Amino acid dipepetide frequency for Arthrobacter phage Rings

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.803AlaAla: 12.803 ± 2.604
0.348AlaCys: 0.348 ± 0.136
6.43AlaAsp: 6.43 ± 0.671
5.503AlaGlu: 5.503 ± 0.771
2.549AlaPhe: 2.549 ± 0.478
7.879AlaGly: 7.879 ± 0.784
2.201AlaHis: 2.201 ± 0.361
5.214AlaIle: 5.214 ± 0.62
5.967AlaLys: 5.967 ± 0.893
8.979AlaLeu: 8.979 ± 0.855
2.549AlaMet: 2.549 ± 0.459
4.171AlaAsn: 4.171 ± 0.593
3.418AlaPro: 3.418 ± 0.426
4.287AlaGln: 4.287 ± 0.631
4.982AlaArg: 4.982 ± 0.586
5.677AlaSer: 5.677 ± 0.531
5.735AlaThr: 5.735 ± 0.598
6.372AlaVal: 6.372 ± 0.601
1.506AlaTrp: 1.506 ± 0.302
2.897AlaTyr: 2.897 ± 0.387
0.0AlaXaa: 0.0 ± 0.0
Cys
0.637CysAla: 0.637 ± 0.259
0.174CysCys: 0.174 ± 0.093
0.348CysAsp: 0.348 ± 0.15
0.232CysGlu: 0.232 ± 0.132
0.174CysPhe: 0.174 ± 0.108
0.695CysGly: 0.695 ± 0.203
0.174CysHis: 0.174 ± 0.103
0.174CysIle: 0.174 ± 0.089
0.29CysLys: 0.29 ± 0.162
0.521CysLeu: 0.521 ± 0.165
0.058CysMet: 0.058 ± 0.056
0.29CysAsn: 0.29 ± 0.116
0.637CysPro: 0.637 ± 0.179
0.406CysGln: 0.406 ± 0.138
0.463CysArg: 0.463 ± 0.146
0.521CysSer: 0.521 ± 0.203
0.348CysThr: 0.348 ± 0.137
0.521CysVal: 0.521 ± 0.247
0.116CysTrp: 0.116 ± 0.083
0.348CysTyr: 0.348 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
5.677AspAla: 5.677 ± 0.577
0.406AspCys: 0.406 ± 0.161
3.823AspAsp: 3.823 ± 0.584
4.113AspGlu: 4.113 ± 0.522
2.839AspPhe: 2.839 ± 0.382
4.461AspGly: 4.461 ± 0.464
0.695AspHis: 0.695 ± 0.244
3.476AspIle: 3.476 ± 0.393
3.939AspLys: 3.939 ± 0.532
5.156AspLeu: 5.156 ± 0.519
1.39AspMet: 1.39 ± 0.268
2.665AspAsn: 2.665 ± 0.387
2.723AspPro: 2.723 ± 0.385
1.448AspGln: 1.448 ± 0.396
3.65AspArg: 3.65 ± 0.497
3.592AspSer: 3.592 ± 0.532
3.997AspThr: 3.997 ± 0.477
3.534AspVal: 3.534 ± 0.421
1.274AspTrp: 1.274 ± 0.313
3.07AspTyr: 3.07 ± 0.467
0.0AspXaa: 0.0 ± 0.0
Glu
7.821GluAla: 7.821 ± 0.823
0.463GluCys: 0.463 ± 0.154
4.577GluAsp: 4.577 ± 0.588
4.692GluGlu: 4.692 ± 0.779
3.534GluPhe: 3.534 ± 0.397
4.692GluGly: 4.692 ± 0.616
1.448GluHis: 1.448 ± 0.458
3.823GluIle: 3.823 ± 0.446
3.186GluLys: 3.186 ± 0.459
6.952GluLeu: 6.952 ± 0.713
1.564GluMet: 1.564 ± 0.308
2.549GluAsn: 2.549 ± 0.383
2.665GluPro: 2.665 ± 0.465
2.781GluGln: 2.781 ± 0.434
3.65GluArg: 3.65 ± 0.519
3.418GluSer: 3.418 ± 0.546
3.939GluThr: 3.939 ± 0.464
5.33GluVal: 5.33 ± 0.689
1.159GluTrp: 1.159 ± 0.272
2.317GluTyr: 2.317 ± 0.436
0.0GluXaa: 0.0 ± 0.0
Phe
2.723PheAla: 2.723 ± 0.462
0.406PheCys: 0.406 ± 0.142
3.07PheAsp: 3.07 ± 0.426
2.028PheGlu: 2.028 ± 0.353
1.332PhePhe: 1.332 ± 0.197
2.781PheGly: 2.781 ± 0.381
1.043PheHis: 1.043 ± 0.209
1.622PheIle: 1.622 ± 0.339
1.506PheLys: 1.506 ± 0.265
1.796PheLeu: 1.796 ± 0.285
0.927PheMet: 0.927 ± 0.302
1.448PheAsn: 1.448 ± 0.305
0.985PhePro: 0.985 ± 0.245
1.854PheGln: 1.854 ± 0.354
1.68PheArg: 1.68 ± 0.304
2.491PheSer: 2.491 ± 0.345
2.665PheThr: 2.665 ± 0.34
3.128PheVal: 3.128 ± 0.436
0.174PheTrp: 0.174 ± 0.091
0.695PheTyr: 0.695 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
6.488GlyAla: 6.488 ± 0.781
0.406GlyCys: 0.406 ± 0.192
4.345GlyAsp: 4.345 ± 0.555
4.519GlyGlu: 4.519 ± 0.459
3.012GlyPhe: 3.012 ± 0.346
5.967GlyGly: 5.967 ± 0.614
1.332GlyHis: 1.332 ± 0.252
3.128GlyIle: 3.128 ± 0.577
4.634GlyLys: 4.634 ± 0.586
6.141GlyLeu: 6.141 ± 0.479
1.68GlyMet: 1.68 ± 0.275
4.113GlyAsn: 4.113 ± 0.623
2.607GlyPro: 2.607 ± 0.473
2.781GlyGln: 2.781 ± 0.338
2.723GlyArg: 2.723 ± 0.426
5.04GlySer: 5.04 ± 0.697
5.793GlyThr: 5.793 ± 0.659
6.72GlyVal: 6.72 ± 0.646
1.39GlyTrp: 1.39 ± 0.291
3.244GlyTyr: 3.244 ± 0.496
0.0GlyXaa: 0.0 ± 0.0
His
1.159HisAla: 1.159 ± 0.258
0.232HisCys: 0.232 ± 0.125
0.985HisAsp: 0.985 ± 0.262
1.448HisGlu: 1.448 ± 0.294
0.695HisPhe: 0.695 ± 0.194
1.217HisGly: 1.217 ± 0.231
0.521HisHis: 0.521 ± 0.189
1.217HisIle: 1.217 ± 0.297
0.985HisLys: 0.985 ± 0.305
1.564HisLeu: 1.564 ± 0.273
0.579HisMet: 0.579 ± 0.184
0.695HisAsn: 0.695 ± 0.179
1.043HisPro: 1.043 ± 0.218
0.463HisGln: 0.463 ± 0.176
1.159HisArg: 1.159 ± 0.257
1.101HisSer: 1.101 ± 0.298
1.332HisThr: 1.332 ± 0.238
1.622HisVal: 1.622 ± 0.277
0.348HisTrp: 0.348 ± 0.133
0.521HisTyr: 0.521 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
5.04IleAla: 5.04 ± 0.501
0.579IleCys: 0.579 ± 0.183
4.055IleAsp: 4.055 ± 0.406
4.75IleGlu: 4.75 ± 0.689
1.332IlePhe: 1.332 ± 0.261
3.476IleGly: 3.476 ± 0.589
0.579IleHis: 0.579 ± 0.182
2.375IleIle: 2.375 ± 0.415
2.954IleLys: 2.954 ± 0.463
2.607IleLeu: 2.607 ± 0.305
1.159IleMet: 1.159 ± 0.236
2.086IleAsn: 2.086 ± 0.346
2.259IlePro: 2.259 ± 0.388
1.854IleGln: 1.854 ± 0.453
3.07IleArg: 3.07 ± 0.356
2.723IleSer: 2.723 ± 0.431
3.418IleThr: 3.418 ± 0.457
3.939IleVal: 3.939 ± 0.401
0.869IleTrp: 0.869 ± 0.254
2.086IleTyr: 2.086 ± 0.319
0.0IleXaa: 0.0 ± 0.0
Lys
6.314LysAla: 6.314 ± 0.599
0.232LysCys: 0.232 ± 0.114
2.549LysAsp: 2.549 ± 0.363
3.534LysGlu: 3.534 ± 0.524
1.564LysPhe: 1.564 ± 0.301
3.592LysGly: 3.592 ± 0.422
1.39LysHis: 1.39 ± 0.302
3.012LysIle: 3.012 ± 0.439
2.897LysLys: 2.897 ± 0.396
4.75LysLeu: 4.75 ± 0.433
1.506LysMet: 1.506 ± 0.253
2.491LysAsn: 2.491 ± 0.338
1.854LysPro: 1.854 ± 0.284
2.201LysGln: 2.201 ± 0.438
2.433LysArg: 2.433 ± 0.428
3.186LysSer: 3.186 ± 0.474
4.113LysThr: 4.113 ± 0.365
3.997LysVal: 3.997 ± 0.488
1.101LysTrp: 1.101 ± 0.255
1.39LysTyr: 1.39 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
8.516LeuAla: 8.516 ± 0.897
0.406LeuCys: 0.406 ± 0.152
4.866LeuAsp: 4.866 ± 0.517
5.909LeuGlu: 5.909 ± 0.689
2.259LeuPhe: 2.259 ± 0.37
5.388LeuGly: 5.388 ± 0.586
1.854LeuHis: 1.854 ± 0.353
3.881LeuIle: 3.881 ± 0.436
3.765LeuLys: 3.765 ± 0.382
5.445LeuLeu: 5.445 ± 0.591
2.201LeuMet: 2.201 ± 0.352
3.36LeuAsn: 3.36 ± 0.434
3.36LeuPro: 3.36 ± 0.401
2.954LeuGln: 2.954 ± 0.368
4.171LeuArg: 4.171 ± 0.574
4.924LeuSer: 4.924 ± 0.592
5.909LeuThr: 5.909 ± 0.655
5.156LeuVal: 5.156 ± 0.545
1.68LeuTrp: 1.68 ± 0.386
1.912LeuTyr: 1.912 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
2.665MetAla: 2.665 ± 0.445
0.232MetCys: 0.232 ± 0.117
0.753MetAsp: 0.753 ± 0.18
1.622MetGlu: 1.622 ± 0.347
0.695MetPhe: 0.695 ± 0.207
1.622MetGly: 1.622 ± 0.297
0.463MetHis: 0.463 ± 0.165
1.101MetIle: 1.101 ± 0.265
1.159MetLys: 1.159 ± 0.256
1.448MetLeu: 1.448 ± 0.346
0.463MetMet: 0.463 ± 0.154
0.753MetAsn: 0.753 ± 0.173
1.217MetPro: 1.217 ± 0.277
0.927MetGln: 0.927 ± 0.314
2.086MetArg: 2.086 ± 0.351
2.259MetSer: 2.259 ± 0.364
1.506MetThr: 1.506 ± 0.3
1.506MetVal: 1.506 ± 0.256
0.174MetTrp: 0.174 ± 0.086
0.811MetTyr: 0.811 ± 0.2
0.0MetXaa: 0.0 ± 0.0
Asn
4.055AsnAla: 4.055 ± 0.5
0.116AsnCys: 0.116 ± 0.087
2.665AsnAsp: 2.665 ± 0.358
2.781AsnGlu: 2.781 ± 0.387
1.101AsnPhe: 1.101 ± 0.209
3.765AsnGly: 3.765 ± 0.392
0.579AsnHis: 0.579 ± 0.174
1.97AsnIle: 1.97 ± 0.324
2.375AsnLys: 2.375 ± 0.551
3.36AsnLeu: 3.36 ± 0.527
0.869AsnMet: 0.869 ± 0.233
2.028AsnAsn: 2.028 ± 0.432
2.781AsnPro: 2.781 ± 0.342
1.332AsnGln: 1.332 ± 0.343
2.723AsnArg: 2.723 ± 0.392
2.723AsnSer: 2.723 ± 0.448
2.086AsnThr: 2.086 ± 0.339
3.708AsnVal: 3.708 ± 0.593
0.521AsnTrp: 0.521 ± 0.165
2.028AsnTyr: 2.028 ± 0.454
0.0AsnXaa: 0.0 ± 0.0
Pro
3.592ProAla: 3.592 ± 0.548
0.406ProCys: 0.406 ± 0.172
3.012ProAsp: 3.012 ± 0.459
4.577ProGlu: 4.577 ± 0.485
1.39ProPhe: 1.39 ± 0.246
2.143ProGly: 2.143 ± 0.41
0.695ProHis: 0.695 ± 0.21
1.68ProIle: 1.68 ± 0.289
2.665ProLys: 2.665 ± 0.461
2.954ProLeu: 2.954 ± 0.434
0.927ProMet: 0.927 ± 0.238
1.854ProAsn: 1.854 ± 0.36
1.622ProPro: 1.622 ± 0.367
0.985ProGln: 0.985 ± 0.24
1.854ProArg: 1.854 ± 0.31
2.491ProSer: 2.491 ± 0.343
2.665ProThr: 2.665 ± 0.39
3.128ProVal: 3.128 ± 0.483
0.927ProTrp: 0.927 ± 0.291
1.622ProTyr: 1.622 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
3.765GlnAla: 3.765 ± 0.514
0.463GlnCys: 0.463 ± 0.159
1.738GlnAsp: 1.738 ± 0.289
3.128GlnGlu: 3.128 ± 0.489
1.738GlnPhe: 1.738 ± 0.322
2.317GlnGly: 2.317 ± 0.315
0.579GlnHis: 0.579 ± 0.174
2.028GlnIle: 2.028 ± 0.385
1.68GlnLys: 1.68 ± 0.325
2.781GlnLeu: 2.781 ± 0.369
0.985GlnMet: 0.985 ± 0.148
1.68GlnAsn: 1.68 ± 0.374
0.869GlnPro: 0.869 ± 0.207
2.028GlnGln: 2.028 ± 0.374
1.796GlnArg: 1.796 ± 0.321
2.549GlnSer: 2.549 ± 0.422
2.549GlnThr: 2.549 ± 0.412
2.433GlnVal: 2.433 ± 0.396
0.811GlnTrp: 0.811 ± 0.265
1.39GlnTyr: 1.39 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
4.113ArgAla: 4.113 ± 0.624
0.348ArgCys: 0.348 ± 0.165
3.534ArgAsp: 3.534 ± 0.444
3.302ArgGlu: 3.302 ± 0.455
1.274ArgPhe: 1.274 ± 0.277
3.765ArgGly: 3.765 ± 0.53
1.043ArgHis: 1.043 ± 0.228
3.592ArgIle: 3.592 ± 0.504
3.07ArgLys: 3.07 ± 0.452
4.692ArgLeu: 4.692 ± 0.553
1.448ArgMet: 1.448 ± 0.253
2.259ArgAsn: 2.259 ± 0.382
2.491ArgPro: 2.491 ± 0.406
2.665ArgGln: 2.665 ± 0.465
2.607ArgArg: 2.607 ± 0.399
2.317ArgSer: 2.317 ± 0.331
2.954ArgThr: 2.954 ± 0.325
4.403ArgVal: 4.403 ± 0.488
0.695ArgTrp: 0.695 ± 0.22
1.622ArgTyr: 1.622 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
6.952SerAla: 6.952 ± 0.786
0.406SerCys: 0.406 ± 0.139
5.156SerAsp: 5.156 ± 0.61
4.229SerGlu: 4.229 ± 0.659
2.433SerPhe: 2.433 ± 0.452
5.561SerGly: 5.561 ± 0.875
0.985SerHis: 0.985 ± 0.264
3.302SerIle: 3.302 ± 0.328
2.839SerLys: 2.839 ± 0.359
4.403SerLeu: 4.403 ± 0.44
1.622SerMet: 1.622 ± 0.284
2.723SerAsn: 2.723 ± 0.435
1.796SerPro: 1.796 ± 0.325
1.854SerGln: 1.854 ± 0.397
2.201SerArg: 2.201 ± 0.447
4.634SerSer: 4.634 ± 0.834
3.592SerThr: 3.592 ± 0.507
3.476SerVal: 3.476 ± 0.384
0.811SerTrp: 0.811 ± 0.261
1.68SerTyr: 1.68 ± 0.343
0.0SerXaa: 0.0 ± 0.0
Thr
6.372ThrAla: 6.372 ± 0.776
0.29ThrCys: 0.29 ± 0.15
3.07ThrAsp: 3.07 ± 0.382
4.866ThrGlu: 4.866 ± 0.409
2.897ThrPhe: 2.897 ± 0.472
6.72ThrGly: 6.72 ± 0.663
1.506ThrHis: 1.506 ± 0.333
3.65ThrIle: 3.65 ± 0.472
3.36ThrLys: 3.36 ± 0.396
4.982ThrLeu: 4.982 ± 0.549
0.927ThrMet: 0.927 ± 0.254
3.128ThrAsn: 3.128 ± 0.476
3.012ThrPro: 3.012 ± 0.472
2.028ThrGln: 2.028 ± 0.322
3.823ThrArg: 3.823 ± 0.43
3.823ThrSer: 3.823 ± 0.502
4.519ThrThr: 4.519 ± 0.777
5.33ThrVal: 5.33 ± 0.688
0.811ThrTrp: 0.811 ± 0.262
1.912ThrTyr: 1.912 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
6.257ValAla: 6.257 ± 0.625
0.811ValCys: 0.811 ± 0.252
3.708ValAsp: 3.708 ± 0.402
6.199ValGlu: 6.199 ± 0.666
1.68ValPhe: 1.68 ± 0.31
6.025ValGly: 6.025 ± 0.691
0.927ValHis: 0.927 ± 0.195
3.186ValIle: 3.186 ± 0.403
4.461ValLys: 4.461 ± 0.544
5.156ValLeu: 5.156 ± 0.594
1.738ValMet: 1.738 ± 0.257
3.012ValAsn: 3.012 ± 0.395
3.592ValPro: 3.592 ± 0.496
2.491ValGln: 2.491 ± 0.451
3.997ValArg: 3.997 ± 0.637
4.866ValSer: 4.866 ± 0.543
6.025ValThr: 6.025 ± 0.566
4.692ValVal: 4.692 ± 0.485
1.39ValTrp: 1.39 ± 0.329
2.259ValTyr: 2.259 ± 0.401
0.0ValXaa: 0.0 ± 0.0
Trp
1.796TrpAla: 1.796 ± 0.379
0.174TrpCys: 0.174 ± 0.097
1.217TrpAsp: 1.217 ± 0.251
1.217TrpGlu: 1.217 ± 0.276
0.579TrpPhe: 0.579 ± 0.187
0.985TrpGly: 0.985 ± 0.24
0.232TrpHis: 0.232 ± 0.112
0.753TrpIle: 0.753 ± 0.217
0.637TrpLys: 0.637 ± 0.21
1.738TrpLeu: 1.738 ± 0.407
0.174TrpMet: 0.174 ± 0.099
0.579TrpAsn: 0.579 ± 0.182
0.869TrpPro: 0.869 ± 0.221
0.869TrpGln: 0.869 ± 0.277
1.101TrpArg: 1.101 ± 0.215
0.695TrpSer: 0.695 ± 0.186
0.869TrpThr: 0.869 ± 0.209
1.332TrpVal: 1.332 ± 0.334
0.637TrpTrp: 0.637 ± 0.189
0.637TrpTyr: 0.637 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.723TyrAla: 2.723 ± 0.593
0.232TyrCys: 0.232 ± 0.129
2.028TyrAsp: 2.028 ± 0.28
1.912TyrGlu: 1.912 ± 0.34
1.274TyrPhe: 1.274 ± 0.258
3.012TyrGly: 3.012 ± 0.451
0.637TyrHis: 0.637 ± 0.212
2.028TyrIle: 2.028 ± 0.394
1.738TyrLys: 1.738 ± 0.313
2.491TyrLeu: 2.491 ± 0.391
0.637TyrMet: 0.637 ± 0.21
1.68TyrAsn: 1.68 ± 0.254
1.448TyrPro: 1.448 ± 0.32
1.101TyrGln: 1.101 ± 0.216
2.028TyrArg: 2.028 ± 0.334
1.622TyrSer: 1.622 ± 0.262
3.012TyrThr: 3.012 ± 0.311
2.143TyrVal: 2.143 ± 0.359
0.695TyrTrp: 0.695 ± 0.21
0.985TyrTyr: 0.985 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (17263 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski