Pseudoalteromonas citrea DSM 8771

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; Pseudoalteromonas citrea

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1KNS6|U1KNS6_9GAMM Polysaccharide export protein OS=Pseudoalteromonas citrea DSM 8771 OX=1117314 GN=PCIT_10764 PE=4 SV=1
MM1 pKa = 7.09TRR3 pKa = 11.84QRR5 pKa = 11.84LLGLILVSSSMLSGCGGGSDD25 pKa = 3.91SSSGTTPNTDD35 pKa = 3.03DD36 pKa = 3.92GGSVVVSNSAPVADD50 pKa = 4.85AGNEE54 pKa = 3.79QSVSVGTVVTLSGSQSQDD72 pKa = 2.56ADD74 pKa = 3.36GDD76 pKa = 4.11TLSFEE81 pKa = 4.32WQFITKK87 pKa = 8.96PASSQSSLVNATSSSTTFTPDD108 pKa = 2.3IAGQYY113 pKa = 8.73TVGLVVNDD121 pKa = 3.67GTVNSGQDD129 pKa = 3.25TVTVNAAAVNQMPIANAGSDD149 pKa = 3.59QQVLLGAVITLSGAASQDD167 pKa = 3.34PDD169 pKa = 4.16GDD171 pKa = 3.88TLSYY175 pKa = 10.66QWLFVSKK182 pKa = 10.59PNGSNAALTDD192 pKa = 3.75AQLVNAKK199 pKa = 8.6FTADD203 pKa = 3.06VAGNYY208 pKa = 8.86EE209 pKa = 4.19LGLTVSDD216 pKa = 4.09GQIEE220 pKa = 4.56STQDD224 pKa = 2.69TMTVVAQASTTNQAPIANAGNDD246 pKa = 3.53QTVSVADD253 pKa = 3.59VVTVSGALSSDD264 pKa = 3.24ADD266 pKa = 3.92NDD268 pKa = 4.17TLSYY272 pKa = 10.35AWSVVSKK279 pKa = 9.63PSQSNTVLSSSTSEE293 pKa = 4.3SVQLTPDD300 pKa = 3.16TAGTYY305 pKa = 9.3QLSLVVNDD313 pKa = 4.39GKK315 pKa = 11.51VNSAADD321 pKa = 4.09MITITAEE328 pKa = 4.0AQAGAPTANAGDD340 pKa = 4.11DD341 pKa = 3.56QSVTVGDD348 pKa = 4.97FVALSGSQSTGDD360 pKa = 3.35NLSYY364 pKa = 10.51QWQLVSKK371 pKa = 9.18PDD373 pKa = 3.41NSSAQLSAADD383 pKa = 3.57KK384 pKa = 11.15VSAEE388 pKa = 4.12FNADD392 pKa = 2.75IAGVFEE398 pKa = 4.31VSLTVTQGTSSATDD412 pKa = 3.41MLTVTSSNTSVNITDD427 pKa = 4.79KK428 pKa = 10.96IFTNEE433 pKa = 3.6AVSCRR438 pKa = 11.84NYY440 pKa = 10.03EE441 pKa = 3.55GSYY444 pKa = 10.17FSNVTDD450 pKa = 3.84IKK452 pKa = 10.61RR453 pKa = 11.84AADD456 pKa = 3.52FTGDD460 pKa = 3.28VVLTVGNTTCTITVNSIPNHH480 pKa = 6.38DD481 pKa = 4.5FNDD484 pKa = 3.66NTASFATNASAQNLVYY500 pKa = 10.35NLPITASFATATTSQGIGTTEE521 pKa = 3.82GVLLNGVTLDD531 pKa = 4.45LLPAACYY538 pKa = 10.22DD539 pKa = 4.0VGDD542 pKa = 4.01EE543 pKa = 4.41PIGRR547 pKa = 11.84EE548 pKa = 4.26KK549 pKa = 10.35IGCGPDD555 pKa = 3.4QNDD558 pKa = 3.36NPWRR562 pKa = 11.84YY563 pKa = 10.96DD564 pKa = 3.31PMSSLNTFGTDD575 pKa = 2.3AHH577 pKa = 6.36NAHH580 pKa = 6.12TQPSGKK586 pKa = 9.36YY587 pKa = 8.21HH588 pKa = 4.65YY589 pKa = 9.83HH590 pKa = 6.3GNPVAMFNQTCGSRR604 pKa = 11.84ASAVIGFAADD614 pKa = 4.0GFPIYY619 pKa = 10.52GSCFQDD625 pKa = 2.84ATTGTIRR632 pKa = 11.84KK633 pKa = 7.18ATSSYY638 pKa = 10.03VLKK641 pKa = 10.98NNGGARR647 pKa = 11.84QAVSGYY653 pKa = 4.85TTPVGGTGVVASNNYY668 pKa = 9.66DD669 pKa = 3.29GQFRR673 pKa = 11.84GDD675 pKa = 3.1WEE677 pKa = 4.41YY678 pKa = 11.08QASAGDD684 pKa = 3.79LDD686 pKa = 4.33EE687 pKa = 5.37CNGMTVNGQYY697 pKa = 10.93GYY699 pKa = 10.95YY700 pKa = 8.86VTDD703 pKa = 3.73TFPWVLGCFKK713 pKa = 11.17GNLNGTFTRR722 pKa = 11.84PTNLEE727 pKa = 4.05RR728 pKa = 11.84RR729 pKa = 11.84SHH731 pKa = 4.75SHH733 pKa = 5.78GSEE736 pKa = 3.58GSIAINGG743 pKa = 3.67

Molecular weight: 76.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1JJI4|U1JJI4_9GAMM Anti sigma E (Sigma 24) factor negative regulator OS=Pseudoalteromonas citrea DSM 8771 OX=1117314 GN=PCIT_18274 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVNGRR28 pKa = 11.84KK29 pKa = 9.18VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4457

0

4457

1556861

26

7131

349.3

38.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.441 ± 0.038

1.068 ± 0.012

5.55 ± 0.029

5.898 ± 0.039

4.193 ± 0.025

6.277 ± 0.041

2.515 ± 0.022

6.353 ± 0.028

5.547 ± 0.039

10.437 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.019

4.75 ± 0.027

3.663 ± 0.021

4.988 ± 0.034

4.049 ± 0.027

7.068 ± 0.037

5.694 ± 0.042

6.762 ± 0.033

1.157 ± 0.015

3.301 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski