Syntrophobotulus glycolicus (strain DSM 8271 / FlGlyR)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Syntrophobotulus; Syntrophobotulus glycolicus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0T1M8|F0T1M8_SYNGF Molybdenum-pterin binding protein OS=Syntrophobotulus glycolicus (strain DSM 8271 / FlGlyR) OX=645991 GN=Sgly_3186 PE=4 SV=1
MM1 pKa = 7.43NNLVFNTKK9 pKa = 10.34AEE11 pKa = 4.03EE12 pKa = 4.19LKK14 pKa = 10.39TSIYY18 pKa = 10.63AQAPDD23 pKa = 4.9LSTKK27 pKa = 10.1PLQLDD32 pKa = 3.55ANDD35 pKa = 3.93NLLVAGDD42 pKa = 3.79FTISEE47 pKa = 4.11ITNPVTIANASLTVAGTVTVSEE69 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE91 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE113 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE135 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE157 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE179 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE201 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE223 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE245 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE267 pKa = 4.37VTNPVTIANASLTVAGTVTVSEE289 pKa = 4.37VTNPVTIANASLTVSVGGYY308 pKa = 10.42LFTSDD313 pKa = 5.26LVADD317 pKa = 4.04NPITGTGVLFNNTDD331 pKa = 2.79ISQVKK336 pKa = 7.5TASLLLYY343 pKa = 10.02NQGAVPLTLSLQISPTTTDD362 pKa = 2.99GDD364 pKa = 4.41YY365 pKa = 10.74IDD367 pKa = 5.43DD368 pKa = 4.2PSYY371 pKa = 11.28TNVVLEE377 pKa = 4.39ANEE380 pKa = 4.28KK381 pKa = 10.35KK382 pKa = 10.96YY383 pKa = 10.41MAISSFAHH391 pKa = 5.01YY392 pKa = 10.7VRR394 pKa = 11.84LQYY397 pKa = 11.67LMGGSTGTITAYY409 pKa = 11.06YY410 pKa = 9.4NAQTT414 pKa = 3.9

Molecular weight: 41.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0STS2|F0STS2_SYNGF Uncharacterized protein OS=Syntrophobotulus glycolicus (strain DSM 8271 / FlGlyR) OX=645991 GN=Sgly_1030 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.57MTYY5 pKa = 8.24QPKK8 pKa = 9.39NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.74KK13 pKa = 10.14KK14 pKa = 8.03VHH16 pKa = 5.76GFLSRR21 pKa = 11.84MKK23 pKa = 10.34SATGRR28 pKa = 11.84NVLKK32 pKa = 10.53RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.55GRR39 pKa = 11.84KK40 pKa = 9.03KK41 pKa = 10.09LTVV44 pKa = 3.1

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3105

0

3105

954276

30

4339

307.3

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.971 ± 0.058

1.165 ± 0.015

5.045 ± 0.029

7.083 ± 0.048

4.155 ± 0.036

7.473 ± 0.053

1.745 ± 0.02

7.642 ± 0.046

6.365 ± 0.041

10.208 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.022

4.115 ± 0.028

3.729 ± 0.029

3.634 ± 0.027

4.833 ± 0.041

6.024 ± 0.036

5.197 ± 0.052

6.689 ± 0.039

0.952 ± 0.015

3.411 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski