Proteus phage 10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 232 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7J7E3|A0A7D7J7E3_9CAUD Uncharacterized protein OS=Proteus phage 10 OX=2759182 PE=4 SV=1
MM1 pKa = 7.85VIIDD5 pKa = 3.9EE6 pKa = 4.68SKK8 pKa = 9.78TMAAAVGLIKK18 pKa = 10.5DD19 pKa = 4.13ALMGDD24 pKa = 4.27DD25 pKa = 5.37KK26 pKa = 11.47PFATLSEE33 pKa = 4.31INEE36 pKa = 4.26CMVEE40 pKa = 4.28LTQHH44 pKa = 6.28YY45 pKa = 9.92IDD47 pKa = 4.17YY48 pKa = 10.77SPIEE52 pKa = 4.46FIPDD56 pKa = 3.57EE57 pKa = 4.71LKK59 pKa = 10.42DD60 pKa = 3.65AYY62 pKa = 10.88RR63 pKa = 11.84PFYY66 pKa = 11.08VGACEE71 pKa = 3.79LLSRR75 pKa = 11.84FSIPVTSLSTILSTSGSDD93 pKa = 3.81GFCMLLIEE101 pKa = 5.58DD102 pKa = 4.24EE103 pKa = 4.38

Molecular weight: 11.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7JW47|A0A7D7JW47_9CAUD Uncharacterized protein OS=Proteus phage 10 OX=2759182 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.26KK3 pKa = 10.15VPYY6 pKa = 8.86TLASTGEE13 pKa = 4.2INWNVIIRR21 pKa = 11.84ILATILTDD29 pKa = 4.22SSITINKK36 pKa = 8.56EE37 pKa = 3.52RR38 pKa = 11.84MLTSPSILNRR48 pKa = 11.84MPNGVKK54 pKa = 10.27RR55 pKa = 11.84SIHH58 pKa = 6.01RR59 pKa = 11.84LRR61 pKa = 11.84DD62 pKa = 3.18FAKK65 pKa = 10.11IHH67 pKa = 5.75LTNTNNRR74 pKa = 11.84DD75 pKa = 3.33TFKK78 pKa = 10.9RR79 pKa = 11.84LAMQHH84 pKa = 5.87GYY86 pKa = 9.78EE87 pKa = 4.07LSFTRR92 pKa = 11.84TIKK95 pKa = 10.59GCHH98 pKa = 4.8CHH100 pKa = 7.67IEE102 pKa = 3.93IKK104 pKa = 9.3TDD106 pKa = 2.86KK107 pKa = 11.13GIIKK111 pKa = 9.14FKK113 pKa = 10.71VMRR116 pKa = 11.84AHH118 pKa = 6.65NVQKK122 pKa = 9.99TSLL125 pKa = 3.53

Molecular weight: 14.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

232

0

232

68911

47

2034

297.0

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.172

0.965 ± 0.047

6.416 ± 0.115

5.99 ± 0.147

3.471 ± 0.085

6.649 ± 0.256

1.995 ± 0.072

6.857 ± 0.142

6.355 ± 0.15

8.518 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.078

5.813 ± 0.111

4.007 ± 0.114

2.972 ± 0.15

4.458 ± 0.099

6.88 ± 0.138

6.635 ± 0.112

7.18 ± 0.12

1.091 ± 0.049

4.158 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski