Paramecium bursaria Chlorella virus AR158 (PBCV-AR158)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Chlorovirus; unclassified Chlorovirus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 814 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7RC78|A7RC78_PBCVA Uncharacterized protein C625R OS=Paramecium bursaria Chlorella virus AR158 OX=380598 GN=C625R PE=4 SV=1
MM1 pKa = 7.05YY2 pKa = 10.28FEE4 pKa = 4.81YY5 pKa = 10.81VLQIFNGSIRR15 pKa = 11.84HH16 pKa = 6.18DD17 pKa = 3.74NNEE20 pKa = 3.63YY21 pKa = 8.27FHH23 pKa = 7.66NYY25 pKa = 8.8FFIKK29 pKa = 10.26KK30 pKa = 9.17FVIFFDD36 pKa = 3.73FDD38 pKa = 3.65GLVIFFDD45 pKa = 4.14FGGLVIFFDD54 pKa = 4.08FDD56 pKa = 3.58EE57 pKa = 4.61LVIFFDD63 pKa = 4.5FGGLVIFFDD72 pKa = 4.21CMIEE76 pKa = 3.83ITLSFFLTEE85 pKa = 3.95QEE87 pKa = 4.19EE88 pKa = 4.76EE89 pKa = 4.0INSILFFKK97 pKa = 10.28TSSIFLFFDD106 pKa = 3.79PAA108 pKa = 3.82

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7RB95|A7RB95_PBCVA Uncharacterized protein C292R OS=Paramecium bursaria Chlorella virus AR158 OX=380598 GN=C292R PE=4 SV=1
MM1 pKa = 7.26VHH3 pKa = 4.71EE4 pKa = 5.11HH5 pKa = 6.24EE6 pKa = 4.41RR7 pKa = 11.84SAYY10 pKa = 7.83ILPRR14 pKa = 11.84KK15 pKa = 9.12RR16 pKa = 11.84HH17 pKa = 5.4SFCQHH22 pKa = 4.0GHH24 pKa = 5.97RR25 pKa = 11.84KK26 pKa = 8.85LVRR29 pKa = 11.84GGRR32 pKa = 11.84LQSEE36 pKa = 4.67DD37 pKa = 2.86EE38 pKa = 4.12GMRR41 pKa = 11.84NFVSSSTRR49 pKa = 11.84RR50 pKa = 11.84QRR52 pKa = 11.84TRR54 pKa = 11.84EE55 pKa = 3.64ILGKK59 pKa = 9.92QRR61 pKa = 11.84GYY63 pKa = 10.88CRR65 pKa = 11.84GRR67 pKa = 11.84SWCEE71 pKa = 3.26SGMGFSCDD79 pKa = 3.22EE80 pKa = 4.12TRR82 pKa = 11.84HH83 pKa = 4.85QRR85 pKa = 11.84LGRR88 pKa = 11.84LPFKK92 pKa = 10.64EE93 pKa = 4.14EE94 pKa = 3.77QLGLL98 pKa = 3.78

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

814

0

814

146454

65

1485

179.9

20.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.194 ± 0.107

2.077 ± 0.085

5.482 ± 0.124

4.997 ± 0.124

5.616 ± 0.124

5.361 ± 0.214

2.458 ± 0.087

7.717 ± 0.105

6.788 ± 0.219

7.573 ± 0.114

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.073

5.471 ± 0.18

4.526 ± 0.146

2.644 ± 0.092

5.67 ± 0.147

7.929 ± 0.127

6.12 ± 0.133

6.729 ± 0.115

1.061 ± 0.038

3.703 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski