Lentibacillus salicampi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lentibacillus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9A7L5|A0A4Y9A7L5_9BACI Ectoine/hydroxyectoine ABC transporter permease subunit EhuD OS=Lentibacillus salicampi OX=175306 GN=ehuD PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.52LVGLLLVVLLFALAGCGSAQNEE24 pKa = 4.41GDD26 pKa = 4.29DD27 pKa = 4.31SDD29 pKa = 4.89SGSDD33 pKa = 4.27DD34 pKa = 3.57GGGDD38 pKa = 3.41EE39 pKa = 4.82ASSSEE44 pKa = 4.14EE45 pKa = 3.64VRR47 pKa = 11.84ITLGTGGMSGTYY59 pKa = 9.68YY60 pKa = 10.35PLGVAMTEE68 pKa = 4.23QIFSNAEE75 pKa = 3.73GVSQARR81 pKa = 11.84AVSTGASVTNAQEE94 pKa = 3.93LAEE97 pKa = 4.35GKK99 pKa = 9.2YY100 pKa = 9.23QAVLVQNDD108 pKa = 3.0IAYY111 pKa = 9.62YY112 pKa = 9.94AVNGEE117 pKa = 4.18TLADD121 pKa = 3.95FEE123 pKa = 5.55GNTVDD128 pKa = 4.96NMAGMTSLYY137 pKa = 10.11PEE139 pKa = 5.76DD140 pKa = 3.57IQVVTTADD148 pKa = 3.27SDD150 pKa = 3.97IEE152 pKa = 4.18SLEE155 pKa = 3.98DD156 pKa = 3.52LKK158 pKa = 11.51GKK160 pKa = 9.85NVAVGDD166 pKa = 3.83QGSGAEE172 pKa = 4.08ANANQILEE180 pKa = 4.37AAGITYY186 pKa = 10.78DD187 pKa = 5.12DD188 pKa = 3.43ITAEE192 pKa = 4.03FMGFGDD198 pKa = 5.28ASQGLQNGTIDD209 pKa = 3.38AAFIVAGAPTSAIQEE224 pKa = 4.05LGANKK229 pKa = 9.96DD230 pKa = 3.18IRR232 pKa = 11.84VLSLSDD238 pKa = 4.18EE239 pKa = 4.68IISNLTSEE247 pKa = 4.1YY248 pKa = 10.63SYY250 pKa = 10.13YY251 pKa = 9.68TEE253 pKa = 3.99RR254 pKa = 11.84TIKK257 pKa = 10.48ADD259 pKa = 3.95LYY261 pKa = 10.99ADD263 pKa = 3.63YY264 pKa = 10.49GQEE267 pKa = 3.85EE268 pKa = 5.85DD269 pKa = 3.45ITTVAVMAILVVDD282 pKa = 4.06SEE284 pKa = 4.48LPEE287 pKa = 4.05DD288 pKa = 3.75VVYY291 pKa = 11.56NMTKK295 pKa = 11.02DD296 pKa = 3.09MFEE299 pKa = 4.34NKK301 pKa = 9.75DD302 pKa = 3.6ALEE305 pKa = 4.31AAHH308 pKa = 7.15DD309 pKa = 4.24RR310 pKa = 11.84GSDD313 pKa = 3.13ITLEE317 pKa = 4.12TATDD321 pKa = 3.51GMSIDD326 pKa = 4.16LHH328 pKa = 6.36PGAAKK333 pKa = 10.21YY334 pKa = 10.41YY335 pKa = 8.18EE336 pKa = 4.19EE337 pKa = 4.98EE338 pKa = 4.48GVSVDD343 pKa = 3.35

Molecular weight: 36.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9ACP1|A0A4Y9ACP1_9BACI Uncharacterized protein OS=Lentibacillus salicampi OX=175306 GN=E4U82_06335 PE=4 SV=1
MM1 pKa = 7.23IRR3 pKa = 11.84SSIYY7 pKa = 10.32ALSLRR12 pKa = 11.84KK13 pKa = 9.82CINRR17 pKa = 11.84ASTAPTRR24 pKa = 11.84ASTTPTRR31 pKa = 11.84ASTAPTRR38 pKa = 11.84ASTTPTRR45 pKa = 11.84ASTAQTRR52 pKa = 11.84ASTTPTRR59 pKa = 11.84ASTTPTRR66 pKa = 11.84ASTTPTRR73 pKa = 11.84ASTTPTRR80 pKa = 11.84ASTTPTRR87 pKa = 11.84ASTAQTRR94 pKa = 11.84ASTVQNRR101 pKa = 11.84ARR103 pKa = 11.84AASTRR108 pKa = 11.84AGTAQTRR115 pKa = 11.84ARR117 pKa = 11.84TAQTRR122 pKa = 11.84AGTALTRR129 pKa = 11.84AGIAQTRR136 pKa = 11.84ARR138 pKa = 11.84TAQTRR143 pKa = 11.84AGTVQTRR150 pKa = 11.84ASTAQTRR157 pKa = 11.84AGTALTRR164 pKa = 11.84AGIAQTRR171 pKa = 11.84AKK173 pKa = 8.98KK174 pKa = 8.87TKK176 pKa = 10.48LRR178 pKa = 11.84DD179 pKa = 3.55APPLQNQQ186 pKa = 3.21

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3721

0

3721

1050092

14

2509

282.2

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.24 ± 0.035

0.624 ± 0.012

5.963 ± 0.043

7.402 ± 0.046

4.409 ± 0.035

6.989 ± 0.035

2.205 ± 0.019

7.369 ± 0.043

6.235 ± 0.036

9.317 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.964 ± 0.018

4.546 ± 0.031

3.608 ± 0.023

3.992 ± 0.029

4.195 ± 0.034

5.889 ± 0.029

5.606 ± 0.027

6.884 ± 0.03

1.051 ± 0.016

3.511 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski