Streptococcus phage Javan233

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A9A7|A0A4D6A9A7_9CAUD Phage_Mu_F domain-containing protein OS=Streptococcus phage Javan233 OX=2548059 GN=Javan233_0038 PE=4 SV=1
MM1 pKa = 7.45NKK3 pKa = 9.44QEE5 pKa = 4.37IMSGMEE11 pKa = 4.6SIMQIAMCYY20 pKa = 8.57CHH22 pKa = 7.03PSVPDD27 pKa = 3.11VDD29 pKa = 4.53DD30 pKa = 3.23WHH32 pKa = 6.76AVHH35 pKa = 7.02SIAEE39 pKa = 4.33TIYY42 pKa = 11.27DD43 pKa = 4.08EE44 pKa = 4.67LKK46 pKa = 10.58KK47 pKa = 11.03DD48 pKa = 3.83EE49 pKa = 5.16DD50 pKa = 4.32DD51 pKa = 3.78EE52 pKa = 4.54

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A428|A0A4D6A428_9CAUD Major capsid protein OS=Streptococcus phage Javan233 OX=2548059 GN=Javan233_0041 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.37VGPRR6 pKa = 11.84KK7 pKa = 9.53PSFKK11 pKa = 10.16RR12 pKa = 11.84SVKK15 pKa = 10.35ARR17 pKa = 11.84TTGKK21 pKa = 10.08VKK23 pKa = 10.52NQARR27 pKa = 11.84SAINPVYY34 pKa = 10.36SKK36 pKa = 11.22KK37 pKa = 11.0GMGWVNNPKK46 pKa = 9.76KK47 pKa = 10.21AAYY50 pKa = 9.61NKK52 pKa = 9.75VYY54 pKa = 10.96NKK56 pKa = 7.96TTFSVFDD63 pKa = 5.28LIDD66 pKa = 3.99EE67 pKa = 4.25NTIGCGCGFIVFIIFIFVMRR87 pKa = 11.84FLYY90 pKa = 10.42HH91 pKa = 7.13ALDD94 pKa = 3.74FLFF97 pKa = 5.12

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11384

38

1653

192.9

21.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.273 ± 0.45

0.553 ± 0.075

6.29 ± 0.357

7.238 ± 0.588

4.014 ± 0.238

5.859 ± 0.423

1.256 ± 0.164

6.773 ± 0.302

8.152 ± 0.399

8.029 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.206

6.087 ± 0.426

2.635 ± 0.189

4.067 ± 0.266

3.768 ± 0.291

6.843 ± 0.42

6.738 ± 0.654

6.571 ± 0.301

1.37 ± 0.185

4.146 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski