Amino acid dipepetide frequency for Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.23AlaAla: 7.23 ± 0.094	0.788AlaCys: 0.788 ± 0.022	3.985AlaAsp: 3.985 ± 0.049	5.026AlaGlu: 5.026 ± 0.06	2.897AlaPhe: 2.897 ± 0.04	5.385AlaGly: 5.385 ± 0.073	1.254AlaHis: 1.254 ± 0.029	6.764AlaIle: 6.764 ± 0.071	4.075AlaLys: 4.075 ± 0.057	8.37AlaLeu: 8.37 ± 0.091	1.549AlaMet: 1.549 ± 0.032	3.696AlaAsn: 3.696 ± 0.057	2.857AlaPro: 2.857 ± 0.046	4.153AlaGln: 4.153 ± 0.054	3.494AlaArg: 3.494 ± 0.048	4.83AlaSer: 4.83 ± 0.058	4.787AlaThr: 4.787 ± 0.054	5.471AlaVal: 5.471 ± 0.059	1.039AlaTrp: 1.039 ± 0.026	2.329AlaTyr: 2.329 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.612CysAla: 0.612 ± 0.018	0.156CysCys: 0.156 ± 0.01	0.539CysAsp: 0.539 ± 0.019	0.505CysGlu: 0.505 ± 0.017	0.415CysPhe: 0.415 ± 0.014	0.754CysGly: 0.754 ± 0.023	0.302CysHis: 0.302 ± 0.014	0.545CysIle: 0.545 ± 0.016	0.352CysLys: 0.352 ± 0.013	1.095CysLeu: 1.095 ± 0.023	0.142CysMet: 0.142 ± 0.009	0.388CysAsn: 0.388 ± 0.017	0.516CysPro: 0.516 ± 0.017	0.588CysGln: 0.588 ± 0.018	0.503CysArg: 0.503 ± 0.017	0.603CysSer: 0.603 ± 0.019	0.45CysThr: 0.45 ± 0.014	0.569CysVal: 0.569 ± 0.016	0.151CysTrp: 0.151 ± 0.009	0.335CysTyr: 0.335 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.507AspAla: 3.507 ± 0.049	0.493AspCys: 0.493 ± 0.018	2.243AspAsp: 2.243 ± 0.044	3.047AspGlu: 3.047 ± 0.046	2.36AspPhe: 2.36 ± 0.039	3.389AspGly: 3.389 ± 0.058	0.7AspHis: 0.7 ± 0.02	3.695AspIle: 3.695 ± 0.051	2.305AspLys: 2.305 ± 0.041	5.383AspLeu: 5.383 ± 0.053	0.735AspMet: 0.735 ± 0.022	2.225AspAsn: 2.225 ± 0.044	2.119AspPro: 2.119 ± 0.034	1.7AspGln: 1.7 ± 0.036	2.892AspArg: 2.892 ± 0.038	2.887AspSer: 2.887 ± 0.04	2.62AspThr: 2.62 ± 0.046	3.062AspVal: 3.062 ± 0.044	0.907AspTrp: 0.907 ± 0.022	1.834AspTyr: 1.834 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	5.087GluAla: 5.087 ± 0.06	0.462GluCys: 0.462 ± 0.016	2.63GluAsp: 2.63 ± 0.041	3.877GluGlu: 3.877 ± 0.055	2.416GluPhe: 2.416 ± 0.037	2.974GluGly: 2.974 ± 0.047	0.982GluHis: 0.982 ± 0.024	4.969GluIle: 4.969 ± 0.062	3.49GluLys: 3.49 ± 0.043	7.007GluLeu: 7.007 ± 0.074	1.225GluMet: 1.225 ± 0.028	2.805GluAsn: 2.805 ± 0.043	2.284GluPro: 2.284 ± 0.033	3.625GluGln: 3.625 ± 0.049	3.334GluArg: 3.334 ± 0.049	3.418GluSer: 3.418 ± 0.043	3.497GluThr: 3.497 ± 0.043	4.25GluVal: 4.25 ± 0.049	0.767GluTrp: 0.767 ± 0.019	1.991GluTyr: 1.991 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.159PheAla: 3.159 ± 0.049	0.514PheCys: 0.514 ± 0.016	2.175PheAsp: 2.175 ± 0.039	1.99PheGlu: 1.99 ± 0.031	1.677PhePhe: 1.677 ± 0.034	2.935PheGly: 2.935 ± 0.045	0.776PheHis: 0.776 ± 0.023	2.49PheIle: 2.49 ± 0.041	1.501PheLys: 1.501 ± 0.03	4.132PheLeu: 4.132 ± 0.055	0.708PheMet: 0.708 ± 0.022	1.859PheAsn: 1.859 ± 0.036	1.792PhePro: 1.792 ± 0.028	1.897PheGln: 1.897 ± 0.032	1.853PheArg: 1.853 ± 0.028	2.969PheSer: 2.969 ± 0.05	2.485PheThr: 2.485 ± 0.034	2.479PheVal: 2.479 ± 0.043	0.683PheTrp: 0.683 ± 0.021	1.406PheTyr: 1.406 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	4.707GlyAla: 4.707 ± 0.067	0.75GlyCys: 0.75 ± 0.022	3.291GlyAsp: 3.291 ± 0.051	4.143GlyGlu: 4.143 ± 0.048	2.917GlyPhe: 2.917 ± 0.041	4.826GlyGly: 4.826 ± 0.084	1.171GlyHis: 1.171 ± 0.035	5.083GlyIle: 5.083 ± 0.049	4.058GlyLys: 4.058 ± 0.056	7.017GlyLeu: 7.017 ± 0.061	1.409GlyMet: 1.409 ± 0.026	3.376GlyAsn: 3.376 ± 0.077	1.142GlyPro: 1.142 ± 0.029	2.938GlyGln: 2.938 ± 0.045	3.234GlyArg: 3.234 ± 0.042	4.017GlySer: 4.017 ± 0.057	3.847GlyThr: 3.847 ± 0.066	4.82GlyVal: 4.82 ± 0.059	1.108GlyTrp: 1.108 ± 0.026	2.415GlyTyr: 2.415 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	1.026HisAla: 1.026 ± 0.024	0.242HisCys: 0.242 ± 0.011	0.763HisAsp: 0.763 ± 0.019	0.968HisGlu: 0.968 ± 0.026	0.721HisPhe: 0.721 ± 0.02	1.119HisGly: 1.119 ± 0.023	0.618HisHis: 0.618 ± 0.02	1.227HisIle: 1.227 ± 0.029	0.866HisLys: 0.866 ± 0.021	2.246HisLeu: 2.246 ± 0.035	0.209HisMet: 0.209 ± 0.012	0.785HisAsn: 0.785 ± 0.022	1.336HisPro: 1.336 ± 0.029	1.2HisGln: 1.2 ± 0.024	1.056HisArg: 1.056 ± 0.027	1.146HisSer: 1.146 ± 0.028	0.994HisThr: 0.994 ± 0.026	0.786HisVal: 0.786 ± 0.021	0.366HisTrp: 0.366 ± 0.014	0.642HisTyr: 0.642 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.112IleAla: 7.112 ± 0.064	0.754IleCys: 0.754 ± 0.019	3.689IleAsp: 3.689 ± 0.046	4.23IleGlu: 4.23 ± 0.044	2.774IlePhe: 2.774 ± 0.04	4.491IleGly: 4.491 ± 0.057	1.296IleHis: 1.296 ± 0.029	4.132IleIle: 4.132 ± 0.057	3.24IleLys: 3.24 ± 0.043	7.0IleLeu: 7.0 ± 0.064	0.91IleMet: 0.91 ± 0.02	3.542IleAsn: 3.542 ± 0.052	3.682IlePro: 3.682 ± 0.052	3.242IleGln: 3.242 ± 0.046	3.175IleArg: 3.175 ± 0.039	4.723IleSer: 4.723 ± 0.052	4.239IleThr: 4.239 ± 0.058	4.307IleVal: 4.307 ± 0.05	0.894IleTrp: 0.894 ± 0.027	2.044IleTyr: 2.044 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.927LysAla: 3.927 ± 0.052	0.335LysCys: 0.335 ± 0.015	2.147LysAsp: 2.147 ± 0.035	2.721LysGlu: 2.721 ± 0.043	1.889LysPhe: 1.889 ± 0.038	2.563LysGly: 2.563 ± 0.037	0.829LysHis: 0.829 ± 0.022	3.603LysIle: 3.603 ± 0.049	2.296LysLys: 2.296 ± 0.045	5.567LysLeu: 5.567 ± 0.071	0.941LysMet: 0.941 ± 0.021	2.239LysAsn: 2.239 ± 0.04	2.414LysPro: 2.414 ± 0.042	2.781LysGln: 2.781 ± 0.044	2.348LysArg: 2.348 ± 0.037	3.08LysSer: 3.08 ± 0.045	3.0LysThr: 3.0 ± 0.038	3.133LysVal: 3.133 ± 0.042	0.507LysTrp: 0.507 ± 0.015	1.539LysTyr: 1.539 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	9.646LeuAla: 9.646 ± 0.083	0.985LeuCys: 0.985 ± 0.024	5.161LeuAsp: 5.161 ± 0.048	7.06LeuGlu: 7.06 ± 0.079	3.769LeuPhe: 3.769 ± 0.053	7.64LeuGly: 7.64 ± 0.074	1.906LeuHis: 1.906 ± 0.035	6.987LeuIle: 6.987 ± 0.068	5.424LeuLys: 5.424 ± 0.066	11.429LeuLeu: 11.429 ± 0.109	2.057LeuMet: 2.057 ± 0.031	4.737LeuAsn: 4.737 ± 0.055	5.724LeuPro: 5.724 ± 0.06	6.122LeuGln: 6.122 ± 0.071	5.571LeuArg: 5.571 ± 0.064	7.459LeuSer: 7.459 ± 0.069	6.584LeuThr: 6.584 ± 0.054	7.219LeuVal: 7.219 ± 0.068	1.478LeuTrp: 1.478 ± 0.032	2.706LeuTyr: 2.706 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	1.545MetAla: 1.545 ± 0.032	0.128MetCys: 0.128 ± 0.008	0.664MetAsp: 0.664 ± 0.021	0.968MetGlu: 0.968 ± 0.024	0.561MetPhe: 0.561 ± 0.017	1.266MetGly: 1.266 ± 0.028	0.219MetHis: 0.219 ± 0.011	1.111MetIle: 1.111 ± 0.027	0.94MetLys: 0.94 ± 0.025	1.927MetLeu: 1.927 ± 0.035	0.471MetMet: 0.471 ± 0.018	0.878MetAsn: 0.878 ± 0.021	0.857MetPro: 0.857 ± 0.019	0.973MetGln: 0.973 ± 0.024	0.918MetArg: 0.918 ± 0.023	1.318MetSer: 1.318 ± 0.028	1.269MetThr: 1.269 ± 0.027	1.219MetVal: 1.219 ± 0.029	0.155MetTrp: 0.155 ± 0.01	0.384MetTyr: 0.384 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.172AsnAla: 3.172 ± 0.046	0.485AsnCys: 0.485 ± 0.016	1.827AsnAsp: 1.827 ± 0.057	1.89AsnGlu: 1.89 ± 0.03	2.122AsnPhe: 2.122 ± 0.035	2.851AsnGly: 2.851 ± 0.056	0.965AsnHis: 0.965 ± 0.025	3.246AsnIle: 3.246 ± 0.055	1.834AsnLys: 1.834 ± 0.033	5.787AsnLeu: 5.787 ± 0.065	0.646AsnMet: 0.646 ± 0.02	2.537AsnAsn: 2.537 ± 0.052	3.051AsnPro: 3.051 ± 0.041	2.897AsnGln: 2.897 ± 0.05	2.375AsnArg: 2.375 ± 0.035	3.25AsnSer: 3.25 ± 0.047	2.581AsnThr: 2.581 ± 0.06	2.352AsnVal: 2.352 ± 0.039	0.801AsnTrp: 0.801 ± 0.021	1.679AsnTyr: 1.679 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	3.113ProAla: 3.113 ± 0.049	0.348ProCys: 0.348 ± 0.015	2.911ProAsp: 2.911 ± 0.044	3.874ProGlu: 3.874 ± 0.054	1.656ProPhe: 1.656 ± 0.03	3.019ProGly: 3.019 ± 0.044	0.91ProHis: 0.91 ± 0.024	3.175ProIle: 3.175 ± 0.044	2.098ProLys: 2.098 ± 0.036	4.556ProLeu: 4.556 ± 0.052	0.703ProMet: 0.703 ± 0.021	2.313ProAsn: 2.313 ± 0.033	2.291ProPro: 2.291 ± 0.045	2.842ProGln: 2.842 ± 0.046	1.684ProArg: 1.684 ± 0.031	2.972ProSer: 2.972 ± 0.044	2.965ProThr: 2.965 ± 0.052	3.273ProVal: 3.273 ± 0.04	0.595ProTrp: 0.595 ± 0.019	1.293ProTyr: 1.293 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	4.972GlnAla: 4.972 ± 0.06	0.344GlnCys: 0.344 ± 0.013	2.251GlnAsp: 2.251 ± 0.036	3.752GlnGlu: 3.752 ± 0.048	1.812GlnPhe: 1.812 ± 0.03	3.42GlnGly: 3.42 ± 0.055	0.876GlnHis: 0.876 ± 0.023	3.87GlnIle: 3.87 ± 0.045	2.859GlnLys: 2.859 ± 0.037	6.137GlnLeu: 6.137 ± 0.071	1.108GlnMet: 1.108 ± 0.021	2.33GlnAsn: 2.33 ± 0.036	2.752GlnPro: 2.752 ± 0.041	4.074GlnGln: 4.074 ± 0.071	2.9GlnArg: 2.9 ± 0.042	3.074GlnSer: 3.074 ± 0.043	3.079GlnThr: 3.079 ± 0.045	3.931GlnVal: 3.931 ± 0.046	0.675GlnTrp: 0.675 ± 0.02	1.339GlnTyr: 1.339 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	3.034ArgAla: 3.034 ± 0.042	0.475ArgCys: 0.475 ± 0.017	2.481ArgAsp: 2.481 ± 0.035	3.115ArgGlu: 3.115 ± 0.044	2.171ArgPhe: 2.171 ± 0.032	3.014ArgGly: 3.014 ± 0.043	1.008ArgHis: 1.008 ± 0.024	3.315ArgIle: 3.315 ± 0.044	2.212ArgLys: 2.212 ± 0.037	5.973ArgLeu: 5.973 ± 0.052	0.954ArgMet: 0.954 ± 0.021	2.203ArgAsn: 2.203 ± 0.033	2.033ArgPro: 2.033 ± 0.037	3.305ArgGln: 3.305 ± 0.044	3.019ArgArg: 3.019 ± 0.049	3.189ArgSer: 3.189 ± 0.042	2.543ArgThr: 2.543 ± 0.041	3.392ArgVal: 3.392 ± 0.045	0.789ArgTrp: 0.789 ± 0.024	1.884ArgTyr: 1.884 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.509SerAla: 4.509 ± 0.059	0.598SerCys: 0.598 ± 0.019	3.125SerAsp: 3.125 ± 0.038	3.603SerGlu: 3.603 ± 0.055	2.604SerPhe: 2.604 ± 0.04	4.637SerGly: 4.637 ± 0.049	1.385SerHis: 1.385 ± 0.031	4.029SerIle: 4.029 ± 0.051	2.6SerLys: 2.6 ± 0.04	7.507SerLeu: 7.507 ± 0.083	1.13SerMet: 1.13 ± 0.027	2.826SerAsn: 2.826 ± 0.053	3.5SerPro: 3.5 ± 0.049	3.876SerGln: 3.876 ± 0.053	3.232SerArg: 3.232 ± 0.046	4.522SerSer: 4.522 ± 0.057	3.638SerThr: 3.638 ± 0.05	4.147SerVal: 4.147 ± 0.042	0.955SerTrp: 0.955 ± 0.024	1.877SerTyr: 1.877 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.952ThrAla: 4.952 ± 0.059	0.452ThrCys: 0.452 ± 0.016	2.761ThrAsp: 2.761 ± 0.042	3.264ThrGlu: 3.264 ± 0.046	2.19ThrPhe: 2.19 ± 0.039	4.411ThrGly: 4.411 ± 0.062	1.054ThrHis: 1.054 ± 0.024	3.999ThrIle: 3.999 ± 0.061	2.493ThrLys: 2.493 ± 0.038	6.372ThrLeu: 6.372 ± 0.066	0.757ThrMet: 0.757 ± 0.023	2.606ThrAsn: 2.606 ± 0.047	3.562ThrPro: 3.562 ± 0.055	3.105ThrGln: 3.105 ± 0.047	2.406ThrArg: 2.406 ± 0.036	3.719ThrSer: 3.719 ± 0.051	3.722ThrThr: 3.722 ± 0.058	4.248ThrVal: 4.248 ± 0.059	0.742ThrTrp: 0.742 ± 0.023	1.6ThrTyr: 1.6 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	5.751ValAla: 5.751 ± 0.06	0.657ValCys: 0.657 ± 0.019	3.4ValAsp: 3.4 ± 0.044	4.386ValGlu: 4.386 ± 0.051	2.559ValPhe: 2.559 ± 0.04	4.594ValGly: 4.594 ± 0.056	1.026ValHis: 1.026 ± 0.024	4.621ValIle: 4.621 ± 0.056	3.3ValLys: 3.3 ± 0.043	6.568ValLeu: 6.568 ± 0.064	1.39ValMet: 1.39 ± 0.028	3.126ValAsn: 3.126 ± 0.046	2.813ValPro: 2.813 ± 0.039	2.905ValGln: 2.905 ± 0.039	3.247ValArg: 3.247 ± 0.048	4.253ValSer: 4.253 ± 0.054	3.945ValThr: 3.945 ± 0.054	4.919ValVal: 4.919 ± 0.055	0.853ValTrp: 0.853 ± 0.023	1.874ValTyr: 1.874 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	0.849TrpAla: 0.849 ± 0.025	0.162TrpCys: 0.162 ± 0.01	0.63TrpAsp: 0.63 ± 0.022	0.928TrpGlu: 0.928 ± 0.024	0.565TrpPhe: 0.565 ± 0.018	0.971TrpGly: 0.971 ± 0.026	0.349TrpHis: 0.349 ± 0.013	0.813TrpIle: 0.813 ± 0.022	0.608TrpLys: 0.608 ± 0.016	1.934TrpLeu: 1.934 ± 0.036	0.309TrpMet: 0.309 ± 0.014	0.652TrpAsn: 0.652 ± 0.02	0.282TrpPro: 0.282 ± 0.014	1.251TrpGln: 1.251 ± 0.027	0.879TrpArg: 0.879 ± 0.025	0.856TrpSer: 0.856 ± 0.022	0.598TrpThr: 0.598 ± 0.019	0.969TrpVal: 0.969 ± 0.021	0.248TrpTrp: 0.248 ± 0.011	0.418TrpTyr: 0.418 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.07TyrAla: 2.07 ± 0.031	0.391TyrCys: 0.391 ± 0.014	1.43TyrAsp: 1.43 ± 0.03	1.748TyrGlu: 1.748 ± 0.034	1.403TyrPhe: 1.403 ± 0.029	2.008TyrGly: 2.008 ± 0.035	0.692TyrHis: 0.692 ± 0.02	1.799TyrIle: 1.799 ± 0.031	1.308TyrLys: 1.308 ± 0.027	3.657TyrLeu: 3.657 ± 0.045	0.42TyrMet: 0.42 ± 0.016	1.227TyrAsn: 1.227 ± 0.027	1.586TyrPro: 1.586 ± 0.026	2.081TyrGln: 2.081 ± 0.037	1.986TyrArg: 1.986 ± 0.035	2.013TyrSer: 2.013 ± 0.034	1.572TyrThr: 1.572 ± 0.03	1.642TyrVal: 1.642 ± 0.029	0.557TyrTrp: 0.557 ± 0.019	1.098TyrTyr: 1.098 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6071 proteins (1955973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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