Fusarium oxysporum (strain Fo5176) (Fusarium vascular wilt)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium oxysporum species complex; Fusarium oxysporum; Fusarium oxysporum f. sp. conglutinans

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9G944|F9G944_FUSOF Uncharacterized protein OS=Fusarium oxysporum (strain Fo5176) OX=660025 GN=FOXB_15176 PE=3 SV=1
MM1 pKa = 7.56SDD3 pKa = 3.2SYY5 pKa = 11.86GDD7 pKa = 3.49NSYY10 pKa = 11.36GSSRR14 pKa = 11.84RR15 pKa = 11.84DD16 pKa = 3.15NDD18 pKa = 3.0NDD20 pKa = 3.17NSYY23 pKa = 11.36GSSNRR28 pKa = 11.84DD29 pKa = 2.85NDD31 pKa = 3.33NDD33 pKa = 3.33NSYY36 pKa = 11.3GSSRR40 pKa = 11.84RR41 pKa = 11.84DD42 pKa = 3.15NDD44 pKa = 3.0NDD46 pKa = 3.12NSYY49 pKa = 11.66GSGNNNSSSYY59 pKa = 11.25

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9F7M3|F9F7M3_FUSOF Carboxypeptidase OS=Fusarium oxysporum (strain Fo5176) OX=660025 GN=FOXB_02398 PE=3 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.63RR8 pKa = 11.84HH9 pKa = 4.04TAPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APAHH26 pKa = 4.84SHH28 pKa = 5.33RR29 pKa = 11.84HH30 pKa = 4.03TTTTTTTTTKK40 pKa = 9.5PRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84GMFGGGAGRR53 pKa = 11.84RR54 pKa = 11.84THH56 pKa = 6.46ATTTAPVHH64 pKa = 4.87HH65 pKa = 6.63HH66 pKa = 5.14QRR68 pKa = 11.84RR69 pKa = 11.84PSMKK73 pKa = 10.02DD74 pKa = 2.95KK75 pKa = 11.41VSGALLKK82 pKa = 11.04LKK84 pKa = 10.68GSLTRR89 pKa = 11.84RR90 pKa = 11.84PGVKK94 pKa = 9.89AAGTRR99 pKa = 11.84RR100 pKa = 11.84MRR102 pKa = 11.84GTDD105 pKa = 3.02GRR107 pKa = 11.84GARR110 pKa = 11.84HH111 pKa = 5.87HH112 pKa = 7.23RR113 pKa = 11.84YY114 pKa = 9.44

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17784

0

17784

8238472

8

7453

463.3

51.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.26 ± 0.017

1.345 ± 0.009

5.817 ± 0.013

6.19 ± 0.02

3.809 ± 0.013

6.67 ± 0.017

2.437 ± 0.009

5.188 ± 0.014

5.025 ± 0.017

8.9 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.219 ± 0.007

3.821 ± 0.008

5.842 ± 0.021

4.051 ± 0.015

5.888 ± 0.015

8.084 ± 0.02

6.015 ± 0.022

6.036 ± 0.012

1.569 ± 0.008

2.835 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski