Phytophthora fragariae

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34421 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A3WLA1|A0A6A3WLA1_9STRA Uncharacterized protein OS=Phytophthora fragariae OX=53985 GN=PF002_g27136 PE=4 SV=1
MM1 pKa = 7.16VNVLRR6 pKa = 11.84TTSALAMAATTFGLANAGSFEE27 pKa = 4.39CTGINYY33 pKa = 9.54NIRR36 pKa = 11.84AGPDD40 pKa = 2.57WATADD45 pKa = 4.08VKK47 pKa = 10.81CKK49 pKa = 9.94PSSQIATEE57 pKa = 4.34LSTLKK62 pKa = 10.88GVTDD66 pKa = 4.4TIRR69 pKa = 11.84LYY71 pKa = 11.34SLTDD75 pKa = 3.55CDD77 pKa = 3.0QATAVVPAAIEE88 pKa = 4.13AGLKK92 pKa = 9.83IEE94 pKa = 4.43LGMWVDD100 pKa = 3.67SATSSFEE107 pKa = 3.84AEE109 pKa = 3.71KK110 pKa = 10.82AAFQTLLEE118 pKa = 4.34TGIVTADD125 pKa = 3.8NIVGIHH131 pKa = 5.63VGSEE135 pKa = 3.25AVYY138 pKa = 10.56RR139 pKa = 11.84GDD141 pKa = 2.94VMAAVAISNLEE152 pKa = 4.18EE153 pKa = 4.55IRR155 pKa = 11.84TLCQANSGAASIPLTIADD173 pKa = 3.89IGDD176 pKa = 3.75TYY178 pKa = 11.24SAYY181 pKa = 9.91PEE183 pKa = 4.32MIQAVDD189 pKa = 3.78YY190 pKa = 11.34VSANYY195 pKa = 9.71FPFWEE200 pKa = 4.59KK201 pKa = 11.15VDD203 pKa = 3.35IDD205 pKa = 3.6GAADD209 pKa = 3.25HH210 pKa = 6.59FYY212 pKa = 11.13EE213 pKa = 4.57RR214 pKa = 11.84FSALVEE220 pKa = 4.16TASTYY225 pKa = 10.07SKK227 pKa = 10.62EE228 pKa = 4.31VIVGEE233 pKa = 4.43TGWASNGTDD242 pKa = 3.57ADD244 pKa = 3.89ASPATAANAAKK255 pKa = 10.04YY256 pKa = 9.66FHH258 pKa = 7.44DD259 pKa = 5.13FYY261 pKa = 11.39TLAEE265 pKa = 4.66EE266 pKa = 4.29KK267 pKa = 10.79DD268 pKa = 3.24LMYY271 pKa = 10.32FYY273 pKa = 10.58FSAFDD278 pKa = 4.17EE279 pKa = 4.52PWKK282 pKa = 10.71LATLEE287 pKa = 4.08ANEE290 pKa = 4.12TVEE293 pKa = 5.12AYY295 pKa = 10.4FGLFTQEE302 pKa = 4.22GVLKK306 pKa = 10.51DD307 pKa = 3.94VISAATASLDD317 pKa = 3.65GSDD320 pKa = 3.87SATITAVGADD330 pKa = 4.01SSTSDD335 pKa = 3.0ATTTASAASTSADD348 pKa = 3.09NSTLITTTSVYY359 pKa = 9.66ATSSDD364 pKa = 4.2DD365 pKa = 3.69DD366 pKa = 5.13DD367 pKa = 6.08SIQGEE372 pKa = 4.24ATSDD376 pKa = 3.11VDD378 pKa = 3.68ASSDD382 pKa = 3.76EE383 pKa = 4.29EE384 pKa = 4.52TPSDD388 pKa = 4.89DD389 pKa = 5.62GEE391 pKa = 6.28DD392 pKa = 3.63DD393 pKa = 3.9TTDD396 pKa = 3.17STTQTSTTTTSSSHH410 pKa = 6.64KK411 pKa = 10.27DD412 pKa = 3.09CGMM415 pKa = 3.95

Molecular weight: 43.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A3YZK2|A0A6A3YZK2_9STRA Uncharacterized protein OS=Phytophthora fragariae OX=53985 GN=PF001_g8246 PE=4 SV=1
MM1 pKa = 7.51LRR3 pKa = 11.84GCRR6 pKa = 11.84RR7 pKa = 11.84SLRR10 pKa = 11.84LLRR13 pKa = 11.84VIRR16 pKa = 11.84MLLGSRR22 pKa = 11.84RR23 pKa = 11.84VLRR26 pKa = 11.84SLWIARR32 pKa = 11.84VIRR35 pKa = 11.84RR36 pKa = 11.84SRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84LSLLFAWSRR50 pKa = 11.84RR51 pKa = 11.84VGVMLGRR58 pKa = 11.84ASGPRR63 pKa = 11.84ALGAARR69 pKa = 11.84TMIWLSVVMLGGPP82 pKa = 3.5

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33589

832

34421

11269359

12

10552

327.4

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.578 ± 0.016

1.656 ± 0.008

5.77 ± 0.01

6.622 ± 0.019

3.512 ± 0.01

6.155 ± 0.015

2.307 ± 0.007

3.709 ± 0.009

4.919 ± 0.016

9.179 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.007

3.25 ± 0.007

5.044 ± 0.012

4.155 ± 0.011

6.691 ± 0.017

8.236 ± 0.019

5.912 ± 0.015

7.182 ± 0.014

1.244 ± 0.005

2.451 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski