Streptococcus phage Javan308

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AYL2|A0A4D6AYL2_9CAUD Programmed cell death toxin OS=Streptococcus phage Javan308 OX=2548100 GN=Javan308_0031 PE=4 SV=1
MM1 pKa = 7.74IGVTYY6 pKa = 9.75QEE8 pKa = 3.04IHH10 pKa = 6.73LFVEE14 pKa = 5.07FLKK17 pKa = 10.57EE18 pKa = 3.7QYY20 pKa = 11.13GEE22 pKa = 4.11GRR24 pKa = 11.84PDD26 pKa = 3.58YY27 pKa = 11.03IEE29 pKa = 4.93ALNDD33 pKa = 3.59LDD35 pKa = 4.33GLAKK39 pKa = 10.41AFHH42 pKa = 7.09RR43 pKa = 11.84DD44 pKa = 3.37DD45 pKa = 3.38IEE47 pKa = 5.33RR48 pKa = 11.84FLEE51 pKa = 4.65DD52 pKa = 3.48EE53 pKa = 4.32LL54 pKa = 5.78

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AXW4|A0A4D6AXW4_9CAUD Holin OS=Streptococcus phage Javan308 OX=2548100 GN=Javan308_0066 PE=4 SV=1
MM1 pKa = 7.62NYY3 pKa = 10.35LSRR6 pKa = 11.84VSNRR10 pKa = 11.84LLLAGLRR17 pKa = 11.84NATCLKK23 pKa = 10.52KK24 pKa = 10.31KK25 pKa = 9.42VKK27 pKa = 9.28NWKK30 pKa = 9.98KK31 pKa = 10.36KK32 pKa = 9.32IKK34 pKa = 9.87EE35 pKa = 4.29LEE37 pKa = 4.33DD38 pKa = 3.36EE39 pKa = 4.34

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13193

39

815

194.0

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.443 ± 0.416

0.561 ± 0.084

6.337 ± 0.25

8.406 ± 0.395

4.32 ± 0.199

6.011 ± 0.385

1.448 ± 0.139

6.973 ± 0.242

8.406 ± 0.325

8.186 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.202

5.215 ± 0.233

2.979 ± 0.208

4.146 ± 0.241

4.843 ± 0.286

5.571 ± 0.186

5.806 ± 0.229

6.716 ± 0.262

1.311 ± 0.153

3.911 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski