Amino acid dipepetide frequency for Cyanobacterium aponinum (strain PCC 10605)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.024AlaAla: 3.024 ± 0.076	0.62AlaCys: 0.62 ± 0.027	3.187AlaAsp: 3.187 ± 0.057	4.289AlaGlu: 4.289 ± 0.073	2.526AlaPhe: 2.526 ± 0.055	4.164AlaGly: 4.164 ± 0.077	1.054AlaHis: 1.054 ± 0.029	6.261AlaIle: 6.261 ± 0.076	4.024AlaLys: 4.024 ± 0.071	6.84AlaLeu: 6.84 ± 0.084	1.41AlaMet: 1.41 ± 0.038	3.254AlaAsn: 3.254 ± 0.073	1.951AlaPro: 1.951 ± 0.05	3.376AlaGln: 3.376 ± 0.063	2.627AlaArg: 2.627 ± 0.056	4.044AlaSer: 4.044 ± 0.067	3.491AlaThr: 3.491 ± 0.073	4.007AlaVal: 4.007 ± 0.081	0.718AlaTrp: 0.718 ± 0.022	2.04AlaTyr: 2.04 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.549CysAla: 0.549 ± 0.026	0.157CysCys: 0.157 ± 0.013	0.684CysAsp: 0.684 ± 0.028	0.535CysGlu: 0.535 ± 0.024	0.504CysPhe: 0.504 ± 0.022	0.764CysGly: 0.764 ± 0.029	0.298CysHis: 0.298 ± 0.019	0.575CysIle: 0.575 ± 0.024	0.372CysLys: 0.372 ± 0.018	1.291CysLeu: 1.291 ± 0.035	0.127CysMet: 0.127 ± 0.011	0.397CysAsn: 0.397 ± 0.018	0.523CysPro: 0.523 ± 0.025	0.66CysGln: 0.66 ± 0.027	0.464CysArg: 0.464 ± 0.02	0.569CysSer: 0.569 ± 0.022	0.42CysThr: 0.42 ± 0.021	0.563CysVal: 0.563 ± 0.024	0.141CysTrp: 0.141 ± 0.014	0.419CysTyr: 0.419 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	2.597AspAla: 2.597 ± 0.047	0.515AspCys: 0.515 ± 0.023	2.578AspAsp: 2.578 ± 0.07	3.185AspGlu: 3.185 ± 0.064	2.761AspPhe: 2.761 ± 0.055	3.09AspGly: 3.09 ± 0.086	0.716AspHis: 0.716 ± 0.028	4.303AspIle: 4.303 ± 0.062	3.059AspLys: 3.059 ± 0.061	6.308AspLeu: 6.308 ± 0.07	0.762AspMet: 0.762 ± 0.027	3.354AspAsn: 3.354 ± 0.068	1.794AspPro: 1.794 ± 0.05	1.599AspGln: 1.599 ± 0.038	2.437AspArg: 2.437 ± 0.047	3.279AspSer: 3.279 ± 0.061	2.495AspThr: 2.495 ± 0.052	2.902AspVal: 2.902 ± 0.059	0.781AspTrp: 0.781 ± 0.03	2.268AspTyr: 2.268 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.339GluAla: 4.339 ± 0.069	0.522GluCys: 0.522 ± 0.023	3.473GluAsp: 3.473 ± 0.068	5.462GluGlu: 5.462 ± 0.106	2.494GluPhe: 2.494 ± 0.053	3.848GluGly: 3.848 ± 0.079	0.726GluHis: 0.726 ± 0.031	6.37GluIle: 6.37 ± 0.086	6.221GluLys: 6.221 ± 0.118	6.857GluLeu: 6.857 ± 0.095	1.41GluMet: 1.41 ± 0.039	4.775GluAsn: 4.775 ± 0.08	1.635GluPro: 1.635 ± 0.043	2.867GluGln: 2.867 ± 0.059	2.559GluArg: 2.559 ± 0.062	3.715GluSer: 3.715 ± 0.06	3.819GluThr: 3.819 ± 0.063	3.98GluVal: 3.98 ± 0.071	0.753GluTrp: 0.753 ± 0.03	2.232GluTyr: 2.232 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	2.953PheAla: 2.953 ± 0.062	0.613PheCys: 0.613 ± 0.023	2.37PheAsp: 2.37 ± 0.053	2.219PheGlu: 2.219 ± 0.05	2.153PhePhe: 2.153 ± 0.051	2.834PheGly: 2.834 ± 0.059	0.734PheHis: 0.734 ± 0.028	3.428PheIle: 3.428 ± 0.061	1.971PheLys: 1.971 ± 0.045	4.655PheLeu: 4.655 ± 0.083	0.743PheMet: 0.743 ± 0.026	2.519PheAsn: 2.519 ± 0.065	1.909PhePro: 1.909 ± 0.042	1.775PheGln: 1.775 ± 0.039	1.656PheArg: 1.656 ± 0.041	3.409PheSer: 3.409 ± 0.058	2.512PheThr: 2.512 ± 0.063	2.44PheVal: 2.44 ± 0.044	0.708PheTrp: 0.708 ± 0.027	1.666PheTyr: 1.666 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	4.004GlyAla: 4.004 ± 0.086	0.761GlyCys: 0.761 ± 0.031	3.471GlyAsp: 3.471 ± 0.072	4.423GlyGlu: 4.423 ± 0.081	3.089GlyPhe: 3.089 ± 0.064	4.584GlyGly: 4.584 ± 0.129	0.987GlyHis: 0.987 ± 0.034	5.372GlyIle: 5.372 ± 0.079	4.717GlyLys: 4.717 ± 0.079	6.269GlyLeu: 6.269 ± 0.075	1.333GlyMet: 1.333 ± 0.041	3.425GlyAsn: 3.425 ± 0.124	0.854GlyPro: 0.854 ± 0.032	2.173GlyGln: 2.173 ± 0.056	2.387GlyArg: 2.387 ± 0.062	3.58GlySer: 3.58 ± 0.085	3.117GlyThr: 3.117 ± 0.087	4.468GlyVal: 4.468 ± 0.07	0.924GlyTrp: 0.924 ± 0.035	2.458GlyTyr: 2.458 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	0.725HisAla: 0.725 ± 0.025	0.262HisCys: 0.262 ± 0.013	0.791HisAsp: 0.791 ± 0.029	0.858HisGlu: 0.858 ± 0.031	0.838HisPhe: 0.838 ± 0.028	1.021HisGly: 1.021 ± 0.026	0.641HisHis: 0.641 ± 0.025	1.153HisIle: 1.153 ± 0.032	0.909HisLys: 0.909 ± 0.027	2.102HisLeu: 2.102 ± 0.046	0.164HisMet: 0.164 ± 0.014	0.91HisAsn: 0.91 ± 0.033	1.173HisPro: 1.173 ± 0.03	1.107HisGln: 1.107 ± 0.034	0.865HisArg: 0.865 ± 0.03	1.134HisSer: 1.134 ± 0.031	0.744HisThr: 0.744 ± 0.025	0.681HisVal: 0.681 ± 0.025	0.318HisTrp: 0.318 ± 0.019	0.689HisTyr: 0.689 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.766IleAla: 6.766 ± 0.081	0.897IleCys: 0.897 ± 0.029	4.522IleAsp: 4.522 ± 0.059	5.429IleGlu: 5.429 ± 0.069	3.604IlePhe: 3.604 ± 0.061	4.803IleGly: 4.803 ± 0.083	1.351IleHis: 1.351 ± 0.04	6.973IleIle: 6.973 ± 0.108	5.134IleLys: 5.134 ± 0.076	8.365IleLeu: 8.365 ± 0.106	1.194IleMet: 1.194 ± 0.035	5.387IleAsn: 5.387 ± 0.102	4.159IlePro: 4.159 ± 0.059	2.94IleGln: 2.94 ± 0.05	3.173IleArg: 3.173 ± 0.052	5.686IleSer: 5.686 ± 0.086	4.86IleThr: 4.86 ± 0.081	4.722IleVal: 4.722 ± 0.065	0.927IleTrp: 0.927 ± 0.033	2.739IleTyr: 2.739 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	3.94LysAla: 3.94 ± 0.069	0.465LysCys: 0.465 ± 0.025	2.908LysAsp: 2.908 ± 0.062	4.741LysGlu: 4.741 ± 0.093	2.074LysPhe: 2.074 ± 0.044	3.423LysGly: 3.423 ± 0.062	0.837LysHis: 0.837 ± 0.033	5.946LysIle: 5.946 ± 0.104	4.365LysLys: 4.365 ± 0.092	6.311LysLeu: 6.311 ± 0.096	1.306LysMet: 1.306 ± 0.035	3.962LysAsn: 3.962 ± 0.075	2.487LysPro: 2.487 ± 0.056	3.258LysGln: 3.258 ± 0.071	2.333LysArg: 2.333 ± 0.055	3.734LysSer: 3.734 ± 0.07	3.562LysThr: 3.562 ± 0.056	3.628LysVal: 3.628 ± 0.063	0.706LysTrp: 0.706 ± 0.025	2.219LysTyr: 2.219 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	7.614LeuAla: 7.614 ± 0.084	0.952LeuCys: 0.952 ± 0.029	5.5LeuAsp: 5.5 ± 0.079	7.886LeuGlu: 7.886 ± 0.108	4.18LeuPhe: 4.18 ± 0.075	7.014LeuGly: 7.014 ± 0.093	1.64LeuHis: 1.64 ± 0.043	8.686LeuIle: 8.686 ± 0.113	6.894LeuLys: 6.894 ± 0.109	10.384LeuLeu: 10.384 ± 0.123	2.064LeuMet: 2.064 ± 0.049	5.995LeuAsn: 5.995 ± 0.098	4.984LeuPro: 4.984 ± 0.066	4.863LeuGln: 4.863 ± 0.086	4.343LeuArg: 4.343 ± 0.068	7.667LeuSer: 7.667 ± 0.089	6.56LeuThr: 6.56 ± 0.08	6.381LeuVal: 6.381 ± 0.072	1.303LeuTrp: 1.303 ± 0.041	2.918LeuTyr: 2.918 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	1.55MetAla: 1.55 ± 0.036	0.132MetCys: 0.132 ± 0.009	0.818MetAsp: 0.818 ± 0.029	1.112MetGlu: 1.112 ± 0.034	0.594MetPhe: 0.594 ± 0.023	1.483MetGly: 1.483 ± 0.042	0.178MetHis: 0.178 ± 0.014	1.459MetIle: 1.459 ± 0.037	1.194MetLys: 1.194 ± 0.035	1.669MetLeu: 1.669 ± 0.042	0.475MetMet: 0.475 ± 0.022	1.009MetAsn: 1.009 ± 0.028	0.759MetPro: 0.759 ± 0.026	0.598MetGln: 0.598 ± 0.023	0.787MetArg: 0.787 ± 0.029	1.336MetSer: 1.336 ± 0.036	1.307MetThr: 1.307 ± 0.036	1.239MetVal: 1.239 ± 0.038	0.169MetTrp: 0.169 ± 0.013	0.359MetTyr: 0.359 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.827AsnAla: 2.827 ± 0.057	0.601AsnCys: 0.601 ± 0.027	2.419AsnAsp: 2.419 ± 0.074	2.56AsnGlu: 2.56 ± 0.052	2.911AsnPhe: 2.911 ± 0.053	2.971AsnGly: 2.971 ± 0.111	1.366AsnHis: 1.366 ± 0.036	5.007AsnIle: 5.007 ± 0.11	3.117AsnLys: 3.117 ± 0.064	7.415AsnLeu: 7.415 ± 0.123	0.998AsnMet: 0.998 ± 0.03	4.353AsnAsn: 4.353 ± 0.118	3.406AsnPro: 3.406 ± 0.071	3.741AsnGln: 3.741 ± 0.07	2.396AsnArg: 2.396 ± 0.05	4.194AsnSer: 4.194 ± 0.102	2.784AsnThr: 2.784 ± 0.079	2.563AsnVal: 2.563 ± 0.059	0.839AsnTrp: 0.839 ± 0.027	2.451AsnTyr: 2.451 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	2.027ProAla: 2.027 ± 0.045	0.354ProCys: 0.354 ± 0.021	2.496ProAsp: 2.496 ± 0.048	3.598ProGlu: 3.598 ± 0.059	1.863ProPhe: 1.863 ± 0.045	1.671ProGly: 1.671 ± 0.05	0.931ProHis: 0.931 ± 0.031	3.158ProIle: 3.158 ± 0.054	1.933ProLys: 1.933 ± 0.048	4.55ProLeu: 4.55 ± 0.076	0.623ProMet: 0.623 ± 0.023	2.272ProAsn: 2.272 ± 0.052	1.892ProPro: 1.892 ± 0.055	2.301ProGln: 2.301 ± 0.05	1.437ProArg: 1.437 ± 0.034	2.888ProSer: 2.888 ± 0.057	2.202ProThr: 2.202 ± 0.049	2.587ProVal: 2.587 ± 0.058	0.482ProTrp: 0.482 ± 0.021	1.397ProTyr: 1.397 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.047GlnAla: 3.047 ± 0.053	0.332GlnCys: 0.332 ± 0.018	2.271GlnAsp: 2.271 ± 0.049	4.317GlnGlu: 4.317 ± 0.08	1.644GlnPhe: 1.644 ± 0.043	3.213GlnGly: 3.213 ± 0.053	0.629GlnHis: 0.629 ± 0.024	4.009GlnIle: 4.009 ± 0.075	3.565GlnLys: 3.565 ± 0.068	4.997GlnLeu: 4.997 ± 0.084	0.865GlnMet: 0.865 ± 0.029	2.621GlnAsn: 2.621 ± 0.054	1.944GlnPro: 1.944 ± 0.047	2.67GlnGln: 2.67 ± 0.066	1.898GlnArg: 1.898 ± 0.041	2.676GlnSer: 2.676 ± 0.058	2.353GlnThr: 2.353 ± 0.05	2.868GlnVal: 2.868 ± 0.049	0.776GlnTrp: 0.776 ± 0.028	1.378GlnTyr: 1.378 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.177ArgAla: 2.177 ± 0.048	0.429ArgCys: 0.429 ± 0.022	2.142ArgAsp: 2.142 ± 0.045	3.057ArgGlu: 3.057 ± 0.058	1.929ArgPhe: 1.929 ± 0.043	2.401ArgGly: 2.401 ± 0.049	0.792ArgHis: 0.792 ± 0.028	3.232ArgIle: 3.232 ± 0.061	2.538ArgLys: 2.538 ± 0.055	4.627ArgLeu: 4.627 ± 0.071	0.751ArgMet: 0.751 ± 0.029	2.161ArgAsn: 2.161 ± 0.048	1.344ArgPro: 1.344 ± 0.037	2.476ArgGln: 2.476 ± 0.055	2.076ArgArg: 2.076 ± 0.06	2.184ArgSer: 2.184 ± 0.05	1.874ArgThr: 1.874 ± 0.045	2.541ArgVal: 2.541 ± 0.046	0.578ArgTrp: 0.578 ± 0.026	1.553ArgTyr: 1.553 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	4.27SerAla: 4.27 ± 0.068	0.683SerCys: 0.683 ± 0.026	3.043SerAsp: 3.043 ± 0.053	4.068SerGlu: 4.068 ± 0.073	2.986SerPhe: 2.986 ± 0.057	4.105SerGly: 4.105 ± 0.083	1.349SerHis: 1.349 ± 0.038	4.805SerIle: 4.805 ± 0.077	3.219SerLys: 3.219 ± 0.06	7.914SerLeu: 7.914 ± 0.096	1.186SerMet: 1.186 ± 0.036	3.603SerAsn: 3.603 ± 0.076	3.053SerPro: 3.053 ± 0.062	3.653SerGln: 3.653 ± 0.065	2.697SerArg: 2.697 ± 0.047	4.677SerSer: 4.677 ± 0.084	2.98SerThr: 2.98 ± 0.062	3.702SerVal: 3.702 ± 0.065	0.873SerTrp: 0.873 ± 0.03	2.108SerTyr: 2.108 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	3.662ThrAla: 3.662 ± 0.07	0.461ThrCys: 0.461 ± 0.021	2.599ThrAsp: 2.599 ± 0.058	3.533ThrGlu: 3.533 ± 0.064	2.207ThrPhe: 2.207 ± 0.055	3.869ThrGly: 3.869 ± 0.081	0.933ThrHis: 0.933 ± 0.026	4.85ThrIle: 4.85 ± 0.086	2.797ThrLys: 2.797 ± 0.045	5.778ThrLeu: 5.778 ± 0.087	0.874ThrMet: 0.874 ± 0.03	2.879ThrAsn: 2.879 ± 0.082	2.992ThrPro: 2.992 ± 0.066	2.336ThrGln: 2.336 ± 0.054	1.932ThrArg: 1.932 ± 0.043	3.177ThrSer: 3.177 ± 0.062	3.069ThrThr: 3.069 ± 0.076	3.624ThrVal: 3.624 ± 0.069	0.596ThrTrp: 0.596 ± 0.026	1.672ThrTyr: 1.672 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	4.38ValAla: 4.38 ± 0.068	0.671ValCys: 0.671 ± 0.026	3.12ValAsp: 3.12 ± 0.053	3.944ValGlu: 3.944 ± 0.06	2.558ValPhe: 2.558 ± 0.06	4.026ValGly: 4.026 ± 0.062	0.773ValHis: 0.773 ± 0.027	4.991ValIle: 4.991 ± 0.076	4.05ValLys: 4.05 ± 0.053	5.367ValLeu: 5.367 ± 0.067	1.242ValMet: 1.242 ± 0.032	3.779ValAsn: 3.779 ± 0.079	1.89ValPro: 1.89 ± 0.044	1.833ValGln: 1.833 ± 0.045	2.309ValArg: 2.309 ± 0.057	4.016ValSer: 4.016 ± 0.071	3.668ValThr: 3.668 ± 0.068	3.827ValVal: 3.827 ± 0.078	0.674ValTrp: 0.674 ± 0.026	1.863ValTyr: 1.863 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.694TrpAla: 0.694 ± 0.026	0.16TrpCys: 0.16 ± 0.013	0.607TrpAsp: 0.607 ± 0.028	0.919TrpGlu: 0.919 ± 0.035	0.634TrpPhe: 0.634 ± 0.025	0.954TrpGly: 0.954 ± 0.032	0.307TrpHis: 0.307 ± 0.017	0.831TrpIle: 0.831 ± 0.029	0.589TrpLys: 0.589 ± 0.025	1.806TrpLeu: 1.806 ± 0.05	0.276TrpMet: 0.276 ± 0.016	0.59TrpAsn: 0.59 ± 0.024	0.141TrpPro: 0.141 ± 0.011	1.315TrpGln: 1.315 ± 0.042	0.646TrpArg: 0.646 ± 0.029	0.73TrpSer: 0.73 ± 0.026	0.54TrpThr: 0.54 ± 0.023	0.663TrpVal: 0.663 ± 0.026	0.224TrpTrp: 0.224 ± 0.016	0.417TrpTyr: 0.417 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.764TyrAla: 1.764 ± 0.043	0.429TyrCys: 0.429 ± 0.022	1.782TyrAsp: 1.782 ± 0.043	1.921TyrGlu: 1.921 ± 0.045	1.718TyrPhe: 1.718 ± 0.045	2.158TyrGly: 2.158 ± 0.045	0.842TyrHis: 0.842 ± 0.028	2.222TyrIle: 2.222 ± 0.05	1.538TyrLys: 1.538 ± 0.043	4.321TyrLeu: 4.321 ± 0.068	0.424TyrMet: 0.424 ± 0.022	1.675TyrAsn: 1.675 ± 0.04	1.771TyrPro: 1.771 ± 0.033	2.533TyrGln: 2.533 ± 0.062	1.836TyrArg: 1.836 ± 0.041	2.284TyrSer: 2.284 ± 0.05	1.484TyrThr: 1.484 ± 0.043	1.594TyrVal: 1.594 ± 0.043	0.552TyrTrp: 0.552 ± 0.023	1.399TyrTyr: 1.399 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3415 proteins (1109571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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