Picobirnavirus Equ3

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus

Average proteome isoelectric point is 5.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4ATH8|A0A0H4ATH8_9VIRU ORF1 OS=Picobirnavirus Equ3 OX=1673646 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.74GEE4 pKa = 3.92LTMANNNNHH13 pKa = 5.75SKK15 pKa = 10.17NRR17 pKa = 11.84RR18 pKa = 11.84GNEE21 pKa = 3.55EE22 pKa = 3.91NEE24 pKa = 4.37EE25 pKa = 4.01KK26 pKa = 10.6RR27 pKa = 11.84SSKK30 pKa = 9.66KK31 pKa = 10.29DD32 pKa = 3.06KK33 pKa = 10.39RR34 pKa = 11.84ISYY37 pKa = 9.57NAYY40 pKa = 9.13KK41 pKa = 10.48SKK43 pKa = 10.85PNDD46 pKa = 3.54PSWYY50 pKa = 6.73EE51 pKa = 3.87TTPEE55 pKa = 3.72LTKK58 pKa = 10.9AVTNFSFDD66 pKa = 3.15TATGYY71 pKa = 11.35AFGMGDD77 pKa = 4.73LDD79 pKa = 5.14DD80 pKa = 5.1PMHH83 pKa = 6.05GTSNPGIMTIAVSPTVGWATNPNSPVNNAAMHH115 pKa = 5.86VYY117 pKa = 10.63SFIRR121 pKa = 11.84HH122 pKa = 5.29EE123 pKa = 4.0NSGNKK128 pKa = 10.03NYY130 pKa = 10.69DD131 pKa = 3.32PNDD134 pKa = 3.25YY135 pKa = 10.67FLYY138 pKa = 10.51LLSYY142 pKa = 10.71DD143 pKa = 4.76GISMWLEE150 pKa = 3.44FLKK153 pKa = 10.51RR154 pKa = 11.84VYY156 pKa = 10.59GVANTYY162 pKa = 10.06HH163 pKa = 6.63PHH165 pKa = 6.01NEE167 pKa = 3.94YY168 pKa = 10.37FPRR171 pKa = 11.84AVLTAMGINAKK182 pKa = 10.3DD183 pKa = 3.59IIKK186 pKa = 10.31HH187 pKa = 5.16KK188 pKa = 10.34PRR190 pKa = 11.84LLTLINTTALDD201 pKa = 3.91LSLKK205 pKa = 10.05KK206 pKa = 10.82VPAYY210 pKa = 10.43LPYY213 pKa = 9.95GAKK216 pKa = 10.02HH217 pKa = 5.42RR218 pKa = 11.84WLMEE222 pKa = 3.56GVYY225 pKa = 10.38QDD227 pKa = 4.49ANLAKK232 pKa = 8.78AQVYY236 pKa = 10.31AFVPEE241 pKa = 4.42YY242 pKa = 10.42IFEE245 pKa = 4.1YY246 pKa = 10.85DD247 pKa = 3.27IDD249 pKa = 4.17EE250 pKa = 5.13DD251 pKa = 4.21GAGCVVPKK259 pKa = 10.25PFYY262 pKa = 10.79SQALALGGYY271 pKa = 9.65GIEE274 pKa = 4.17EE275 pKa = 4.91LEE277 pKa = 4.41TFTQNLMDD285 pKa = 3.61SFFRR289 pKa = 11.84EE290 pKa = 3.48EE291 pKa = 4.25DD292 pKa = 3.5LGVMSGDD299 pKa = 3.16TMKK302 pKa = 10.76AYY304 pKa = 10.29GSNCYY309 pKa = 10.54APMGIEE315 pKa = 3.46STYY318 pKa = 10.87RR319 pKa = 11.84VLPSYY324 pKa = 10.6LPEE327 pKa = 4.34VLDD330 pKa = 4.16QIQNLTLMGGYY341 pKa = 10.16DD342 pKa = 3.6GATCRR347 pKa = 11.84VVQSEE352 pKa = 4.24DD353 pKa = 3.0KK354 pKa = 10.59DD355 pKa = 3.99YY356 pKa = 11.48LVSKK360 pKa = 9.51PEE362 pKa = 3.43WRR364 pKa = 11.84LPEE367 pKa = 4.17EE368 pKa = 4.0YY369 pKa = 9.83RR370 pKa = 11.84AGGRR374 pKa = 11.84TPFMRR379 pKa = 11.84EE380 pKa = 3.17QFLNFEE386 pKa = 4.38MAAVNHH392 pKa = 6.26GNVAEE397 pKa = 4.22ATRR400 pKa = 11.84MTNIASVYY408 pKa = 10.53NEE410 pKa = 4.01VEE412 pKa = 3.92NTIAATTIGSEE423 pKa = 4.09VANYY427 pKa = 9.91AVIWTFGYY435 pKa = 10.67NEE437 pKa = 4.91NIGQFDD443 pKa = 3.92LHH445 pKa = 5.84RR446 pKa = 11.84TPAIFTSFLAPIKK459 pKa = 10.36ADD461 pKa = 3.46SGHH464 pKa = 7.07SLMGMIDD471 pKa = 3.58ADD473 pKa = 3.89VVVNALQPFMYY484 pKa = 9.83DD485 pKa = 4.42LSVLCQFDD493 pKa = 3.81RR494 pKa = 11.84HH495 pKa = 4.95PQIYY499 pKa = 7.74ATAYY503 pKa = 9.74VSYY506 pKa = 9.77PNPDD510 pKa = 3.62DD511 pKa = 4.93PEE513 pKa = 4.67TDD515 pKa = 3.29VTKK518 pKa = 10.15WVYY521 pKa = 10.92ARR523 pKa = 11.84NFLGDD528 pKa = 3.67LANYY532 pKa = 9.04YY533 pKa = 10.82VIDD536 pKa = 3.76RR537 pKa = 11.84TEE539 pKa = 4.51LEE541 pKa = 4.24LLSTNALKK549 pKa = 11.03AEE551 pKa = 4.46FGLMTNN557 pKa = 4.23

Molecular weight: 62.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4AQ74|A0A0H4AQ74_9VIRU Capsid protein OS=Picobirnavirus Equ3 OX=1673646 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.14IEE4 pKa = 4.27SLPSEE9 pKa = 4.59VYY11 pKa = 10.59DD12 pKa = 4.03VVLNNDD18 pKa = 3.75GLKK21 pKa = 9.66MYY23 pKa = 10.67LNNLEE28 pKa = 4.49RR29 pKa = 11.84GRR31 pKa = 11.84SATPRR36 pKa = 11.84SWLYY40 pKa = 10.04EE41 pKa = 4.04GKK43 pKa = 10.17SDD45 pKa = 5.04TEE47 pKa = 4.49VLQAWLTILEE57 pKa = 4.51TVKK60 pKa = 10.74SSEE63 pKa = 4.11FGEE66 pKa = 5.05LIYY69 pKa = 10.9QFDD72 pKa = 3.78TSQLKK77 pKa = 9.76KK78 pKa = 9.56WGAQGEE84 pKa = 4.47VKK86 pKa = 10.18PIKK89 pKa = 10.07EE90 pKa = 3.87LMEE93 pKa = 4.07IVTEE97 pKa = 4.31GFTNAGTPKK106 pKa = 9.5PAIWNTPMWQQAKK119 pKa = 9.24TNAIKK124 pKa = 10.83YY125 pKa = 9.04LIIDD129 pKa = 3.5TGLYY133 pKa = 9.75KK134 pKa = 10.54RR135 pKa = 11.84LRR137 pKa = 11.84PRR139 pKa = 11.84ALSHH143 pKa = 6.55VVDD146 pKa = 4.82DD147 pKa = 3.95MRR149 pKa = 11.84DD150 pKa = 3.47RR151 pKa = 11.84DD152 pKa = 4.13TLEE155 pKa = 4.43SNSGFPDD162 pKa = 4.05FGRR165 pKa = 11.84RR166 pKa = 11.84KK167 pKa = 9.98KK168 pKa = 10.69PEE170 pKa = 3.57ILTAAFEE177 pKa = 4.74AIKK180 pKa = 10.74SGEE183 pKa = 3.93FWEE186 pKa = 5.61FPAIVLFRR194 pKa = 11.84NYY196 pKa = 8.69NQKK199 pKa = 8.3TRR201 pKa = 11.84IVWMYY206 pKa = 10.73PMATNIYY213 pKa = 8.93EE214 pKa = 4.42GSFTQPLKK222 pKa = 9.86EE223 pKa = 4.19ALMKK227 pKa = 10.56SDD229 pKa = 5.31LDD231 pKa = 5.55FIAPWRR237 pKa = 11.84GYY239 pKa = 10.15DD240 pKa = 3.28HH241 pKa = 7.0VLARR245 pKa = 11.84LSKK248 pKa = 10.72LYY250 pKa = 9.29GTGEE254 pKa = 4.12FLSASDD260 pKa = 4.72FSHH263 pKa = 6.94TDD265 pKa = 2.65AHH267 pKa = 5.21FTKK270 pKa = 10.04WAILEE275 pKa = 4.23VYY277 pKa = 10.29DD278 pKa = 3.89VIKK281 pKa = 10.93YY282 pKa = 9.78AFQEE286 pKa = 4.38QYY288 pKa = 9.78WDD290 pKa = 3.46EE291 pKa = 4.48LKK293 pKa = 10.81RR294 pKa = 11.84SMLHH298 pKa = 5.01VVEE301 pKa = 4.72IPLIIGEE308 pKa = 4.2NSWIIGDD315 pKa = 4.19HH316 pKa = 5.76GVSSGSNWTNDD327 pKa = 2.56VEE329 pKa = 4.67TYY331 pKa = 9.62MDD333 pKa = 5.01FIIAQYY339 pKa = 9.06LTLLKK344 pKa = 10.35LVKK347 pKa = 9.24EE348 pKa = 4.19PCMAIGDD355 pKa = 4.77DD356 pKa = 3.27ISHH359 pKa = 6.65RR360 pKa = 11.84RR361 pKa = 11.84DD362 pKa = 3.1SYY364 pKa = 11.79LPDD367 pKa = 4.34LDD369 pKa = 3.97EE370 pKa = 6.23QIAQVCQSMGFDD382 pKa = 3.43VNAEE386 pKa = 4.15KK387 pKa = 9.95VTNQQDD393 pKa = 3.24WVKK396 pKa = 10.1FLQRR400 pKa = 11.84LTIRR404 pKa = 11.84GFNSEE409 pKa = 3.49RR410 pKa = 11.84TYY412 pKa = 11.0SVEE415 pKa = 3.71GVEE418 pKa = 4.25YY419 pKa = 9.74PLLRR423 pKa = 11.84GIYY426 pKa = 8.39STIRR430 pKa = 11.84ALNSSLNPEE439 pKa = 4.47KK440 pKa = 10.57FHH442 pKa = 7.59SPKK445 pKa = 10.38LWSKK449 pKa = 11.74DD450 pKa = 3.28MFAVRR455 pKa = 11.84QFMILEE461 pKa = 4.07NCIDD465 pKa = 3.82HH466 pKa = 6.94PLFVQFVKK474 pKa = 9.7FVCAGNPYY482 pKa = 10.04LVKK485 pKa = 10.2FAKK488 pKa = 10.29LKK490 pKa = 10.9NEE492 pKa = 4.15QIDD495 pKa = 4.1KK496 pKa = 10.56KK497 pKa = 9.1WAQSRR502 pKa = 11.84ILPGLNPTYY511 pKa = 10.41NQEE514 pKa = 4.26KK515 pKa = 9.62RR516 pKa = 11.84DD517 pKa = 4.09KK518 pKa = 10.65PLSTYY523 pKa = 10.79AAIAIARR530 pKa = 11.84SLL532 pKa = 3.55

Molecular weight: 61.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1340

251

557

446.7

50.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.955 ± 2.272

0.597 ± 0.184

5.373 ± 0.672

6.716 ± 0.162

3.881 ± 0.971

6.642 ± 1.23

2.015 ± 0.181

5.224 ± 0.825

5.299 ± 0.909

7.985 ± 1.121

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.985 ± 0.44

6.716 ± 1.269

4.925 ± 0.256

3.507 ± 0.755

4.478 ± 0.382

6.194 ± 0.351

5.97 ± 0.401

5.448 ± 0.583

2.015 ± 0.455

5.075 ± 0.87
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski