Roseiconus nitratireducens

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Roseiconus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5599 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M6D3T7|A0A5M6D3T7_9BACT Glycosyl transferase family 25 OS=Roseiconus nitratireducens OX=2605748 GN=FYK55_18440 PE=4 SV=1
MM1 pKa = 7.37FPNPPGRR8 pKa = 11.84FAQSRR13 pKa = 11.84YY14 pKa = 9.03RR15 pKa = 11.84CLTRR19 pKa = 11.84GLEE22 pKa = 4.02NLEE25 pKa = 4.01RR26 pKa = 11.84RR27 pKa = 11.84CLLSFDD33 pKa = 4.2YY34 pKa = 11.42ASMVQVAPGLAVDD47 pKa = 4.1PGDD50 pKa = 3.86YY51 pKa = 11.03EE52 pKa = 4.22EE53 pKa = 5.83GSLLVKK59 pKa = 10.36YY60 pKa = 9.82KK61 pKa = 8.92QTGIGAVRR69 pKa = 11.84VTEE72 pKa = 4.17SLGATVGRR80 pKa = 11.84AYY82 pKa = 9.5TQIPGLHH89 pKa = 4.82QVKK92 pKa = 10.08LPSGVKK98 pKa = 10.0VADD101 pKa = 3.28VLPRR105 pKa = 11.84FRR107 pKa = 11.84NNPNVLYY114 pKa = 10.67AEE116 pKa = 4.5PDD118 pKa = 3.54FRR120 pKa = 11.84LHH122 pKa = 5.27TTALPNDD129 pKa = 3.59PRR131 pKa = 11.84YY132 pKa = 10.83DD133 pKa = 3.96EE134 pKa = 5.42LWGLNNTGQTGGTVDD149 pKa = 4.04ADD151 pKa = 3.66SDD153 pKa = 3.66IAEE156 pKa = 4.44AWDD159 pKa = 3.23IATGSNLVVAVIDD172 pKa = 3.55TGVDD176 pKa = 3.51YY177 pKa = 11.29LHH179 pKa = 7.45EE180 pKa = 4.87DD181 pKa = 3.38LAANMWVNAGEE192 pKa = 4.17IPGDD196 pKa = 4.39GIDD199 pKa = 3.47NDD201 pKa = 4.24GNGFVDD207 pKa = 6.01DD208 pKa = 3.28IHH210 pKa = 8.6GYY212 pKa = 10.82DD213 pKa = 4.63FANDD217 pKa = 4.4DD218 pKa = 4.89PDD220 pKa = 5.01PMDD223 pKa = 4.27DD224 pKa = 3.65QGHH227 pKa = 5.39GTHH230 pKa = 6.09VAGTIGAVGDD240 pKa = 3.65NGIGVVGVNWDD251 pKa = 3.63VQLMAVKK258 pKa = 10.32FLDD261 pKa = 3.39ATGNGTTSDD270 pKa = 3.45AVDD273 pKa = 4.83AINYY277 pKa = 8.56AVANGAQIANASWGGNEE294 pKa = 4.44PFSQALYY301 pKa = 10.63DD302 pKa = 4.79AIAGGRR308 pKa = 11.84DD309 pKa = 2.87AGQIFIAGAGNGNAFGFGQDD329 pKa = 3.09NDD331 pKa = 4.31SNPFYY336 pKa = 10.12PASYY340 pKa = 10.72DD341 pKa = 3.34LDD343 pKa = 4.46NIISVAATDD352 pKa = 4.25HH353 pKa = 6.16NDD355 pKa = 2.87ARR357 pKa = 11.84ATFSNYY363 pKa = 10.51GITSVDD369 pKa = 3.3LAGPGVSILSTRR381 pKa = 11.84PGNSYY386 pKa = 10.5EE387 pKa = 4.16LLSGTSMATPHH398 pKa = 5.53VAGVASLVWQHH409 pKa = 5.67NPTWTADD416 pKa = 3.51QVIEE420 pKa = 4.28QILNSVDD427 pKa = 3.81PVSSMQGITTTGGRR441 pKa = 11.84LNAAAALGEE450 pKa = 4.5PVPPPPPPPPATLPVSEE467 pKa = 5.15DD468 pKa = 3.57FEE470 pKa = 5.11DD471 pKa = 5.33ALAQDD476 pKa = 3.78FQVRR480 pKa = 11.84VGTWGVTSGRR490 pKa = 11.84YY491 pKa = 9.64AITPVMGDD499 pKa = 3.62PSASAVSTVRR509 pKa = 11.84LEE511 pKa = 4.15TPLADD516 pKa = 3.99DD517 pKa = 4.1MEE519 pKa = 4.76VFATANAAPEE529 pKa = 3.66IGFFFNYY536 pKa = 10.01LSNGYY541 pKa = 9.45IVFDD545 pKa = 3.76YY546 pKa = 11.07QDD548 pKa = 3.56NFDD551 pKa = 3.77FKK553 pKa = 10.7YY554 pKa = 10.72AGFNVVSGQWVIGQRR569 pKa = 11.84SGSGWQNLATLSDD582 pKa = 3.54AGIAASTDD590 pKa = 2.59VGMRR594 pKa = 11.84LTIEE598 pKa = 4.92DD599 pKa = 4.09GQSVTLYY606 pKa = 11.01GKK608 pKa = 9.3GAPVLSHH615 pKa = 6.59SFAEE619 pKa = 4.45SVVDD623 pKa = 4.02GQVGVGAQNSQTAFDD638 pKa = 3.68NVYY641 pKa = 10.75VNTFVSATPAALPIQEE657 pKa = 4.96DD658 pKa = 4.29FSDD661 pKa = 3.84GTADD665 pKa = 3.33NFQPQTPGWSVSGGAYY681 pKa = 9.61QVDD684 pKa = 4.24AFTGANAFSTLQVDD698 pKa = 4.71GALPADD704 pKa = 3.76LEE706 pKa = 4.34LRR708 pKa = 11.84ATANVHH714 pKa = 6.1APSGSQSSNALLVFDD729 pKa = 4.61YY730 pKa = 11.21HH731 pKa = 7.85NEE733 pKa = 3.79NDD735 pKa = 3.86FKK737 pKa = 11.17FVAAYY742 pKa = 10.17AGTDD746 pKa = 2.98QWVIGVRR753 pKa = 11.84GAGWTTVAEE762 pKa = 4.05TSEE765 pKa = 4.17AVEE768 pKa = 4.34TNVDD772 pKa = 2.66YY773 pKa = 11.6DD774 pKa = 3.76MQVVIEE780 pKa = 5.08DD781 pKa = 4.09GSSVTLLVDD790 pKa = 3.99GVVKK794 pKa = 9.84ATHH797 pKa = 6.52TFADD801 pKa = 3.9SVTDD805 pKa = 4.02GVLGLGTLNASVHH818 pKa = 6.12FDD820 pKa = 3.38DD821 pKa = 6.62LVVQEE826 pKa = 5.08YY827 pKa = 10.37LPPPPPPPGSLPIVEE842 pKa = 5.0DD843 pKa = 4.41FSDD846 pKa = 3.72GLADD850 pKa = 3.65YY851 pKa = 7.53FQPQLGNWSVNNGQYY866 pKa = 10.33FGSPSGDD873 pKa = 3.09ALSTLNLAEE882 pKa = 4.96ALPSDD887 pKa = 4.06LDD889 pKa = 3.59FRR891 pKa = 11.84ATVAVQQPTSGLYY904 pKa = 9.89QNAVMIFDD912 pKa = 4.0YY913 pKa = 10.94QSPTNFKK920 pKa = 10.41FAGAYY925 pKa = 10.1AGTDD929 pKa = 2.91QWVIGQRR936 pKa = 11.84TSQWVTLASAGAAVDD951 pKa = 3.51VGVDD955 pKa = 3.2YY956 pKa = 11.1DD957 pKa = 3.88LRR959 pKa = 11.84LVIQAGNQVALYY971 pKa = 10.91ANGALQTTYY980 pKa = 11.06AFSGSVTDD988 pKa = 4.04GAIGLGTWNALARR1001 pKa = 11.84FDD1003 pKa = 5.14DD1004 pKa = 4.25VSLQTYY1010 pKa = 7.31VAPPPPPAASLPLSEE1025 pKa = 5.39DD1026 pKa = 3.47FSDD1029 pKa = 4.22GVADD1033 pKa = 4.83FFQPQVGDD1041 pKa = 3.43WLVNAGRR1048 pKa = 11.84YY1049 pKa = 8.5QGSPSGDD1056 pKa = 2.91AVTTLNVAEE1065 pKa = 4.52PLPTEE1070 pKa = 4.15LEE1072 pKa = 4.07FRR1074 pKa = 11.84ASVTVHH1080 pKa = 5.94PPAYY1084 pKa = 10.08GFYY1087 pKa = 10.62QNGLLIFDD1095 pKa = 4.25YY1096 pKa = 10.92QSPTNFKK1103 pKa = 10.41FAGAYY1108 pKa = 10.1AGTDD1112 pKa = 2.94QWVIGHH1118 pKa = 6.3RR1119 pKa = 11.84TSQWITDD1126 pKa = 3.63ASAGASIDD1134 pKa = 3.36VGTDD1138 pKa = 3.16YY1139 pKa = 11.05EE1140 pKa = 4.43LSVVIEE1146 pKa = 4.48GDD1148 pKa = 3.56HH1149 pKa = 6.08QVTLLVDD1156 pKa = 4.07GVPQTSHH1163 pKa = 6.16TFSDD1167 pKa = 3.81SLTDD1171 pKa = 3.44GDD1173 pKa = 5.28LGFGTWNAVASYY1185 pKa = 11.09DD1186 pKa = 3.86DD1187 pKa = 4.49VSVQSYY1193 pKa = 7.67VAPPPPPPPPAGTLPVAEE1211 pKa = 5.05DD1212 pKa = 4.1FSDD1215 pKa = 4.19GVADD1219 pKa = 3.65YY1220 pKa = 7.93FQPQVGDD1227 pKa = 3.29WAINAGRR1234 pKa = 11.84YY1235 pKa = 9.14VGTPAGDD1242 pKa = 3.56AVSTLALDD1250 pKa = 4.08APLPSDD1256 pKa = 4.94LEE1258 pKa = 4.16FQATVNIQPPSGGLYY1273 pKa = 10.35QNGLLVFDD1281 pKa = 4.51YY1282 pKa = 10.88QSPTDD1287 pKa = 3.85FKK1289 pKa = 11.29FAGAYY1294 pKa = 10.12AGTNQWVIGHH1304 pKa = 6.21RR1305 pKa = 11.84TSQWVTDD1312 pKa = 4.03AFAGSTVNVGVDD1324 pKa = 3.15YY1325 pKa = 11.0DD1326 pKa = 3.81LRR1328 pKa = 11.84LVIEE1332 pKa = 5.02GGNQVTLWVGEE1343 pKa = 4.53TPQVTYY1349 pKa = 11.15SFAEE1353 pKa = 4.18SVTDD1357 pKa = 3.82GDD1359 pKa = 4.3IGLGTWNAVAKK1370 pKa = 10.01YY1371 pKa = 10.08DD1372 pKa = 3.9QIVVQAYY1379 pKa = 6.74TPPPPPPAAEE1389 pKa = 4.35LPVQEE1394 pKa = 5.45DD1395 pKa = 4.25YY1396 pKa = 11.82SDD1398 pKa = 3.73GLADD1402 pKa = 3.95LFVPRR1407 pKa = 11.84RR1408 pKa = 11.84GTWTVSGGEE1417 pKa = 4.11YY1418 pKa = 9.79HH1419 pKa = 7.53ASPSGDD1425 pKa = 3.54AISTLEE1431 pKa = 4.34PGGALPTDD1439 pKa = 4.31LEE1441 pKa = 4.47FQATARR1447 pKa = 11.84IQPPTYY1453 pKa = 10.22GVYY1456 pKa = 10.44QNAVVIFDD1464 pKa = 3.92YY1465 pKa = 11.01QSPTNFKK1472 pKa = 10.41FAGAYY1477 pKa = 10.1AGTNEE1482 pKa = 4.07WVIGTRR1488 pKa = 11.84TSSWTVLASYY1498 pKa = 11.3GEE1500 pKa = 4.46TVNVNVDD1507 pKa = 3.14YY1508 pKa = 10.39TLRR1511 pKa = 11.84LVIDD1515 pKa = 4.53GDD1517 pKa = 3.87HH1518 pKa = 5.99QATLFVDD1525 pKa = 4.54GLPKK1529 pKa = 8.5TTHH1532 pKa = 5.91TFNGSLTDD1540 pKa = 3.87GSVGLATWNAIAHH1553 pKa = 6.37FDD1555 pKa = 4.23DD1556 pKa = 4.91VSLQATGTSVAALTVGTSVLPEE1578 pKa = 4.06TNADD1582 pKa = 3.55LSDD1585 pKa = 4.43PLLSLPAASSRR1596 pKa = 11.84RR1597 pKa = 11.84SSAGIGASPSDD1608 pKa = 3.75TSAGAASHH1616 pKa = 6.2GRR1618 pKa = 11.84GNGRR1622 pKa = 11.84PGSPQSISSEE1632 pKa = 4.25SPLDD1636 pKa = 3.47QVFAEE1641 pKa = 4.79LEE1643 pKa = 3.89FDD1645 pKa = 5.01LMGDD1649 pKa = 4.12LSS1651 pKa = 3.72

Molecular weight: 173.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M6DFK1|A0A5M6DFK1_9BACT Uncharacterized protein OS=Roseiconus nitratireducens OX=2605748 GN=FYK55_04130 PE=4 SV=1
MM1 pKa = 7.23SRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84SPLAAHH11 pKa = 6.89HH12 pKa = 6.6SPLTTRR18 pKa = 11.84HH19 pKa = 5.83SPLTTHH25 pKa = 7.14HH26 pKa = 6.77SPLATRR32 pKa = 11.84HH33 pKa = 5.75SPLTTHH39 pKa = 7.14HH40 pKa = 6.77SPLATRR46 pKa = 11.84HH47 pKa = 5.78SPLATRR53 pKa = 11.84HH54 pKa = 5.75SPLTTHH60 pKa = 7.14HH61 pKa = 6.76SPLTTHH67 pKa = 7.06HH68 pKa = 6.76SPLTTHH74 pKa = 7.12HH75 pKa = 6.8SPLTTRR81 pKa = 11.84YY82 pKa = 10.15SLLATHH88 pKa = 7.43RR89 pKa = 3.95

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5599

0

5599

2301654

19

11909

411.1

45.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.857 ± 0.037

1.101 ± 0.015

6.649 ± 0.041

5.966 ± 0.027

3.598 ± 0.02

7.911 ± 0.045

2.178 ± 0.02

4.761 ± 0.021

2.987 ± 0.03

9.688 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.019

2.916 ± 0.033

5.52 ± 0.028

4.298 ± 0.023

7.274 ± 0.048

6.632 ± 0.03

5.468 ± 0.037

7.262 ± 0.027

1.533 ± 0.018

2.291 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski