Okra leaf curl alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; unclassified Begomovirus-associated alphasatellites

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q6HA18|Q6HA18_9VIRU Replication associated protein OS=Okra leaf curl alphasatellite OX=518829 GN=rep PE=4 SV=1
MM1 pKa = 7.51AAIKK5 pKa = 8.9SHH7 pKa = 5.29WWCFTVFFLSATAPDD22 pKa = 4.4LVPLFEE28 pKa = 4.5NTHH31 pKa = 4.91VSYY34 pKa = 11.22ACWQEE39 pKa = 4.16EE40 pKa = 4.12EE41 pKa = 4.64SPTTKK46 pKa = 9.77RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.72LQGYY53 pKa = 8.29LQLKK57 pKa = 7.47GQRR60 pKa = 11.84TLNQVKK66 pKa = 10.54AIFGDD71 pKa = 4.27LKK73 pKa = 10.89PHH75 pKa = 6.98LEE77 pKa = 3.93KK78 pKa = 10.73QRR80 pKa = 11.84ARR82 pKa = 11.84KK83 pKa = 7.83TDD85 pKa = 3.47DD86 pKa = 3.17ARR88 pKa = 11.84DD89 pKa = 3.56YY90 pKa = 11.49CMKK93 pKa = 10.46PEE95 pKa = 4.08TRR97 pKa = 11.84VSGPFEE103 pKa = 4.09FGEE106 pKa = 4.23YY107 pKa = 10.21CPAGSHH113 pKa = 6.29KK114 pKa = 10.13RR115 pKa = 11.84RR116 pKa = 11.84QRR118 pKa = 11.84EE119 pKa = 3.79LVIRR123 pKa = 11.84SPVRR127 pKa = 11.84MAEE130 pKa = 4.07EE131 pKa = 4.09NPSVFRR137 pKa = 11.84RR138 pKa = 11.84VKK140 pKa = 10.56AKK142 pKa = 9.83IAEE145 pKa = 4.16EE146 pKa = 4.03EE147 pKa = 4.28FQKK150 pKa = 10.7SAHH153 pKa = 6.71EE154 pKa = 3.97IQISNLKK161 pKa = 9.56SWQSRR166 pKa = 11.84LKK168 pKa = 9.88TLLEE172 pKa = 4.45RR173 pKa = 11.84DD174 pKa = 3.44PDD176 pKa = 3.66DD177 pKa = 4.48RR178 pKa = 11.84TILWVYY184 pKa = 9.65GPNGGEE190 pKa = 4.23GKK192 pKa = 8.48STFARR197 pKa = 11.84DD198 pKa = 3.99LYY200 pKa = 11.15RR201 pKa = 11.84SGSWFYY207 pKa = 11.09TRR209 pKa = 11.84GGSADD214 pKa = 3.36NVAYY218 pKa = 10.18QYY220 pKa = 10.65IGCLGNNIVFDD231 pKa = 3.73IPRR234 pKa = 11.84DD235 pKa = 3.53KK236 pKa = 11.12KK237 pKa = 11.08EE238 pKa = 3.7YY239 pKa = 9.84LQYY242 pKa = 11.63SLIEE246 pKa = 4.03MFKK249 pKa = 11.15DD250 pKa = 3.29RR251 pKa = 11.84LIVSNKK257 pKa = 9.63YY258 pKa = 9.28EE259 pKa = 4.2PLMAPLLNCIHH270 pKa = 6.03VVVMSNFLPDD280 pKa = 3.77FEE282 pKa = 6.62KK283 pKa = 10.37ISQDD287 pKa = 3.06RR288 pKa = 11.84VHH290 pKa = 7.24VIPCIPCGVCLKK302 pKa = 10.21HH303 pKa = 7.4HH304 pKa = 6.95NINDD308 pKa = 3.54KK309 pKa = 11.14CDD311 pKa = 3.97DD312 pKa = 3.88YY313 pKa = 12.22VDD315 pKa = 3.42

Molecular weight:
36.58 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q6HA18|Q6HA18_9VIRU Replication associated protein OS=Okra leaf curl alphasatellite OX=518829 GN=rep PE=4 SV=1
MM1 pKa = 7.51AAIKK5 pKa = 8.9SHH7 pKa = 5.29WWCFTVFFLSATAPDD22 pKa = 4.4LVPLFEE28 pKa = 4.5NTHH31 pKa = 4.91VSYY34 pKa = 11.22ACWQEE39 pKa = 4.16EE40 pKa = 4.12EE41 pKa = 4.64SPTTKK46 pKa = 9.77RR47 pKa = 11.84RR48 pKa = 11.84HH49 pKa = 4.72LQGYY53 pKa = 8.29LQLKK57 pKa = 7.47GQRR60 pKa = 11.84TLNQVKK66 pKa = 10.54AIFGDD71 pKa = 4.27LKK73 pKa = 10.89PHH75 pKa = 6.98LEE77 pKa = 3.93KK78 pKa = 10.73QRR80 pKa = 11.84ARR82 pKa = 11.84KK83 pKa = 7.83TDD85 pKa = 3.47DD86 pKa = 3.17ARR88 pKa = 11.84DD89 pKa = 3.56YY90 pKa = 11.49CMKK93 pKa = 10.46PEE95 pKa = 4.08TRR97 pKa = 11.84VSGPFEE103 pKa = 4.09FGEE106 pKa = 4.23YY107 pKa = 10.21CPAGSHH113 pKa = 6.29KK114 pKa = 10.13RR115 pKa = 11.84RR116 pKa = 11.84QRR118 pKa = 11.84EE119 pKa = 3.79LVIRR123 pKa = 11.84SPVRR127 pKa = 11.84MAEE130 pKa = 4.07EE131 pKa = 4.09NPSVFRR137 pKa = 11.84RR138 pKa = 11.84VKK140 pKa = 10.56AKK142 pKa = 9.83IAEE145 pKa = 4.16EE146 pKa = 4.03EE147 pKa = 4.28FQKK150 pKa = 10.7SAHH153 pKa = 6.71EE154 pKa = 3.97IQISNLKK161 pKa = 9.56SWQSRR166 pKa = 11.84LKK168 pKa = 9.88TLLEE172 pKa = 4.45RR173 pKa = 11.84DD174 pKa = 3.44PDD176 pKa = 3.66DD177 pKa = 4.48RR178 pKa = 11.84TILWVYY184 pKa = 9.65GPNGGEE190 pKa = 4.23GKK192 pKa = 8.48STFARR197 pKa = 11.84DD198 pKa = 3.99LYY200 pKa = 11.15RR201 pKa = 11.84SGSWFYY207 pKa = 11.09TRR209 pKa = 11.84GGSADD214 pKa = 3.36NVAYY218 pKa = 10.18QYY220 pKa = 10.65IGCLGNNIVFDD231 pKa = 3.73IPRR234 pKa = 11.84DD235 pKa = 3.53KK236 pKa = 11.12KK237 pKa = 11.08EE238 pKa = 3.7YY239 pKa = 9.84LQYY242 pKa = 11.63SLIEE246 pKa = 4.03MFKK249 pKa = 11.15DD250 pKa = 3.29RR251 pKa = 11.84LIVSNKK257 pKa = 9.63YY258 pKa = 9.28EE259 pKa = 4.2PLMAPLLNCIHH270 pKa = 6.03VVVMSNFLPDD280 pKa = 3.77FEE282 pKa = 6.62KK283 pKa = 10.37ISQDD287 pKa = 3.06RR288 pKa = 11.84VHH290 pKa = 7.24VIPCIPCGVCLKK302 pKa = 10.21HH303 pKa = 7.4HH304 pKa = 6.95NINDD308 pKa = 3.54KK309 pKa = 11.14CDD311 pKa = 3.97DD312 pKa = 3.88YY313 pKa = 12.22VDD315 pKa = 3.42

Molecular weight:
36.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

315

315

315

315.0

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.397 ± 0.0

3.175 ± 0.0

6.032 ± 0.0

6.667 ± 0.0

4.762 ± 0.0

5.079 ± 0.0

3.175 ± 0.0

5.397 ± 0.0

6.984 ± 0.0

7.937 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.0

4.127 ± 0.0

5.397 ± 0.0

4.127 ± 0.0

7.302 ± 0.0

6.349 ± 0.0

3.81 ± 0.0

6.349 ± 0.0

1.905 ± 0.0

4.127 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski