Amino acid dipepetide frequency for gamma proteobacterium IMCC2047

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.122AlaAla: 9.122 ± 0.159
1.031AlaCys: 1.031 ± 0.044
5.526AlaAsp: 5.526 ± 0.109
6.761AlaGlu: 6.761 ± 0.121
3.351AlaPhe: 3.351 ± 0.083
7.097AlaGly: 7.097 ± 0.124
1.89AlaHis: 1.89 ± 0.067
5.514AlaIle: 5.514 ± 0.095
4.309AlaLys: 4.309 ± 0.102
10.261AlaLeu: 10.261 ± 0.173
2.756AlaMet: 2.756 ± 0.077
3.433AlaAsn: 3.433 ± 0.083
3.076AlaPro: 3.076 ± 0.079
4.081AlaGln: 4.081 ± 0.099
4.582AlaArg: 4.582 ± 0.093
5.395AlaSer: 5.395 ± 0.093
4.468AlaThr: 4.468 ± 0.102
6.248AlaVal: 6.248 ± 0.122
1.013AlaTrp: 1.013 ± 0.047
2.35AlaTyr: 2.35 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.903CysAla: 0.903 ± 0.037
0.222CysCys: 0.222 ± 0.021
0.715CysAsp: 0.715 ± 0.038
0.679CysGlu: 0.679 ± 0.039
0.485CysPhe: 0.485 ± 0.031
0.966CysGly: 0.966 ± 0.046
0.414CysHis: 0.414 ± 0.028
0.534CysIle: 0.534 ± 0.032
0.455CysLys: 0.455 ± 0.029
1.139CysLeu: 1.139 ± 0.056
0.289CysMet: 0.289 ± 0.025
0.336CysAsn: 0.336 ± 0.029
0.589CysPro: 0.589 ± 0.033
0.52CysGln: 0.52 ± 0.033
0.632CysArg: 0.632 ± 0.034
0.673CysSer: 0.673 ± 0.038
0.475CysThr: 0.475 ± 0.032
0.736CysVal: 0.736 ± 0.031
0.153CysTrp: 0.153 ± 0.017
0.334CysTyr: 0.334 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
4.859AspAla: 4.859 ± 0.103
0.618AspCys: 0.618 ± 0.037
3.386AspAsp: 3.386 ± 0.085
4.215AspGlu: 4.215 ± 0.116
2.458AspPhe: 2.458 ± 0.072
3.669AspGly: 3.669 ± 0.095
1.325AspHis: 1.325 ± 0.049
3.783AspIle: 3.783 ± 0.092
2.909AspLys: 2.909 ± 0.091
5.467AspLeu: 5.467 ± 0.112
1.576AspMet: 1.576 ± 0.055
2.216AspAsn: 2.216 ± 0.064
2.102AspPro: 2.102 ± 0.054
2.538AspGln: 2.538 ± 0.08
2.817AspArg: 2.817 ± 0.079
3.057AspSer: 3.057 ± 0.076
2.625AspThr: 2.625 ± 0.082
3.967AspVal: 3.967 ± 0.087
0.785AspTrp: 0.785 ± 0.041
1.955AspTyr: 1.955 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
6.229GluAla: 6.229 ± 0.127
0.52GluCys: 0.52 ± 0.035
2.831GluAsp: 2.831 ± 0.084
4.069GluGlu: 4.069 ± 0.116
2.367GluPhe: 2.367 ± 0.07
3.973GluGly: 3.973 ± 0.096
1.602GluHis: 1.602 ± 0.063
3.991GluIle: 3.991 ± 0.09
3.759GluLys: 3.759 ± 0.09
7.099GluLeu: 7.099 ± 0.114
1.745GluMet: 1.745 ± 0.056
2.581GluAsn: 2.581 ± 0.072
2.309GluPro: 2.309 ± 0.065
4.219GluGln: 4.219 ± 0.095
3.916GluArg: 3.916 ± 0.088
3.6GluSer: 3.6 ± 0.07
3.214GluThr: 3.214 ± 0.092
4.505GluVal: 4.505 ± 0.107
0.681GluTrp: 0.681 ± 0.034
1.461GluTyr: 1.461 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
3.298PheAla: 3.298 ± 0.088
0.538PheCys: 0.538 ± 0.032
2.786PheAsp: 2.786 ± 0.077
2.57PheGlu: 2.57 ± 0.073
1.83PhePhe: 1.83 ± 0.078
2.892PheGly: 2.892 ± 0.079
0.828PheHis: 0.828 ± 0.04
2.46PheIle: 2.46 ± 0.079
1.853PheLys: 1.853 ± 0.054
3.406PheLeu: 3.406 ± 0.094
1.09PheMet: 1.09 ± 0.048
1.7PheAsn: 1.7 ± 0.06
1.394PhePro: 1.394 ± 0.055
1.327PheGln: 1.327 ± 0.054
1.755PheArg: 1.755 ± 0.058
2.98PheSer: 2.98 ± 0.08
2.02PheThr: 2.02 ± 0.065
2.815PheVal: 2.815 ± 0.083
0.542PheTrp: 0.542 ± 0.038
1.26PheTyr: 1.26 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.703GlyAla: 5.703 ± 0.131
1.019GlyCys: 1.019 ± 0.049
3.971GlyAsp: 3.971 ± 0.082
4.543GlyGlu: 4.543 ± 0.098
3.284GlyPhe: 3.284 ± 0.083
5.0GlyGly: 5.0 ± 0.114
1.767GlyHis: 1.767 ± 0.066
4.49GlyIle: 4.49 ± 0.1
3.695GlyLys: 3.695 ± 0.097
7.532GlyLeu: 7.532 ± 0.119
2.106GlyMet: 2.106 ± 0.061
2.475GlyAsn: 2.475 ± 0.063
2.018GlyPro: 2.018 ± 0.07
2.866GlyGln: 2.866 ± 0.077
3.62GlyArg: 3.62 ± 0.089
4.14GlySer: 4.14 ± 0.097
3.339GlyThr: 3.339 ± 0.084
5.342GlyVal: 5.342 ± 0.111
1.04GlyTrp: 1.04 ± 0.048
2.393GlyTyr: 2.393 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
1.775HisAla: 1.775 ± 0.058
0.353HisCys: 0.353 ± 0.027
1.237HisAsp: 1.237 ± 0.05
1.247HisGlu: 1.247 ± 0.05
1.029HisPhe: 1.029 ± 0.048
1.673HisGly: 1.673 ± 0.059
0.726HisHis: 0.726 ± 0.04
1.447HisIle: 1.447 ± 0.062
0.978HisLys: 0.978 ± 0.05
2.391HisLeu: 2.391 ± 0.071
0.552HisMet: 0.552 ± 0.034
0.905HisAsn: 0.905 ± 0.04
1.197HisPro: 1.197 ± 0.051
1.166HisGln: 1.166 ± 0.049
1.292HisArg: 1.292 ± 0.048
1.413HisSer: 1.413 ± 0.055
1.101HisThr: 1.101 ± 0.046
1.402HisVal: 1.402 ± 0.051
0.43HisTrp: 0.43 ± 0.03
0.899HisTyr: 0.899 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
6.084IleAla: 6.084 ± 0.112
0.648IleCys: 0.648 ± 0.036
4.128IleAsp: 4.128 ± 0.094
4.574IleGlu: 4.574 ± 0.117
2.106IlePhe: 2.106 ± 0.074
4.297IleGly: 4.297 ± 0.11
1.127IleHis: 1.127 ± 0.054
3.274IleIle: 3.274 ± 0.083
3.161IleLys: 3.161 ± 0.072
4.965IleLeu: 4.965 ± 0.11
1.231IleMet: 1.231 ± 0.047
2.929IleAsn: 2.929 ± 0.082
2.493IlePro: 2.493 ± 0.076
2.042IleGln: 2.042 ± 0.064
3.004IleArg: 3.004 ± 0.088
4.091IleSer: 4.091 ± 0.084
3.28IleThr: 3.28 ± 0.079
3.634IleVal: 3.634 ± 0.087
0.491IleTrp: 0.491 ± 0.034
1.508IleTyr: 1.508 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.713LysAla: 4.713 ± 0.11
0.324LysCys: 0.324 ± 0.03
2.405LysAsp: 2.405 ± 0.08
2.899LysGlu: 2.899 ± 0.08
1.398LysPhe: 1.398 ± 0.056
3.233LysGly: 3.233 ± 0.084
1.307LysHis: 1.307 ± 0.051
2.742LysIle: 2.742 ± 0.066
2.866LysLys: 2.866 ± 0.095
4.994LysLeu: 4.994 ± 0.099
1.307LysMet: 1.307 ± 0.046
2.038LysAsn: 2.038 ± 0.06
2.623LysPro: 2.623 ± 0.082
2.674LysGln: 2.674 ± 0.089
2.905LysArg: 2.905 ± 0.09
2.819LysSer: 2.819 ± 0.073
2.678LysThr: 2.678 ± 0.074
3.5LysVal: 3.5 ± 0.084
0.442LysTrp: 0.442 ± 0.026
1.082LysTyr: 1.082 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
10.457LeuAla: 10.457 ± 0.164
1.25LeuCys: 1.25 ± 0.054
5.819LeuAsp: 5.819 ± 0.107
6.517LeuGlu: 6.517 ± 0.123
4.144LeuPhe: 4.144 ± 0.115
7.091LeuGly: 7.091 ± 0.134
2.134LeuHis: 2.134 ± 0.056
5.97LeuIle: 5.97 ± 0.125
5.707LeuLys: 5.707 ± 0.124
11.688LeuLeu: 11.688 ± 0.22
2.856LeuMet: 2.856 ± 0.077
4.236LeuAsn: 4.236 ± 0.094
5.024LeuPro: 5.024 ± 0.107
4.601LeuGln: 4.601 ± 0.096
5.4LeuArg: 5.4 ± 0.101
7.448LeuSer: 7.448 ± 0.138
5.624LeuThr: 5.624 ± 0.117
7.267LeuVal: 7.267 ± 0.137
1.095LeuTrp: 1.095 ± 0.055
2.485LeuTyr: 2.485 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.713MetAla: 2.713 ± 0.066
0.228MetCys: 0.228 ± 0.02
1.311MetAsp: 1.311 ± 0.05
1.319MetGlu: 1.319 ± 0.05
0.807MetPhe: 0.807 ± 0.044
1.963MetGly: 1.963 ± 0.057
0.512MetHis: 0.512 ± 0.03
1.543MetIle: 1.543 ± 0.062
1.37MetLys: 1.37 ± 0.05
2.917MetLeu: 2.917 ± 0.085
0.821MetMet: 0.821 ± 0.044
0.907MetAsn: 0.907 ± 0.04
1.402MetPro: 1.402 ± 0.056
1.247MetGln: 1.247 ± 0.048
1.388MetArg: 1.388 ± 0.055
1.824MetSer: 1.824 ± 0.062
1.639MetThr: 1.639 ± 0.052
1.949MetVal: 1.949 ± 0.067
0.177MetTrp: 0.177 ± 0.018
0.524MetTyr: 0.524 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.333AsnAla: 3.333 ± 0.096
0.471AsnCys: 0.471 ± 0.031
2.073AsnAsp: 2.073 ± 0.055
2.263AsnGlu: 2.263 ± 0.07
1.423AsnPhe: 1.423 ± 0.053
2.689AsnGly: 2.689 ± 0.082
0.872AsnHis: 0.872 ± 0.043
2.348AsnIle: 2.348 ± 0.063
1.93AsnLys: 1.93 ± 0.056
3.889AsnLeu: 3.889 ± 0.102
1.007AsnMet: 1.007 ± 0.046
1.781AsnAsn: 1.781 ± 0.063
2.044AsnPro: 2.044 ± 0.071
1.625AsnGln: 1.625 ± 0.059
2.106AsnArg: 2.106 ± 0.062
2.434AsnSer: 2.434 ± 0.075
2.034AsnThr: 2.034 ± 0.057
2.397AsnVal: 2.397 ± 0.076
0.601AsnTrp: 0.601 ± 0.035
1.146AsnTyr: 1.146 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
3.963ProAla: 3.963 ± 0.101
0.379ProCys: 0.379 ± 0.027
2.672ProAsp: 2.672 ± 0.073
3.618ProGlu: 3.618 ± 0.089
1.641ProPhe: 1.641 ± 0.059
3.102ProGly: 3.102 ± 0.073
0.852ProHis: 0.852 ± 0.037
2.163ProIle: 2.163 ± 0.067
1.832ProLys: 1.832 ± 0.072
4.319ProLeu: 4.319 ± 0.097
1.088ProMet: 1.088 ± 0.043
1.437ProAsn: 1.437 ± 0.052
1.347ProPro: 1.347 ± 0.053
1.706ProGln: 1.706 ± 0.061
1.598ProArg: 1.598 ± 0.062
2.419ProSer: 2.419 ± 0.072
1.994ProThr: 1.994 ± 0.066
3.634ProVal: 3.634 ± 0.095
0.516ProTrp: 0.516 ± 0.029
1.164ProTyr: 1.164 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
4.863GlnAla: 4.863 ± 0.114
0.387GlnCys: 0.387 ± 0.028
1.873GlnAsp: 1.873 ± 0.065
2.285GlnGlu: 2.285 ± 0.067
1.676GlnPhe: 1.676 ± 0.053
2.974GlnGly: 2.974 ± 0.079
1.37GlnHis: 1.37 ± 0.047
2.397GlnIle: 2.397 ± 0.07
1.894GlnLys: 1.894 ± 0.069
5.885GlnLeu: 5.885 ± 0.139
1.025GlnMet: 1.025 ± 0.044
1.529GlnAsn: 1.529 ± 0.057
2.104GlnPro: 2.104 ± 0.07
3.936GlnGln: 3.936 ± 0.123
3.131GlnArg: 3.131 ± 0.074
2.515GlnSer: 2.515 ± 0.074
2.112GlnThr: 2.112 ± 0.065
3.253GlnVal: 3.253 ± 0.095
0.607GlnTrp: 0.607 ± 0.033
1.09GlnTyr: 1.09 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
4.315ArgAla: 4.315 ± 0.098
0.616ArgCys: 0.616 ± 0.035
2.972ArgAsp: 2.972 ± 0.079
3.557ArgGlu: 3.557 ± 0.092
2.448ArgPhe: 2.448 ± 0.074
3.1ArgGly: 3.1 ± 0.074
1.408ArgHis: 1.408 ± 0.053
3.259ArgIle: 3.259 ± 0.08
2.54ArgLys: 2.54 ± 0.069
5.879ArgLeu: 5.879 ± 0.11
1.411ArgMet: 1.411 ± 0.05
2.038ArgAsn: 2.038 ± 0.055
1.857ArgPro: 1.857 ± 0.055
2.782ArgGln: 2.782 ± 0.08
2.998ArgArg: 2.998 ± 0.082
3.047ArgSer: 3.047 ± 0.075
2.218ArgThr: 2.218 ± 0.061
3.812ArgVal: 3.812 ± 0.092
0.836ArgTrp: 0.836 ± 0.04
1.824ArgTyr: 1.824 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
5.671SerAla: 5.671 ± 0.105
0.677SerCys: 0.677 ± 0.043
3.441SerAsp: 3.441 ± 0.083
3.897SerGlu: 3.897 ± 0.098
2.548SerPhe: 2.548 ± 0.07
4.919SerGly: 4.919 ± 0.103
1.512SerHis: 1.512 ± 0.047
3.618SerIle: 3.618 ± 0.078
2.86SerLys: 2.86 ± 0.077
6.826SerLeu: 6.826 ± 0.13
1.737SerMet: 1.737 ± 0.058
2.344SerAsn: 2.344 ± 0.067
2.499SerPro: 2.499 ± 0.07
2.668SerGln: 2.668 ± 0.076
3.39SerArg: 3.39 ± 0.081
4.109SerSer: 4.109 ± 0.098
3.009SerThr: 3.009 ± 0.089
4.081SerVal: 4.081 ± 0.097
0.793SerTrp: 0.793 ± 0.04
1.588SerTyr: 1.588 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
4.645ThrAla: 4.645 ± 0.096
0.497ThrCys: 0.497 ± 0.038
2.882ThrAsp: 2.882 ± 0.073
2.99ThrGlu: 2.99 ± 0.081
1.814ThrPhe: 1.814 ± 0.057
4.268ThrGly: 4.268 ± 0.096
1.17ThrHis: 1.17 ± 0.048
2.78ThrIle: 2.78 ± 0.071
1.845ThrLys: 1.845 ± 0.061
6.162ThrLeu: 6.162 ± 0.131
1.029ThrMet: 1.029 ± 0.046
1.741ThrAsn: 1.741 ± 0.06
2.821ThrPro: 2.821 ± 0.076
2.081ThrGln: 2.081 ± 0.06
2.46ThrArg: 2.46 ± 0.076
2.939ThrSer: 2.939 ± 0.075
2.552ThrThr: 2.552 ± 0.075
3.647ThrVal: 3.647 ± 0.092
0.399ThrTrp: 0.399 ± 0.029
1.233ThrTyr: 1.233 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.604ValAla: 6.604 ± 0.125
0.893ValCys: 0.893 ± 0.041
4.238ValAsp: 4.238 ± 0.095
4.58ValGlu: 4.58 ± 0.108
2.764ValPhe: 2.764 ± 0.062
4.812ValGly: 4.812 ± 0.113
1.319ValHis: 1.319 ± 0.057
4.666ValIle: 4.666 ± 0.089
3.455ValLys: 3.455 ± 0.088
7.016ValLeu: 7.016 ± 0.133
2.026ValMet: 2.026 ± 0.06
2.623ValAsn: 2.623 ± 0.076
2.772ValPro: 2.772 ± 0.077
2.263ValGln: 2.263 ± 0.063
3.392ValArg: 3.392 ± 0.083
4.937ValSer: 4.937 ± 0.096
3.855ValThr: 3.855 ± 0.081
5.451ValVal: 5.451 ± 0.118
0.687ValTrp: 0.687 ± 0.038
1.779ValTyr: 1.779 ± 0.071
0.0ValXaa: 0.0 ± 0.0
Trp
0.891TrpAla: 0.891 ± 0.042
0.196TrpCys: 0.196 ± 0.018
0.563TrpAsp: 0.563 ± 0.037
0.522TrpGlu: 0.522 ± 0.029
0.514TrpPhe: 0.514 ± 0.034
0.77TrpGly: 0.77 ± 0.043
0.377TrpHis: 0.377 ± 0.026
0.677TrpIle: 0.677 ± 0.031
0.375TrpLys: 0.375 ± 0.025
1.745TrpLeu: 1.745 ± 0.067
0.302TrpMet: 0.302 ± 0.026
0.351TrpAsn: 0.351 ± 0.028
0.536TrpPro: 0.536 ± 0.037
0.915TrpGln: 0.915 ± 0.045
0.75TrpArg: 0.75 ± 0.039
0.618TrpSer: 0.618 ± 0.034
0.42TrpThr: 0.42 ± 0.032
0.872TrpVal: 0.872 ± 0.043
0.214TrpTrp: 0.214 ± 0.023
0.294TrpTyr: 0.294 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.124TyrAla: 2.124 ± 0.066
0.381TyrCys: 0.381 ± 0.029
1.5TyrAsp: 1.5 ± 0.056
1.567TyrGlu: 1.567 ± 0.055
1.254TyrPhe: 1.254 ± 0.049
1.857TyrGly: 1.857 ± 0.066
0.699TyrHis: 0.699 ± 0.044
1.409TyrIle: 1.409 ± 0.048
1.156TyrLys: 1.156 ± 0.046
3.235TyrLeu: 3.235 ± 0.083
0.605TyrMet: 0.605 ± 0.038
0.866TyrAsn: 0.866 ± 0.042
1.237TyrPro: 1.237 ± 0.045
1.649TyrGln: 1.649 ± 0.056
1.781TyrArg: 1.781 ± 0.056
1.733TyrSer: 1.733 ± 0.06
1.294TyrThr: 1.294 ± 0.051
1.62TyrVal: 1.62 ± 0.062
0.402TyrTrp: 0.402 ± 0.03
0.921TyrTyr: 0.921 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2263 proteins (509404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski