Dehalobacter sp. CF

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Dehalobacter; unclassified Dehalobacter

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4L1G4|K4L1G4_9FIRM Non-specific protein-tyrosine kinase OS=Dehalobacter sp. CF OX=1131462 GN=DCF50_p1122 PE=3 SV=1
MM1 pKa = 8.44DD2 pKa = 3.86YY3 pKa = 9.99PYY5 pKa = 10.81LIGYY9 pKa = 7.99VYY11 pKa = 10.54SEE13 pKa = 4.48EE14 pKa = 4.14GRR16 pKa = 11.84SEE18 pKa = 4.37HH19 pKa = 6.18FLKK22 pKa = 10.15ATPTNIASFIVKK34 pKa = 10.32NSPLDD39 pKa = 3.7VIQITTPLDD48 pKa = 3.26TAFISTRR55 pKa = 11.84AGFIDD60 pKa = 3.8YY61 pKa = 10.83CADD64 pKa = 3.4QEE66 pKa = 4.28FLRR69 pKa = 11.84NEE71 pKa = 4.32LLPVLIPMQMGDD83 pKa = 3.68TEE85 pKa = 4.3PPEE88 pKa = 4.15VEE90 pKa = 4.07LVPEE94 pKa = 4.09NEE96 pKa = 4.21MNSMDD101 pKa = 4.65EE102 pKa = 4.3EE103 pKa = 4.96GLDD106 pKa = 3.52SLEE109 pKa = 4.13FF110 pKa = 4.75

Molecular weight: 12.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4KYA0|K4KYA0_9FIRM DEAD/DEAH box helicase-like protein OS=Dehalobacter sp. CF OX=1131462 GN=DCF50_p46 PE=4 SV=1
MM1 pKa = 7.52ANAAVKK7 pKa = 10.19KK8 pKa = 10.0EE9 pKa = 3.8RR10 pKa = 11.84GRR12 pKa = 11.84RR13 pKa = 11.84PRR15 pKa = 11.84KK16 pKa = 9.14RR17 pKa = 11.84VCSFCVDD24 pKa = 3.17KK25 pKa = 11.63VEE27 pKa = 5.07SIDD30 pKa = 4.14YY31 pKa = 10.87KK32 pKa = 10.62DD33 pKa = 3.45TQKK36 pKa = 10.61LRR38 pKa = 11.84KK39 pKa = 9.71YY40 pKa = 7.89ITEE43 pKa = 4.01RR44 pKa = 11.84GKK46 pKa = 10.04ILPRR50 pKa = 11.84RR51 pKa = 11.84ISGNCAKK58 pKa = 10.17HH59 pKa = 4.76QRR61 pKa = 11.84QVTIAVKK68 pKa = 10.01RR69 pKa = 11.84ARR71 pKa = 11.84SIALLPYY78 pKa = 9.92MIDD81 pKa = 3.12

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2936

0

2936

860573

37

2881

293.1

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.64 ± 0.043

1.191 ± 0.018

5.208 ± 0.031

7.072 ± 0.054

4.068 ± 0.036

7.063 ± 0.043

1.713 ± 0.019

7.817 ± 0.04

6.58 ± 0.044

10.013 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.025

4.392 ± 0.031

3.735 ± 0.029

3.693 ± 0.03

4.631 ± 0.039

6.055 ± 0.041

5.272 ± 0.037

6.723 ± 0.038

0.935 ± 0.015

3.523 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski