 0.057 ± 0.01	0.282CysAsp: 0.282 ± 0.022	0.385CysGlu: 0.385 ± 0.027	0.198CysPhe: 0.198 ± 0.016	0.741CysGly: 0.741 ± 0.039	0.138CysHis: 0.138 ± 0.014	0.431CysIle: 0.431 ± 0.027	0.383CysLys: 0.383 ± 0.028	0.428CysLeu: 0.428 ± 0.026	0.131CysMet: 0.131 ± 0.014	0.223CysAsn: 0.223 ± 0.019	0.61CysPro: 0.61 ± 0.039	0.122CysGln: 0.122 ± 0.014	0.291CysArg: 0.291 ± 0.022	0.309CysSer: 0.309 ± 0.027	0.241CysThr: 0.241 ± 0.019	0.359CysVal: 0.359 ± 0.027	0.068CysTrp: 0.068 ± 0.008	0.188CysTyr: 0.188 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.031AspAla: 3.031 ± 0.076	0.238AspCys: 0.238 ± 0.022	1.948AspAsp: 1.948 ± 0.065	4.537AspGlu: 4.537 ± 0.09	2.326AspPhe: 2.326 ± 0.06	3.05AspGly: 3.05 ± 0.089	0.595AspHis: 0.595 ± 0.031	4.012AspIle: 4.012 ± 0.09	3.149AspLys: 3.149 ± 0.068	4.624AspLeu: 4.624 ± 0.083	1.07AspMet: 1.07 ± 0.039	1.376AspAsn: 1.376 ± 0.051	2.111AspPro: 2.111 ± 0.06	0.567AspGln: 0.567 ± 0.03	2.017AspArg: 2.017 ± 0.057	1.811AspSer: 1.811 ± 0.058	1.772AspThr: 1.772 ± 0.056	4.242AspVal: 4.242 ± 0.082	0.549AspTrp: 0.549 ± 0.032	2.207AspTyr: 2.207 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	5.156GluAla: 5.156 ± 0.102	0.382GluCys: 0.382 ± 0.026	3.947GluAsp: 3.947 ± 0.082	9.018GluGlu: 9.018 ± 0.187	4.0GluPhe: 4.0 ± 0.092	5.297GluGly: 5.297 ± 0.107	1.4GluHis: 1.4 ± 0.049	8.305GluIle: 8.305 ± 0.135	9.019GluLys: 9.019 ± 0.17	9.119GluLeu: 9.119 ± 0.17	2.082GluMet: 2.082 ± 0.063	3.723GluAsn: 3.723 ± 0.091	2.367GluPro: 2.367 ± 0.07	1.496GluGln: 1.496 ± 0.055	4.708GluArg: 4.708 ± 0.103	3.134GluSer: 3.134 ± 0.078	2.972GluThr: 2.972 ± 0.076	6.532GluVal: 6.532 ± 0.111	1.061GluTrp: 1.061 ± 0.039	3.192GluTyr: 3.192 ± 0.068	0.0GluXaa: 0.0 ± 0.0
Phe	3.262PheAla: 3.262 ± 0.077	0.283PheCys: 0.283 ± 0.022	2.305PheAsp: 2.305 ± 0.054	3.982PheGlu: 3.982 ± 0.078	2.003PhePhe: 2.003 ± 0.069	3.986PheGly: 3.986 ± 0.075	0.722PheHis: 0.722 ± 0.032	3.515PheIle: 3.515 ± 0.085	3.186PheLys: 3.186 ± 0.071	4.923PheLeu: 4.923 ± 0.112	1.137PheMet: 1.137 ± 0.044	1.595PheAsn: 1.595 ± 0.045	1.811PhePro: 1.811 ± 0.044	0.865PheGln: 0.865 ± 0.029	2.062PheArg: 2.062 ± 0.055	2.668PheSer: 2.668 ± 0.071	2.066PheThr: 2.066 ± 0.061	3.263PheVal: 3.263 ± 0.077	0.559PheTrp: 0.559 ± 0.03	1.975PheTyr: 1.975 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.565GlyAla: 4.565 ± 0.094	0.523GlyCys: 0.523 ± 0.035	3.252GlyAsp: 3.252 ± 0.069	5.461GlyGlu: 5.461 ± 0.107	3.585GlyPhe: 3.585 ± 0.088	4.492GlyGly: 4.492 ± 0.109	1.134GlyHis: 1.134 ± 0.039	7.418GlyIle: 7.418 ± 0.112	6.525GlyLys: 6.525 ± 0.105	5.96GlyLeu: 5.96 ± 0.1	1.883GlyMet: 1.883 ± 0.057	2.564GlyAsn: 2.564 ± 0.073	1.642GlyPro: 1.642 ± 0.054	1.159GlyGln: 1.159 ± 0.046	3.355GlyArg: 3.355 ± 0.078	3.313GlySer: 3.313 ± 0.079	3.298GlyThr: 3.298 ± 0.075	5.469GlyVal: 5.469 ± 0.099	0.941GlyTrp: 0.941 ± 0.04	3.132GlyTyr: 3.132 ± 0.085	0.0GlyXaa: 0.0 ± 0.0
His	1.096HisAla: 1.096 ± 0.046	0.13HisCys: 0.13 ± 0.014	0.698HisAsp: 0.698 ± 0.032	1.176HisGlu: 1.176 ± 0.043	0.803HisPhe: 0.803 ± 0.037	1.294HisGly: 1.294 ± 0.052	0.345HisHis: 0.345 ± 0.027	1.273HisIle: 1.273 ± 0.051	0.956HisLys: 0.956 ± 0.039	1.701HisLeu: 1.701 ± 0.049	0.382HisMet: 0.382 ± 0.026	0.472HisAsn: 0.472 ± 0.029	1.009HisPro: 1.009 ± 0.039	0.283HisGln: 0.283 ± 0.021	0.838HisArg: 0.838 ± 0.04	0.85HisSer: 0.85 ± 0.041	0.684HisThr: 0.684 ± 0.036	1.124HisVal: 1.124 ± 0.039	0.217HisTrp: 0.217 ± 0.02	0.796HisTyr: 0.796 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	6.833IleAla: 6.833 ± 0.121	0.47IleCys: 0.47 ± 0.03	4.028IleAsp: 4.028 ± 0.067	7.314IleGlu: 7.314 ± 0.139	3.65IlePhe: 3.65 ± 0.091	6.02IleGly: 6.02 ± 0.105	1.306IleHis: 1.306 ± 0.044	6.831IleIle: 6.831 ± 0.121	6.891IleLys: 6.891 ± 0.118	8.741IleLeu: 8.741 ± 0.147	1.838IleMet: 1.838 ± 0.052	2.904IleAsn: 2.904 ± 0.074	4.03IlePro: 4.03 ± 0.078	1.644IleGln: 1.644 ± 0.05	4.22IleArg: 4.22 ± 0.085	4.635IleSer: 4.635 ± 0.095	4.111IleThr: 4.111 ± 0.083	6.452IleVal: 6.452 ± 0.103	0.804IleTrp: 0.804 ± 0.032	3.164IleTyr: 3.164 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	6.579LysAla: 6.579 ± 0.111	0.526LysCys: 0.526 ± 0.035	3.86LysAsp: 3.86 ± 0.083	8.668LysGlu: 8.668 ± 0.167	3.339LysPhe: 3.339 ± 0.082	5.183LysGly: 5.183 ± 0.099	1.24LysHis: 1.24 ± 0.043	7.232LysIle: 7.232 ± 0.112	7.068LysLys: 7.068 ± 0.137	8.077LysLeu: 8.077 ± 0.13	1.71LysMet: 1.71 ± 0.056	3.067LysAsn: 3.067 ± 0.069	3.495LysPro: 3.495 ± 0.079	1.477LysGln: 1.477 ± 0.047	4.76LysArg: 4.76 ± 0.088	3.453LysSer: 3.453 ± 0.073	3.409LysThr: 3.409 ± 0.076	6.643LysVal: 6.643 ± 0.103	0.952LysTrp: 0.952 ± 0.046	2.939LysTyr: 2.939 ± 0.08	0.0LysXaa: 0.0 ± 0.0
Leu	7.838LeuAla: 7.838 ± 0.127	0.478LeuCys: 0.478 ± 0.029	4.595LeuAsp: 4.595 ± 0.089	8.413LeuGlu: 8.413 ± 0.147	4.313LeuPhe: 4.313 ± 0.102	7.691LeuGly: 7.691 ± 0.138	1.495LeuHis: 1.495 ± 0.047	8.506LeuIle: 8.506 ± 0.146	9.281LeuLys: 9.281 ± 0.14	10.194LeuLeu: 10.194 ± 0.178	2.668LeuMet: 2.668 ± 0.074	3.708LeuAsn: 3.708 ± 0.081	4.21LeuPro: 4.21 ± 0.083	2.046LeuGln: 2.046 ± 0.047	5.626LeuArg: 5.626 ± 0.107	6.025LeuSer: 6.025 ± 0.11	4.665LeuThr: 4.665 ± 0.079	6.43LeuVal: 6.43 ± 0.1	1.085LeuTrp: 1.085 ± 0.039	3.745LeuTyr: 3.745 ± 0.077	0.0LeuXaa: 0.0 ± 0.0
Met	1.688MetAla: 1.688 ± 0.046	0.117MetCys: 0.117 ± 0.014	0.969MetAsp: 0.969 ± 0.038	1.873MetGlu: 1.873 ± 0.053	0.917MetPhe: 0.917 ± 0.038	1.569MetGly: 1.569 ± 0.05	0.477MetHis: 0.477 ± 0.029	1.957MetIle: 1.957 ± 0.055	2.374MetLys: 2.374 ± 0.063	2.442MetLeu: 2.442 ± 0.067	0.545MetMet: 0.545 ± 0.031	0.844MetAsn: 0.844 ± 0.035	1.123MetPro: 1.123 ± 0.036	0.488MetGln: 0.488 ± 0.028	1.393MetArg: 1.393 ± 0.043	1.241MetSer: 1.241 ± 0.041	1.004MetThr: 1.004 ± 0.038	1.553MetVal: 1.553 ± 0.054	0.214MetTrp: 0.214 ± 0.018	0.652MetTyr: 0.652 ± 0.032	0.0MetXaa: 0.0 ± 0.0
Asn	2.605AsnAla: 2.605 ± 0.064	0.233AsnCys: 0.233 ± 0.021	1.305AsnAsp: 1.305 ± 0.046	2.822AsnGlu: 2.822 ± 0.058	1.637AsnPhe: 1.637 ± 0.049	2.668AsnGly: 2.668 ± 0.066	0.526AsnHis: 0.526 ± 0.029	3.064AsnIle: 3.064 ± 0.083	2.142AsnLys: 2.142 ± 0.061	3.887AsnLeu: 3.887 ± 0.082	0.627AsnMet: 0.627 ± 0.031	1.032AsnAsn: 1.032 ± 0.051	2.298AsnPro: 2.298 ± 0.063	0.665AsnGln: 0.665 ± 0.037	1.463AsnArg: 1.463 ± 0.053	1.62AsnSer: 1.62 ± 0.057	1.583AsnThr: 1.583 ± 0.061	3.024AsnVal: 3.024 ± 0.094	0.451AsnTrp: 0.451 ± 0.026	1.469AsnTyr: 1.469 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.172ProAla: 2.172 ± 0.064	0.244ProCys: 0.244 ± 0.02	1.956ProAsp: 1.956 ± 0.056	3.921ProGlu: 3.921 ± 0.088	1.921ProPhe: 1.921 ± 0.051	2.473ProGly: 2.473 ± 0.068	0.839ProHis: 0.839 ± 0.038	3.232ProIle: 3.232 ± 0.075	3.547ProLys: 3.547 ± 0.07	4.117ProLeu: 4.117 ± 0.079	0.874ProMet: 0.874 ± 0.041	1.547ProAsn: 1.547 ± 0.051	1.715ProPro: 1.715 ± 0.057	0.998ProGln: 0.998 ± 0.045	1.853ProArg: 1.853 ± 0.062	1.96ProSer: 1.96 ± 0.063	1.987ProThr: 1.987 ± 0.057	2.891ProVal: 2.891 ± 0.074	0.526ProTrp: 0.526 ± 0.033	1.868ProTyr: 1.868 ± 0.051	0.0ProXaa: 0.0 ± 0.0
Gln	1.384GlnAla: 1.384 ± 0.046	0.119GlnCys: 0.119 ± 0.014	0.716GlnAsp: 0.716 ± 0.035	1.639GlnGlu: 1.639 ± 0.055	0.817GlnPhe: 0.817 ± 0.036	1.18GlnGly: 1.18 ± 0.047	0.287GlnHis: 0.287 ± 0.021	1.88GlnIle: 1.88 ± 0.054	1.732GlnLys: 1.732 ± 0.051	1.963GlnLeu: 1.963 ± 0.052	0.57GlnMet: 0.57 ± 0.03	0.814GlnAsn: 0.814 ± 0.036	0.66GlnPro: 0.66 ± 0.031	0.499GlnGln: 0.499 ± 0.03	1.241GlnArg: 1.241 ± 0.046	0.855GlnSer: 0.855 ± 0.043	0.917GlnThr: 0.917 ± 0.041	1.406GlnVal: 1.406 ± 0.053	0.233GlnTrp: 0.233 ± 0.022	0.776GlnTyr: 0.776 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.303ArgAla: 3.303 ± 0.076	0.326ArgCys: 0.326 ± 0.022	2.472ArgAsp: 2.472 ± 0.062	5.474ArgGlu: 5.474 ± 0.093	2.256ArgPhe: 2.256 ± 0.061	3.509ArgGly: 3.509 ± 0.076	0.762ArgHis: 0.762 ± 0.039	4.584ArgIle: 4.584 ± 0.091	4.706ArgLys: 4.706 ± 0.104	4.456ArgLeu: 4.456 ± 0.095	1.181ArgMet: 1.181 ± 0.038	1.761ArgAsn: 1.761 ± 0.049	1.507ArgPro: 1.507 ± 0.048	0.937ArgGln: 0.937 ± 0.037	3.064ArgArg: 3.064 ± 0.084	1.766ArgSer: 1.766 ± 0.057	1.97ArgThr: 1.97 ± 0.057	3.7ArgVal: 3.7 ± 0.081	0.608ArgTrp: 0.608 ± 0.032	2.147ArgTyr: 2.147 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	3.145SerAla: 3.145 ± 0.082	0.298SerCys: 0.298 ± 0.027	2.033SerAsp: 2.033 ± 0.055	3.718SerGlu: 3.718 ± 0.086	2.597SerPhe: 2.597 ± 0.072	3.624SerGly: 3.624 ± 0.085	0.781SerHis: 0.781 ± 0.036	3.998SerIle: 3.998 ± 0.091	3.624SerLys: 3.624 ± 0.081	5.46SerLeu: 5.46 ± 0.093	1.134SerMet: 1.134 ± 0.04	1.588SerAsn: 1.588 ± 0.056	2.226SerPro: 2.226 ± 0.066	1.148SerGln: 1.148 ± 0.045	2.279SerArg: 2.279 ± 0.067	2.556SerSer: 2.556 ± 0.08	2.217SerThr: 2.217 ± 0.075	3.135SerVal: 3.135 ± 0.073	0.644SerTrp: 0.644 ± 0.036	2.03SerTyr: 2.03 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.232ThrAla: 3.232 ± 0.067	0.272ThrCys: 0.272 ± 0.02	1.639ThrAsp: 1.639 ± 0.049	2.524ThrGlu: 2.524 ± 0.066	2.09ThrPhe: 2.09 ± 0.065	3.379ThrGly: 3.379 ± 0.081	0.796ThrHis: 0.796 ± 0.038	3.618ThrIle: 3.618 ± 0.084	2.996ThrLys: 2.996 ± 0.066	4.958ThrLeu: 4.958 ± 0.103	0.975ThrMet: 0.975 ± 0.037	1.441ThrAsn: 1.441 ± 0.049	2.405ThrPro: 2.405 ± 0.062	1.053ThrGln: 1.053 ± 0.046	1.81ThrArg: 1.81 ± 0.054	2.204ThrSer: 2.204 ± 0.067	2.388ThrThr: 2.388 ± 0.088	3.472ThrVal: 3.472 ± 0.095	0.538ThrTrp: 0.538 ± 0.032	1.766ThrTyr: 1.766 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	5.01ValAla: 5.01 ± 0.078	0.439ValCys: 0.439 ± 0.029	3.781ValAsp: 3.781 ± 0.076	6.37ValGlu: 6.37 ± 0.112	3.531ValPhe: 3.531 ± 0.077	4.993ValGly: 4.993 ± 0.098	1.205ValHis: 1.205 ± 0.046	6.266ValIle: 6.266 ± 0.086	6.334ValLys: 6.334 ± 0.108	7.463ValLeu: 7.463 ± 0.096	1.645ValMet: 1.645 ± 0.06	2.494ValAsn: 2.494 ± 0.07	3.2ValPro: 3.2 ± 0.082	1.401ValGln: 1.401 ± 0.045	3.708ValArg: 3.708 ± 0.086	3.981ValSer: 3.981 ± 0.082	3.172ValThr: 3.172 ± 0.097	5.892ValVal: 5.892 ± 0.107	0.842ValTrp: 0.842 ± 0.039	3.241ValTyr: 3.241 ± 0.066	0.0ValXaa: 0.0 ± 0.0
Trp	0.795TrpAla: 0.795 ± 0.035	0.048TrpCys: 0.048 ± 0.008	0.679TrpAsp: 0.679 ± 0.035	1.143TrpGlu: 1.143 ± 0.048	0.492TrpPhe: 0.492 ± 0.031	0.8TrpGly: 0.8 ± 0.037	0.238TrpHis: 0.238 ± 0.017	0.972TrpIle: 0.972 ± 0.04	1.024TrpLys: 1.024 ± 0.043	1.15TrpLeu: 1.15 ± 0.044	0.288TrpMet: 0.288 ± 0.023	0.494TrpAsn: 0.494 ± 0.028	0.272TrpPro: 0.272 ± 0.025	0.307TrpGln: 0.307 ± 0.025	0.682TrpArg: 0.682 ± 0.029	0.584TrpSer: 0.584 ± 0.027	0.437TrpThr: 0.437 ± 0.025	0.804TrpVal: 0.804 ± 0.036	0.225TrpTrp: 0.225 ± 0.019	0.439TrpTyr: 0.439 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.806TyrAla: 2.806 ± 0.084	0.282TyrCys: 0.282 ± 0.023	2.035TyrAsp: 2.035 ± 0.061	3.202TyrGlu: 3.202 ± 0.073	2.044TyrPhe: 2.044 ± 0.054	3.121TyrGly: 3.121 ± 0.077	0.673TyrHis: 0.673 ± 0.032	3.043TyrIle: 3.043 ± 0.071	2.516TyrLys: 2.516 ± 0.062	4.592TyrLeu: 4.592 ± 0.098	0.812TyrMet: 0.812 ± 0.034	1.473TyrAsn: 1.473 ± 0.053	1.818TyrPro: 1.818 ± 0.056	0.891TyrGln: 0.891 ± 0.037	1.925TyrArg: 1.925 ± 0.048	2.301TyrSer: 2.301 ± 0.067	1.775TyrThr: 1.775 ± 0.062	2.793TyrVal: 2.793 ± 0.062	0.581TyrTrp: 0.581 ± 0.034	2.006TyrTyr: 2.006 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2265 proteins (631540 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---