Amino acid dipepetide frequency for Gordonia phage Benczkowski14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.5AlaAla: 10.5 ± 1.905
0.717AlaCys: 0.717 ± 0.214
5.566AlaAsp: 5.566 ± 0.468
5.102AlaGlu: 5.102 ± 0.503
3.669AlaPhe: 3.669 ± 0.429
7.295AlaGly: 7.295 ± 0.979
1.434AlaHis: 1.434 ± 0.281
5.524AlaIle: 5.524 ± 0.726
4.849AlaLys: 4.849 ± 0.578
8.054AlaLeu: 8.054 ± 1.608
2.825AlaMet: 2.825 ± 0.743
3.163AlaAsn: 3.163 ± 0.506
4.512AlaPro: 4.512 ± 0.5
3.837AlaGln: 3.837 ± 0.435
5.566AlaArg: 5.566 ± 0.492
4.554AlaSer: 4.554 ± 0.437
5.018AlaThr: 5.018 ± 0.475
6.41AlaVal: 6.41 ± 0.643
1.687AlaTrp: 1.687 ± 0.548
2.277AlaTyr: 2.277 ± 0.303
0.0AlaXaa: 0.0 ± 0.0
Cys
0.464CysAla: 0.464 ± 0.163
0.127CysCys: 0.127 ± 0.086
0.675CysAsp: 0.675 ± 0.198
0.506CysGlu: 0.506 ± 0.138
0.295CysPhe: 0.295 ± 0.122
0.843CysGly: 0.843 ± 0.215
0.253CysHis: 0.253 ± 0.113
0.464CysIle: 0.464 ± 0.149
0.548CysLys: 0.548 ± 0.163
0.717CysLeu: 0.717 ± 0.185
0.169CysMet: 0.169 ± 0.086
0.464CysAsn: 0.464 ± 0.146
0.717CysPro: 0.717 ± 0.19
0.169CysGln: 0.169 ± 0.082
0.675CysArg: 0.675 ± 0.195
0.675CysSer: 0.675 ± 0.194
0.717CysThr: 0.717 ± 0.198
0.59CysVal: 0.59 ± 0.201
0.38CysTrp: 0.38 ± 0.135
0.295CysTyr: 0.295 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
5.271AspAla: 5.271 ± 0.606
0.337AspCys: 0.337 ± 0.131
5.313AspAsp: 5.313 ± 0.974
5.187AspGlu: 5.187 ± 0.753
2.361AspPhe: 2.361 ± 0.317
5.271AspGly: 5.271 ± 0.574
1.518AspHis: 1.518 ± 0.255
2.868AspIle: 2.868 ± 0.38
2.825AspLys: 2.825 ± 0.29
4.596AspLeu: 4.596 ± 0.444
1.518AspMet: 1.518 ± 0.227
1.855AspAsn: 1.855 ± 0.256
4.892AspPro: 4.892 ± 0.618
1.771AspGln: 1.771 ± 0.277
3.416AspArg: 3.416 ± 0.451
4.343AspSer: 4.343 ± 0.317
2.868AspThr: 2.868 ± 0.378
3.964AspVal: 3.964 ± 0.443
1.139AspTrp: 1.139 ± 0.263
2.53AspTyr: 2.53 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
5.735GluAla: 5.735 ± 0.558
0.506GluCys: 0.506 ± 0.158
4.217GluAsp: 4.217 ± 0.54
4.892GluGlu: 4.892 ± 0.698
2.446GluPhe: 2.446 ± 0.345
4.512GluGly: 4.512 ± 0.382
1.307GluHis: 1.307 ± 0.271
4.006GluIle: 4.006 ± 0.444
3.121GluLys: 3.121 ± 0.461
6.072GluLeu: 6.072 ± 0.476
2.488GluMet: 2.488 ± 0.369
2.361GluAsn: 2.361 ± 0.393
3.5GluPro: 3.5 ± 0.617
2.783GluGln: 2.783 ± 0.357
4.133GluArg: 4.133 ± 0.627
3.964GluSer: 3.964 ± 0.259
3.922GluThr: 3.922 ± 0.519
5.018GluVal: 5.018 ± 0.565
1.434GluTrp: 1.434 ± 0.229
2.614GluTyr: 2.614 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
3.205PheAla: 3.205 ± 0.594
0.506PheCys: 0.506 ± 0.138
2.614PheAsp: 2.614 ± 0.296
2.825PheGlu: 2.825 ± 0.453
1.223PhePhe: 1.223 ± 0.298
2.614PheGly: 2.614 ± 0.352
0.717PheHis: 0.717 ± 0.196
1.392PheIle: 1.392 ± 0.252
1.813PheLys: 1.813 ± 0.419
2.151PheLeu: 2.151 ± 0.435
1.096PheMet: 1.096 ± 0.271
1.602PheAsn: 1.602 ± 0.211
1.56PhePro: 1.56 ± 0.33
1.434PheGln: 1.434 ± 0.249
1.855PheArg: 1.855 ± 0.286
2.235PheSer: 2.235 ± 0.285
2.235PheThr: 2.235 ± 0.274
2.614PheVal: 2.614 ± 0.246
0.464PheTrp: 0.464 ± 0.122
0.886PheTyr: 0.886 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
6.789GlyAla: 6.789 ± 0.976
0.801GlyCys: 0.801 ± 0.258
4.343GlyAsp: 4.343 ± 0.434
4.596GlyGlu: 4.596 ± 0.37
3.205GlyPhe: 3.205 ± 0.447
5.819GlyGly: 5.819 ± 0.57
1.392GlyHis: 1.392 ± 0.286
4.006GlyIle: 4.006 ± 0.653
5.187GlyLys: 5.187 ± 0.447
5.102GlyLeu: 5.102 ± 0.506
3.036GlyMet: 3.036 ± 0.603
2.741GlyAsn: 2.741 ± 0.411
3.542GlyPro: 3.542 ± 0.352
2.994GlyGln: 2.994 ± 0.377
4.048GlyArg: 4.048 ± 0.447
4.934GlySer: 4.934 ± 0.507
4.849GlyThr: 4.849 ± 0.424
5.06GlyVal: 5.06 ± 0.402
1.349GlyTrp: 1.349 ± 0.244
2.614GlyTyr: 2.614 ± 0.334
0.0GlyXaa: 0.0 ± 0.0
His
1.349HisAla: 1.349 ± 0.253
0.211HisCys: 0.211 ± 0.094
0.886HisAsp: 0.886 ± 0.255
2.066HisGlu: 2.066 ± 0.419
0.59HisPhe: 0.59 ± 0.176
1.56HisGly: 1.56 ± 0.317
0.337HisHis: 0.337 ± 0.1
1.054HisIle: 1.054 ± 0.212
1.054HisLys: 1.054 ± 0.216
1.349HisLeu: 1.349 ± 0.285
0.548HisMet: 0.548 ± 0.148
0.38HisAsn: 0.38 ± 0.124
1.392HisPro: 1.392 ± 0.329
0.759HisGln: 0.759 ± 0.207
1.265HisArg: 1.265 ± 0.299
1.307HisSer: 1.307 ± 0.231
1.139HisThr: 1.139 ± 0.202
1.139HisVal: 1.139 ± 0.189
0.337HisTrp: 0.337 ± 0.115
0.843HisTyr: 0.843 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.229IleAla: 5.229 ± 0.591
0.295IleCys: 0.295 ± 0.133
3.5IleAsp: 3.5 ± 0.344
4.554IleGlu: 4.554 ± 0.585
1.855IlePhe: 1.855 ± 0.289
4.807IleGly: 4.807 ± 0.527
1.181IleHis: 1.181 ± 0.241
3.374IleIle: 3.374 ± 0.321
3.416IleLys: 3.416 ± 0.455
3.669IleLeu: 3.669 ± 0.424
1.265IleMet: 1.265 ± 0.172
2.319IleAsn: 2.319 ± 0.245
2.108IlePro: 2.108 ± 0.326
2.277IleGln: 2.277 ± 0.304
2.783IleArg: 2.783 ± 0.318
2.53IleSer: 2.53 ± 0.303
3.711IleThr: 3.711 ± 0.336
2.783IleVal: 2.783 ± 0.537
0.843IleTrp: 0.843 ± 0.252
1.349IleTyr: 1.349 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
5.398LysAla: 5.398 ± 0.468
0.59LysCys: 0.59 ± 0.181
2.783LysAsp: 2.783 ± 0.394
3.374LysGlu: 3.374 ± 0.362
2.277LysPhe: 2.277 ± 0.34
3.247LysGly: 3.247 ± 0.463
1.181LysHis: 1.181 ± 0.263
2.614LysIle: 2.614 ± 0.3
3.205LysLys: 3.205 ± 0.394
4.596LysLeu: 4.596 ± 0.633
1.434LysMet: 1.434 ± 0.228
2.151LysAsn: 2.151 ± 0.28
2.614LysPro: 2.614 ± 0.563
1.855LysGln: 1.855 ± 0.31
3.5LysArg: 3.5 ± 0.493
3.416LysSer: 3.416 ± 0.423
2.572LysThr: 2.572 ± 0.33
3.795LysVal: 3.795 ± 0.441
0.843LysTrp: 0.843 ± 0.156
1.687LysTyr: 1.687 ± 0.335
0.0LysXaa: 0.0 ± 0.0
Leu
6.621LeuAla: 6.621 ± 0.617
1.054LeuCys: 1.054 ± 0.28
5.145LeuAsp: 5.145 ± 0.546
5.609LeuGlu: 5.609 ± 0.485
2.277LeuPhe: 2.277 ± 0.326
5.946LeuGly: 5.946 ± 0.852
1.307LeuHis: 1.307 ± 0.244
3.922LeuIle: 3.922 ± 0.469
3.964LeuLys: 3.964 ± 0.519
4.639LeuLeu: 4.639 ± 0.453
2.151LeuMet: 2.151 ± 0.313
3.078LeuAsn: 3.078 ± 0.338
5.355LeuPro: 5.355 ± 0.586
2.699LeuGln: 2.699 ± 0.34
5.018LeuArg: 5.018 ± 0.605
3.795LeuSer: 3.795 ± 0.473
3.964LeuThr: 3.964 ± 0.46
4.807LeuVal: 4.807 ± 0.458
0.97LeuTrp: 0.97 ± 0.188
1.855LeuTyr: 1.855 ± 0.284
0.0LeuXaa: 0.0 ± 0.0
Met
3.247MetAla: 3.247 ± 0.512
0.169MetCys: 0.169 ± 0.076
1.096MetAsp: 1.096 ± 0.209
1.729MetGlu: 1.729 ± 0.268
0.97MetPhe: 0.97 ± 0.212
1.855MetGly: 1.855 ± 0.23
0.843MetHis: 0.843 ± 0.198
1.223MetIle: 1.223 ± 0.527
1.476MetLys: 1.476 ± 0.253
1.771MetLeu: 1.771 ± 0.407
0.633MetMet: 0.633 ± 0.2
1.518MetAsn: 1.518 ± 0.347
1.56MetPro: 1.56 ± 0.299
1.476MetGln: 1.476 ± 0.502
1.392MetArg: 1.392 ± 0.245
2.277MetSer: 2.277 ± 0.297
2.066MetThr: 2.066 ± 0.243
1.687MetVal: 1.687 ± 0.35
0.337MetTrp: 0.337 ± 0.101
0.675MetTyr: 0.675 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.584AsnAla: 3.584 ± 0.614
0.337AsnCys: 0.337 ± 0.118
1.898AsnAsp: 1.898 ± 0.281
2.193AsnGlu: 2.193 ± 0.254
1.56AsnPhe: 1.56 ± 0.274
3.078AsnGly: 3.078 ± 0.311
0.843AsnHis: 0.843 ± 0.187
2.193AsnIle: 2.193 ± 0.329
2.361AsnLys: 2.361 ± 0.304
2.741AsnLeu: 2.741 ± 0.325
0.717AsnMet: 0.717 ± 0.134
1.94AsnAsn: 1.94 ± 0.283
2.446AsnPro: 2.446 ± 0.274
1.729AsnGln: 1.729 ± 0.266
2.151AsnArg: 2.151 ± 0.338
2.108AsnSer: 2.108 ± 0.3
2.783AsnThr: 2.783 ± 0.339
2.952AsnVal: 2.952 ± 0.381
0.548AsnTrp: 0.548 ± 0.182
1.645AsnTyr: 1.645 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
5.355ProAla: 5.355 ± 0.59
0.295ProCys: 0.295 ± 0.152
4.259ProAsp: 4.259 ± 0.734
5.018ProGlu: 5.018 ± 0.686
1.645ProPhe: 1.645 ± 0.282
4.048ProGly: 4.048 ± 0.42
0.801ProHis: 0.801 ± 0.182
2.952ProIle: 2.952 ± 0.344
2.446ProLys: 2.446 ± 0.52
3.627ProLeu: 3.627 ± 0.426
1.139ProMet: 1.139 ± 0.188
1.94ProAsn: 1.94 ± 0.255
3.163ProPro: 3.163 ± 0.611
1.813ProGln: 1.813 ± 0.253
2.151ProArg: 2.151 ± 0.425
3.289ProSer: 3.289 ± 0.426
4.175ProThr: 4.175 ± 0.557
3.964ProVal: 3.964 ± 0.429
1.054ProTrp: 1.054 ± 0.231
1.476ProTyr: 1.476 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
3.837GlnAla: 3.837 ± 0.624
0.548GlnCys: 0.548 ± 0.213
1.602GlnAsp: 1.602 ± 0.276
1.94GlnGlu: 1.94 ± 0.324
1.307GlnPhe: 1.307 ± 0.22
2.825GlnGly: 2.825 ± 0.408
0.843GlnHis: 0.843 ± 0.165
2.024GlnIle: 2.024 ± 0.295
1.94GlnLys: 1.94 ± 0.243
3.331GlnLeu: 3.331 ± 0.39
1.012GlnMet: 1.012 ± 0.186
1.349GlnAsn: 1.349 ± 0.229
1.898GlnPro: 1.898 ± 0.365
1.434GlnGln: 1.434 ± 0.356
2.868GlnArg: 2.868 ± 0.347
2.108GlnSer: 2.108 ± 0.246
2.024GlnThr: 2.024 ± 0.279
3.078GlnVal: 3.078 ± 0.247
1.096GlnTrp: 1.096 ± 0.284
0.886GlnTyr: 0.886 ± 0.19
0.0GlnXaa: 0.0 ± 0.0
Arg
5.102ArgAla: 5.102 ± 0.475
0.759ArgCys: 0.759 ± 0.208
3.5ArgAsp: 3.5 ± 0.515
3.753ArgGlu: 3.753 ± 0.65
1.771ArgPhe: 1.771 ± 0.259
3.711ArgGly: 3.711 ± 0.48
1.265ArgHis: 1.265 ± 0.279
3.331ArgIle: 3.331 ± 0.516
3.078ArgLys: 3.078 ± 0.428
4.596ArgLeu: 4.596 ± 0.54
2.108ArgMet: 2.108 ± 0.37
2.319ArgAsn: 2.319 ± 0.358
3.121ArgPro: 3.121 ± 0.512
1.771ArgGln: 1.771 ± 0.257
3.374ArgArg: 3.374 ± 0.646
2.699ArgSer: 2.699 ± 0.342
3.331ArgThr: 3.331 ± 0.403
4.343ArgVal: 4.343 ± 0.484
0.928ArgTrp: 0.928 ± 0.205
1.898ArgTyr: 1.898 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
5.06SerAla: 5.06 ± 0.49
0.675SerCys: 0.675 ± 0.221
3.627SerAsp: 3.627 ± 0.506
3.163SerGlu: 3.163 ± 0.463
1.729SerPhe: 1.729 ± 0.289
5.271SerGly: 5.271 ± 0.486
1.054SerHis: 1.054 ± 0.239
3.078SerIle: 3.078 ± 0.456
3.163SerLys: 3.163 ± 0.279
4.639SerLeu: 4.639 ± 0.452
1.645SerMet: 1.645 ± 0.225
2.699SerAsn: 2.699 ± 0.315
3.036SerPro: 3.036 ± 0.457
2.235SerGln: 2.235 ± 0.314
2.614SerArg: 2.614 ± 0.413
3.669SerSer: 3.669 ± 0.407
4.428SerThr: 4.428 ± 0.46
3.753SerVal: 3.753 ± 0.463
0.886SerTrp: 0.886 ± 0.163
1.898SerTyr: 1.898 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
6.452ThrAla: 6.452 ± 0.967
0.38ThrCys: 0.38 ± 0.141
3.88ThrAsp: 3.88 ± 0.489
4.048ThrGlu: 4.048 ± 0.521
1.94ThrPhe: 1.94 ± 0.251
5.187ThrGly: 5.187 ± 0.445
0.886ThrHis: 0.886 ± 0.215
3.078ThrIle: 3.078 ± 0.333
3.584ThrLys: 3.584 ± 0.364
4.512ThrLeu: 4.512 ± 0.434
1.181ThrMet: 1.181 ± 0.227
2.446ThrAsn: 2.446 ± 0.288
3.374ThrPro: 3.374 ± 0.489
2.108ThrGln: 2.108 ± 0.274
3.163ThrArg: 3.163 ± 0.403
3.584ThrSer: 3.584 ± 0.37
3.416ThrThr: 3.416 ± 0.438
4.47ThrVal: 4.47 ± 0.605
1.476ThrTrp: 1.476 ± 0.237
1.982ThrTyr: 1.982 ± 0.414
0.0ThrXaa: 0.0 ± 0.0
Val
5.609ValAla: 5.609 ± 0.655
0.717ValCys: 0.717 ± 0.185
5.018ValAsp: 5.018 ± 0.45
5.145ValGlu: 5.145 ± 0.36
2.235ValPhe: 2.235 ± 0.299
4.807ValGly: 4.807 ± 0.61
1.223ValHis: 1.223 ± 0.265
4.09ValIle: 4.09 ± 0.469
3.374ValLys: 3.374 ± 0.466
4.512ValLeu: 4.512 ± 0.538
1.602ValMet: 1.602 ± 0.192
2.91ValAsn: 2.91 ± 0.282
3.837ValPro: 3.837 ± 0.379
3.374ValGln: 3.374 ± 0.357
3.711ValArg: 3.711 ± 0.462
4.006ValSer: 4.006 ± 0.402
4.386ValThr: 4.386 ± 0.514
4.512ValVal: 4.512 ± 0.549
0.97ValTrp: 0.97 ± 0.261
1.898ValTyr: 1.898 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
1.392TrpAla: 1.392 ± 0.349
0.295TrpCys: 0.295 ± 0.122
1.56TrpAsp: 1.56 ± 0.323
1.096TrpGlu: 1.096 ± 0.194
0.886TrpPhe: 0.886 ± 0.214
1.139TrpGly: 1.139 ± 0.227
0.506TrpHis: 0.506 ± 0.166
0.928TrpIle: 0.928 ± 0.205
0.464TrpLys: 0.464 ± 0.17
1.139TrpLeu: 1.139 ± 0.192
0.675TrpMet: 0.675 ± 0.124
0.886TrpAsn: 0.886 ± 0.24
0.801TrpPro: 0.801 ± 0.155
0.464TrpGln: 0.464 ± 0.114
1.223TrpArg: 1.223 ± 0.21
1.223TrpSer: 1.223 ± 0.222
1.181TrpThr: 1.181 ± 0.216
1.096TrpVal: 1.096 ± 0.207
0.169TrpTrp: 0.169 ± 0.081
0.38TrpTyr: 0.38 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.361TyrAla: 2.361 ± 0.322
0.422TyrCys: 0.422 ± 0.144
2.361TyrAsp: 2.361 ± 0.358
2.235TyrGlu: 2.235 ± 0.364
0.801TyrPhe: 0.801 ± 0.165
2.572TyrGly: 2.572 ± 0.363
0.675TyrHis: 0.675 ± 0.211
1.729TyrIle: 1.729 ± 0.307
1.265TyrLys: 1.265 ± 0.288
2.404TyrLeu: 2.404 ± 0.385
0.717TyrMet: 0.717 ± 0.211
1.687TyrAsn: 1.687 ± 0.199
1.181TyrPro: 1.181 ± 0.235
0.97TyrGln: 0.97 ± 0.207
1.855TyrArg: 1.855 ± 0.309
1.602TyrSer: 1.602 ± 0.284
2.361TyrThr: 2.361 ± 0.292
1.898TyrVal: 1.898 ± 0.299
0.59TyrTrp: 0.59 ± 0.136
0.928TyrTyr: 0.928 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (23715 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski