Amino acid dipepetide frequency for Bacillus obstructivus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.71AlaAla: 4.71 ± 0.079	0.555AlaCys: 0.555 ± 0.021	2.855AlaAsp: 2.855 ± 0.045	3.961AlaGlu: 3.961 ± 0.068	2.978AlaPhe: 2.978 ± 0.051	4.656AlaGly: 4.656 ± 0.075	1.105AlaHis: 1.105 ± 0.029	6.083AlaIle: 6.083 ± 0.087	4.578AlaLys: 4.578 ± 0.066	6.51AlaLeu: 6.51 ± 0.072	1.808AlaMet: 1.808 ± 0.039	2.766AlaAsn: 2.766 ± 0.048	1.818AlaPro: 1.818 ± 0.041	1.928AlaGln: 1.928 ± 0.042	2.329AlaArg: 2.329 ± 0.042	3.667AlaSer: 3.667 ± 0.055	3.288AlaThr: 3.288 ± 0.061	4.507AlaVal: 4.507 ± 0.063	0.582AlaTrp: 0.582 ± 0.023	2.134AlaTyr: 2.134 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.414CysAla: 0.414 ± 0.02	0.095CysCys: 0.095 ± 0.009	0.368CysAsp: 0.368 ± 0.017	0.483CysGlu: 0.483 ± 0.018	0.35CysPhe: 0.35 ± 0.016	0.649CysGly: 0.649 ± 0.024	0.193CysHis: 0.193 ± 0.011	0.651CysIle: 0.651 ± 0.024	0.419CysLys: 0.419 ± 0.019	0.745CysLeu: 0.745 ± 0.023	0.182CysMet: 0.182 ± 0.012	0.292CysAsn: 0.292 ± 0.017	0.343CysPro: 0.343 ± 0.016	0.267CysGln: 0.267 ± 0.013	0.273CysArg: 0.273 ± 0.014	0.546CysSer: 0.546 ± 0.02	0.391CysThr: 0.391 ± 0.017	0.427CysVal: 0.427 ± 0.019	0.071CysTrp: 0.071 ± 0.007	0.281CysTyr: 0.281 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.838AspAla: 2.838 ± 0.058	0.384AspCys: 0.384 ± 0.018	2.245AspAsp: 2.245 ± 0.049	4.101AspGlu: 4.101 ± 0.054	2.392AspPhe: 2.392 ± 0.043	3.128AspGly: 3.128 ± 0.057	1.285AspHis: 1.285 ± 0.035	4.409AspIle: 4.409 ± 0.058	3.176AspLys: 3.176 ± 0.053	4.866AspLeu: 4.866 ± 0.063	1.223AspMet: 1.223 ± 0.029	1.852AspAsn: 1.852 ± 0.037	1.945AspPro: 1.945 ± 0.041	2.242AspGln: 2.242 ± 0.038	2.127AspArg: 2.127 ± 0.032	2.612AspSer: 2.612 ± 0.048	2.254AspThr: 2.254 ± 0.037	3.369AspVal: 3.369 ± 0.051	0.641AspTrp: 0.641 ± 0.024	2.204AspTyr: 2.204 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.547GluAla: 4.547 ± 0.057	0.406GluCys: 0.406 ± 0.017	3.466GluAsp: 3.466 ± 0.057	6.232GluGlu: 6.232 ± 0.077	2.675GluPhe: 2.675 ± 0.047	3.939GluGly: 3.939 ± 0.055	1.44GluHis: 1.44 ± 0.037	6.18GluIle: 6.18 ± 0.081	7.002GluLys: 7.002 ± 0.08	6.799GluLeu: 6.799 ± 0.075	2.315GluMet: 2.315 ± 0.038	3.882GluAsn: 3.882 ± 0.054	1.828GluPro: 1.828 ± 0.038	3.049GluGln: 3.049 ± 0.057	3.178GluArg: 3.178 ± 0.057	3.498GluSer: 3.498 ± 0.049	3.557GluThr: 3.557 ± 0.05	4.59GluVal: 4.59 ± 0.066	0.821GluTrp: 0.821 ± 0.025	2.403GluTyr: 2.403 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.886PheAla: 2.886 ± 0.057	0.389PheCys: 0.389 ± 0.018	2.329PheAsp: 2.329 ± 0.041	2.831PheGlu: 2.831 ± 0.046	2.556PhePhe: 2.556 ± 0.05	3.306PheGly: 3.306 ± 0.053	1.137PheHis: 1.137 ± 0.033	4.609PheIle: 4.609 ± 0.071	2.684PheLys: 2.684 ± 0.042	4.824PheLeu: 4.824 ± 0.08	1.168PheMet: 1.168 ± 0.034	2.11PheAsn: 2.11 ± 0.043	1.707PhePro: 1.707 ± 0.038	1.719PheGln: 1.719 ± 0.034	1.512PheArg: 1.512 ± 0.035	3.454PheSer: 3.454 ± 0.054	2.614PheThr: 2.614 ± 0.043	3.028PheVal: 3.028 ± 0.05	0.509PheTrp: 0.509 ± 0.02	1.865PheTyr: 1.865 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.36GlyAla: 4.36 ± 0.072	0.623GlyCys: 0.623 ± 0.025	2.97GlyAsp: 2.97 ± 0.054	4.196GlyGlu: 4.196 ± 0.053	3.425GlyPhe: 3.425 ± 0.058	4.634GlyGly: 4.634 ± 0.073	1.329GlyHis: 1.329 ± 0.034	6.329GlyIle: 6.329 ± 0.072	5.28GlyLys: 5.28 ± 0.064	6.451GlyLeu: 6.451 ± 0.077	2.039GlyMet: 2.039 ± 0.037	2.953GlyAsn: 2.953 ± 0.055	1.72GlyPro: 1.72 ± 0.037	2.121GlyGln: 2.121 ± 0.04	2.479GlyArg: 2.479 ± 0.045	3.845GlySer: 3.845 ± 0.058	3.84GlyThr: 3.84 ± 0.058	4.703GlyVal: 4.703 ± 0.075	0.808GlyTrp: 0.808 ± 0.029	2.787GlyTyr: 2.787 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	1.261HisAla: 1.261 ± 0.028	0.203HisCys: 0.203 ± 0.013	1.02HisAsp: 1.02 ± 0.03	1.352HisGlu: 1.352 ± 0.037	1.144HisPhe: 1.144 ± 0.035	1.328HisGly: 1.328 ± 0.032	0.723HisHis: 0.723 ± 0.027	1.711HisIle: 1.711 ± 0.032	1.095HisLys: 1.095 ± 0.028	2.307HisLeu: 2.307 ± 0.042	0.5HisMet: 0.5 ± 0.018	0.789HisAsn: 0.789 ± 0.024	1.171HisPro: 1.171 ± 0.031	0.96HisGln: 0.96 ± 0.03	0.89HisArg: 0.89 ± 0.026	1.429HisSer: 1.429 ± 0.034	1.038HisThr: 1.038 ± 0.028	1.418HisVal: 1.418 ± 0.034	0.253HisTrp: 0.253 ± 0.014	0.906HisTyr: 0.906 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.191IleAla: 6.191 ± 0.068	0.776IleCys: 0.776 ± 0.025	4.749IleAsp: 4.749 ± 0.075	6.193IleGlu: 6.193 ± 0.07	3.917IlePhe: 3.917 ± 0.07	6.384IleGly: 6.384 ± 0.074	2.077IleHis: 2.077 ± 0.043	7.831IleIle: 7.831 ± 0.098	5.51IleLys: 5.51 ± 0.065	8.295IleLeu: 8.295 ± 0.089	2.057IleMet: 2.057 ± 0.037	4.07IleAsn: 4.07 ± 0.054	3.733IlePro: 3.733 ± 0.061	3.422IleGln: 3.422 ± 0.051	3.321IleArg: 3.321 ± 0.051	5.817IleSer: 5.817 ± 0.076	4.65IleThr: 4.65 ± 0.058	5.851IleVal: 5.851 ± 0.072	0.75IleTrp: 0.75 ± 0.029	2.832IleTyr: 2.832 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.374LysAla: 4.374 ± 0.063	0.359LysCys: 0.359 ± 0.016	4.145LysAsp: 4.145 ± 0.062	7.181LysGlu: 7.181 ± 0.074	2.186LysPhe: 2.186 ± 0.041	4.794LysGly: 4.794 ± 0.061	1.468LysHis: 1.468 ± 0.031	5.668LysIle: 5.668 ± 0.066	6.846LysLys: 6.846 ± 0.077	6.379LysLeu: 6.379 ± 0.078	2.415LysMet: 2.415 ± 0.039	4.063LysAsn: 4.063 ± 0.049	2.256LysPro: 2.256 ± 0.043	3.27LysGln: 3.27 ± 0.057	3.271LysArg: 3.271 ± 0.047	3.829LysSer: 3.829 ± 0.061	3.618LysThr: 3.618 ± 0.05	4.703LysVal: 4.703 ± 0.066	0.906LysTrp: 0.906 ± 0.029	2.593LysTyr: 2.593 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	6.571LeuAla: 6.571 ± 0.073	0.733LeuCys: 0.733 ± 0.026	4.691LeuAsp: 4.691 ± 0.062	6.321LeuGlu: 6.321 ± 0.079	5.169LeuPhe: 5.169 ± 0.085	6.399LeuGly: 6.399 ± 0.081	2.028LeuHis: 2.028 ± 0.044	8.201LeuIle: 8.201 ± 0.096	7.198LeuLys: 7.198 ± 0.073	10.32LeuLeu: 10.32 ± 0.122	2.576LeuMet: 2.576 ± 0.042	4.723LeuAsn: 4.723 ± 0.055	3.854LeuPro: 3.854 ± 0.048	3.599LeuGln: 3.599 ± 0.055	3.345LeuArg: 3.345 ± 0.046	6.931LeuSer: 6.931 ± 0.079	5.543LeuThr: 5.543 ± 0.067	6.045LeuVal: 6.045 ± 0.079	0.846LeuTrp: 0.846 ± 0.028	3.179LeuTyr: 3.179 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.79MetAla: 1.79 ± 0.035	0.141MetCys: 0.141 ± 0.01	1.451MetAsp: 1.451 ± 0.028	1.976MetGlu: 1.976 ± 0.035	1.069MetPhe: 1.069 ± 0.03	1.84MetGly: 1.84 ± 0.036	0.402MetHis: 0.402 ± 0.016	2.517MetIle: 2.517 ± 0.045	2.645MetLys: 2.645 ± 0.044	2.427MetLeu: 2.427 ± 0.041	0.875MetMet: 0.875 ± 0.03	1.738MetAsn: 1.738 ± 0.039	0.895MetPro: 0.895 ± 0.028	0.864MetGln: 0.864 ± 0.025	0.988MetArg: 0.988 ± 0.027	1.68MetSer: 1.68 ± 0.039	1.517MetThr: 1.517 ± 0.028	1.748MetVal: 1.748 ± 0.036	0.2MetTrp: 0.2 ± 0.012	0.763MetTyr: 0.763 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.645AsnAla: 2.645 ± 0.054	0.356AsnCys: 0.356 ± 0.018	2.576AsnAsp: 2.576 ± 0.057	3.898AsnGlu: 3.898 ± 0.054	1.829AsnPhe: 1.829 ± 0.038	3.668AsnGly: 3.668 ± 0.059	1.265AsnHis: 1.265 ± 0.033	4.088AsnIle: 4.088 ± 0.059	3.604AsnLys: 3.604 ± 0.056	4.075AsnLeu: 4.075 ± 0.062	1.201AsnMet: 1.201 ± 0.024	2.297AsnAsn: 2.297 ± 0.053	2.158AsnPro: 2.158 ± 0.037	2.358AsnGln: 2.358 ± 0.049	2.104AsnArg: 2.104 ± 0.042	2.532AsnSer: 2.532 ± 0.05	2.17AsnThr: 2.17 ± 0.044	2.957AsnVal: 2.957 ± 0.045	0.566AsnTrp: 0.566 ± 0.02	1.735AsnTyr: 1.735 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	1.88ProAla: 1.88 ± 0.04	0.216ProCys: 0.216 ± 0.013	1.809ProAsp: 1.809 ± 0.04	2.677ProGlu: 2.677 ± 0.053	2.096ProPhe: 2.096 ± 0.04	2.107ProGly: 2.107 ± 0.041	0.828ProHis: 0.828 ± 0.024	3.282ProIle: 3.282 ± 0.055	2.386ProLys: 2.386 ± 0.049	3.522ProLeu: 3.522 ± 0.057	0.843ProMet: 0.843 ± 0.028	1.963ProAsn: 1.963 ± 0.04	0.973ProPro: 0.973 ± 0.03	1.044ProGln: 1.044 ± 0.031	1.025ProArg: 1.025 ± 0.029	2.295ProSer: 2.295 ± 0.043	1.97ProThr: 1.97 ± 0.037	2.514ProVal: 2.514 ± 0.043	0.371ProTrp: 0.371 ± 0.018	1.419ProTyr: 1.419 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	2.435GlnAla: 2.435 ± 0.044	0.238GlnCys: 0.238 ± 0.014	1.625GlnAsp: 1.625 ± 0.037	2.747GlnGlu: 2.747 ± 0.049	1.786GlnPhe: 1.786 ± 0.037	2.099GlnGly: 2.099 ± 0.04	0.79GlnHis: 0.79 ± 0.025	2.935GlnIle: 2.935 ± 0.057	2.894GlnLys: 2.894 ± 0.044	4.282GlnLeu: 4.282 ± 0.059	1.171GlnMet: 1.171 ± 0.028	1.714GlnAsn: 1.714 ± 0.038	1.214GlnPro: 1.214 ± 0.031	1.746GlnGln: 1.746 ± 0.042	1.423GlnArg: 1.423 ± 0.035	2.329GlnSer: 2.329 ± 0.043	1.975GlnThr: 1.975 ± 0.038	2.308GlnVal: 2.308 ± 0.038	0.445GlnTrp: 0.445 ± 0.016	1.45GlnTyr: 1.45 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.199ArgAla: 2.199 ± 0.044	0.247ArgCys: 0.247 ± 0.014	1.85ArgAsp: 1.85 ± 0.039	2.878ArgGlu: 2.878 ± 0.05	1.857ArgPhe: 1.857 ± 0.036	2.212ArgGly: 2.212 ± 0.043	0.772ArgHis: 0.772 ± 0.024	3.187ArgIle: 3.187 ± 0.048	3.292ArgLys: 3.292 ± 0.048	3.88ArgLeu: 3.88 ± 0.055	1.19ArgMet: 1.19 ± 0.027	1.969ArgAsn: 1.969 ± 0.038	1.171ArgPro: 1.171 ± 0.031	1.4ArgGln: 1.4 ± 0.031	1.699ArgArg: 1.699 ± 0.039	2.121ArgSer: 2.121 ± 0.038	1.946ArgThr: 1.946 ± 0.032	2.372ArgVal: 2.372 ± 0.041	0.4ArgTrp: 0.4 ± 0.018	1.419ArgTyr: 1.419 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.43SerAla: 3.43 ± 0.056	0.423SerCys: 0.423 ± 0.02	2.831SerAsp: 2.831 ± 0.044	3.894SerGlu: 3.894 ± 0.06	3.461SerPhe: 3.461 ± 0.063	4.292SerGly: 4.292 ± 0.063	1.216SerHis: 1.216 ± 0.034	5.9SerIle: 5.9 ± 0.077	4.403SerLys: 4.403 ± 0.054	6.341SerLeu: 6.341 ± 0.074	1.704SerMet: 1.704 ± 0.034	2.812SerAsn: 2.812 ± 0.049	2.154SerPro: 2.154 ± 0.038	2.025SerGln: 2.025 ± 0.036	2.15SerArg: 2.15 ± 0.039	4.072SerSer: 4.072 ± 0.061	3.166SerThr: 3.166 ± 0.045	3.862SerVal: 3.862 ± 0.055	0.654SerTrp: 0.654 ± 0.022	2.336SerTyr: 2.336 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	3.476ThrAla: 3.476 ± 0.054	0.36ThrCys: 0.36 ± 0.016	2.472ThrAsp: 2.472 ± 0.048	3.241ThrGlu: 3.241 ± 0.043	2.666ThrPhe: 2.666 ± 0.039	3.93ThrGly: 3.93 ± 0.06	1.023ThrHis: 1.023 ± 0.027	5.125ThrIle: 5.125 ± 0.072	3.534ThrLys: 3.534 ± 0.05	5.05ThrLeu: 5.05 ± 0.06	1.271ThrMet: 1.271 ± 0.034	2.713ThrAsn: 2.713 ± 0.047	2.119ThrPro: 2.119 ± 0.041	1.339ThrGln: 1.339 ± 0.032	1.692ThrArg: 1.692 ± 0.035	3.266ThrSer: 3.266 ± 0.051	2.887ThrThr: 2.887 ± 0.057	3.768ThrVal: 3.768 ± 0.048	0.521ThrTrp: 0.521 ± 0.019	1.902ThrTyr: 1.902 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	4.314ValAla: 4.314 ± 0.062	0.533ValCys: 0.533 ± 0.021	3.378ValAsp: 3.378 ± 0.052	4.454ValGlu: 4.454 ± 0.065	3.128ValPhe: 3.128 ± 0.048	4.355ValGly: 4.355 ± 0.063	1.29ValHis: 1.29 ± 0.029	5.877ValIle: 5.877 ± 0.067	4.687ValLys: 4.687 ± 0.065	6.336ValLeu: 6.336 ± 0.071	1.726ValMet: 1.726 ± 0.034	3.22ValAsn: 3.22 ± 0.054	2.458ValPro: 2.458 ± 0.042	2.248ValGln: 2.248 ± 0.04	2.295ValArg: 2.295 ± 0.041	4.257ValSer: 4.257 ± 0.059	3.611ValThr: 3.611 ± 0.065	4.434ValVal: 4.434 ± 0.069	0.59ValTrp: 0.59 ± 0.019	2.106ValTyr: 2.106 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.574TrpAla: 0.574 ± 0.023	0.086TrpCys: 0.086 ± 0.007	0.554TrpAsp: 0.554 ± 0.022	0.657TrpGlu: 0.657 ± 0.023	0.545TrpPhe: 0.545 ± 0.02	0.706TrpGly: 0.706 ± 0.029	0.214TrpHis: 0.214 ± 0.015	0.958TrpIle: 0.958 ± 0.029	0.82TrpLys: 0.82 ± 0.026	1.151TrpLeu: 1.151 ± 0.033	0.349TrpMet: 0.349 ± 0.018	0.61TrpAsn: 0.61 ± 0.021	0.251TrpPro: 0.251 ± 0.015	0.31TrpGln: 0.31 ± 0.016	0.408TrpArg: 0.408 ± 0.017	0.639TrpSer: 0.639 ± 0.023	0.502TrpThr: 0.502 ± 0.02	0.642TrpVal: 0.642 ± 0.027	0.159TrpTrp: 0.159 ± 0.011	0.375TrpTyr: 0.375 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.923TyrAla: 1.923 ± 0.039	0.319TyrCys: 0.319 ± 0.017	1.909TyrAsp: 1.909 ± 0.039	2.533TyrGlu: 2.533 ± 0.048	2.008TyrPhe: 2.008 ± 0.041	2.464TyrGly: 2.464 ± 0.04	0.912TyrHis: 0.912 ± 0.027	2.886TyrIle: 2.886 ± 0.043	2.274TyrLys: 2.274 ± 0.04	3.712TyrLeu: 3.712 ± 0.055	0.911TyrMet: 0.911 ± 0.03	1.548TyrAsn: 1.548 ± 0.035	1.451TyrPro: 1.451 ± 0.034	1.696TyrGln: 1.696 ± 0.04	1.553TyrArg: 1.553 ± 0.034	2.306TyrSer: 2.306 ± 0.048	1.818TyrThr: 1.818 ± 0.038	2.051TyrVal: 2.051 ± 0.043	0.416TyrTrp: 0.416 ± 0.017	1.53TyrTyr: 1.53 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4972 proteins (1385854 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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