Pseudoruegeria haliotis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Pseudoruegeria

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0RHZ5|A0A2T0RHZ5_9RHOB Phosphate acetyltransferase OS=Pseudoruegeria haliotis OX=1280846 GN=CLV78_11293 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84FLGVWAAFWLALITPASSADD22 pKa = 3.94DD23 pKa = 3.77SDD25 pKa = 5.28LPAPLQEE32 pKa = 4.4KK33 pKa = 10.4VEE35 pKa = 4.31AAQQACAEE43 pKa = 4.15FDD45 pKa = 3.05NGAFYY50 pKa = 11.05LEE52 pKa = 4.29WEE54 pKa = 4.19AVDD57 pKa = 5.71RR58 pKa = 11.84VDD60 pKa = 6.19LDD62 pKa = 3.65GDD64 pKa = 4.81LYY66 pKa = 10.43PDD68 pKa = 3.02WVLNEE73 pKa = 4.54RR74 pKa = 11.84GFSCSTAASLYY85 pKa = 10.11CGTGGCMSHH94 pKa = 7.22FLVEE98 pKa = 4.66DD99 pKa = 3.73VLSSLLNQGWGLADD113 pKa = 4.56LGPNKK118 pKa = 9.95VLLADD123 pKa = 3.57VHH125 pKa = 6.7GSQCGGINPTPCMTASTWDD144 pKa = 3.38SDD146 pKa = 3.58EE147 pKa = 4.3KK148 pKa = 10.63QWRR151 pKa = 11.84TTAAEE156 pKa = 4.04WEE158 pKa = 4.28

Molecular weight: 17.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0RIX9|A0A2T0RIX9_9RHOB DNA-directed RNA polymerase subunit beta' OS=Pseudoruegeria haliotis OX=1280846 GN=rpoC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4715

0

4715

1462637

27

5796

310.2

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.996 ± 0.05

0.914 ± 0.013

6.143 ± 0.037

6.126 ± 0.035

3.777 ± 0.022

8.846 ± 0.04

2.053 ± 0.018

5.084 ± 0.026

2.968 ± 0.027

9.942 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.018

2.583 ± 0.018

5.18 ± 0.024

2.983 ± 0.018

6.828 ± 0.044

5.356 ± 0.022

5.491 ± 0.031

7.452 ± 0.03

1.413 ± 0.015

2.156 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski