Streptococcus phage Javan249

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A4V4|A0A4D6A4V4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan249 OX=2548070 GN=Javan249_0045 PE=4 SV=1
MM1 pKa = 7.44SVTITTGKK9 pKa = 9.42PIVGSKK15 pKa = 9.75IFYY18 pKa = 9.85FIQSVHH24 pKa = 6.14AKK26 pKa = 9.89IGDD29 pKa = 4.01PATLPAYY36 pKa = 8.68RR37 pKa = 11.84TDD39 pKa = 3.11GTTTLGGEE47 pKa = 4.29YY48 pKa = 9.95LDD50 pKa = 4.65EE51 pKa = 4.0QTQQGRR57 pKa = 11.84LLEE60 pKa = 4.38KK61 pKa = 10.24STDD64 pKa = 3.0EE65 pKa = 4.02HH66 pKa = 6.29TIEE69 pKa = 4.12LTTYY73 pKa = 8.75FAPTDD78 pKa = 3.83PSVKK82 pKa = 9.63TIEE85 pKa = 4.3DD86 pKa = 3.37ASRR89 pKa = 11.84SGDD92 pKa = 3.62SIKK95 pKa = 9.99IWEE98 pKa = 4.56VIVDD102 pKa = 3.94EE103 pKa = 4.69SVKK106 pKa = 9.35TQNEE110 pKa = 4.17EE111 pKa = 3.83QLDD114 pKa = 4.01VYY116 pKa = 9.61PAKK119 pKa = 10.48FGYY122 pKa = 10.64AKK124 pKa = 9.9IGEE127 pKa = 4.37IEE129 pKa = 4.27RR130 pKa = 11.84SAGTTDD136 pKa = 3.82FVEE139 pKa = 4.38MSYY142 pKa = 10.47EE143 pKa = 4.02ANIVGALKK151 pKa = 10.63DD152 pKa = 3.64GQFPLTEE159 pKa = 4.36KK160 pKa = 10.54EE161 pKa = 4.22VALLNSVYY169 pKa = 10.48DD170 pKa = 3.75YY171 pKa = 11.32QNPGEE176 pKa = 4.3TTGDD180 pKa = 3.29YY181 pKa = 11.34DD182 pKa = 6.34SITKK186 pKa = 9.36QQDD189 pKa = 2.53

Molecular weight: 20.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A5U3|A0A4D6A5U3_9CAUD Peptidase_M78 domain-containing protein OS=Streptococcus phage Javan249 OX=2548070 GN=Javan249_0004 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84PNRR5 pKa = 11.84WPYY8 pKa = 9.07SKK10 pKa = 10.18KK11 pKa = 10.45RR12 pKa = 11.84PVFKK16 pKa = 10.21PAVLNSSNVKK26 pKa = 10.48SKK28 pKa = 9.04ITFKK32 pKa = 11.01NNTVIIEE39 pKa = 4.19AQSITGVV46 pKa = 3.19

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12535

43

1481

241.1

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.595 ± 0.718

0.463 ± 0.115

6.199 ± 0.397

6.741 ± 0.492

3.805 ± 0.162

6.223 ± 0.384

1.38 ± 0.176

6.661 ± 0.251

8.137 ± 0.431

8.432 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.27

6.031 ± 0.264

2.68 ± 0.251

4.332 ± 0.267

4.204 ± 0.279

7.204 ± 0.646

6.534 ± 0.423

6.103 ± 0.268

1.117 ± 0.147

3.686 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski