Salmonella phage SPN1S

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; Salmonella virus SPN1S

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2D0E9|H2D0E9_9CAUD Uncharacterized protein OS=Salmonella phage SPN1S OX=1125653 GN=SPN1S_0013 PE=4 SV=1
MM1 pKa = 7.04KK2 pKa = 9.48TVNMKK7 pKa = 9.57TGTDD11 pKa = 3.47SFVGEE16 pKa = 4.34DD17 pKa = 4.08GKK19 pKa = 10.18PEE21 pKa = 4.15TKK23 pKa = 10.27DD24 pKa = 3.14QYY26 pKa = 11.07PWGLRR31 pKa = 11.84ITLDD35 pKa = 3.44NEE37 pKa = 4.1SLQRR41 pKa = 11.84LGLNAKK47 pKa = 9.35SLPAVGDD54 pKa = 4.16SVSVMAMASVCSVSTRR70 pKa = 11.84TTDD73 pKa = 2.85HH74 pKa = 6.84GEE76 pKa = 3.81DD77 pKa = 3.95SYY79 pKa = 12.32VEE81 pKa = 3.96LQITDD86 pKa = 3.17IGLAPQKK93 pKa = 10.57RR94 pKa = 11.84DD95 pKa = 3.42DD96 pKa = 3.98AKK98 pKa = 10.15EE99 pKa = 4.01LKK101 pKa = 10.45DD102 pKa = 3.15AFYY105 pKa = 10.36PVGEE109 pKa = 4.87DD110 pKa = 3.9DD111 pKa = 4.13

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2D0H1|H2D0H1_9CAUD Putative transcriptional activator OS=Salmonella phage SPN1S OX=1125653 GN=SPN1S_0035 PE=4 SV=1
MM1 pKa = 7.61TNPHH5 pKa = 7.02DD6 pKa = 4.82NICVGSITMVYY17 pKa = 8.71STLRR21 pKa = 11.84RR22 pKa = 11.84GWLAPGGQIIRR33 pKa = 11.84NPLKK37 pKa = 9.89AQRR40 pKa = 11.84IAEE43 pKa = 4.16LMNSKK48 pKa = 9.95KK49 pKa = 10.41VAAA52 pKa = 4.71

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11740

38

937

225.8

25.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.957 ± 0.566

1.014 ± 0.157

6.158 ± 0.227

5.869 ± 0.319

3.458 ± 0.195

7.411 ± 0.343

1.627 ± 0.148

5.537 ± 0.369

5.162 ± 0.305

7.615 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.836 ± 0.268

4.744 ± 0.304

4.003 ± 0.233

4.872 ± 0.41

5.852 ± 0.41

6.388 ± 0.273

5.988 ± 0.328

6.618 ± 0.299

1.67 ± 0.176

3.22 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski