Rhizobiales bacterium GAS113

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; unclassified Hyphomicrobiales

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1ESL9|A0A1H1ESL9_9RHIZ Hydroxymethylpyrimidine kinase /phosphomethylpyrimidine kinase OS=Rhizobiales bacterium GAS113 OX=1884352 GN=SAMN05519103_00074 PE=4 SV=1
MM1 pKa = 7.84SDD3 pKa = 4.51PITNLHH9 pKa = 5.28YY10 pKa = 9.83TSGANLVNGQYY21 pKa = 10.56VPAADD26 pKa = 4.2GFNLADD32 pKa = 4.03VNSLDD37 pKa = 4.11QLNSLPSGVKK47 pKa = 10.07GLVYY51 pKa = 11.0LNMTGGATAAFQSAVSLYY69 pKa = 10.06IGNPKK74 pKa = 10.43LYY76 pKa = 10.52GFYY79 pKa = 10.6LVDD82 pKa = 4.16EE83 pKa = 5.32PDD85 pKa = 3.85PSLVPAANLKK95 pKa = 10.5SEE97 pKa = 4.63ADD99 pKa = 3.65WIHH102 pKa = 5.96AQLPGAITFTVLLNLGTPTNPTYY125 pKa = 11.33LNTYY129 pKa = 9.91NSANTDD135 pKa = 2.57IDD137 pKa = 5.01LFGLDD142 pKa = 4.68PYY144 pKa = 10.52PIRR147 pKa = 11.84PQFTNGVNYY156 pKa = 9.21TIIPAAVNAAEE167 pKa = 4.31AAGIAQSQIVPVYY180 pKa = 10.25QAFGDD185 pKa = 4.13SASYY189 pKa = 9.04TLPTASQEE197 pKa = 4.15QQILTTWGSVVPTPAFDD214 pKa = 3.91YY215 pKa = 10.38AYY217 pKa = 10.8SWGSQNGSLSLSGTPALQQIFAIHH241 pKa = 5.83NQPVSNTTVLNSVIEE256 pKa = 4.15APAVGISNIGNVVTITIGFSGTATVSGAPTLSLNDD291 pKa = 3.88NGTATYY297 pKa = 10.84VGGSGTNALVFSYY310 pKa = 10.29QVASSDD316 pKa = 3.22IDD318 pKa = 3.83VSSLAVTSVNLNGGTIHH335 pKa = 7.78DD336 pKa = 5.18AIGNNANLSLNAVTQGGPQIVTTVAKK362 pKa = 10.27DD363 pKa = 3.16QSLFSGISDD372 pKa = 3.7LTNYY376 pKa = 8.88PPHH379 pKa = 6.59NALAVGPNNIVMAEE393 pKa = 4.32GSRR396 pKa = 11.84IEE398 pKa = 4.14WTDD401 pKa = 3.27LTGGSATTQSVYY413 pKa = 11.35AFFGSLGTTATNSLFDD429 pKa = 3.67ARR431 pKa = 11.84AAYY434 pKa = 10.37DD435 pKa = 4.12SVNHH439 pKa = 6.52RR440 pKa = 11.84YY441 pKa = 8.96VVTMDD446 pKa = 4.09NIGSGGTISNVDD458 pKa = 3.02IAVSKK463 pKa = 10.7DD464 pKa = 3.33SNPADD469 pKa = 2.82GWYY472 pKa = 10.25FSSLNTSVTINGQLTASDD490 pKa = 3.97QPAVAVDD497 pKa = 3.51GTNIYY502 pKa = 8.53ITTPQYY508 pKa = 11.34NVNTSGWQGTEE519 pKa = 3.3LWVIGDD525 pKa = 3.61TAGAGGGIYY534 pKa = 10.35NGGTLTVAANQVALPGGSIFKK555 pKa = 10.34VAAGNNGKK563 pKa = 9.87SYY565 pKa = 10.95YY566 pKa = 10.53AGAYY570 pKa = 7.31STGDD574 pKa = 3.29HH575 pKa = 5.71TVVTVQTYY583 pKa = 11.2DD584 pKa = 3.05LGTNTFGTASTIALGNNDD602 pKa = 3.62QGNGGAEE609 pKa = 3.96LTAQQQGTNLLLDD622 pKa = 4.83AGDD625 pKa = 3.91STLQSLAYY633 pKa = 10.13AGGYY637 pKa = 10.16LYY639 pKa = 10.9GLSEE643 pKa = 4.4VKK645 pKa = 10.5PFGSGVPEE653 pKa = 3.84LHH655 pKa = 6.18WFKK658 pKa = 11.36LDD660 pKa = 3.38VSNPNNPIVVAQGDD674 pKa = 3.76VSGAAIGTNVATFDD688 pKa = 3.55GSIAVDD694 pKa = 3.71GAGDD698 pKa = 4.34AIINFTASGPNMYY711 pKa = 9.66PADD714 pKa = 3.75YY715 pKa = 10.26YY716 pKa = 10.91VYY718 pKa = 10.42HH719 pKa = 6.9GAADD723 pKa = 3.7PTGSFGAPVLYY734 pKa = 10.02QASTGFFNSGNGAGTQDD751 pKa = 2.5WGTRR755 pKa = 11.84SSAIADD761 pKa = 3.68PNNPHH766 pKa = 6.0SFWVSGEE773 pKa = 3.98YY774 pKa = 9.63VANGWWQTSAAQVAVQTVTAIIAPVVSSIAASGPGITGGNGDD816 pKa = 4.08LNAGKK821 pKa = 10.4VVTLTVNFSAPVIVNTTGGSPMLSLNDD848 pKa = 4.07GGSATYY854 pKa = 10.5AGGSGTSALTFSYY867 pKa = 10.01TVAAGQNTPDD877 pKa = 3.94LVVSALSLNGATIQGAAANNADD899 pKa = 4.52LSGATNSNPAGILQIDD915 pKa = 3.86TTSPVVTITSMGGSVNQATQTVTGTVTDD943 pKa = 4.1ADD945 pKa = 3.97GGTTGTTVTLFDD957 pKa = 3.63GTAQAGTATVQADD970 pKa = 4.28GSWSASVTLVNGTNTLTAKK989 pKa = 9.82DD990 pKa = 3.83TDD992 pKa = 3.93PAGNTGTSNGVTYY1005 pKa = 9.95TLTSPAPVVSSIATSGPGITGGNGDD1030 pKa = 4.08LNAGKK1035 pKa = 10.4VVTLTVNFSAPVTVNTTGGSPMLSLNDD1062 pKa = 4.02GGSASYY1068 pKa = 10.89AGGSGTSALTFSYY1081 pKa = 9.99TVAVGQNTPDD1091 pKa = 3.46LTVSALSLNGATIQDD1106 pKa = 3.56AAANNADD1113 pKa = 4.24LSGATNSNPAGILQIDD1129 pKa = 3.86TTSPVVTITSMGGSVNQATQTVTGTVTDD1157 pKa = 4.1ADD1159 pKa = 3.97GGTTGTTVTLFDD1171 pKa = 3.63GTAQAGTATVQADD1184 pKa = 4.13GSWNASVTLVNGTNTLTAKK1203 pKa = 9.82DD1204 pKa = 3.83TDD1206 pKa = 3.93PAGNTGTSNGVTYY1219 pKa = 9.95TLTSPAPVVSSIATSGPGISGGNGDD1244 pKa = 4.28LNAGKK1249 pKa = 10.4VVTLTVNFSAPVTVNTTGGSPTLSLNDD1276 pKa = 4.11GGSATYY1282 pKa = 10.5AGGSGTSALTFSYY1295 pKa = 10.01TVAAGQNSPDD1305 pKa = 3.62LTVSALTLNGATIQDD1320 pKa = 3.72AAATNADD1327 pKa = 3.69LSGATNSNPAGILQIDD1343 pKa = 3.86TTSPVVTITSMGGSVNQATQTLTGTVTDD1371 pKa = 4.47ADD1373 pKa = 3.97GGTTGTTVTLFDD1385 pKa = 3.63GTAQAGTATVQADD1398 pKa = 4.28GSWSASVTLVNGTNTLTAKK1417 pKa = 9.82DD1418 pKa = 3.83TDD1420 pKa = 3.93PAGNTGTSNGVTYY1433 pKa = 9.95TLTSPAPVVSSIATSGPGISGGNGDD1458 pKa = 4.28LNAGKK1463 pKa = 10.4VVTLTVNFSAPVTVNTTGGSPTLSLNDD1490 pKa = 4.11GGSATYY1496 pKa = 10.5AGGSGTSALTFSYY1509 pKa = 10.01TVAAGQNTPDD1519 pKa = 3.69LTVSALTLNGATIQDD1534 pKa = 3.72AAATNADD1541 pKa = 3.69LSGATNSNPAGILQIDD1557 pKa = 4.01TTSPTLAIATIASNNVINASTASAGFAISGTSTDD1591 pKa = 3.89AEE1593 pKa = 4.16NGQIVTVNIANSSNTVVDD1611 pKa = 4.39SYY1613 pKa = 9.54TTTDD1617 pKa = 3.3QGNAWSVNVTSAQATALADD1636 pKa = 3.29GRR1638 pKa = 11.84YY1639 pKa = 7.01TVTANVADD1647 pKa = 4.03QAGNAAPQASGALTVDD1663 pKa = 3.28EE1664 pKa = 5.42DD1665 pKa = 4.03KK1666 pKa = 11.31AAEE1669 pKa = 4.22APALTIANTALTVPAGGSVPLGITATQLDD1698 pKa = 3.99SDD1700 pKa = 4.51DD1701 pKa = 4.54RR1702 pKa = 11.84LTVTISGVPKK1712 pKa = 10.3YY1713 pKa = 10.66EE1714 pKa = 4.94SITAPSGDD1722 pKa = 3.48SVTSKK1727 pKa = 10.55RR1728 pKa = 11.84LSNGTYY1734 pKa = 8.61TWTITEE1740 pKa = 4.05NAGAAGTPLAGLMLKK1755 pKa = 10.27SSYY1758 pKa = 10.52AGSGHH1763 pKa = 7.41PIATFRR1769 pKa = 11.84VTASNATSGEE1779 pKa = 4.38TGTSTARR1786 pKa = 11.84SITVTDD1792 pKa = 4.63PPATTSATSLNSVAPDD1808 pKa = 3.88LDD1810 pKa = 3.64AAAATSPASVCRR1822 pKa = 11.84LAMVFNQFMAAGFQNDD1838 pKa = 3.42QNAAGQMTSLPQISSSLEE1856 pKa = 3.59DD1857 pKa = 3.33LAFLSRR1863 pKa = 11.84PHH1865 pKa = 6.66HH1866 pKa = 5.88SAA1868 pKa = 3.15

Molecular weight: 185.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1IAA7|A0A1H1IAA7_9RHIZ Uncharacterized protein OS=Rhizobiales bacterium GAS113 OX=1884352 GN=SAMN05519103_02209 PE=3 SV=1
MM1 pKa = 7.68GSGSLLISIATGAVAVVLVLGLINMLRR28 pKa = 11.84GGSPNRR34 pKa = 11.84SQNLMRR40 pKa = 11.84LRR42 pKa = 11.84VVLQAVAILLIVLVLWWRR60 pKa = 11.84GHH62 pKa = 4.79

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9038

0

9038

2831079

29

12478

313.2

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.059 ± 0.039

0.908 ± 0.009

5.26 ± 0.023

5.419 ± 0.028

3.709 ± 0.019

8.636 ± 0.035

2.06 ± 0.014

5.124 ± 0.018

3.135 ± 0.023

10.439 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.014

2.406 ± 0.019

5.375 ± 0.023

3.071 ± 0.016

7.531 ± 0.033

5.621 ± 0.022

5.171 ± 0.036

7.27 ± 0.02

1.337 ± 0.011

2.164 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski