Sphingobium sp. AP49

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J2WCN9|J2WCN9_9SPHN Membrane protein involved in the export of O-antigen and teichoic acid OS=Sphingobium sp. AP49 OX=1144307 GN=PMI04_03548 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TTYY5 pKa = 11.22DD6 pKa = 3.12SATVRR11 pKa = 11.84LYY13 pKa = 11.04HH14 pKa = 7.0LGDD17 pKa = 5.03DD18 pKa = 4.26GAAMTIMYY26 pKa = 9.73GPLSEE31 pKa = 4.35ALRR34 pKa = 11.84VAEE37 pKa = 4.02QQPQEE42 pKa = 4.22VQDD45 pKa = 4.07GLFLATDD52 pKa = 3.84NDD54 pKa = 3.68VVAYY58 pKa = 10.51LDD60 pKa = 4.88LIEE63 pKa = 4.28SS64 pKa = 3.94

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J2D7N9|J2D7N9_9SPHN Putative membrane protein OS=Sphingobium sp. AP49 OX=1144307 GN=PMI04_00679 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NIMRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4212

0

4212

1352865

21

2962

321.2

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.283 ± 0.066

0.764 ± 0.01

6.219 ± 0.03

4.899 ± 0.033

3.475 ± 0.021

8.931 ± 0.037

2.055 ± 0.019

5.264 ± 0.025

3.024 ± 0.026

9.942 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.018

2.583 ± 0.026

5.421 ± 0.029

3.37 ± 0.02

7.046 ± 0.046

5.232 ± 0.036

5.213 ± 0.036

6.848 ± 0.03

1.484 ± 0.017

2.362 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski