Gammaproteobacteria bacterium LSUCC0112

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8UXX4|A0A4Y8UXX4_9GAMM Chemotaxis protein CheW OS=Gammaproteobacteria bacterium LSUCC0112 OX=1778437 GN=E3V39_08910 PE=4 SV=1
MM1 pKa = 7.6RR2 pKa = 11.84KK3 pKa = 9.24SVPSFNFLTWLYY15 pKa = 10.97GFFVCFSFEE24 pKa = 3.99THH26 pKa = 6.51AQTQNYY32 pKa = 9.67NIDD35 pKa = 3.67DD36 pKa = 3.88TSRR39 pKa = 11.84AAVQVIVDD47 pKa = 3.77NATGSGTLIIVDD59 pKa = 4.12GKK61 pKa = 8.74PTVFTNRR68 pKa = 11.84HH69 pKa = 3.96VVEE72 pKa = 5.25GFDD75 pKa = 3.27QATIAVLVDD84 pKa = 3.88PNAPAEE90 pKa = 4.08PMFIANLVGFSQEE103 pKa = 3.52YY104 pKa = 10.78DD105 pKa = 3.44FAVYY109 pKa = 9.26TLATDD114 pKa = 4.09LQGNTVSAQQLRR126 pKa = 11.84DD127 pKa = 3.03GSFGVRR133 pKa = 11.84FPDD136 pKa = 3.85ITVQDD141 pKa = 3.89IDD143 pKa = 3.97NKK145 pKa = 10.58GSDD148 pKa = 3.57VRR150 pKa = 11.84RR151 pKa = 11.84GDD153 pKa = 3.41TVGIFGYY160 pKa = 9.86PGIGDD165 pKa = 4.25DD166 pKa = 3.83EE167 pKa = 4.54LVYY170 pKa = 8.55TTGIISSVQFGEE182 pKa = 4.4YY183 pKa = 9.57KK184 pKa = 10.68GEE186 pKa = 4.11RR187 pKa = 11.84LPMWYY192 pKa = 8.04RR193 pKa = 11.84TNAEE197 pKa = 4.12MSPGNSGGMALNGRR211 pKa = 11.84GEE213 pKa = 4.65FIGIPTSVRR222 pKa = 11.84TEE224 pKa = 3.76NLTGGRR230 pKa = 11.84LGSLLAVPFVMAILEE245 pKa = 4.38DD246 pKa = 4.23DD247 pKa = 4.09EE248 pKa = 5.75GLVASWSGVDD258 pKa = 3.43SSIAASEE265 pKa = 4.13LNILQEE271 pKa = 4.28PGFGSVTLAPSEE283 pKa = 3.96LGEE286 pKa = 4.13PFFAEE291 pKa = 5.08LISGGSVDD299 pKa = 4.96VSYY302 pKa = 11.2LGNNCVGFAASNPDD316 pKa = 3.26YY317 pKa = 10.8RR318 pKa = 11.84IALTDD323 pKa = 4.13DD324 pKa = 3.64MTDD327 pKa = 3.19MTILFLAEE335 pKa = 4.76DD336 pKa = 4.0EE337 pKa = 4.91GADD340 pKa = 3.23TTLIVNAPDD349 pKa = 4.62GRR351 pKa = 11.84WHH353 pKa = 6.97CNDD356 pKa = 4.85DD357 pKa = 3.69EE358 pKa = 6.58SDD360 pKa = 3.64DD361 pKa = 4.07SRR363 pKa = 11.84DD364 pKa = 3.57PGLLFTNATSGQYY377 pKa = 10.27DD378 pKa = 3.28IWIGSYY384 pKa = 10.12DD385 pKa = 3.56QNDD388 pKa = 3.75AVSGVLAVVDD398 pKa = 5.32ASDD401 pKa = 3.6FDD403 pKa = 4.76NIEE406 pKa = 4.21SSVGAQGTALDD417 pKa = 4.65FAADD421 pKa = 4.43PYY423 pKa = 11.23YY424 pKa = 11.27GSADD428 pKa = 3.41LAAGFTPDD436 pKa = 3.29PHH438 pKa = 7.36VVSVSAGGAVDD449 pKa = 3.92VSIAGYY455 pKa = 10.71GNEE458 pKa = 3.95CRR460 pKa = 11.84GFASSAPDD468 pKa = 3.41YY469 pKa = 10.89KK470 pKa = 10.48VTLSGDD476 pKa = 3.53SALLQLYY483 pKa = 8.33FVASSIGDD491 pKa = 3.79DD492 pKa = 3.11ATLIINNPEE501 pKa = 4.08GTWLCNDD508 pKa = 4.62DD509 pKa = 5.26APSSLNPGITINNPVAGRR527 pKa = 11.84YY528 pKa = 8.99DD529 pKa = 3.16IWVGAYY535 pKa = 9.8AQGEE539 pKa = 4.57FINGEE544 pKa = 3.88LRR546 pKa = 11.84ITEE549 pKa = 4.6LSTTVPP555 pKa = 3.55

Molecular weight: 58.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8V434|A0A4Y8V434_9GAMM DsbC domain-containing protein OS=Gammaproteobacteria bacterium LSUCC0112 OX=1778437 GN=E3V39_07115 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLIKK11 pKa = 10.3RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVIKK32 pKa = 10.33RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 9.71LTAA44 pKa = 4.28

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3053

0

3053

1073988

23

4975

351.8

38.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.015 ± 0.05

0.94 ± 0.015

5.738 ± 0.033

5.427 ± 0.037

3.907 ± 0.032

7.488 ± 0.048

2.19 ± 0.026

5.692 ± 0.034

3.231 ± 0.036

10.63 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.649 ± 0.023

3.773 ± 0.038

4.533 ± 0.035

4.516 ± 0.043

5.951 ± 0.05

6.527 ± 0.037

5.572 ± 0.071

7.238 ± 0.034

1.324 ± 0.017

2.654 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski